BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030623sawa_E04_e29_10.seq (1529 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1223.06 |tea1|alp8|cell end marker Tea1|Schizosaccharomyces ... 34 0.060 SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyce... 33 0.14 SPAC1751.01c |gti1||gluconate transporter inducer Gti1|Schizosac... 32 0.18 SPAC32A11.01 |mug8||conserved fungal protein|Schizosaccharomyces... 28 3.0 SPBC3B9.02c |cwf28||splicing factor Cwf28|Schizosaccharomyces po... 28 3.0 SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 28 3.9 SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 28 3.9 SPAC22F8.04 |||triose phosphate transporter |Schizosaccharomyces... 27 5.2 SPCC1450.12 |||conserved fungal protein|Schizosaccharomyces pomb... 27 5.2 SPAC17H9.20 |psc3|SPAC607.01|mitotic cohesin complex, non-SMC su... 27 6.9 SPAC16E8.09 |scd1|ral1|RhoGEF Scd1|Schizosaccharomyces pombe|chr... 27 6.9 SPAC29A4.19c |||P-type ATPase |Schizosaccharomyces pombe|chr 1||... 27 6.9 SPCC1919.07 |||sequence orphan|Schizosaccharomyces pombe|chr 3||... 27 9.1 SPBC216.02 |mcp5|num1, mug21|cortical anchoring factor for dynei... 27 9.1 SPAC6G10.02c |tea3||cell end marker Tea3|Schizosaccharomyces pom... 27 9.1 SPAC10F6.10 |||protein kinase, RIO family |Schizosaccharomyces p... 27 9.1 >SPCC1223.06 |tea1|alp8|cell end marker Tea1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1147 Score = 33.9 bits (74), Expect = 0.060 Identities = 24/83 (28%), Positives = 41/83 (49%), Gaps = 1/83 (1%) Frame = +3 Query: 204 AFQTELRDWTKSKHNEEAVTPNNNTNTLKENADCND-LSLIPKNRNNLLRQLSKALNSLK 380 A +L D T S ++E V N+ + ++EN + L ++ + ++L +QL A S K Sbjct: 652 AKHNKLSDGTFSTPDKENVQSTNDAHIMQENFSLHKALEVMRETSSDLDKQLKDATASQK 711 Query: 381 EDIGDPKAMYKELTFFQNRHKQL 449 E I + KEL + RH + Sbjct: 712 ELIVQTSSFQKELVEERERHNAI 734 >SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyces pombe|chr 1|||Manual Length = 2104 Score = 32.7 bits (71), Expect = 0.14 Identities = 36/139 (25%), Positives = 65/139 (46%) Frame = +3 Query: 336 NNLLRQLSKALNSLKEDIGDPKAMYKELTFFQNRHKQLLNQKTISPKQVSNNRKTINDMF 515 ++L L K L+S+K+D + YKEL + + ++ S + +SN K IN+ Sbjct: 943 SDLSSTLEKKLSSIKKDEQTISSKYKEL---EKDYLNIMADYQHSSQHLSNLEKAINE-- 997 Query: 516 KKLNMVLLGQAGLADGSQHLNELYSFQEKLMNSRQKNIPPEQRKEIGQHYYSIFAAIPRD 695 K LN+ L + + + L + S+ K+ R++N K+ + Y S A+ Sbjct: 998 KNLNIRELNEKLMRLDDELLLKQRSYDTKVQELREEN---ASLKDQCRTYESQLAS---- 1050 Query: 696 DYLELLKKFYQTSTSLKKK 752 L+ K+ +T + L KK Sbjct: 1051 ----LVSKYSETESELNKK 1065 >SPAC1751.01c |gti1||gluconate transporter inducer Gti1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 32.3 bits (70), Expect = 0.18 Identities = 19/66 (28%), Positives = 32/66 (48%) Frame = +3 Query: 465 ISPKQVSNNRKTINDMFKKLNMVLLGQAGLADGSQHLNELYSFQEKLMNSRQKNIPPEQR 644 + PKQ S+N +I+ +++ Q +D +NE YSF + NS KN+ Sbjct: 514 LQPKQPSSNTDSIDQSSYNMSLAASKQFLTSDTQPDVNENYSF---ISNSIPKNLSTSWN 570 Query: 645 KEIGQH 662 + +G H Sbjct: 571 QNMGYH 576 >SPAC32A11.01 |mug8||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 28.3 bits (60), Expect = 3.0 Identities = 19/60 (31%), Positives = 33/60 (55%), Gaps = 4/60 (6%) Frame = +2 Query: 644 KGDRTALLQHIRCYPEG*LFRT-SKEILSDLYIIKEENKKWPPLT---FNAKMSWSKKGK 811 +G+RT LL H+ +T S+E + L +I+E+++ W P T FN + W+ + K Sbjct: 496 EGNRTILL-HLADQMNACSLQTKSRESIKTLKLIEEKDEYWEPETAGYFNTIVGWADQRK 554 >SPBC3B9.02c |cwf28||splicing factor Cwf28|Schizosaccharomyces pombe|chr 2|||Manual Length = 381 Score = 28.3 bits (60), Expect = 3.0 Identities = 19/80 (23%), Positives = 39/80 (48%), Gaps = 3/80 (3%) Frame = +3 Query: 186 KIADQEAFQTELRDWTKSKHNEEAVTPNNNTNTLKENADC--NDLSLIPKNRNNLLRQLS 359 ++A A E+ DW + NE+A N T KE+ D ++ L+ + N+ ++ + Sbjct: 98 RVASSSAADNEIVDWKANNSNEKA---QNKIATNKESTDILPEEVQLVLNDLNDDVKSAN 154 Query: 360 KA-LNSLKEDIGDPKAMYKE 416 A L + ++ + K Y++ Sbjct: 155 SANLQPITTNVVNEKDAYRK 174 >SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces pombe|chr 1|||Manual Length = 1957 Score = 27.9 bits (59), Expect = 3.9 Identities = 39/195 (20%), Positives = 80/195 (41%), Gaps = 4/195 (2%) Frame = +3 Query: 189 IADQEAFQTELRDWTKSKHNEEAVTPNNNTNTLKENADCNDLSLIPKNRNNLLRQLSKAL 368 +AD E Q E DW + + E ++ N L++ A+ + + L + Sbjct: 1322 VADLEKLQHEHDDWLIQRGDLEKALKDSEKNFLRKEAEMTE----------NIHSLEEGK 1371 Query: 369 NSLKEDIGDPKAMYKELTFFQNRHK-QL--LNQKTISPKQVSNNRKT-INDMFKKLNMVL 536 K++I + + ++ N+ K QL LNQ+ + V +++ I + + L+ Sbjct: 1372 EETKKEIAELSSRLEDNQLATNKLKNQLDHLNQEIRLKEDVLKEKESLIISLEESLSNQR 1431 Query: 537 LGQAGLADGSQHLNELYSFQEKLMNSRQKNIPPEQRKEIGQHYYSIFAAIPRDDYLELLK 716 ++ L D L + + +S + I + + + +A+ + L L+ Sbjct: 1432 QKESSLLDAKNELEHMLDDTSRKNSSLMEKI-ESINSSLDDKSFELASAVEK---LGALQ 1487 Query: 717 KFYQTSTSLKKKIKS 761 K + S SL + IKS Sbjct: 1488 KLHSESLSLMENIKS 1502 >SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 673 Score = 27.9 bits (59), Expect = 3.9 Identities = 12/33 (36%), Positives = 19/33 (57%) Frame = +3 Query: 327 KNRNNLLRQLSKALNSLKEDIGDPKAMYKELTF 425 KN+ L +Q S +S+ E + DP+ +Y E F Sbjct: 78 KNKKRLEKQSSAIADSIGESLDDPQTVYDEHLF 110 >SPAC22F8.04 |||triose phosphate transporter |Schizosaccharomyces pombe|chr 1|||Manual Length = 383 Score = 27.5 bits (58), Expect = 5.2 Identities = 15/51 (29%), Positives = 23/51 (45%) Frame = -2 Query: 382 SFKEFKALLNWRSRLFLFLGIRLKSLQSAFSFKVFVLLFGVTASSLCLLFV 230 +F + WR F +RL S F +FV + G+ + + CL FV Sbjct: 126 AFTSLMVKMYWRFSSVHFQTLRLASAIQLKKF-IFVKILGIVSKTYCLAFV 175 >SPCC1450.12 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 821 Score = 27.5 bits (58), Expect = 5.2 Identities = 16/43 (37%), Positives = 24/43 (55%) Frame = +3 Query: 330 NRNNLLRQLSKALNSLKEDIGDPKAMYKELTFFQNRHKQLLNQ 458 N NLL+ LSK+ S+ D D ++K L FQN L+++ Sbjct: 90 NFRNLLKSLSKSNVSVLPDDSDATHLHKWLLKFQNMLTLLMSR 132 >SPAC17H9.20 |psc3|SPAC607.01|mitotic cohesin complex, non-SMC subunit Psc3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 962 Score = 27.1 bits (57), Expect = 6.9 Identities = 21/83 (25%), Positives = 41/83 (49%), Gaps = 7/83 (8%) Frame = +3 Query: 417 LTFFQNRHKQLLNQKTISPKQVSN-------NRKTINDMFKKLNMVLLGQAGLADGSQHL 575 +TF + K+LLN+ I+ KQ+ NR IN++ L ++ Q L + +L Sbjct: 233 MTFLCEKSKELLNEHAIATKQLEKEEKRSRVNRNRINELNNSLGEIVKQQDTL---TTYL 289 Query: 576 NELYSFQEKLMNSRQKNIPPEQR 644 N+ + + + R +++ P+ R Sbjct: 290 NDYF---DSVFVHRYRDVEPKIR 309 >SPAC16E8.09 |scd1|ral1|RhoGEF Scd1|Schizosaccharomyces pombe|chr 1|||Manual Length = 872 Score = 27.1 bits (57), Expect = 6.9 Identities = 9/30 (30%), Positives = 16/30 (53%) Frame = -1 Query: 560 ICKTCLTEEYHIQFFKHVIYCLSVVRHLFR 471 +CK + EYH+ F+ ++ C + L R Sbjct: 441 VCKADIEREYHVYLFEKILLCCKEMSTLKR 470 >SPAC29A4.19c |||P-type ATPase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1096 Score = 27.1 bits (57), Expect = 6.9 Identities = 14/42 (33%), Positives = 23/42 (54%), Gaps = 1/42 (2%) Frame = -1 Query: 299 SVFFQSVCIIIWSNSFFIMFALCPISEFS-LKSLLISNFSFN 177 +V + C +I S+ F LC I +FS + L + N++FN Sbjct: 878 NVLLEGRCSLILSHRCFQYMVLCAIVQFSGVFFLYLKNYNFN 919 >SPCC1919.07 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 206 Score = 26.6 bits (56), Expect = 9.1 Identities = 20/79 (25%), Positives = 37/79 (46%), Gaps = 2/79 (2%) Frame = +3 Query: 63 LQXIRHEGTGXKGRXWLKGIMSKLNIS-VECT-DINSFLSIKGKIADQEAFQTELRDWTK 236 L+ + HE + + R K+NIS E DI+SFL + A + + ++ Sbjct: 114 LESVEHEKSIQEFRKRYNVQKPKVNISHAEAEEDIDSFLESMDQSAPPSITEDKGENYIS 173 Query: 237 SKHNEEAVTPNNNTNTLKE 293 S H+ ++ N++ N + E Sbjct: 174 SNHSSMHISRNDSRNYMSE 192 >SPBC216.02 |mcp5|num1, mug21|cortical anchoring factor for dynein Mcp5/Num1|Schizosaccharomyces pombe|chr 2|||Manual Length = 968 Score = 26.6 bits (56), Expect = 9.1 Identities = 32/133 (24%), Positives = 56/133 (42%), Gaps = 14/133 (10%) Frame = +3 Query: 177 IKGKIADQEAFQTELRDWTKSKHNEEAVTPNNNTNTLKENADCNDLSLIPKNRN------ 338 ++ +A++E TE+ + + A+ NTN E++ + S+ N Sbjct: 74 LEKNLAEEEVRITEM---LTIQRQDIAIERRKNTNCFSESSQVDQYSVTETNLQYATNIG 130 Query: 339 -NLLRQLS--KALNSLKEDIG-----DPKAMYKELTFFQNRHKQLLNQKTISPKQVSNNR 494 NLL+QL + S KED+ D + K+L + K L+ +KT Q Sbjct: 131 LNLLQQLRYLRLQLSEKEDLHKKLSVDNAHLIKQLDLLSSNMKTLMKEKTKVQGQRDLLE 190 Query: 495 KTINDMFKKLNMV 533 + + + KKL V Sbjct: 191 QRLQGLMKKLTEV 203 >SPAC6G10.02c |tea3||cell end marker Tea3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1125 Score = 26.6 bits (56), Expect = 9.1 Identities = 16/66 (24%), Positives = 29/66 (43%) Frame = +3 Query: 504 NDMFKKLNMVLLGQAGLADGSQHLNELYSFQEKLMNSRQKNIPPEQRKEIGQHYYSIFAA 683 ND+ K+L+ V+ Q S +++ Y +N + E+ + I +YY+ Sbjct: 1038 NDLHKRLSDVINSQQKFLSLSPQVSKDYYDVRSKLNDTAGSFSGEEMRAIDDNYYASRIK 1097 Query: 684 IPRDDY 701 DDY Sbjct: 1098 QLEDDY 1103 >SPAC10F6.10 |||protein kinase, RIO family |Schizosaccharomyces pombe|chr 1|||Manual Length = 521 Score = 26.6 bits (56), Expect = 9.1 Identities = 13/51 (25%), Positives = 26/51 (50%) Frame = +3 Query: 192 ADQEAFQTELRDWTKSKHNEEAVTPNNNTNTLKENADCNDLSLIPKNRNNL 344 +D + F+ + DW ++ N A N N+ K N+ + +S + + NN+ Sbjct: 25 SDNDYFREDDIDWNQASSNYSARQENFGNNSSKINSVNDHVSTLSRYVNNI 75 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 5,060,258 Number of Sequences: 5004 Number of extensions: 99708 Number of successful extensions: 308 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 288 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 308 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 858281342 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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