Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030623sawa_D11_e84_07.seq
(1507 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 279 9e-76
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 279 9e-76
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 279 9e-76
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 87 4e-18
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 50 6e-07
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 40 9e-04
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 27 5.1
SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyc... 27 8.9
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 279 bits (683), Expect = 9e-76
Identities = 126/167 (75%), Positives = 145/167 (86%)
Frame = -1
Query: 883 GNIPVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPP 704
G I FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP
Sbjct: 273 GMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPP 332
Query: 703 RGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSI 524
G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E++PK +
Sbjct: 333 MGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFV 392
Query: 523 KSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 383
KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 393 KSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 279 bits (683), Expect = 9e-76
Identities = 126/167 (75%), Positives = 145/167 (86%)
Frame = -1
Query: 883 GNIPVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPP 704
G I FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP
Sbjct: 273 GMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPP 332
Query: 703 RGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSI 524
G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E++PK +
Sbjct: 333 MGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFV 392
Query: 523 KSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 383
KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 393 KSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 279 bits (683), Expect = 9e-76
Identities = 126/167 (75%), Positives = 145/167 (86%)
Frame = -1
Query: 883 GNIPVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPP 704
G I FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP
Sbjct: 273 GMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPP 332
Query: 703 RGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSI 524
G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E++PK +
Sbjct: 333 MGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFV 392
Query: 523 KSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 383
KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 393 KSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 87.4 bits (207), Expect = 4e-18
Identities = 52/163 (31%), Positives = 86/163 (52%), Gaps = 2/163 (1%)
Frame = -1
Query: 862 PANITTEVKSV-EMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADF 686
P N T EV ++ + E + ++ GD V V+ +++ GYV +KN P F
Sbjct: 503 PINQTLEVTAIYDEADEEISSSICGDQVRLRVRGDD-SDVQTGYVLTSTKN-PVHATTRF 560
Query: 685 TAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAA 506
AQ+ +L P ++ GY+ V+ HTA FA++ K+D+ T + ++ P G
Sbjct: 561 IAQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHKLDK-TNRKSKKPPMFATKGMKI 619
Query: 505 IVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVG-VIKAVN 380
I L P+C+E F+++ +GRF +RD TVAVG V+K ++
Sbjct: 620 IAELETQTPVCMERFEDYQYMGRFTLRDQGTTVAVGKVVKILD 662
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 50.4 bits (115), Expect = 6e-07
Identities = 42/138 (30%), Positives = 63/138 (45%)
Frame = -1
Query: 802 AVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPVL 623
AV GD V + ++ V +LR G + + +N P R F A++ + G I +G T VL
Sbjct: 461 AVAGDTVTLQLADIEVNQLRPGDILSNYEN-PVRRVRSFVAEIQTFDIHGPILSGSTLVL 519
Query: 622 DCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 443
H+ + K+ K + + S K I L PLC+ +E P L
Sbjct: 520 -----HLGRTVTSVSLKIVTVNNKRSR-HIASRKRALVRISFLDGLFPLCLA--EECPAL 571
Query: 442 GRFAVRDMRQTVAVGVIK 389
GRF +R TVA G++K
Sbjct: 572 GRFILRRSGDTVAAGIVK 589
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 39.9 bits (89), Expect = 9e-04
Identities = 34/156 (21%), Positives = 66/156 (42%)
Frame = -1
Query: 859 ANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTA 680
+++ T V +EM + L AV GDN G ++++ ++L+RG + P F A
Sbjct: 292 SHLKTTVTGIEMFKKQLDAAVAGDNCGLLLRSIKREQLKRGMIVAQPGTVAPH--QKFKA 349
Query: 679 QVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIV 500
+L + T +D + + + +++ ++ T D+ K + GD +
Sbjct: 350 SFYILTK--EEGGRRTGFVDKYRPQLYSRTSDVTVEL---THPDPNDSDKMVMPGDNVEM 404
Query: 499 NLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVI 392
P+ +E Q RF VR+ TV ++
Sbjct: 405 ICTLIHPIVIEKGQ------RFTVREGGSTVGTALV 434
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 27.5 bits (58), Expect = 5.1
Identities = 12/25 (48%), Positives = 19/25 (76%)
Frame = +3
Query: 504 MAASPDLMDLGLSSVDLPVRRSTFS 578
++ +PDL D+ LSSVD P++ +T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
>SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1190
Score = 26.6 bits (56), Expect = 8.9
Identities = 19/71 (26%), Positives = 34/71 (47%), Gaps = 3/71 (4%)
Frame = -2
Query: 531 NPLNLVMPPLSTWFPPSPCVWS---PSRNSHPSVVSPCVT*GRRSPSV**RL*TSRKQVV 361
+PL+ + P L WF V+S P+R+S + P + + SP+ +++V+
Sbjct: 707 SPLSELHPQLREWFSSKQSVYSLQYPTRDSIIAFFQPILELIKASPTELPGGIPRKRRVL 766
Query: 360 AKSPKLPKKPP 328
+ P P PP
Sbjct: 767 PELPLAPDPPP 777
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,888,043
Number of Sequences: 5004
Number of extensions: 94760
Number of successful extensions: 244
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 234
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 243
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 842423950
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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