Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030623sawa_D01_e4_07.seq
(1564 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g41140.1 68418.m05001 expressed protein 35 0.13
At5g52280.1 68418.m06488 protein transport protein-related low s... 35 0.17
At2g25350.1 68415.m03032 phox (PX) domain-containing protein wea... 34 0.29
At2g27170.1 68415.m06029 structural maintenance of chromosomes (... 33 0.68
At1g01660.1 68414.m00084 U-box domain-containing protein 32 1.2
At3g53350.1 68416.m05886 myosin heavy chain-related low similari... 31 2.7
At1g74830.1 68414.m08670 expressed protein contains Pfam profile... 31 2.7
At3g53350.3 68416.m05888 myosin heavy chain-related low similari... 30 3.6
At3g53350.2 68416.m05887 myosin heavy chain-related low similari... 30 3.6
At4g02660.1 68417.m00361 WD-40 repeat family protein / beige-rel... 30 4.8
At1g20960.1 68414.m02624 U5 small nuclear ribonucleoprotein heli... 30 4.8
At1g04600.1 68414.m00454 myosin, putative similar to myosin (GI:... 30 4.8
At4g18200.1 68417.m02705 purine permease family protein similar ... 29 6.3
At3g05130.1 68416.m00557 expressed protein ; expression supporte... 29 6.3
At4g14760.1 68417.m02271 M protein repeat-containing protein con... 29 8.3
At3g45280.1 68416.m04889 syntaxin 72 (SYP72) identical to syntax... 29 8.3
At3g44050.1 68416.m04718 kinesin motor protein-related KLP2 prot... 29 8.3
>At5g41140.1 68418.m05001 expressed protein
Length = 983
Score = 35.1 bits (77), Expect = 0.13
Identities = 36/170 (21%), Positives = 68/170 (40%), Gaps = 2/170 (1%)
Frame = +1
Query: 97 KDALQNLNDRLAAYIDKVRQLESENSGLRREIQTTQEVVTREVSNIKGIYEHELQDARKL 276
++ L N ND L +V ++E E E+ ++ T+E+ + E++ + +
Sbjct: 673 EELLMNANDEL-----RVNRVEYEAK--LNELSGKTDLKTKEMKRMSADLEYQKRQKEDV 725
Query: 277 LDDTSRE--KAKLEIDLKRLYXENXXXXXXXXXXXXXCQQAENLARHYETRFTEESNKYN 450
D + E + K EI++ RL E ++ + + E T ++
Sbjct: 726 NADLTHEITRRKDEIEILRLDLEETRKSSMETEASLS-EELQRIIDEKEAVITALKSQLE 784
Query: 451 TALADKKKAQDEARDLAKELEKLRKVYADTRXTLEEEMLCRIXMENTVXS 600
TA+A + + E+E LRK R LE++ +EN S
Sbjct: 785 TAIAPCDNLKHSLSNNESEIENLRKQVVQVRSELEKKEEEMANLENREAS 834
>At5g52280.1 68418.m06488 protein transport protein-related low
similarity to SP|P25386 Intracellular protein transport
protein USO1 {Saccharomyces cerevisiae}
Length = 853
Score = 34.7 bits (76), Expect = 0.17
Identities = 40/163 (24%), Positives = 71/163 (43%), Gaps = 3/163 (1%)
Frame = +1
Query: 79 TAVFK*KDALQNLNDRLAAYIDKVRQLESENSGLRRE-IQTTQEV--VTREVSNIKGIYE 249
++V + D ++ L L A + E E LR++ I+ ++ + +++EVS +KG +
Sbjct: 257 SSVTESSDPIERLKMELEALRRQSELSELEKQSLRKQAIKESKRIQELSKEVSCLKGERD 316
Query: 250 HELQDARKLLDDTSREKAKLEIDLKRLYXENXXXXXXXXXXXXXCQQAENLARHYETRFT 429
+++ KL SR++A E L R E+ C++ + + T
Sbjct: 317 GAMEECEKLRLQNSRDEADAESRL-RCISEDSSNMIEEIRDELSCEKDLTSNLKLQLQRT 375
Query: 430 EESNKYNTALADKKKAQDEARDLAKELEKLRKVYADTRXTLEE 558
+ESN N LA RDL + LE+ + LEE
Sbjct: 376 QESNS-NLILA--------VRDLNEMLEQKNNEISSLNSLLEE 409
>At2g25350.1 68415.m03032 phox (PX) domain-containing protein weak
similarity to SP|Q9UTK5 Abnormal long morphology protein
1 (Sp8) {Schizosaccharomyces pombe}; contains Pfam
profile PF00787: PX domain
Length = 643
Score = 33.9 bits (74), Expect = 0.29
Identities = 42/183 (22%), Positives = 75/183 (40%), Gaps = 1/183 (0%)
Frame = +1
Query: 61 ARVLQDTAVFK*KDALQNLNDRLAAYIDKVRQLESENSGLRREIQTTQEVVTREVSNIKG 240
AR+ Q+TAV K+ L D L ++ +Q EN L + + T ++ VT+ +++
Sbjct: 404 ARLNQETAV---KEYLNRKVDDLEVELETTKQRNKEN--LEQALMTERQSVTKMQWDMEE 458
Query: 241 IYEHELQDARKLLDDTSREKAKLEIDLKRLYXENXXXXXXXXXXXXXC-QQAENLARHYE 417
+ + + KL + K D K QQ E+L+R Y
Sbjct: 459 LRQKTFEMELKL-----KSKEDGSSDSKTSGNSTISESHELLQEMDATKQQLEDLSRRYV 513
Query: 418 TRFTEESNKYNTALADKKKAQDEARDLAKELEKLRKVYADTRXTLEEEMLCRIXMENTVX 597
+ + + K + ++ KEL + +DT L++E RI +ENT+
Sbjct: 514 ELEAKSKADIKVLVREVKSLRRSHMEMEKELTRSLTEKSDTEKLLQQE---RIIVENTLE 570
Query: 598 SLR 606
+ R
Sbjct: 571 ARR 573
>At2g27170.1 68415.m06029 structural maintenance of chromosomes
(SMC) family protein similar to basement
membrane-associated chondroitin proteoglycan Bamacan
[Rattus norvegicus] GI:1785540; contains Pfam profile
PF02463: RecF/RecN/SMC N terminal domain. No suitalble
start codon was identified.
Length = 1207
Score = 32.7 bits (71), Expect = 0.68
Identities = 28/122 (22%), Positives = 55/122 (45%), Gaps = 2/122 (1%)
Frame = +1
Query: 136 YID-KVRQLESENSGLRREIQTTQEVVTREVSNIKGIYEHELQDARKLLDDTSREKAKLE 312
Y+D ++R+L+ E LR+ Q ++ + E + IY+ EL DAR+ L+ + K
Sbjct: 195 YLDERLRELDEEKEELRKYQQLDKQRKSLEYT----IYDKELHDAREKLEQVEVARTKAS 250
Query: 313 IDLKRLYXENXXXXXXXXXXXXXCQQ-AENLARHYETRFTEESNKYNTALADKKKAQDEA 489
+ ++Y ++ + L Y+ + T E+ + AL K K + +
Sbjct: 251 EESTKMYDRVEKAQDDSKSLDESLKELTKELQTLYKEKETVEAQQ-TKALKKKTKLELDV 309
Query: 490 RD 495
+D
Sbjct: 310 KD 311
>At1g01660.1 68414.m00084 U-box domain-containing protein
Length = 568
Score = 31.9 bits (69), Expect = 1.2
Identities = 39/187 (20%), Positives = 78/187 (41%), Gaps = 15/187 (8%)
Frame = +1
Query: 97 KDALQNLND-RLAAYIDKVRQLESENSGLRREIQTTQ-EVVTREVSNIKGIYEHELQDAR 270
K AL + + + AY + VR+ ++EN+ + + E + E + ++ + L R
Sbjct: 231 KQALMEVEESKREAYEECVRRFKAENTAVEAIRSAREYEAMYNEEAKLRKEGKEALAKQR 290
Query: 271 KLLDDTSREKAKLEIDL---KRLYXENXXXXXXXXXXXXX-CQQAENLARHYET--RFTE 432
K+++ T +E+ I + ++LY E ++ E + E +
Sbjct: 291 KMVEKTKQERDDALIIILNGRKLYNEELRRRVEAEEMLGKEKEEHERTKKEIEEVRAIVQ 350
Query: 433 ESNKYNTALADKKKAQDEARDLAKELEKLRK-------VYADTRXTLEEEMLCRIXMENT 591
+ YN L +K+ ++ + +ELEK +K + + E+E+ R E
Sbjct: 351 DGTLYNEQLRHRKEMEESMKRQEEELEKTKKEKEEACMISKNLMQLYEDEVRQRKEAEEL 410
Query: 592 VXSLREE 612
V REE
Sbjct: 411 VKRRREE 417
>At3g53350.1 68416.m05886 myosin heavy chain-related low similarity
to filamin-interacting protein S-FILIP [Rattus
norvegicus] GI:21392397, nonmuscle heavy chain myosin
II-A [Mus musculus] GI:17978023
Length = 396
Score = 30.7 bits (66), Expect = 2.7
Identities = 30/144 (20%), Positives = 66/144 (45%), Gaps = 1/144 (0%)
Frame = +1
Query: 115 LNDRLAAYIDKVRQLESENSGLRREIQTTQEVVTREVSNIKGIYEHELQDAR-KLLDDTS 291
LN+ ++ +LES S L+ E++ +E + R + +K + E +DA+ +L+D +
Sbjct: 62 LNEIQKKRTGRIPELESTISQLQEELKKAKEELNRSEA-LKREAQEEAEDAKHQLMDINA 120
Query: 292 REKAKLEIDLKRLYXENXXXXXXXXXXXXXCQQAENLARHYETRFTEESNKYNTALADKK 471
E +++E +L++L E Q+ + + E K + L + +
Sbjct: 121 SEDSRIE-ELRKLSQER---DKTWQSELEAMQRQHGMDSTALSSAINEVQKLKSKLFESE 176
Query: 472 KAQDEARDLAKELEKLRKVYADTR 543
++++ + LEKL + + R
Sbjct: 177 SELEQSKYEVRSLEKLVRQLEEER 200
>At1g74830.1 68414.m08670 expressed protein contains Pfam profile
PF04576: Protein of unknown function, DUF593
Length = 542
Score = 30.7 bits (66), Expect = 2.7
Identities = 12/45 (26%), Positives = 26/45 (57%)
Frame = +1
Query: 385 QQAENLARHYETRFTEESNKYNTALADKKKAQDEARDLAKELEKL 519
+ EN+ ++ +++ +EES N AD++K + + KEL ++
Sbjct: 445 ENGENIDQNGQSKRSEESTAENVVSADEEKGSESKEGIVKELSEI 489
>At3g53350.3 68416.m05888 myosin heavy chain-related low similarity
to filamin-interacting protein S-FILIP [Rattus
norvegicus] GI:21392397, nonmuscle heavy chain myosin
II-A [Mus musculus] GI:17978023
Length = 394
Score = 30.3 bits (65), Expect = 3.6
Identities = 28/134 (20%), Positives = 63/134 (47%), Gaps = 1/134 (0%)
Frame = +1
Query: 145 KVRQLESENSGLRREIQTTQEVVTREVSNIKGIYEHELQDAR-KLLDDTSREKAKLEIDL 321
++ +LES S L+ E++ +E + R + +K + E +DA+ +L+D + E +++E +L
Sbjct: 70 RIPELESTISQLQEELKKAKEELNRSEA-LKREAQEEAEDAKHQLMDINASEDSRIE-EL 127
Query: 322 KRLYXENXXXXXXXXXXXXXCQQAENLARHYETRFTEESNKYNTALADKKKAQDEARDLA 501
++L E Q+ + + E K + L + + ++++
Sbjct: 128 RKLSQER---DKTWQSELEAMQRQHGMDSTALSSAINEVQKLKSKLFESESELEQSKYEV 184
Query: 502 KELEKLRKVYADTR 543
+ LEKL + + R
Sbjct: 185 RSLEKLVRQLEEER 198
>At3g53350.2 68416.m05887 myosin heavy chain-related low similarity
to filamin-interacting protein S-FILIP [Rattus
norvegicus] GI:21392397, nonmuscle heavy chain myosin
II-A [Mus musculus] GI:17978023
Length = 394
Score = 30.3 bits (65), Expect = 3.6
Identities = 28/134 (20%), Positives = 63/134 (47%), Gaps = 1/134 (0%)
Frame = +1
Query: 145 KVRQLESENSGLRREIQTTQEVVTREVSNIKGIYEHELQDAR-KLLDDTSREKAKLEIDL 321
++ +LES S L+ E++ +E + R + +K + E +DA+ +L+D + E +++E +L
Sbjct: 70 RIPELESTISQLQEELKKAKEELNRSEA-LKREAQEEAEDAKHQLMDINASEDSRIE-EL 127
Query: 322 KRLYXENXXXXXXXXXXXXXCQQAENLARHYETRFTEESNKYNTALADKKKAQDEARDLA 501
++L E Q+ + + E K + L + + ++++
Sbjct: 128 RKLSQER---DKTWQSELEAMQRQHGMDSTALSSAINEVQKLKSKLFESESELEQSKYEV 184
Query: 502 KELEKLRKVYADTR 543
+ LEKL + + R
Sbjct: 185 RSLEKLVRQLEEER 198
>At4g02660.1 68417.m00361 WD-40 repeat family protein /
beige-related contains Pfam PF00400: WD domain, G-beta
repeat; similar to BEIGE (GI:3928547) [Rattus
norvegicus]; lysosomal trafficking regulator - Bos
taurus, EMBL: AF114785
Length = 3471
Score = 29.9 bits (64), Expect = 4.8
Identities = 13/22 (59%), Positives = 16/22 (72%)
Frame = +1
Query: 274 LLDDTSREKAKLEIDLKRLYXE 339
LL TSR+K KLE+D KR + E
Sbjct: 57 LLSPTSRDKLKLELDFKRYWEE 78
>At1g20960.1 68414.m02624 U5 small nuclear ribonucleoprotein
helicase, putative similar to SP|O75643 U5 small nuclear
ribonucleoprotein 200 kDa helicase {Homo sapiens};
contains Pfam profiles PF00270: DEAD/DEAH box helicase,
PF00271: Helicase conserved C-terminal domain, PF02889:
Sec63 domain
Length = 2171
Score = 29.9 bits (64), Expect = 4.8
Identities = 16/39 (41%), Positives = 24/39 (61%)
Frame = +1
Query: 100 DALQNLNDRLAAYIDKVRQLESENSGLRREIQTTQEVVT 216
+ + NL++RL Y VR+L + S REI+ TQ +VT
Sbjct: 574 EVVGNLSNRLKDYGVIVRELSGDQSLTGREIEETQIIVT 612
>At1g04600.1 68414.m00454 myosin, putative similar to myosin
(GI:499047) [Arabidopsis thaliana]
Length = 1730
Score = 29.9 bits (64), Expect = 4.8
Identities = 16/60 (26%), Positives = 35/60 (58%)
Frame = +1
Query: 145 KVRQLESENSGLRREIQTTQEVVTREVSNIKGIYEHELQDARKLLDDTSREKAKLEIDLK 324
++ L+S + ++ +++ TQE ++E+S+++ + L D + L DT K+K DL+
Sbjct: 921 EIEALQSVLTDIKLQLRDTQETKSKEISDLQSV----LTDIKLQLRDTQETKSKEISDLQ 976
>At4g18200.1 68417.m02705 purine permease family protein similar to
purine permease [Arabidopsis thaliana] GI:7620007;
contains Pfam profile PF03151: Domain of unknown
function, DUF250
Length = 1128
Score = 29.5 bits (63), Expect = 6.3
Identities = 15/41 (36%), Positives = 23/41 (56%), Gaps = 1/41 (2%)
Frame = +2
Query: 65 GSYKTQPSSSRKMLYKISTIVLLLILTKF-VNWKVKTLGCV 184
G ++T PS R YK+ + +L L + W+V T+GCV
Sbjct: 1012 GEWRTLPSEMRN--YKLGKVSYILTLASAAIFWQVYTVGCV 1050
>At3g05130.1 68416.m00557 expressed protein ; expression supported
by MPSS
Length = 634
Score = 29.5 bits (63), Expect = 6.3
Identities = 27/146 (18%), Positives = 60/146 (41%), Gaps = 7/146 (4%)
Frame = +1
Query: 142 DKVRQLESENS-GLRREIQTTQEVVTREVSNIKG--IYEHELQDARKLLDDTSR----EK 300
DK E N L+ E+ ++++V + + ++ I L A+ L+ S E
Sbjct: 445 DKALDEEKRNGEDLKAEVLKSEKMVAKTLEELEKVKIERKSLFSAKNDLESQSESLKSEN 504
Query: 301 AKLEIDLKRLYXENXXXXXXXXXXXXXCQQAENLARHYETRFTEESNKYNTALADKKKAQ 480
KLE +L L +++ + + + ++ N+ + +++++K +
Sbjct: 505 VKLEKELVELRKAMEALKTELESAGMDAKRSMVMLKSAASMLSQLENREDRLISEEQKRE 564
Query: 481 DEARDLAKELEKLRKVYADTRXTLEE 558
A ELE + K + + +EE
Sbjct: 565 IGTEPYAMELESIEKAFKNKEDIIEE 590
>At4g14760.1 68417.m02271 M protein repeat-containing protein
contains Pfam profile: PF02370 M protein repeat
Length = 1676
Score = 29.1 bits (62), Expect = 8.3
Identities = 39/179 (21%), Positives = 71/179 (39%), Gaps = 11/179 (6%)
Frame = +1
Query: 106 LQNLNDRLAAYIDKVRQLESENSGLRREIQTTQEVVTREVSNIKGIYEHELQDARKLLDD 285
+++ + + + D+ Q E+E L++E+ EV E N++ Y+ L+ KL
Sbjct: 274 IRDAEESVRVFRDQSEQAETEIKALKQELLKLNEV--NEDLNVR--YQQCLETISKL--- 326
Query: 286 TSREKAKLEIDLKRLYXENXXXXXXXXXXXXXCQ-----------QAENLARHYETRFTE 432
RE + + + KRL E C +AENLA + E
Sbjct: 327 -EREVSHAQDNAKRLSSEVLAGAAKIKTVEEQCALLESFNQTMKVEAENLAHKMSAKDQE 385
Query: 433 ESNKYNTALADKKKAQDEARDLAKELEKLRKVYADTRXTLEEEMLCRIXMENTVXSLRE 609
S K N + Q+E ++ LR + + + EE+ + + + + LRE
Sbjct: 386 LSQKQNEIEKLQAVMQEEQLRFSELGASLRNLESLHSQSQEEQKVLTSELHSRIQMLRE 444
>At3g45280.1 68416.m04889 syntaxin 72 (SYP72) identical to syntaxin
of plants 72 (SYP72) (GI:13811650)[Arabidopsis
thaliana]; identified as SYP72 in Sanderfoot, A.A., et
al, Plant Physiology 124:1558-69(2000); syntaxin 8 -
Homo sapiens, EMBL:AF115323
Length = 267
Score = 29.1 bits (62), Expect = 8.3
Identities = 16/57 (28%), Positives = 30/57 (52%), Gaps = 1/57 (1%)
Frame = +1
Query: 427 TEESNKYNTALADKKKAQDEARDLAKE-LEKLRKVYADTRXTLEEEMLCRIXMENTV 594
+EES+++ ++K QDE D+ E L+ L+ + D L++++ ME V
Sbjct: 162 SEESSQFRQEYEMRRKKQDEGLDIISEGLDALKNLARDMNEELDKQVPLMEEMETKV 218
>At3g44050.1 68416.m04718 kinesin motor protein-related KLP2 protein,
Xenopus laevis, PIR:T30335
Length = 1229
Score = 29.1 bits (62), Expect = 8.3
Identities = 16/56 (28%), Positives = 31/56 (55%)
Frame = +1
Query: 172 SGLRREIQTTQEVVTREVSNIKGIYEHELQDARKLLDDTSREKAKLEIDLKRLYXE 339
+G ++ +QE T+ S +K + +L RK +DD +++ KLE ++K + E
Sbjct: 1053 TGKATDLLKSQEEKTKLQSEMK-LSREKLASVRKEVDDMTKKSLKLEKEIKTMETE 1107
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,645,106
Number of Sequences: 28952
Number of extensions: 218079
Number of successful extensions: 869
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 798
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 867
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4202426112
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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