BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030623sawa_F11_e86_11.seq (1494 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 149 7e-37 SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 145 1e-35 SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces... 31 0.41 SPBC15C4.04c |||amino acid permease, unknown 10|Schizosaccharomy... 30 0.95 SPCC188.11 |prp45|cwf13, snw1, SPCC584.08|transcriptional regula... 29 1.3 SPBC16H5.05c |cyp7|cwf27|cyclophilin family peptidyl-prolyl cis-... 27 8.8 SPBC839.10 |usp107|snu71|U1 snRNP-associated protein Usp107|Schi... 27 8.8 SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit ... 27 8.8 >SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces pombe|chr 1|||Manual Length = 270 Score = 149 bits (362), Expect = 7e-37 Identities = 89/190 (46%), Positives = 115/190 (60%), Gaps = 6/190 (3%) Frame = +2 Query: 143 KEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSWRVI 322 +E+ V AKLAEQAERY+ M MK V T EL+ EERNLLSVAYKNV+GARR+SWR++ Sbjct: 6 REDAVYLAKLAEQAERYEGMVENMKSVASTDQELTVEERNLLSVAYKNVIGARRASWRIV 65 Query: 323 SSIEQKTE--GSERKQQMAKEYRVKVEKELREICYDVLCLLDKHLIPKASNPXSKVFLPQ 496 SSIEQK E G+ + ++ KEYR K+E+EL IC D+L +L+KHLIP A++ SKVF + Sbjct: 66 SSIEQKEESKGNTAQVELIKEYRQKIEQELDTICQDILTVLEKHLIPNAASAESKVFYYK 125 Query: 497 NEGRLLQ--VSRXSGHRRNQTFCCRGFTESXPGCFLXS--ARQNCSPHTQXXLXLASNXS 664 +G + G +R + +S G S A +P L LA N S Sbjct: 126 MKGDYYRYLAEFAVGEKRQHS-----ADQSLEGYKAASEIATAELAPTHPIRLGLALNFS 180 Query: 665 XFYXXILNXP 694 FY ILN P Sbjct: 181 VFYYEILNSP 190 >SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces pombe|chr 1|||Manual Length = 270 Score = 145 bits (352), Expect = 1e-35 Identities = 89/192 (46%), Positives = 111/192 (57%), Gaps = 4/192 (2%) Frame = +2 Query: 131 MXVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSS 310 M +E V AKLAEQAERY++M MK+V + +LS EERNLLSVAYKN++GARR+S Sbjct: 1 MSNSRENSVYLAKLAEQAERYEEMVENMKKVACSNDKLSVEERNLLSVAYKNIIGARRAS 60 Query: 311 WRVISSIEQKTE--GSERKQQMAKEYRVKVEKELREICYDVLCLLDKHLIPKASNPXSKV 484 WR+ISSIEQK E G+ R+ + KEYR K+E EL +IC+DVL +L+KHLIP A+ SKV Sbjct: 61 WRIISSIEQKEESRGNTRQAALIKEYRKKIEDELSDICHDVLSVLEKHLIPAATTGESKV 120 Query: 485 FLPQNEGRLLQVSRXSGHRRNQTFCCRGFTESXPGCFLXS--ARQNCSPHTQXXLXLASN 658 F + +G R C S S A P L LA N Sbjct: 121 FYYKMKG---DYYRYLAEFTVGEVCKEAADSSLEAYKAASDIAVAELPPTDPMRLGLALN 177 Query: 659 XSXFYXXILNXP 694 S FY IL+ P Sbjct: 178 FSVFYYEILDSP 189 Score = 41.5 bits (93), Expect = 3e-04 Identities = 19/38 (50%), Positives = 22/38 (57%) Frame = +3 Query: 480 KYSYLKMKGDYYRYLAXVATGETRHSVVEDSQKAYQDA 593 K Y KMKGDYYRYLA GE + S +AY+ A Sbjct: 119 KVFYYKMKGDYYRYLAEFTVGEVCKEAADSSLEAYKAA 156 >SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 3131 Score = 31.1 bits (67), Expect = 0.41 Identities = 23/99 (23%), Positives = 43/99 (43%), Gaps = 2/99 (2%) Frame = +2 Query: 146 EELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSWRVIS 325 EEL+ R + + Y+ MA + E E ++ + LLS Y N +R+ + Sbjct: 3011 EELM-RERFEHLLKAYEKMALMVAEQEEFNAKIEDMALKLLSEKYDNEAYQAELFYRLSN 3069 Query: 326 SIEQKTEGSERKQQMAKEYRVKVEKELREIC--YDVLCL 436 +E+ + EY +E+ L++ C Y+ C+ Sbjct: 3070 CVEKVLHNKISITDLKTEYEEILEQTLKKECKAYERSCI 3108 >SPBC15C4.04c |||amino acid permease, unknown 10|Schizosaccharomyces pombe|chr 2|||Manual Length = 542 Score = 29.9 bits (64), Expect = 0.95 Identities = 19/59 (32%), Positives = 33/59 (55%), Gaps = 3/59 (5%) Frame = +2 Query: 158 QRAKLAEQAERYDDMAAAMKEVT--ETGVELSNEERN-LLSVAYKNVVGARRSSWRVIS 325 ++ K ++ E + ++ + +K V+ ET E+SN+E N LL + YK V S+W S Sbjct: 11 EQGKFNDKEEGFSNLKS-LKHVSHSETDFEVSNDEDNQLLELGYKPVFKREFSTWATFS 68 >SPCC188.11 |prp45|cwf13, snw1, SPCC584.08|transcriptional regulator Prp45|Schizosaccharomyces pombe|chr 3|||Manual Length = 557 Score = 29.5 bits (63), Expect = 1.3 Identities = 27/93 (29%), Positives = 45/93 (48%), Gaps = 3/93 (3%) Frame = +2 Query: 140 DKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSWRV 319 +K+E QR + Q R D M + +G S+ + SV+ + +R S+ Sbjct: 320 EKQEKEQRLFMLAQKAREDRMG---RNAASSGP--SHAKPRSTSVSSEERSRSRAGSFSH 374 Query: 320 ISSIEQKTEGSE---RKQQMAKEYRVKVEKELR 409 S E + E SE R+Q++ +E R + EK+LR Sbjct: 375 HSESENEDEDSEAFRRRQELRRERRRQAEKDLR 407 >SPBC16H5.05c |cyp7|cwf27|cyclophilin family peptidyl-prolyl cis-trans isomerase Cyp7|Schizosaccharomyces pombe|chr 2|||Manual Length = 463 Score = 26.6 bits (56), Expect = 8.8 Identities = 12/39 (30%), Positives = 20/39 (51%) Frame = +2 Query: 281 KNVVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVE 397 KN+ S+ R +SS + K +E + M +Y K+E Sbjct: 350 KNLENDEESTLRALSSFQSKIRNAEDEDVMDSQYGSKIE 388 >SPBC839.10 |usp107|snu71|U1 snRNP-associated protein Usp107|Schizosaccharomyces pombe|chr 2|||Manual Length = 695 Score = 26.6 bits (56), Expect = 8.8 Identities = 30/120 (25%), Positives = 49/120 (40%), Gaps = 3/120 (2%) Frame = +2 Query: 140 DKEELVQRAKLAEQA-ERYDDMAAAMK--EVTETGVELSNEERNLLSVAYKNVVGARRSS 310 D ++RA A QA E+ + + +K E+ +L LL V + + R S Sbjct: 265 DVRSRIERA--ARQAREKNEKLLQNVKTSEIPINAADLEGINPELLPVIEEEIRSFRDQS 322 Query: 311 WRVISSIEQKTEGSERKQQMAKEYRVKVEKELREICYDVLCLLDKHLIPKASNPXSKVFL 490 +K + + KEY K +++LR+ D+ LL KH I + FL Sbjct: 323 ---AMKKREKQRSKDEYASLYKEYTRKEQEKLRKQNDDLQNLLSKHRISRIPMSTVNAFL 379 >SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit Bgs2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1894 Score = 26.6 bits (56), Expect = 8.8 Identities = 11/31 (35%), Positives = 18/31 (58%) Frame = -3 Query: 454 YKVLVQ*TQDIIADFSELLFYFNPIFFCHLL 362 + L Q ++++I +EL F+ F CHLL Sbjct: 1755 FHALSQPSRELICKMTELNFFAADFFLCHLL 1785 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,563,501 Number of Sequences: 5004 Number of extensions: 57449 Number of successful extensions: 146 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 138 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 142 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 834495254 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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