SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030623sawa_D01_e4_07.seq
         (1564 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g41140.1 68418.m05001 expressed protein                             35   0.13 
At5g52280.1 68418.m06488 protein transport protein-related low s...    35   0.17 
At2g25350.1 68415.m03032 phox (PX) domain-containing protein wea...    34   0.29 
At2g27170.1 68415.m06029 structural maintenance of chromosomes (...    33   0.68 
At1g01660.1 68414.m00084 U-box domain-containing protein               32   1.2  
At3g53350.1 68416.m05886 myosin heavy chain-related low similari...    31   2.7  
At1g74830.1 68414.m08670 expressed protein contains Pfam profile...    31   2.7  
At3g53350.3 68416.m05888 myosin heavy chain-related low similari...    30   3.6  
At3g53350.2 68416.m05887 myosin heavy chain-related low similari...    30   3.6  
At4g02660.1 68417.m00361 WD-40 repeat family protein / beige-rel...    30   4.8  
At1g20960.1 68414.m02624 U5 small nuclear ribonucleoprotein heli...    30   4.8  
At1g04600.1 68414.m00454 myosin, putative similar to myosin (GI:...    30   4.8  
At4g18200.1 68417.m02705 purine permease family protein similar ...    29   6.3  
At3g05130.1 68416.m00557 expressed protein ; expression supporte...    29   6.3  
At4g14760.1 68417.m02271 M protein repeat-containing protein con...    29   8.3  
At3g45280.1 68416.m04889 syntaxin 72 (SYP72) identical to syntax...    29   8.3  
At3g44050.1 68416.m04718 kinesin motor protein-related KLP2 prot...    29   8.3  

>At5g41140.1 68418.m05001 expressed protein
          Length = 983

 Score = 35.1 bits (77), Expect = 0.13
 Identities = 36/170 (21%), Positives = 68/170 (40%), Gaps = 2/170 (1%)
 Frame = +1

Query: 97   KDALQNLNDRLAAYIDKVRQLESENSGLRREIQTTQEVVTREVSNIKGIYEHELQDARKL 276
            ++ L N ND L     +V ++E E      E+    ++ T+E+  +    E++ +    +
Sbjct: 673  EELLMNANDEL-----RVNRVEYEAK--LNELSGKTDLKTKEMKRMSADLEYQKRQKEDV 725

Query: 277  LDDTSRE--KAKLEIDLKRLYXENXXXXXXXXXXXXXCQQAENLARHYETRFTEESNKYN 450
              D + E  + K EI++ RL  E               ++ + +    E   T   ++  
Sbjct: 726  NADLTHEITRRKDEIEILRLDLEETRKSSMETEASLS-EELQRIIDEKEAVITALKSQLE 784

Query: 451  TALADKKKAQDEARDLAKELEKLRKVYADTRXTLEEEMLCRIXMENTVXS 600
            TA+A     +    +   E+E LRK     R  LE++      +EN   S
Sbjct: 785  TAIAPCDNLKHSLSNNESEIENLRKQVVQVRSELEKKEEEMANLENREAS 834


>At5g52280.1 68418.m06488 protein transport protein-related low
           similarity to  SP|P25386 Intracellular protein transport
           protein USO1 {Saccharomyces cerevisiae}
          Length = 853

 Score = 34.7 bits (76), Expect = 0.17
 Identities = 40/163 (24%), Positives = 71/163 (43%), Gaps = 3/163 (1%)
 Frame = +1

Query: 79  TAVFK*KDALQNLNDRLAAYIDKVRQLESENSGLRRE-IQTTQEV--VTREVSNIKGIYE 249
           ++V +  D ++ L   L A   +    E E   LR++ I+ ++ +  +++EVS +KG  +
Sbjct: 257 SSVTESSDPIERLKMELEALRRQSELSELEKQSLRKQAIKESKRIQELSKEVSCLKGERD 316

Query: 250 HELQDARKLLDDTSREKAKLEIDLKRLYXENXXXXXXXXXXXXXCQQAENLARHYETRFT 429
             +++  KL    SR++A  E  L R   E+             C++        + + T
Sbjct: 317 GAMEECEKLRLQNSRDEADAESRL-RCISEDSSNMIEEIRDELSCEKDLTSNLKLQLQRT 375

Query: 430 EESNKYNTALADKKKAQDEARDLAKELEKLRKVYADTRXTLEE 558
           +ESN  N  LA         RDL + LE+     +     LEE
Sbjct: 376 QESNS-NLILA--------VRDLNEMLEQKNNEISSLNSLLEE 409


>At2g25350.1 68415.m03032 phox (PX) domain-containing protein weak
           similarity to SP|Q9UTK5 Abnormal long morphology protein
           1 (Sp8) {Schizosaccharomyces pombe}; contains Pfam
           profile PF00787: PX domain
          Length = 643

 Score = 33.9 bits (74), Expect = 0.29
 Identities = 42/183 (22%), Positives = 75/183 (40%), Gaps = 1/183 (0%)
 Frame = +1

Query: 61  ARVLQDTAVFK*KDALQNLNDRLAAYIDKVRQLESENSGLRREIQTTQEVVTREVSNIKG 240
           AR+ Q+TAV   K+ L    D L   ++  +Q   EN  L + + T ++ VT+   +++ 
Sbjct: 404 ARLNQETAV---KEYLNRKVDDLEVELETTKQRNKEN--LEQALMTERQSVTKMQWDMEE 458

Query: 241 IYEHELQDARKLLDDTSREKAKLEIDLKRLYXENXXXXXXXXXXXXXC-QQAENLARHYE 417
           + +   +   KL     + K     D K                     QQ E+L+R Y 
Sbjct: 459 LRQKTFEMELKL-----KSKEDGSSDSKTSGNSTISESHELLQEMDATKQQLEDLSRRYV 513

Query: 418 TRFTEESNKYNTALADKKKAQDEARDLAKELEKLRKVYADTRXTLEEEMLCRIXMENTVX 597
               +        + + K  +    ++ KEL +     +DT   L++E   RI +ENT+ 
Sbjct: 514 ELEAKSKADIKVLVREVKSLRRSHMEMEKELTRSLTEKSDTEKLLQQE---RIIVENTLE 570

Query: 598 SLR 606
           + R
Sbjct: 571 ARR 573


>At2g27170.1 68415.m06029 structural maintenance of chromosomes
           (SMC) family protein similar to basement
           membrane-associated chondroitin proteoglycan Bamacan
           [Rattus norvegicus] GI:1785540; contains Pfam profile
           PF02463: RecF/RecN/SMC N terminal domain. No suitalble
           start codon was identified.
          Length = 1207

 Score = 32.7 bits (71), Expect = 0.68
 Identities = 28/122 (22%), Positives = 55/122 (45%), Gaps = 2/122 (1%)
 Frame = +1

Query: 136 YID-KVRQLESENSGLRREIQTTQEVVTREVSNIKGIYEHELQDARKLLDDTSREKAKLE 312
           Y+D ++R+L+ E   LR+  Q  ++  + E +    IY+ EL DAR+ L+     + K  
Sbjct: 195 YLDERLRELDEEKEELRKYQQLDKQRKSLEYT----IYDKELHDAREKLEQVEVARTKAS 250

Query: 313 IDLKRLYXENXXXXXXXXXXXXXCQQ-AENLARHYETRFTEESNKYNTALADKKKAQDEA 489
            +  ++Y                 ++  + L   Y+ + T E+ +   AL  K K + + 
Sbjct: 251 EESTKMYDRVEKAQDDSKSLDESLKELTKELQTLYKEKETVEAQQ-TKALKKKTKLELDV 309

Query: 490 RD 495
           +D
Sbjct: 310 KD 311


>At1g01660.1 68414.m00084 U-box domain-containing protein
          Length = 568

 Score = 31.9 bits (69), Expect = 1.2
 Identities = 39/187 (20%), Positives = 78/187 (41%), Gaps = 15/187 (8%)
 Frame = +1

Query: 97  KDALQNLND-RLAAYIDKVRQLESENSGLRREIQTTQ-EVVTREVSNIKGIYEHELQDAR 270
           K AL  + + +  AY + VR+ ++EN+ +       + E +  E + ++   +  L   R
Sbjct: 231 KQALMEVEESKREAYEECVRRFKAENTAVEAIRSAREYEAMYNEEAKLRKEGKEALAKQR 290

Query: 271 KLLDDTSREKAKLEIDL---KRLYXENXXXXXXXXXXXXX-CQQAENLARHYET--RFTE 432
           K+++ T +E+    I +   ++LY E                ++ E   +  E      +
Sbjct: 291 KMVEKTKQERDDALIIILNGRKLYNEELRRRVEAEEMLGKEKEEHERTKKEIEEVRAIVQ 350

Query: 433 ESNKYNTALADKKKAQDEARDLAKELEKLRK-------VYADTRXTLEEEMLCRIXMENT 591
           +   YN  L  +K+ ++  +   +ELEK +K       +  +     E+E+  R   E  
Sbjct: 351 DGTLYNEQLRHRKEMEESMKRQEEELEKTKKEKEEACMISKNLMQLYEDEVRQRKEAEEL 410

Query: 592 VXSLREE 612
           V   REE
Sbjct: 411 VKRRREE 417


>At3g53350.1 68416.m05886 myosin heavy chain-related low similarity
           to filamin-interacting protein S-FILIP [Rattus
           norvegicus] GI:21392397, nonmuscle heavy chain myosin
           II-A [Mus musculus] GI:17978023
          Length = 396

 Score = 30.7 bits (66), Expect = 2.7
 Identities = 30/144 (20%), Positives = 66/144 (45%), Gaps = 1/144 (0%)
 Frame = +1

Query: 115 LNDRLAAYIDKVRQLESENSGLRREIQTTQEVVTREVSNIKGIYEHELQDAR-KLLDDTS 291
           LN+       ++ +LES  S L+ E++  +E + R  + +K   + E +DA+ +L+D  +
Sbjct: 62  LNEIQKKRTGRIPELESTISQLQEELKKAKEELNRSEA-LKREAQEEAEDAKHQLMDINA 120

Query: 292 REKAKLEIDLKRLYXENXXXXXXXXXXXXXCQQAENLARHYETRFTEESNKYNTALADKK 471
            E +++E +L++L  E               Q+   +     +    E  K  + L + +
Sbjct: 121 SEDSRIE-ELRKLSQER---DKTWQSELEAMQRQHGMDSTALSSAINEVQKLKSKLFESE 176

Query: 472 KAQDEARDLAKELEKLRKVYADTR 543
              ++++   + LEKL +   + R
Sbjct: 177 SELEQSKYEVRSLEKLVRQLEEER 200


>At1g74830.1 68414.m08670 expressed protein contains Pfam profile
           PF04576: Protein of unknown function, DUF593
          Length = 542

 Score = 30.7 bits (66), Expect = 2.7
 Identities = 12/45 (26%), Positives = 26/45 (57%)
 Frame = +1

Query: 385 QQAENLARHYETRFTEESNKYNTALADKKKAQDEARDLAKELEKL 519
           +  EN+ ++ +++ +EES   N   AD++K  +    + KEL ++
Sbjct: 445 ENGENIDQNGQSKRSEESTAENVVSADEEKGSESKEGIVKELSEI 489


>At3g53350.3 68416.m05888 myosin heavy chain-related low similarity
           to filamin-interacting protein S-FILIP [Rattus
           norvegicus] GI:21392397, nonmuscle heavy chain myosin
           II-A [Mus musculus] GI:17978023
          Length = 394

 Score = 30.3 bits (65), Expect = 3.6
 Identities = 28/134 (20%), Positives = 63/134 (47%), Gaps = 1/134 (0%)
 Frame = +1

Query: 145 KVRQLESENSGLRREIQTTQEVVTREVSNIKGIYEHELQDAR-KLLDDTSREKAKLEIDL 321
           ++ +LES  S L+ E++  +E + R  + +K   + E +DA+ +L+D  + E +++E +L
Sbjct: 70  RIPELESTISQLQEELKKAKEELNRSEA-LKREAQEEAEDAKHQLMDINASEDSRIE-EL 127

Query: 322 KRLYXENXXXXXXXXXXXXXCQQAENLARHYETRFTEESNKYNTALADKKKAQDEARDLA 501
           ++L  E               Q+   +     +    E  K  + L + +   ++++   
Sbjct: 128 RKLSQER---DKTWQSELEAMQRQHGMDSTALSSAINEVQKLKSKLFESESELEQSKYEV 184

Query: 502 KELEKLRKVYADTR 543
           + LEKL +   + R
Sbjct: 185 RSLEKLVRQLEEER 198


>At3g53350.2 68416.m05887 myosin heavy chain-related low similarity
           to filamin-interacting protein S-FILIP [Rattus
           norvegicus] GI:21392397, nonmuscle heavy chain myosin
           II-A [Mus musculus] GI:17978023
          Length = 394

 Score = 30.3 bits (65), Expect = 3.6
 Identities = 28/134 (20%), Positives = 63/134 (47%), Gaps = 1/134 (0%)
 Frame = +1

Query: 145 KVRQLESENSGLRREIQTTQEVVTREVSNIKGIYEHELQDAR-KLLDDTSREKAKLEIDL 321
           ++ +LES  S L+ E++  +E + R  + +K   + E +DA+ +L+D  + E +++E +L
Sbjct: 70  RIPELESTISQLQEELKKAKEELNRSEA-LKREAQEEAEDAKHQLMDINASEDSRIE-EL 127

Query: 322 KRLYXENXXXXXXXXXXXXXCQQAENLARHYETRFTEESNKYNTALADKKKAQDEARDLA 501
           ++L  E               Q+   +     +    E  K  + L + +   ++++   
Sbjct: 128 RKLSQER---DKTWQSELEAMQRQHGMDSTALSSAINEVQKLKSKLFESESELEQSKYEV 184

Query: 502 KELEKLRKVYADTR 543
           + LEKL +   + R
Sbjct: 185 RSLEKLVRQLEEER 198


>At4g02660.1 68417.m00361 WD-40 repeat family protein /
           beige-related contains Pfam PF00400: WD domain, G-beta
           repeat; similar to BEIGE (GI:3928547) [Rattus
           norvegicus]; lysosomal trafficking regulator - Bos
           taurus, EMBL: AF114785
          Length = 3471

 Score = 29.9 bits (64), Expect = 4.8
 Identities = 13/22 (59%), Positives = 16/22 (72%)
 Frame = +1

Query: 274 LLDDTSREKAKLEIDLKRLYXE 339
           LL  TSR+K KLE+D KR + E
Sbjct: 57  LLSPTSRDKLKLELDFKRYWEE 78


>At1g20960.1 68414.m02624 U5 small nuclear ribonucleoprotein
           helicase, putative similar to SP|O75643 U5 small nuclear
           ribonucleoprotein 200 kDa helicase {Homo sapiens};
           contains Pfam profiles PF00270: DEAD/DEAH box helicase,
           PF00271: Helicase conserved C-terminal domain, PF02889:
           Sec63 domain
          Length = 2171

 Score = 29.9 bits (64), Expect = 4.8
 Identities = 16/39 (41%), Positives = 24/39 (61%)
 Frame = +1

Query: 100 DALQNLNDRLAAYIDKVRQLESENSGLRREIQTTQEVVT 216
           + + NL++RL  Y   VR+L  + S   REI+ TQ +VT
Sbjct: 574 EVVGNLSNRLKDYGVIVRELSGDQSLTGREIEETQIIVT 612


>At1g04600.1 68414.m00454 myosin, putative similar to myosin
            (GI:499047) [Arabidopsis thaliana]
          Length = 1730

 Score = 29.9 bits (64), Expect = 4.8
 Identities = 16/60 (26%), Positives = 35/60 (58%)
 Frame = +1

Query: 145  KVRQLESENSGLRREIQTTQEVVTREVSNIKGIYEHELQDARKLLDDTSREKAKLEIDLK 324
            ++  L+S  + ++ +++ TQE  ++E+S+++ +    L D +  L DT   K+K   DL+
Sbjct: 921  EIEALQSVLTDIKLQLRDTQETKSKEISDLQSV----LTDIKLQLRDTQETKSKEISDLQ 976


>At4g18200.1 68417.m02705 purine permease family protein similar to
            purine permease [Arabidopsis thaliana] GI:7620007;
            contains Pfam profile PF03151: Domain of unknown
            function, DUF250
          Length = 1128

 Score = 29.5 bits (63), Expect = 6.3
 Identities = 15/41 (36%), Positives = 23/41 (56%), Gaps = 1/41 (2%)
 Frame = +2

Query: 65   GSYKTQPSSSRKMLYKISTIVLLLILTKF-VNWKVKTLGCV 184
            G ++T PS  R   YK+  +  +L L    + W+V T+GCV
Sbjct: 1012 GEWRTLPSEMRN--YKLGKVSYILTLASAAIFWQVYTVGCV 1050


>At3g05130.1 68416.m00557 expressed protein ; expression supported
           by MPSS
          Length = 634

 Score = 29.5 bits (63), Expect = 6.3
 Identities = 27/146 (18%), Positives = 60/146 (41%), Gaps = 7/146 (4%)
 Frame = +1

Query: 142 DKVRQLESENS-GLRREIQTTQEVVTREVSNIKG--IYEHELQDARKLLDDTSR----EK 300
           DK    E  N   L+ E+  ++++V + +  ++   I    L  A+  L+  S     E 
Sbjct: 445 DKALDEEKRNGEDLKAEVLKSEKMVAKTLEELEKVKIERKSLFSAKNDLESQSESLKSEN 504

Query: 301 AKLEIDLKRLYXENXXXXXXXXXXXXXCQQAENLARHYETRFTEESNKYNTALADKKKAQ 480
            KLE +L  L                  +++  + +   +  ++  N+ +  +++++K +
Sbjct: 505 VKLEKELVELRKAMEALKTELESAGMDAKRSMVMLKSAASMLSQLENREDRLISEEQKRE 564

Query: 481 DEARDLAKELEKLRKVYADTRXTLEE 558
                 A ELE + K + +    +EE
Sbjct: 565 IGTEPYAMELESIEKAFKNKEDIIEE 590


>At4g14760.1 68417.m02271 M protein repeat-containing protein
           contains Pfam profile: PF02370 M protein repeat
          Length = 1676

 Score = 29.1 bits (62), Expect = 8.3
 Identities = 39/179 (21%), Positives = 71/179 (39%), Gaps = 11/179 (6%)
 Frame = +1

Query: 106 LQNLNDRLAAYIDKVRQLESENSGLRREIQTTQEVVTREVSNIKGIYEHELQDARKLLDD 285
           +++  + +  + D+  Q E+E   L++E+    EV   E  N++  Y+  L+   KL   
Sbjct: 274 IRDAEESVRVFRDQSEQAETEIKALKQELLKLNEV--NEDLNVR--YQQCLETISKL--- 326

Query: 286 TSREKAKLEIDLKRLYXENXXXXXXXXXXXXXCQ-----------QAENLARHYETRFTE 432
             RE +  + + KRL  E              C            +AENLA     +  E
Sbjct: 327 -EREVSHAQDNAKRLSSEVLAGAAKIKTVEEQCALLESFNQTMKVEAENLAHKMSAKDQE 385

Query: 433 ESNKYNTALADKKKAQDEARDLAKELEKLRKVYADTRXTLEEEMLCRIXMENTVXSLRE 609
            S K N     +   Q+E    ++    LR + +    + EE+ +    + + +  LRE
Sbjct: 386 LSQKQNEIEKLQAVMQEEQLRFSELGASLRNLESLHSQSQEEQKVLTSELHSRIQMLRE 444


>At3g45280.1 68416.m04889 syntaxin 72 (SYP72) identical to syntaxin
           of plants 72 (SYP72) (GI:13811650)[Arabidopsis
           thaliana]; identified as SYP72 in Sanderfoot, A.A., et
           al, Plant Physiology 124:1558-69(2000); syntaxin 8 -
           Homo sapiens, EMBL:AF115323
          Length = 267

 Score = 29.1 bits (62), Expect = 8.3
 Identities = 16/57 (28%), Positives = 30/57 (52%), Gaps = 1/57 (1%)
 Frame = +1

Query: 427 TEESNKYNTALADKKKAQDEARDLAKE-LEKLRKVYADTRXTLEEEMLCRIXMENTV 594
           +EES+++      ++K QDE  D+  E L+ L+ +  D    L++++     ME  V
Sbjct: 162 SEESSQFRQEYEMRRKKQDEGLDIISEGLDALKNLARDMNEELDKQVPLMEEMETKV 218


>At3g44050.1 68416.m04718 kinesin motor protein-related KLP2 protein,
            Xenopus laevis, PIR:T30335
          Length = 1229

 Score = 29.1 bits (62), Expect = 8.3
 Identities = 16/56 (28%), Positives = 31/56 (55%)
 Frame = +1

Query: 172  SGLRREIQTTQEVVTREVSNIKGIYEHELQDARKLLDDTSREKAKLEIDLKRLYXE 339
            +G   ++  +QE  T+  S +K +   +L   RK +DD +++  KLE ++K +  E
Sbjct: 1053 TGKATDLLKSQEEKTKLQSEMK-LSREKLASVRKEVDDMTKKSLKLEKEIKTMETE 1107


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,645,106
Number of Sequences: 28952
Number of extensions: 218079
Number of successful extensions: 869
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 798
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 867
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4202426112
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -