BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030623sawa_F03_e22_11.seq (1599 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str... 190 3e-48 At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S... 188 9e-48 At1g21730.1 68414.m02720 kinesin-related protein (MKRP1) Similar... 31 1.6 At2g35640.1 68415.m04371 hydroxyproline-rich glycoprotein family... 30 4.9 At2g18470.1 68415.m02151 protein kinase family protein contains ... 29 6.5 At1g70370.1 68414.m08095 BURP domain-containing protein / polyga... 29 6.5 At4g08380.1 68417.m01384 proline-rich extensin-like family prote... 29 8.6 At1g27740.1 68414.m03390 basic helix-loop-helix (bHLH) family pr... 29 8.6 >At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong similarity to 60S ribosomal protein L1 GB:P49691 Length = 406 Score = 190 bits (462), Expect = 3e-48 Identities = 92/172 (53%), Positives = 111/172 (64%), Gaps = 2/172 (1%) Frame = +2 Query: 128 MSLSVARPLVSVYSEKSEVVAGAS--LPLPFVFKAPIRPDLVNDVHVSMSKNARQPYCVS 301 M+ + ARPLV++ + ++ S + LP V AP+RPD+VN VH +S N+RQPY VS Sbjct: 1 MAAAAARPLVTIQTLDGDMSTDQSSTVVLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVS 60 Query: 302 KEAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWXXXXXXX 481 K+AGHQTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK W Sbjct: 61 KKAGHQTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRV 120 Query: 482 XXXXXXXXXXXXXXXXXXXXLVQARGHIVEKIPELPLVVSDNVXGXHKTKQA 637 LV ARGH +E +PE+PLVVSD+ KT A Sbjct: 121 NVNMKRHAIVSAIAATAVPALVMARGHKIENVPEMPLVVSDSAEAVEKTSAA 172 >At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S roibosomal protein L4, Arabidopsis thaliana, EMBL:CAA79104 Length = 407 Score = 188 bits (458), Expect = 9e-48 Identities = 92/169 (54%), Positives = 108/169 (63%), Gaps = 2/169 (1%) Frame = +2 Query: 137 SVARPLVSVYSEKSEVVAGAS--LPLPFVFKAPIRPDLVNDVHVSMSKNARQPYCVSKEA 310 + ARPLV+V ++ S + LP V AP+RPD+VN VH +S N+RQPY VSK+A Sbjct: 5 AAARPLVTVQGLDGDMSTDQSTTVTLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVSKKA 64 Query: 311 GHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWXXXXXXXXXX 490 GHQTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK W Sbjct: 65 GHQTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVNVN 124 Query: 491 XXXXXXXXXXXXXXXXXLVQARGHIVEKIPELPLVVSDNVXGXHKTKQA 637 LV ARGH +E +PE+PLVVSD+ KT A Sbjct: 125 MKRHAIVSAIAATAVPALVMARGHKIENVPEMPLVVSDSAEAVEKTSAA 173 >At1g21730.1 68414.m02720 kinesin-related protein (MKRP1) Similar to gb|U06698 neuronal kinesin heavy chain from Homo sapiens and contains a PF|00225 Kinesin motor domain. EST gb|AA042507 comes from this gene; identical to cDNA MKRP1 mRNA for kinesin-related protein, GI:16902291, kinesin-related protein [Arabidopsis thaliana] GI:16902292 Length = 890 Score = 31.5 bits (68), Expect = 1.6 Identities = 16/37 (43%), Positives = 22/37 (59%), Gaps = 1/37 (2%) Frame = -1 Query: 258 WTSLTRS-GLMGALNTNGRGSEAPATTSLFSEYTDTK 151 W L +S G+ G L T+G S+A + S S+YT TK Sbjct: 549 WLKLKKSDGVAGTLPTDGNQSQASGSPSSSSKYTQTK 585 >At2g35640.1 68415.m04371 hydroxyproline-rich glycoprotein family protein contains proline-rich extensin domains, INTERPRO:IPR002965 Length = 340 Score = 29.9 bits (64), Expect = 4.9 Identities = 14/49 (28%), Positives = 22/49 (44%) Frame = -1 Query: 471 CQRLHGLVGANIRPPRHMLPNAPCPDLWVPPPRTRGIRATARPVPQDSA 325 CQ+ G V + P H +P L PPP++ + + P P S+ Sbjct: 169 CQQSLGFVAPMMAQPMHQIPTTIVLSLPPPPPQSLSLSLPSPPQPPPSS 217 >At2g18470.1 68415.m02151 protein kinase family protein contains Pfam PF00069: Protein kinase domain Length = 633 Score = 29.5 bits (63), Expect = 6.5 Identities = 14/40 (35%), Positives = 20/40 (50%) Frame = -3 Query: 577 NLLNNMPSSLNEGRYPGRCHGGGHGSTSLP*VHATVPAPP 458 N NN PS ++G R +GG +G P + P+PP Sbjct: 66 NSSNNSPSPPSQGGGGERGNGGNNGGNDTPPSRGSPPSPP 105 >At1g70370.1 68414.m08095 BURP domain-containing protein / polygalacturonase, putative similar to polygalacturonase isoenzyme 1 beta subunit [Lycopersicon esculentum] GI:170480; contains Pfam profile PF03181: BURP domain Length = 626 Score = 29.5 bits (63), Expect = 6.5 Identities = 18/57 (31%), Positives = 25/57 (43%) Frame = -1 Query: 264 DTWTSLTRSGLMGALNTNGRGSEAPATTSLFSEYTDTKGRATDRLISLYQRSYSSKK 94 DT+TS G + N N G+ A F+ Y D K D S Y + +S+K Sbjct: 278 DTFTSYGSDGNVPQNNFNNYGASGNAAVDTFANYRD-KANVGDDSFSSYAKDSNSEK 333 >At4g08380.1 68417.m01384 proline-rich extensin-like family protein contains proline-rich extensin domains, INTERPRO:IPR002965 Length = 437 Score = 29.1 bits (62), Expect = 8.6 Identities = 10/19 (52%), Positives = 12/19 (63%) Frame = -1 Query: 432 PPRHMLPNAPCPDLWVPPP 376 PP P PCPD++ PPP Sbjct: 385 PPYAYSPPPPCPDVYKPPP 403 >At1g27740.1 68414.m03390 basic helix-loop-helix (bHLH) family protein contains Pfam profile: PF00010 helix-loop-helix DNA-binding domain Length = 258 Score = 29.1 bits (62), Expect = 8.6 Identities = 20/54 (37%), Positives = 26/54 (48%) Frame = -1 Query: 216 TNGRGSEAPATTSLFSEYTDTKGRATDRLISLYQRSYSSKKHYRRWQRPSLVPN 55 +N E + TS+ + TKG ATD SLY R K + R +LVPN Sbjct: 150 SNSSDDEKASVTSVKGKTRATKGTATDPQ-SLYARKRREKINERLKTLQNLVPN 202 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 20,069,554 Number of Sequences: 28952 Number of extensions: 334934 Number of successful extensions: 914 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 857 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 911 length of database: 12,070,560 effective HSP length: 84 effective length of database: 9,638,592 effective search space used: 4318089216 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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