BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030623sawa_B01_e2_03.seq (1560 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC211.04c |mcm6|mis5|MCM complex subunit Mcm6 |Schizosaccharom... 43 1e-04 SPAC1B2.05 |mcm5|nda4, SPAC3F10.01|MCM complex subunit Mcm5|Schi... 36 0.020 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 31 0.57 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 29 1.7 SPBC26H8.04c |||DEP domain|Schizosaccharomyces pombe|chr 2|||Manual 29 2.3 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 29 2.3 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 3.0 SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 27 7.0 SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 27 7.0 >SPBC211.04c |mcm6|mis5|MCM complex subunit Mcm6 |Schizosaccharomyces pombe|chr 2|||Manual Length = 892 Score = 43.2 bits (97), Expect = 1e-04 Identities = 28/108 (25%), Positives = 49/108 (45%), Gaps = 12/108 (11%) Frame = +1 Query: 439 QVKDEVGVRCQKLFQDFLEEFKEDN------------ELKYEKHAKELLKPELSTLEVSF 582 +V D G ++ F++FL F +D E Y + L E+ T+ V + Sbjct: 63 KVIDTTGESVREAFEEFLLSFSDDRVAGGDALPSASQEKYYVQQIHGLAMYEIHTVYVDY 122 Query: 583 DDVEKYNQNLATTIIEEYYRIYPFLNQAIXNYVLGFADXGLKKRITKQ 726 + YN LA I+E+YYR PFL +A+ + F + ++++ Sbjct: 123 KHLTSYNDVLALAIVEQYYRFSPFLLRALQKLIEKFEPEYYRSSLSRE 170 >SPAC1B2.05 |mcm5|nda4, SPAC3F10.01|MCM complex subunit Mcm5|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 35.5 bits (78), Expect = 0.020 Identities = 18/67 (26%), Positives = 32/67 (47%) Frame = +1 Query: 469 QKLFQDFLEEFKEDNELKYEKHAKELLKPELSTLEVSFDDVEKYNQNLATTIIEEYYRIY 648 +K F F+EEF DN+ Y ++ L + L + + YN++LA ++ + I Sbjct: 31 EKNFIQFIEEFVIDNDFIYRTQLRDNLVVKQYMLNIDLRHLISYNEDLAHLLLSQPTDIL 90 Query: 649 PFLNQAI 669 P A+ Sbjct: 91 PLFESAV 97 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 30.7 bits (66), Expect = 0.57 Identities = 16/49 (32%), Positives = 16/49 (32%) Frame = +1 Query: 1084 KXXXTEPEXPPARXPXXXPGPLXGPXGGPXPRAXXPPXPXPPGXXGGXG 1230 K P PP P P P P P PP P PP G G Sbjct: 726 KLLLKSPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAG 774 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 29.1 bits (62), Expect = 1.7 Identities = 16/50 (32%), Positives = 16/50 (32%) Frame = +1 Query: 1057 PXGGXPGXXKXXXTEPEXPPARXPXXXPGPLXGPXGGPXPRAXXPPXPXP 1206 P P K P PP P P G P P A PP P P Sbjct: 1193 PPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAGLPPVPVPTAKAPPVPAP 1242 Score = 27.9 bits (59), Expect = 4.0 Identities = 13/37 (35%), Positives = 14/37 (37%), Gaps = 3/37 (8%) Frame = +1 Query: 1111 PPARXPXXXPGPLXGPXGGP---XPRAXXPPXPXPPG 1212 PP P P+ P G P P PP P P G Sbjct: 1121 PPVPKPSVAAPPVPVPSGAPPVPKPSVAAPPVPAPSG 1157 Score = 27.5 bits (58), Expect = 5.3 Identities = 16/50 (32%), Positives = 17/50 (34%) Frame = +1 Query: 1057 PXGGXPGXXKXXXTEPEXPPARXPXXXPGPLXGPXGGPXPRAXXPPXPXP 1206 P G P K P PP P P G P P + PP P P Sbjct: 1174 PSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVP-PPSTAPPVPTP 1222 Score = 27.1 bits (57), Expect = 7.0 Identities = 12/35 (34%), Positives = 13/35 (37%), Gaps = 3/35 (8%) Frame = +1 Query: 1111 PPARXPXXXPGPLXGPXGG---PXPRAXXPPXPXP 1206 PP P P+ P G P P PP P P Sbjct: 1140 PPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAP 1174 >SPBC26H8.04c |||DEP domain|Schizosaccharomyces pombe|chr 2|||Manual Length = 1496 Score = 28.7 bits (61), Expect = 2.3 Identities = 16/60 (26%), Positives = 27/60 (45%) Frame = +2 Query: 173 SKYLYN*KSN*NWYFNFTINCNKTVHSVVKK*NIHLSWGLLNCIY*SNQLEIDKNNHELK 352 S +L N K + W + F I CN + +S + ++ W + +Q E HEL+ Sbjct: 465 SSFLNNLKEDTEWLYTFPIGCNISFYSTTEMRSLRSPW---ENLMREHQFEPTAKMHELQ 521 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 28.7 bits (61), Expect = 2.3 Identities = 18/59 (30%), Positives = 20/59 (33%), Gaps = 3/59 (5%) Frame = +1 Query: 1042 PXRNXPXGGX---PGXXKXXXTEPEXPPARXPXXXPGPLXGPXGGPXPRAXXPPXPXPP 1209 P R+ P G P + P PP P P P G R PP P PP Sbjct: 365 PPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRS-APALPPLGNAS-RTSTPPVPTPP 421 Score = 26.6 bits (56), Expect = 9.3 Identities = 15/50 (30%), Positives = 16/50 (32%) Frame = +1 Query: 1057 PXGGXPGXXKXXXTEPEXPPARXPXXXPGPLXGPXGGPXPRAXXPPXPXP 1206 P P P PP+ P P P P P P A P P P Sbjct: 432 PPSAPPSLPMGAPAAPPLPPS-APIAPPLPAGMPAAPPLPPAAPAPPPAP 480 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 28.3 bits (60), Expect = 3.0 Identities = 12/37 (32%), Positives = 14/37 (37%) Frame = +1 Query: 1111 PPARXPXXXPGPLXGPXGGPXPRAXXPPXPXPPGXXG 1221 PP R P + P P P + PP PP G Sbjct: 1690 PPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAG 1726 >SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma subunit|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 27.1 bits (57), Expect = 7.0 Identities = 12/27 (44%), Positives = 17/27 (62%), Gaps = 1/27 (3%) Frame = +1 Query: 445 KDEVG-VRCQKLFQDFLEEFKEDNELK 522 KD+ G +RCQ +F + F E N+LK Sbjct: 283 KDDDGRIRCQPIFSRIISLFAEHNDLK 309 >SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Schizosaccharomyces pombe|chr 1|||Manual Length = 1995 Score = 27.1 bits (57), Expect = 7.0 Identities = 14/41 (34%), Positives = 14/41 (34%) Frame = +2 Query: 1118 PGTPXPXPGPXXGRXGALXPGQXXPPXXPPRGQXGAKXKPG 1240 P P P P G A P PP PP G PG Sbjct: 1882 PPPPPPMALPKAGPPSA-APTSALPPAGPPAGATAISGNPG 1921 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 4,445,351 Number of Sequences: 5004 Number of extensions: 72777 Number of successful extensions: 231 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 187 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 222 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 878103082 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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