BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030623sawa_D01_e4_07.seq (1564 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g41140.1 68418.m05001 expressed protein 35 0.13 At5g52280.1 68418.m06488 protein transport protein-related low s... 35 0.17 At2g25350.1 68415.m03032 phox (PX) domain-containing protein wea... 34 0.29 At2g27170.1 68415.m06029 structural maintenance of chromosomes (... 33 0.68 At1g01660.1 68414.m00084 U-box domain-containing protein 32 1.2 At3g53350.1 68416.m05886 myosin heavy chain-related low similari... 31 2.7 At1g74830.1 68414.m08670 expressed protein contains Pfam profile... 31 2.7 At3g53350.3 68416.m05888 myosin heavy chain-related low similari... 30 3.6 At3g53350.2 68416.m05887 myosin heavy chain-related low similari... 30 3.6 At4g02660.1 68417.m00361 WD-40 repeat family protein / beige-rel... 30 4.8 At1g20960.1 68414.m02624 U5 small nuclear ribonucleoprotein heli... 30 4.8 At1g04600.1 68414.m00454 myosin, putative similar to myosin (GI:... 30 4.8 At4g18200.1 68417.m02705 purine permease family protein similar ... 29 6.3 At3g05130.1 68416.m00557 expressed protein ; expression supporte... 29 6.3 At4g14760.1 68417.m02271 M protein repeat-containing protein con... 29 8.3 At3g45280.1 68416.m04889 syntaxin 72 (SYP72) identical to syntax... 29 8.3 At3g44050.1 68416.m04718 kinesin motor protein-related KLP2 prot... 29 8.3 >At5g41140.1 68418.m05001 expressed protein Length = 983 Score = 35.1 bits (77), Expect = 0.13 Identities = 36/170 (21%), Positives = 68/170 (40%), Gaps = 2/170 (1%) Frame = +1 Query: 97 KDALQNLNDRLAAYIDKVRQLESENSGLRREIQTTQEVVTREVSNIKGIYEHELQDARKL 276 ++ L N ND L +V ++E E E+ ++ T+E+ + E++ + + Sbjct: 673 EELLMNANDEL-----RVNRVEYEAK--LNELSGKTDLKTKEMKRMSADLEYQKRQKEDV 725 Query: 277 LDDTSRE--KAKLEIDLKRLYXENXXXXXXXXXXXXXCQQAENLARHYETRFTEESNKYN 450 D + E + K EI++ RL E ++ + + E T ++ Sbjct: 726 NADLTHEITRRKDEIEILRLDLEETRKSSMETEASLS-EELQRIIDEKEAVITALKSQLE 784 Query: 451 TALADKKKAQDEARDLAKELEKLRKVYADTRXTLEEEMLCRIXMENTVXS 600 TA+A + + E+E LRK R LE++ +EN S Sbjct: 785 TAIAPCDNLKHSLSNNESEIENLRKQVVQVRSELEKKEEEMANLENREAS 834 >At5g52280.1 68418.m06488 protein transport protein-related low similarity to SP|P25386 Intracellular protein transport protein USO1 {Saccharomyces cerevisiae} Length = 853 Score = 34.7 bits (76), Expect = 0.17 Identities = 40/163 (24%), Positives = 71/163 (43%), Gaps = 3/163 (1%) Frame = +1 Query: 79 TAVFK*KDALQNLNDRLAAYIDKVRQLESENSGLRRE-IQTTQEV--VTREVSNIKGIYE 249 ++V + D ++ L L A + E E LR++ I+ ++ + +++EVS +KG + Sbjct: 257 SSVTESSDPIERLKMELEALRRQSELSELEKQSLRKQAIKESKRIQELSKEVSCLKGERD 316 Query: 250 HELQDARKLLDDTSREKAKLEIDLKRLYXENXXXXXXXXXXXXXCQQAENLARHYETRFT 429 +++ KL SR++A E L R E+ C++ + + T Sbjct: 317 GAMEECEKLRLQNSRDEADAESRL-RCISEDSSNMIEEIRDELSCEKDLTSNLKLQLQRT 375 Query: 430 EESNKYNTALADKKKAQDEARDLAKELEKLRKVYADTRXTLEE 558 +ESN N LA RDL + LE+ + LEE Sbjct: 376 QESNS-NLILA--------VRDLNEMLEQKNNEISSLNSLLEE 409 >At2g25350.1 68415.m03032 phox (PX) domain-containing protein weak similarity to SP|Q9UTK5 Abnormal long morphology protein 1 (Sp8) {Schizosaccharomyces pombe}; contains Pfam profile PF00787: PX domain Length = 643 Score = 33.9 bits (74), Expect = 0.29 Identities = 42/183 (22%), Positives = 75/183 (40%), Gaps = 1/183 (0%) Frame = +1 Query: 61 ARVLQDTAVFK*KDALQNLNDRLAAYIDKVRQLESENSGLRREIQTTQEVVTREVSNIKG 240 AR+ Q+TAV K+ L D L ++ +Q EN L + + T ++ VT+ +++ Sbjct: 404 ARLNQETAV---KEYLNRKVDDLEVELETTKQRNKEN--LEQALMTERQSVTKMQWDMEE 458 Query: 241 IYEHELQDARKLLDDTSREKAKLEIDLKRLYXENXXXXXXXXXXXXXC-QQAENLARHYE 417 + + + KL + K D K QQ E+L+R Y Sbjct: 459 LRQKTFEMELKL-----KSKEDGSSDSKTSGNSTISESHELLQEMDATKQQLEDLSRRYV 513 Query: 418 TRFTEESNKYNTALADKKKAQDEARDLAKELEKLRKVYADTRXTLEEEMLCRIXMENTVX 597 + + + K + ++ KEL + +DT L++E RI +ENT+ Sbjct: 514 ELEAKSKADIKVLVREVKSLRRSHMEMEKELTRSLTEKSDTEKLLQQE---RIIVENTLE 570 Query: 598 SLR 606 + R Sbjct: 571 ARR 573 >At2g27170.1 68415.m06029 structural maintenance of chromosomes (SMC) family protein similar to basement membrane-associated chondroitin proteoglycan Bamacan [Rattus norvegicus] GI:1785540; contains Pfam profile PF02463: RecF/RecN/SMC N terminal domain. No suitalble start codon was identified. Length = 1207 Score = 32.7 bits (71), Expect = 0.68 Identities = 28/122 (22%), Positives = 55/122 (45%), Gaps = 2/122 (1%) Frame = +1 Query: 136 YID-KVRQLESENSGLRREIQTTQEVVTREVSNIKGIYEHELQDARKLLDDTSREKAKLE 312 Y+D ++R+L+ E LR+ Q ++ + E + IY+ EL DAR+ L+ + K Sbjct: 195 YLDERLRELDEEKEELRKYQQLDKQRKSLEYT----IYDKELHDAREKLEQVEVARTKAS 250 Query: 313 IDLKRLYXENXXXXXXXXXXXXXCQQ-AENLARHYETRFTEESNKYNTALADKKKAQDEA 489 + ++Y ++ + L Y+ + T E+ + AL K K + + Sbjct: 251 EESTKMYDRVEKAQDDSKSLDESLKELTKELQTLYKEKETVEAQQ-TKALKKKTKLELDV 309 Query: 490 RD 495 +D Sbjct: 310 KD 311 >At1g01660.1 68414.m00084 U-box domain-containing protein Length = 568 Score = 31.9 bits (69), Expect = 1.2 Identities = 39/187 (20%), Positives = 78/187 (41%), Gaps = 15/187 (8%) Frame = +1 Query: 97 KDALQNLND-RLAAYIDKVRQLESENSGLRREIQTTQ-EVVTREVSNIKGIYEHELQDAR 270 K AL + + + AY + VR+ ++EN+ + + E + E + ++ + L R Sbjct: 231 KQALMEVEESKREAYEECVRRFKAENTAVEAIRSAREYEAMYNEEAKLRKEGKEALAKQR 290 Query: 271 KLLDDTSREKAKLEIDL---KRLYXENXXXXXXXXXXXXX-CQQAENLARHYET--RFTE 432 K+++ T +E+ I + ++LY E ++ E + E + Sbjct: 291 KMVEKTKQERDDALIIILNGRKLYNEELRRRVEAEEMLGKEKEEHERTKKEIEEVRAIVQ 350 Query: 433 ESNKYNTALADKKKAQDEARDLAKELEKLRK-------VYADTRXTLEEEMLCRIXMENT 591 + YN L +K+ ++ + +ELEK +K + + E+E+ R E Sbjct: 351 DGTLYNEQLRHRKEMEESMKRQEEELEKTKKEKEEACMISKNLMQLYEDEVRQRKEAEEL 410 Query: 592 VXSLREE 612 V REE Sbjct: 411 VKRRREE 417 >At3g53350.1 68416.m05886 myosin heavy chain-related low similarity to filamin-interacting protein S-FILIP [Rattus norvegicus] GI:21392397, nonmuscle heavy chain myosin II-A [Mus musculus] GI:17978023 Length = 396 Score = 30.7 bits (66), Expect = 2.7 Identities = 30/144 (20%), Positives = 66/144 (45%), Gaps = 1/144 (0%) Frame = +1 Query: 115 LNDRLAAYIDKVRQLESENSGLRREIQTTQEVVTREVSNIKGIYEHELQDAR-KLLDDTS 291 LN+ ++ +LES S L+ E++ +E + R + +K + E +DA+ +L+D + Sbjct: 62 LNEIQKKRTGRIPELESTISQLQEELKKAKEELNRSEA-LKREAQEEAEDAKHQLMDINA 120 Query: 292 REKAKLEIDLKRLYXENXXXXXXXXXXXXXCQQAENLARHYETRFTEESNKYNTALADKK 471 E +++E +L++L E Q+ + + E K + L + + Sbjct: 121 SEDSRIE-ELRKLSQER---DKTWQSELEAMQRQHGMDSTALSSAINEVQKLKSKLFESE 176 Query: 472 KAQDEARDLAKELEKLRKVYADTR 543 ++++ + LEKL + + R Sbjct: 177 SELEQSKYEVRSLEKLVRQLEEER 200 >At1g74830.1 68414.m08670 expressed protein contains Pfam profile PF04576: Protein of unknown function, DUF593 Length = 542 Score = 30.7 bits (66), Expect = 2.7 Identities = 12/45 (26%), Positives = 26/45 (57%) Frame = +1 Query: 385 QQAENLARHYETRFTEESNKYNTALADKKKAQDEARDLAKELEKL 519 + EN+ ++ +++ +EES N AD++K + + KEL ++ Sbjct: 445 ENGENIDQNGQSKRSEESTAENVVSADEEKGSESKEGIVKELSEI 489 >At3g53350.3 68416.m05888 myosin heavy chain-related low similarity to filamin-interacting protein S-FILIP [Rattus norvegicus] GI:21392397, nonmuscle heavy chain myosin II-A [Mus musculus] GI:17978023 Length = 394 Score = 30.3 bits (65), Expect = 3.6 Identities = 28/134 (20%), Positives = 63/134 (47%), Gaps = 1/134 (0%) Frame = +1 Query: 145 KVRQLESENSGLRREIQTTQEVVTREVSNIKGIYEHELQDAR-KLLDDTSREKAKLEIDL 321 ++ +LES S L+ E++ +E + R + +K + E +DA+ +L+D + E +++E +L Sbjct: 70 RIPELESTISQLQEELKKAKEELNRSEA-LKREAQEEAEDAKHQLMDINASEDSRIE-EL 127 Query: 322 KRLYXENXXXXXXXXXXXXXCQQAENLARHYETRFTEESNKYNTALADKKKAQDEARDLA 501 ++L E Q+ + + E K + L + + ++++ Sbjct: 128 RKLSQER---DKTWQSELEAMQRQHGMDSTALSSAINEVQKLKSKLFESESELEQSKYEV 184 Query: 502 KELEKLRKVYADTR 543 + LEKL + + R Sbjct: 185 RSLEKLVRQLEEER 198 >At3g53350.2 68416.m05887 myosin heavy chain-related low similarity to filamin-interacting protein S-FILIP [Rattus norvegicus] GI:21392397, nonmuscle heavy chain myosin II-A [Mus musculus] GI:17978023 Length = 394 Score = 30.3 bits (65), Expect = 3.6 Identities = 28/134 (20%), Positives = 63/134 (47%), Gaps = 1/134 (0%) Frame = +1 Query: 145 KVRQLESENSGLRREIQTTQEVVTREVSNIKGIYEHELQDAR-KLLDDTSREKAKLEIDL 321 ++ +LES S L+ E++ +E + R + +K + E +DA+ +L+D + E +++E +L Sbjct: 70 RIPELESTISQLQEELKKAKEELNRSEA-LKREAQEEAEDAKHQLMDINASEDSRIE-EL 127 Query: 322 KRLYXENXXXXXXXXXXXXXCQQAENLARHYETRFTEESNKYNTALADKKKAQDEARDLA 501 ++L E Q+ + + E K + L + + ++++ Sbjct: 128 RKLSQER---DKTWQSELEAMQRQHGMDSTALSSAINEVQKLKSKLFESESELEQSKYEV 184 Query: 502 KELEKLRKVYADTR 543 + LEKL + + R Sbjct: 185 RSLEKLVRQLEEER 198 >At4g02660.1 68417.m00361 WD-40 repeat family protein / beige-related contains Pfam PF00400: WD domain, G-beta repeat; similar to BEIGE (GI:3928547) [Rattus norvegicus]; lysosomal trafficking regulator - Bos taurus, EMBL: AF114785 Length = 3471 Score = 29.9 bits (64), Expect = 4.8 Identities = 13/22 (59%), Positives = 16/22 (72%) Frame = +1 Query: 274 LLDDTSREKAKLEIDLKRLYXE 339 LL TSR+K KLE+D KR + E Sbjct: 57 LLSPTSRDKLKLELDFKRYWEE 78 >At1g20960.1 68414.m02624 U5 small nuclear ribonucleoprotein helicase, putative similar to SP|O75643 U5 small nuclear ribonucleoprotein 200 kDa helicase {Homo sapiens}; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain, PF02889: Sec63 domain Length = 2171 Score = 29.9 bits (64), Expect = 4.8 Identities = 16/39 (41%), Positives = 24/39 (61%) Frame = +1 Query: 100 DALQNLNDRLAAYIDKVRQLESENSGLRREIQTTQEVVT 216 + + NL++RL Y VR+L + S REI+ TQ +VT Sbjct: 574 EVVGNLSNRLKDYGVIVRELSGDQSLTGREIEETQIIVT 612 >At1g04600.1 68414.m00454 myosin, putative similar to myosin (GI:499047) [Arabidopsis thaliana] Length = 1730 Score = 29.9 bits (64), Expect = 4.8 Identities = 16/60 (26%), Positives = 35/60 (58%) Frame = +1 Query: 145 KVRQLESENSGLRREIQTTQEVVTREVSNIKGIYEHELQDARKLLDDTSREKAKLEIDLK 324 ++ L+S + ++ +++ TQE ++E+S+++ + L D + L DT K+K DL+ Sbjct: 921 EIEALQSVLTDIKLQLRDTQETKSKEISDLQSV----LTDIKLQLRDTQETKSKEISDLQ 976 >At4g18200.1 68417.m02705 purine permease family protein similar to purine permease [Arabidopsis thaliana] GI:7620007; contains Pfam profile PF03151: Domain of unknown function, DUF250 Length = 1128 Score = 29.5 bits (63), Expect = 6.3 Identities = 15/41 (36%), Positives = 23/41 (56%), Gaps = 1/41 (2%) Frame = +2 Query: 65 GSYKTQPSSSRKMLYKISTIVLLLILTKF-VNWKVKTLGCV 184 G ++T PS R YK+ + +L L + W+V T+GCV Sbjct: 1012 GEWRTLPSEMRN--YKLGKVSYILTLASAAIFWQVYTVGCV 1050 >At3g05130.1 68416.m00557 expressed protein ; expression supported by MPSS Length = 634 Score = 29.5 bits (63), Expect = 6.3 Identities = 27/146 (18%), Positives = 60/146 (41%), Gaps = 7/146 (4%) Frame = +1 Query: 142 DKVRQLESENS-GLRREIQTTQEVVTREVSNIKG--IYEHELQDARKLLDDTSR----EK 300 DK E N L+ E+ ++++V + + ++ I L A+ L+ S E Sbjct: 445 DKALDEEKRNGEDLKAEVLKSEKMVAKTLEELEKVKIERKSLFSAKNDLESQSESLKSEN 504 Query: 301 AKLEIDLKRLYXENXXXXXXXXXXXXXCQQAENLARHYETRFTEESNKYNTALADKKKAQ 480 KLE +L L +++ + + + ++ N+ + +++++K + Sbjct: 505 VKLEKELVELRKAMEALKTELESAGMDAKRSMVMLKSAASMLSQLENREDRLISEEQKRE 564 Query: 481 DEARDLAKELEKLRKVYADTRXTLEE 558 A ELE + K + + +EE Sbjct: 565 IGTEPYAMELESIEKAFKNKEDIIEE 590 >At4g14760.1 68417.m02271 M protein repeat-containing protein contains Pfam profile: PF02370 M protein repeat Length = 1676 Score = 29.1 bits (62), Expect = 8.3 Identities = 39/179 (21%), Positives = 71/179 (39%), Gaps = 11/179 (6%) Frame = +1 Query: 106 LQNLNDRLAAYIDKVRQLESENSGLRREIQTTQEVVTREVSNIKGIYEHELQDARKLLDD 285 +++ + + + D+ Q E+E L++E+ EV E N++ Y+ L+ KL Sbjct: 274 IRDAEESVRVFRDQSEQAETEIKALKQELLKLNEV--NEDLNVR--YQQCLETISKL--- 326 Query: 286 TSREKAKLEIDLKRLYXENXXXXXXXXXXXXXCQ-----------QAENLARHYETRFTE 432 RE + + + KRL E C +AENLA + E Sbjct: 327 -EREVSHAQDNAKRLSSEVLAGAAKIKTVEEQCALLESFNQTMKVEAENLAHKMSAKDQE 385 Query: 433 ESNKYNTALADKKKAQDEARDLAKELEKLRKVYADTRXTLEEEMLCRIXMENTVXSLRE 609 S K N + Q+E ++ LR + + + EE+ + + + + LRE Sbjct: 386 LSQKQNEIEKLQAVMQEEQLRFSELGASLRNLESLHSQSQEEQKVLTSELHSRIQMLRE 444 >At3g45280.1 68416.m04889 syntaxin 72 (SYP72) identical to syntaxin of plants 72 (SYP72) (GI:13811650)[Arabidopsis thaliana]; identified as SYP72 in Sanderfoot, A.A., et al, Plant Physiology 124:1558-69(2000); syntaxin 8 - Homo sapiens, EMBL:AF115323 Length = 267 Score = 29.1 bits (62), Expect = 8.3 Identities = 16/57 (28%), Positives = 30/57 (52%), Gaps = 1/57 (1%) Frame = +1 Query: 427 TEESNKYNTALADKKKAQDEARDLAKE-LEKLRKVYADTRXTLEEEMLCRIXMENTV 594 +EES+++ ++K QDE D+ E L+ L+ + D L++++ ME V Sbjct: 162 SEESSQFRQEYEMRRKKQDEGLDIISEGLDALKNLARDMNEELDKQVPLMEEMETKV 218 >At3g44050.1 68416.m04718 kinesin motor protein-related KLP2 protein, Xenopus laevis, PIR:T30335 Length = 1229 Score = 29.1 bits (62), Expect = 8.3 Identities = 16/56 (28%), Positives = 31/56 (55%) Frame = +1 Query: 172 SGLRREIQTTQEVVTREVSNIKGIYEHELQDARKLLDDTSREKAKLEIDLKRLYXE 339 +G ++ +QE T+ S +K + +L RK +DD +++ KLE ++K + E Sbjct: 1053 TGKATDLLKSQEEKTKLQSEMK-LSREKLASVRKEVDDMTKKSLKLEKEIKTMETE 1107 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,645,106 Number of Sequences: 28952 Number of extensions: 218079 Number of successful extensions: 869 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 798 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 867 length of database: 12,070,560 effective HSP length: 84 effective length of database: 9,638,592 effective search space used: 4202426112 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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