BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030623sawa_F10_e78_12.seq (1416 letters) Database: bee 438 sequences; 146,343 total letters Searching......................................................done Score E Sequences producing significant alignments: (bits) Value DQ026031-1|AAY87890.1| 601|Apis mellifera nicotinic acetylcholi... 29 0.074 DQ026034-1|AAY87893.1| 569|Apis mellifera nicotinic acetylcholi... 27 0.30 DQ026033-1|AAY87892.1| 569|Apis mellifera nicotinic acetylcholi... 27 0.52 DQ026032-1|AAY87891.1| 566|Apis mellifera nicotinic acetylcholi... 24 3.7 AJ968562-1|CAI91546.1| 998|Apis mellifera protein ( Apis mellif... 23 6.4 >DQ026031-1|AAY87890.1| 601|Apis mellifera nicotinic acetylcholine receptor alpha1subunit protein. Length = 601 Score = 29.5 bits (63), Expect = 0.074 Identities = 16/73 (21%), Positives = 34/73 (46%) Frame = +2 Query: 161 C*VRRDNWTRHGYSSFP*GVGRREGNKNVRQNTDRG*NHRCEIFLS*SWDVSEGIANRSL 340 C ++ +WT GY+ + + E + + D + ++S WD+ + A R+ Sbjct: 159 CFMKFGSWTYDGYTVDLRHLAQTEDSNQIEVGIDL-----TDYYISVEWDIIKVPAVRNE 213 Query: 341 AWFVCARRPANDV 379 A+++C P D+ Sbjct: 214 AFYICCEEPYPDI 226 >DQ026034-1|AAY87893.1| 569|Apis mellifera nicotinic acetylcholine receptor alpha4subunit protein. Length = 569 Score = 27.5 bits (58), Expect = 0.30 Identities = 18/76 (23%), Positives = 32/76 (42%) Frame = +2 Query: 152 VWRC*VRRDNWTRHGYSSFP*GVGRREGNKNVRQNTDRG*NHRCEIFLS*SWDVSEGIAN 331 V C ++ +WT G+ + + G+ V D E ++S WD+ E A Sbjct: 164 VQTCVLKLGSWTYDGFKVDLRHMDEKSGSNVVDVGVDLS-----EFYMSVEWDILEVPAV 218 Query: 332 RSLAWFVCARRPANDV 379 R+ ++ C P D+ Sbjct: 219 RNEKFYTCCDEPYLDI 234 >DQ026033-1|AAY87892.1| 569|Apis mellifera nicotinic acetylcholine receptor alpha4subunit protein. Length = 569 Score = 26.6 bits (56), Expect = 0.52 Identities = 17/73 (23%), Positives = 31/73 (42%) Frame = +2 Query: 161 C*VRRDNWTRHGYSSFP*GVGRREGNKNVRQNTDRG*NHRCEIFLS*SWDVSEGIANRSL 340 C ++ +WT G+ + + G+ V D E ++S WD+ E A R+ Sbjct: 167 CVLKFGSWTYDGFKVDLRHMDEKSGSNVVDVGVDLS-----EFYMSVEWDILEVPAVRNE 221 Query: 341 AWFVCARRPANDV 379 ++ C P D+ Sbjct: 222 KFYTCCDEPYLDI 234 >DQ026032-1|AAY87891.1| 566|Apis mellifera nicotinic acetylcholine receptor alpha3subunit protein. Length = 566 Score = 23.8 bits (49), Expect = 3.7 Identities = 10/32 (31%), Positives = 16/32 (50%) Frame = +2 Query: 284 EIFLS*SWDVSEGIANRSLAWFVCARRPANDV 379 E + S WD+ E A R+ ++ C P D+ Sbjct: 199 EFYTSVEWDILEVPAVRNEKFYTCCDEPYLDI 230 >AJ968562-1|CAI91546.1| 998|Apis mellifera protein ( Apis mellifera ORF for hypotheticalprotein. ). Length = 998 Score = 23.0 bits (47), Expect = 6.4 Identities = 9/27 (33%), Positives = 15/27 (55%) Frame = -2 Query: 380 THHLLVAERIQTTLKNDLQYPHSHPTI 300 TH+L+ + + L +DL +HP I Sbjct: 970 THYLVSKDGVSLNLDSDLPVEPTHPII 996 Database: bee Posted date: Oct 23, 2007 1:17 PM Number of letters in database: 146,343 Number of sequences in database: 438 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 282,656 Number of Sequences: 438 Number of extensions: 5652 Number of successful extensions: 10 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 10 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 10 length of database: 146,343 effective HSP length: 60 effective length of database: 120,063 effective search space used: 49345893 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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