Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030623sawa_E04_e29_10.seq
(1529 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g45750.1 68416.m04944 expressed protein 33 0.50
At1g23570.1 68414.m02966 expressed protein contains Pfam profile... 33 0.66
At1g72390.1 68414.m08373 expressed protein 31 2.0
At3g11850.2 68416.m01453 expressed protein contains Pfam profile... 30 3.5
At3g11850.1 68416.m01452 expressed protein contains Pfam profile... 30 3.5
At1g69120.1 68414.m07909 floral homeotic protein APETALA1 (AP1) ... 30 3.5
At2g26340.1 68415.m03160 expressed protein 30 4.6
At2g19060.1 68415.m02226 GDSL-motif lipase/hydrolase family prot... 30 4.6
At1g31810.1 68414.m03904 formin homology 2 domain-containing pro... 30 4.6
At5g41810.1 68418.m05090 expressed protein 29 6.1
At3g61780.1 68416.m06931 expressed protein ; expression supporte... 29 6.1
>At3g45750.1 68416.m04944 expressed protein
Length = 682
Score = 33.1 bits (72), Expect = 0.50
Identities = 27/101 (26%), Positives = 50/101 (49%)
Frame = +3
Query: 444 QLLNQKTISPKQVSNNRKTINDMFKKLNMVLLGQAGLADGSQHLNELYSFQEKLMNSRQK 623
++LN S + VS + T ++ K LN + L G ++ S + E Y M S Q
Sbjct: 45 KVLNDVYCSFRPVSADYNTRKELVKNLNTMALDIYGKSEESSPVLEAYGSFVMDMYSSQS 104
Query: 624 NIPPEQRKEIGQHYYSIFAAIPRDDYLELLKKFYQTSTSLK 746
++ ++ ++ + + IPR+ LE+LK+F + SL+
Sbjct: 105 DL------DVSINFGNGTSEIPREKKLEILKRFAKKLRSLQ 139
>At1g23570.1 68414.m02966 expressed protein contains Pfam profile
PF02713: Domain of unknown function DUF220
Length = 314
Score = 32.7 bits (71), Expect = 0.66
Identities = 17/56 (30%), Positives = 29/56 (51%)
Frame = +3
Query: 480 VSNNRKTINDMFKKLNMVLLGQAGLADGSQHLNELYSFQEKLMNSRQKNIPPEQRK 647
V NRK + +KK M+L+ + +GS + LY E+L N+ + P E ++
Sbjct: 170 VDENRKDLTAKYKKKKMILM---KVFEGSYRVEPLYVDSERLCNNMEPKSPAEYKR 222
>At1g72390.1 68414.m08373 expressed protein
Length = 1088
Score = 31.1 bits (67), Expect = 2.0
Identities = 30/133 (22%), Positives = 59/133 (44%), Gaps = 4/133 (3%)
Frame = +3
Query: 237 SKHNEEAVTPNNNTNTLKENADCNDLSLIPKNRNNLLRQLSKALNSLKEDIGDPKAMYKE 416
SKH TPNN+T + NA ++P + L Q+S+ L S P+ + +
Sbjct: 624 SKHGNTGNTPNNSTQNILANA-----RMVPPTNSQAL-QMSQGLLSGVSMPMQPQQLDPQ 677
Query: 417 LTFFQNRHKQLLNQKTISPKQ--VSNNRKTINDMFKKLNMV--LLGQAGLADGSQHLNEL 584
+ + H Q NQ+++ +Q R ++ L+ + + +G+ G Q N+
Sbjct: 678 QSALLSSHSQQKNQQSMFTQQQHPQMQRPSMILPTNPLSAINSMSQSSGMQPGGQMANKY 737
Query: 585 YSFQEKLMNSRQK 623
Q +++ +Q+
Sbjct: 738 SPLQLQMLQQQQQ 750
>At3g11850.2 68416.m01453 expressed protein contains Pfam profile
PF04576: Protein of unknown function, DUF593
Length = 504
Score = 30.3 bits (65), Expect = 3.5
Identities = 14/49 (28%), Positives = 26/49 (53%)
Frame = +3
Query: 414 ELTFFQNRHKQLLNQKTISPKQVSNNRKTINDMFKKLNMVLLGQAGLAD 560
+L F N Q+ N+ + + VSN ++TI D++ +L + + AD
Sbjct: 47 DLDMFSNPRVQIENECELLRETVSNQQQTIQDLYHELEQERIASSTAAD 95
>At3g11850.1 68416.m01452 expressed protein contains Pfam profile
PF04576: Protein of unknown function, DUF593
Length = 504
Score = 30.3 bits (65), Expect = 3.5
Identities = 14/49 (28%), Positives = 26/49 (53%)
Frame = +3
Query: 414 ELTFFQNRHKQLLNQKTISPKQVSNNRKTINDMFKKLNMVLLGQAGLAD 560
+L F N Q+ N+ + + VSN ++TI D++ +L + + AD
Sbjct: 47 DLDMFSNPRVQIENECELLRETVSNQQQTIQDLYHELEQERIASSTAAD 95
>At1g69120.1 68414.m07909 floral homeotic protein APETALA1 (AP1) /
agamous-like MADS box protein (AGL7) identical to
SP|P35631 Floral homeotic protein APETALA1 (AGL7
protein) {Arabidopsis thaliana}
Length = 256
Score = 30.3 bits (65), Expect = 3.5
Identities = 16/63 (25%), Positives = 30/63 (47%)
Frame = +3
Query: 315 SLIPKNRNNLLRQLSKALNSLKEDIGDPKAMYKELTFFQNRHKQLLNQKTISPKQVSNNR 494
++ PK NL +QL AL ++ + MY+ + Q + K + Q ++ KQ+
Sbjct: 119 AMSPKELQNLEQQLDTALKHIRTRKN--QLMYESINELQKKEKAIQEQNSMLSKQIKERE 176
Query: 495 KTI 503
K +
Sbjct: 177 KIL 179
>At2g26340.1 68415.m03160 expressed protein
Length = 230
Score = 29.9 bits (64), Expect = 4.6
Identities = 19/71 (26%), Positives = 34/71 (47%)
Frame = +3
Query: 423 FFQNRHKQLLNQKTISPKQVSNNRKTINDMFKKLNMVLLGQAGLADGSQHLNELYSFQEK 602
F + HK+L T+SP +R+ I+ F ++LL +A G + Y ++K
Sbjct: 13 FSNSNHKKLTCAATLSPPPWKQSRRVISVSFFLSRLLLLPNDAMAGGLMDKLDKYVKRKK 72
Query: 603 LMNSRQKNIPP 635
L + + +PP
Sbjct: 73 L-DPLEAYVPP 82
>At2g19060.1 68415.m02226 GDSL-motif lipase/hydrolase family protein
similar to family II lipases EXL6 GI:15054390, EXL1
GI:15054382, EXL2 GI:15054384 from [Arabidopsis
thaliana]; contains Pfam profile PF00657: GDSL-like
Lipase/Acylhydrolase
Length = 349
Score = 29.9 bits (64), Expect = 4.6
Identities = 17/42 (40%), Positives = 26/42 (61%), Gaps = 5/42 (11%)
Frame = +3
Query: 540 GQAGLADG-SQHLNELYSFQEKLMNSRQ----KNIPPEQRKE 650
G AGL + SQHL E SF++++ N R+ +PPE+ K+
Sbjct: 110 GGAGLLEETSQHLGERISFEKQITNHRKMIMTAGVPPEKLKK 151
>At1g31810.1 68414.m03904 formin homology 2 domain-containing protein
/ FH2 domain-containing protein low similarity to
SP|P48608 Diaphanous protein {Drosophila melanogaster};
contains Pfam profile PF02181: Formin Homology 2(FH2)
Domain
Length = 1201
Score = 29.9 bits (64), Expect = 4.6
Identities = 16/45 (35%), Positives = 18/45 (40%)
Frame = -1
Query: 1268 PAPGFFXXPXRFYSAXKXPXPTPXSRXPIPXGGETQKTXMVPPPP 1134
PAP A P P P S+ P P KT + PPPP
Sbjct: 699 PAPPPLPPSSTRLGAPPPPPPPPLSKTPAPPPPPLSKTPVPPPPP 743
>At5g41810.1 68418.m05090 expressed protein
Length = 288
Score = 29.5 bits (63), Expect = 6.1
Identities = 19/77 (24%), Positives = 35/77 (45%), Gaps = 6/77 (7%)
Frame = +3
Query: 162 NSFLSIKGKIADQEAFQTELRDWTKSKHNEEAVTPNNNTNTLKENA------DCNDLSLI 323
+S L +K K+ + F ++ + TK + ++ T NNN + +K DC
Sbjct: 72 SSSLEMKKKLEIDQKFTSQNEENTKKEEEKKKETNNNNLSNMKHKKTSSHVWDCGSTLYD 131
Query: 324 PKNRNNLLRQLSKALNS 374
N+ RQL A+++
Sbjct: 132 SFELNSFKRQLDSAISA 148
>At3g61780.1 68416.m06931 expressed protein ; expression supported
by MPSS
Length = 1121
Score = 29.5 bits (63), Expect = 6.1
Identities = 12/36 (33%), Positives = 21/36 (58%)
Frame = +3
Query: 150 CTDINSFLSIKGKIADQEAFQTELRDWTKSKHNEEA 257
C D++S +K ++A EL DW +++N+EA
Sbjct: 95 CDDLSSTEGLKDRVAQDSNLLNELEDWV-ARYNKEA 129
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 27,623,352
Number of Sequences: 28952
Number of extensions: 577819
Number of successful extensions: 1494
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 1410
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1488
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4096401600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -