Samia BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030623sawa_C06_e43_06.seq
         (1565 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g65590.1 68414.m07441 glycosyl hydrolase family 20 protein co...    87   2e-17
At3g55260.1 68416.m06137 glycosyl hydrolase family 20 protein si...    77   2e-14
At1g05590.1 68414.m00579 glycosyl hydrolase family 20 protein si...    53   4e-07
At2g36110.1 68415.m04434 3'-5' exonuclease domain-containing pro...    29   8.3  

>At1g65590.1 68414.m07441 glycosyl hydrolase family 20 protein
           contains Pfam PF00728: Glycosyl hydrolase family 20,
           catalytic domain; contains Pfam PF02838: Glycosyl
           hydrolase family 20, domain 2; similar to
           Beta-hexosaminidase beta chain precursor (EC 3.2.1.52)
           (N-acetyl-beta- glucosaminidase)
           (Beta-N-acetylhexosaminidase) (Hexosaminidase B)
           (Swiss-Prot:P07686) [Homo sapiens]
          Length = 535

 Score = 87.4 bits (207), Expect = 2e-17
 Identities = 46/127 (36%), Positives = 70/127 (55%), Gaps = 2/127 (1%)
 Frame = +3

Query: 135 HILSKGHKVVYSSA--WYLDHLMSGGDWTNFYLSDPRQMVRQYSPTANVDNIVGGEACMW 308
           ++ + G + + S+   WYLDH+     W  FY ++P Q +      + V   +GGE CMW
Sbjct: 405 NVTASGLRCIVSNQEFWYLDHI--DAPWQGFYANEPFQNITDKKQQSLV---LGGEVCMW 459

Query: 309 GEVVNDMNVISRIWSRASAVAERLWSPETEGLRYMNKVPNEVYSRLEEHTCRMNRRGIDA 488
           GE ++  ++   IW RA+A AERLW+P  +    + K PN V +RL    C +N+RG+ A
Sbjct: 460 GEHIDASDIEQTIWPRAAAAAERLWTPYAK----LAKNPNNVTTRLAHFRCLLNQRGVAA 515

Query: 489 QPPSGPG 509
            P  G G
Sbjct: 516 APLVGGG 522


>At3g55260.1 68416.m06137 glycosyl hydrolase family 20 protein
           similar to beta-hexosaminidase A SP:P13723 from
           [Dictyostelium discoideum]
          Length = 541

 Score = 77.4 bits (182), Expect = 2e-14
 Identities = 48/138 (34%), Positives = 66/138 (47%), Gaps = 11/138 (7%)
 Frame = +3

Query: 141 LSKGHKVVYSSA--WYLDHLMSGGDWTNFYLSDPRQMVRQYSPTANVDNIVGGEACMWGE 314
           ++KG + ++S+   WYLDHL     W   Y ++P   +   S    V   +GGE CMWGE
Sbjct: 410 VAKGFRCIFSNQGYWYLDHL--DVPWEEVYNTEPLNGIEDPSLQKLV---IGGEVCMWGE 464

Query: 315 VVNDMNVISRIWSRASAVAERLWSPETEGLRYMNKVPNEVYSRLEEHTCRMNRRGIDA-- 488
             +   V+  IW RA+A AER+WS  T        +      RL    C +N RG+ A  
Sbjct: 465 TADTSVVLQTIWPRAAAAAERMWS--TREAVSKGNITLTALPRLHYFRCLLNNRGVPAAP 522

Query: 489 -------QPPSGPGFCIA 521
                  +PP GPG C A
Sbjct: 523 VDNFYARRPPLGPGSCYA 540


>At1g05590.1 68414.m00579 glycosyl hydrolase family 20 protein
           similar to beta-hexosaminidase precursor SP:P43077 from
           [Candida albicans]
          Length = 580

 Score = 53.2 bits (122), Expect = 4e-07
 Identities = 31/82 (37%), Positives = 45/82 (54%)
 Frame = +3

Query: 282 IVGGEACMWGEVVNDMNVISRIWSRASAVAERLWSPETEGLRYMNKVPNEVYSRLEEHTC 461
           ++GGE  +W E  +   + SR+W RASA+AE LWS   +  R + +   E   RL     
Sbjct: 489 VLGGEVALWSEQADSTVLDSRLWPRASALAESLWSGNRDE-RGVKRC-GEAVDRLNLWRY 546

Query: 462 RMNRRGIDAQPPSGPGFCIALP 527
           RM +RGI A+P   P +C+  P
Sbjct: 547 RMVKRGIGAEPIQ-PLWCLKNP 567


>At2g36110.1 68415.m04434 3'-5' exonuclease domain-containing
           protein contains Pfam profile PF01612: 3'-5' exonuclease
          Length = 239

 Score = 29.1 bits (62), Expect = 8.3
 Identities = 13/38 (34%), Positives = 20/38 (52%)
 Frame = +3

Query: 285 VGGEACMWGEVVNDMNVISRIWSRASAVAERLWSPETE 398
           +G + C+W E     NV  RIW  +S V E +W   ++
Sbjct: 197 LGVKECIWKE---RSNVKERIWKESSNVKEHIWKESSK 231


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,055,108
Number of Sequences: 28952
Number of extensions: 354960
Number of successful extensions: 899
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 719
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 896
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4212064704
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)