Samia BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030623sawa_E11_e85_09.seq
         (1515 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC1450.04 |tef5||translation elongation factor EF-1 beta subun...   156   9e-39
SPBP35G2.10 |mit1||SHREC complex subunit Mit1|Schizosaccharomyce...    29   2.2  

>SPCC1450.04 |tef5||translation elongation factor EF-1 beta subunit
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 214

 Score =  156 bits (378), Expect = 9e-39
 Identities = 85/209 (40%), Positives = 115/209 (55%), Gaps = 1/209 (0%)
 Frame = +2

Query: 122 MALGDVKTAQGLNELNQYLAERSYVSGYTPSQADIKVFEQVGKVP-AASLPHVLRWYSHI 298
           M   D+ +  GL +LN +L ++S++ GY PSQAD  VF+ VG  P  A  P+  RWY  I
Sbjct: 1   MGFSDLTSDAGLKQLNDFLLDKSFIEGYEPSQADAVVFKAVGVAPDTAKYPNGARWYKQI 60

Query: 299 ASYTPAERKTWSEGVSXXXXXXXXXXXXXXSNXXXXXXXXLFGSGXXXXXXXXXXXXXXX 478
           A+Y  A     ++ VS               +        LFGS                
Sbjct: 61  ATYDLATLPGTAKEVSAYGPEGAAAAEEDEID--------LFGS-DEEEDPEAERIKAER 111

Query: 479 LKAYADKKSKKPALIAKSSIILDVKPWDDETDMKEMENQVRTIEMDGLLWGASKLVPVGY 658
           +  Y  KK+ KP  + KS + LDVKPWDDET M E+E  VR+I+MDGL+WG SKLVPVG+
Sbjct: 112 VAEYNKKKAAKPKAVHKSLVTLDVKPWDDETPMDELEKAVRSIQMDGLVWGLSKLVPVGF 171

Query: 659 GINKLQIMCVIEDDKVSVDLLTEKFKNLK 745
           G+NK QI  V+EDDKVS++ L E+ +  +
Sbjct: 172 GVNKFQINLVVEDDKVSLEALQEELEGFE 200



 Score = 28.3 bits (60), Expect = 2.9
 Identities = 11/19 (57%), Positives = 16/19 (84%)
 Frame = +1

Query: 727 KIQEFEDFVQSVDIAAFNK 783
           +++ FED+VQS DIAA +K
Sbjct: 195 ELEGFEDYVQSTDIAAMSK 213


>SPBP35G2.10 |mit1||SHREC complex subunit Mit1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1418

 Score = 28.7 bits (61), Expect = 2.2
 Identities = 13/33 (39%), Positives = 21/33 (63%), Gaps = 2/33 (6%)
 Frame = -2

Query: 614 PFQLCALGFPFLSYLSHHPMVL--HQGL*RTLQ 522
           P+QL  L + +L + +HHP +L    GL +T+Q
Sbjct: 558 PYQLKGLNWLYLRWYTHHPCILADEMGLGKTVQ 590


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,246,536
Number of Sequences: 5004
Number of extensions: 71554
Number of successful extensions: 128
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 120
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 127
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 848370472
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)