BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030623sawa_B10_e74_04.seq (1430 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC13C5.03 |tht1||nuclear membrane protein involved in karyogam... 34 0.055 SPBC27.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||M... 29 1.6 SPAC17G6.16c |ysh1||mRNA cleavage and polyadenylation specificit... 29 2.1 SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit ... 28 2.8 SPAC17G6.06 |rps2401|rps24-1, rps24|40S ribosomal protein S24|Sc... 27 4.8 SPAC1565.05 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 27 4.8 >SPAC13C5.03 |tht1||nuclear membrane protein involved in karyogamy |Schizosaccharomyces pombe|chr 1|||Manual Length = 543 Score = 33.9 bits (74), Expect = 0.055 Identities = 18/45 (40%), Positives = 28/45 (62%), Gaps = 1/45 (2%) Frame = -1 Query: 188 LRVRTVFIQALNSGVISIFLTYKKNTNSKINS-LSVFLPRPNSCS 57 L +RT ++ A +++ FL Y K NSK ++ LS+FLP P C+ Sbjct: 442 LNIRTFYLTAFLCALLN-FLRYLKYRNSKKDTELSLFLPAPEECN 485 >SPBC27.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 1052 Score = 29.1 bits (62), Expect = 1.6 Identities = 31/125 (24%), Positives = 53/125 (42%) Frame = +1 Query: 334 RYLSFFNNESENDMSELDRDQIDTGAQRIINTCSHLLKEFRNDNRKVSVSDQTREYMDAV 513 R+L + E D D D + I+N L + FR DN S+ + Sbjct: 260 RHLELILKQIYEKKLEEDADAKDIAPRAIVNFWRLLTELFRGDNDTPEKSNALPD----- 314 Query: 514 IDLVDAYLKAVCKIHSELKALRVKRALDIRKLSRLELPQSKSIILNPFVSDKDPEKRKTL 693 D +D +LK+ I S L L + + + EL S ++ + V D++ +K K L Sbjct: 315 -DELDKHLKSDFDIESFCFLLH--HLLSLYQTQKKELASSNELLAS--VRDENTKKLKEL 369 Query: 694 QEDVD 708 Q+ ++ Sbjct: 370 QDIIE 374 >SPAC17G6.16c |ysh1||mRNA cleavage and polyadenylation specificity factor complex subunit Ysh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 775 Score = 28.7 bits (61), Expect = 2.1 Identities = 13/50 (26%), Positives = 27/50 (54%) Frame = +1 Query: 349 FNNESENDMSELDRDQIDTGAQRIINTCSHLLKEFRNDNRKVSVSDQTRE 498 F+ S+ND+SE D + ++ +I + + + +N+N+ V +Q E Sbjct: 712 FSEASQNDVSEDDFENEESDDDKIFEQQTKIEDDVKNENKTEPVEEQKSE 761 >SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit Bgs2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1894 Score = 28.3 bits (60), Expect = 2.8 Identities = 19/64 (29%), Positives = 33/64 (51%) Frame = +1 Query: 376 SELDRDQIDTGAQRIINTCSHLLKEFRNDNRKVSVSDQTREYMDAVIDLVDAYLKAVCKI 555 SELD ++ RI + + +L + ++DN+ +S+ EY+ + D YL+ KI Sbjct: 1095 SELDENKKRKPKYRIKLSGNPILGDGKSDNQNLSLPFYRGEYIQLIDANQDNYLEECLKI 1154 Query: 556 HSEL 567 S L Sbjct: 1155 RSIL 1158 >SPAC17G6.06 |rps2401|rps24-1, rps24|40S ribosomal protein S24|Schizosaccharomyces pombe|chr 1|||Manual Length = 134 Score = 27.5 bits (58), Expect = 4.8 Identities = 17/56 (30%), Positives = 30/56 (53%) Frame = +1 Query: 535 LKAVCKIHSELKALRVKRALDIRKLSRLELPQSKSIILNPFVSDKDPEKRKTLQED 702 ++A+ K + +RV A I+K++R + Q K+ F + K KRK+ Q+D Sbjct: 79 VEAMKKFEPHYRLVRVGHAEPIQKVARQQRKQRKNRGKKVFGTGKRLAKRKSKQQD 134 >SPAC1565.05 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 773 Score = 27.5 bits (58), Expect = 4.8 Identities = 17/77 (22%), Positives = 37/77 (48%) Frame = +1 Query: 391 DQIDTGAQRIINTCSHLLKEFRNDNRKVSVSDQTREYMDAVIDLVDAYLKAVCKIHSELK 570 D +R++NT + +LKE + + + D T Y ++ L ++ +H E K Sbjct: 249 DSFSVSERRLVNTRAVVLKEVQAPSHILMSKDSTSIYTISLEGL------SIYSLHDESK 302 Query: 571 ALRVKRALDIRKLSRLE 621 + + + L + +S++E Sbjct: 303 SYMLIKILHFQSISKIE 319 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,870,903 Number of Sequences: 5004 Number of extensions: 67587 Number of successful extensions: 191 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 182 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 191 length of database: 2,362,478 effective HSP length: 75 effective length of database: 1,987,178 effective search space used: 796858378 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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