Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030623sawa_D11_e84_07.seq
(1507 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 262 3e-70
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 262 3e-70
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 262 3e-70
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 262 3e-70
At1g35550.1 68414.m04414 elongation factor Tu C-terminal domain-... 141 9e-34
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 83 6e-16
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 63 4e-10
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 45 1e-04
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 40 0.006
At4g27900.2 68417.m04005 expressed protein 32 1.1
At4g27900.1 68417.m04004 expressed protein 32 1.1
At1g45160.1 68414.m05177 protein kinase family protein contains ... 31 2.6
At1g28280.1 68414.m03471 VQ motif-containing protein contains PF... 29 6.0
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 262 bits (643), Expect = 3e-70
Identities = 116/168 (69%), Positives = 145/168 (86%)
Frame = -1
Query: 883 GNIPVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPP 704
G + FAP +TTEVKSVEMHHE+L EA+PGDNVGFNVKNV+VK+L+RGYVA +SK++P
Sbjct: 263 GMVVTFAPTGLTTEVKSVEMHHESLLEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPA 322
Query: 703 RGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSI 524
+GAA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KF+EI K+DRR+GK E PK +
Sbjct: 323 KGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDRRSGKEIEKEPKFL 382
Query: 523 KSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVN 380
K+GDA +V + P+KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V+
Sbjct: 383 KNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVD 430
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 262 bits (643), Expect = 3e-70
Identities = 116/168 (69%), Positives = 145/168 (86%)
Frame = -1
Query: 883 GNIPVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPP 704
G + FAP +TTEVKSVEMHHE+L EA+PGDNVGFNVKNV+VK+L+RGYVA +SK++P
Sbjct: 263 GMVVTFAPTGLTTEVKSVEMHHESLLEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPA 322
Query: 703 RGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSI 524
+GAA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KF+EI K+DRR+GK E PK +
Sbjct: 323 KGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDRRSGKEIEKEPKFL 382
Query: 523 KSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVN 380
K+GDA +V + P+KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V+
Sbjct: 383 KNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVD 430
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 262 bits (643), Expect = 3e-70
Identities = 116/168 (69%), Positives = 145/168 (86%)
Frame = -1
Query: 883 GNIPVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPP 704
G + FAP +TTEVKSVEMHHE+L EA+PGDNVGFNVKNV+VK+L+RGYVA +SK++P
Sbjct: 263 GMVVTFAPTGLTTEVKSVEMHHESLLEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPA 322
Query: 703 RGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSI 524
+GAA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KF+EI K+DRR+GK E PK +
Sbjct: 323 KGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDRRSGKEIEKEPKFL 382
Query: 523 KSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVN 380
K+GDA +V + P+KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V+
Sbjct: 383 KNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVD 430
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 262 bits (643), Expect = 3e-70
Identities = 116/168 (69%), Positives = 145/168 (86%)
Frame = -1
Query: 883 GNIPVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPP 704
G + FAP +TTEVKSVEMHHE+L EA+PGDNVGFNVKNV+VK+L+RGYVA +SK++P
Sbjct: 263 GMVVTFAPTGLTTEVKSVEMHHESLLEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPA 322
Query: 703 RGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSI 524
+GAA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KF+EI K+DRR+GK E PK +
Sbjct: 323 KGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDRRSGKEIEKEPKFL 382
Query: 523 KSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVN 380
K+GDA +V + P+KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V+
Sbjct: 383 KNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVD 430
>At1g35550.1 68414.m04414 elongation factor Tu C-terminal
domain-containing protein similar to SP|P13905
Elongation factor 1-alpha (EF-1-alpha) {Arabidopsis
thaliana}; contains Pfam profile PF03143: Elongation
factor Tu C-terminal domain
Length = 104
Score = 141 bits (342), Expect = 9e-34
Identities = 61/95 (64%), Positives = 77/95 (81%)
Frame = -1
Query: 667 LNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVP 488
+NH GQI NGYTPVLDCHT+HIA KF+EI K+D RTG E PK +K+ +AAI+N+ P
Sbjct: 1 MNHLGQIKNGYTPVLDCHTSHIAVKFSEILTKIDWRTGHEIEKEPKFLKNSEAAIINMTP 60
Query: 487 SKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 383
+KP+ VE++ +PPLGRFA+RDMRQTV VGVIK+V
Sbjct: 61 TKPMVVEAYSAYPPLGRFAIRDMRQTVGVGVIKSV 95
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 82.6 bits (195), Expect = 6e-16
Identities = 44/166 (26%), Positives = 82/166 (49%), Gaps = 2/166 (1%)
Frame = -1
Query: 883 GNIPVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPP 704
G+ V P +V ++ + ++ A PG+N+ + + +++ G+V S NP
Sbjct: 358 GDSLVVMPNKEQVKVVAIYCDEDKVKRAGPGENLRVRITGIEDEDILSGFVLS-SIVNPV 416
Query: 703 RGAADFTAQVIVLN--HPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPK 530
+F AQ+ +L + GY +L H C+ E+K ++D +T K +
Sbjct: 417 PAVTEFVAQLQILELLDNAIFTAGYKAILHIHAVVEECEIIELKSQIDLKTRKPMKKKVL 476
Query: 529 SIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVI 392
+K+G A + + + +C+E F +FP LGRF +R +T+AVG +
Sbjct: 477 FVKNGAAVVCRIQVTNSICIEKFSDFPQLGRFTLRTEGKTIAVGKV 522
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 63.3 bits (147), Expect = 4e-10
Identities = 42/167 (25%), Positives = 77/167 (46%)
Frame = -1
Query: 883 GNIPVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPP 704
G+ + P+ ++S+E +A A GDNV ++ + ++ G V + P
Sbjct: 498 GSKVMVMPSGDQGTIRSLERDSQACTIARAGDNVALALQGIDANQVMAGDVLCHP-DFPV 556
Query: 703 RGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSI 524
A V+VL I G H A A ++ +D +TG+ T+ +P+ +
Sbjct: 557 SVATHLELMVLVLEGATPILLGSQLEFHVHHAKEAATVVKLVAMLDPKTGQPTKKSPRCL 616
Query: 523 KSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 383
+ +A++ + P+CVE+F E LGR +R +TVA+G + +
Sbjct: 617 TAKQSAMLEVSLQNPVCVETFSESRALGRVFLRSSGRTVAMGKVTRI 663
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 45.2 bits (102), Expect = 1e-04
Identities = 38/153 (24%), Positives = 66/153 (43%)
Frame = -1
Query: 841 VKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLN 662
V VEM + L EA+ GDNVG ++ + +++RG V + P F A + VL
Sbjct: 333 VTGVEMFQKILDEALAGDNVGLLLRGIQKADIQRGMVLAKPGSITPH--TKFEAIIYVLK 390
Query: 661 HPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSK 482
+ ++P + + ++ KV + E++ K + GD + +
Sbjct: 391 K--EEGGRHSPFFAGYRPQFYMRTTDVTGKVTKIMNDKDEES-KMVMPGDRVKIVVELIV 447
Query: 481 PLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 383
P+ E RFA+R+ +TV GVI +
Sbjct: 448 PVACEQGM------RFAIREGGKTVGAGVIGTI 474
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 39.5 bits (88), Expect = 0.006
Identities = 42/159 (26%), Positives = 70/159 (44%), Gaps = 2/159 (1%)
Frame = -1
Query: 853 ITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQV 674
+ + V VEM + L GDNVG ++ + ++++RG V +K + F A++
Sbjct: 312 LKSTVTGVEMFKKILDNGQAGDNVGLLLRGLKREDIQRGMVI--AKPGSCKTYKKFEAEI 369
Query: 673 IVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGD--AAIV 500
VL +T + + A+I KV+ +N K + GD A+
Sbjct: 370 YVLTK--DEGGRHTAFFSNYRPQFYLRTADITGKVE------LPENVKMVMPGDNVTAVF 421
Query: 499 NLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 383
L+ PL E+ Q RFA+R+ +TV GV+ V
Sbjct: 422 ELIMPVPL--ETGQ------RFALREGGRTVGAGVVSKV 452
>At4g27900.2 68417.m04005 expressed protein
Length = 261
Score = 31.9 bits (69), Expect = 1.1
Identities = 11/31 (35%), Positives = 21/31 (67%)
Frame = -1
Query: 535 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 443
PKS+ SG+ + +++V K ++ F +FPP+
Sbjct: 144 PKSVSSGNLSSMDMVEHKDAVIQGFPDFPPV 174
>At4g27900.1 68417.m04004 expressed protein
Length = 261
Score = 31.9 bits (69), Expect = 1.1
Identities = 11/31 (35%), Positives = 21/31 (67%)
Frame = -1
Query: 535 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 443
PKS+ SG+ + +++V K ++ F +FPP+
Sbjct: 144 PKSVSSGNLSSMDMVEHKDAVIQGFPDFPPV 174
>At1g45160.1 68414.m05177 protein kinase family protein contains
eukaryotic protein kinase domain, INTERPRO:IPR000719
Length = 1067
Score = 30.7 bits (66), Expect = 2.6
Identities = 21/67 (31%), Positives = 28/67 (41%), Gaps = 1/67 (1%)
Frame = -1
Query: 637 YTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLCVESF- 461
Y P CH AC E++ R S +D K IK + N SK + ++SF
Sbjct: 82 YKPKYSCHPPKYACSSTTSSEEIKLRGKNSGKDEEKMIKISE---TNPPCSKSMGIKSFS 138
Query: 460 QEFPPLG 440
E P G
Sbjct: 139 HELGPRG 145
>At1g28280.1 68414.m03471 VQ motif-containing protein contains
PF05678: VQ motif
Length = 247
Score = 29.5 bits (63), Expect = 6.0
Identities = 17/39 (43%), Positives = 23/39 (58%), Gaps = 1/39 (2%)
Frame = -2
Query: 534 LNPLNLVMPPLSTWFPP-SPCVWSPSRNSHPSVVSPCVT 421
+NPLN V P+++ F P P + SPS PS+V VT
Sbjct: 140 INPLNPVFNPVNSAFSPRKPEILSPSILDFPSLVLSPVT 178
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 26,804,894
Number of Sequences: 28952
Number of extensions: 537201
Number of successful extensions: 1444
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 1347
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1438
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4019292864
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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