BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030623sawa_A07_e49_01.seq (1453 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC977.08 |||short chain dehydrogenase |Schizosaccharomyces pom... 49 1e-06 SPBC1348.09 |||short chain dehydrogenase |Schizosaccharomyces po... 49 1e-06 SPCC162.03 |||short chain dehydrogenase |Schizosaccharomyces pom... 48 2e-06 SPAC922.06 |||short chain dehydrogenase|Schizosaccharomyces pomb... 46 1e-05 SPAC521.03 |||short chain dehydrogenase |Schizosaccharomyces pom... 38 0.003 SPCC736.13 |||short chain dehydrogenase|Schizosaccharomyces pomb... 33 0.099 SPAC31A2.11c |cuf1||Cu metalloregulatory transcription factor Cu... 29 1.2 SPAC22A12.17c |||short chain dehydrogenase |Schizosaccharomyces ... 29 2.1 SPAC4H3.08 |||short chain dehydrogenase |Schizosaccharomyces pom... 28 2.8 SPAC8E11.10 |||sorbose reductase |Schizosaccharomyces pombe|chr ... 28 3.7 SPBC1703.05 |||protein kinase, RIO family|Schizosaccharomyces po... 27 4.9 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 27 8.6 >SPAC977.08 |||short chain dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 236 Score = 49.2 bits (112), Expect = 1e-06 Identities = 32/114 (28%), Positives = 55/114 (48%), Gaps = 5/114 (4%) Frame = +3 Query: 219 KNVLFCRLDVTDYPQFEEAFVMTIEEFNRVDILINNAGFM----NDRLWELEVDVNLNGV 386 +NVL +LDV ++ +++ I F R+D+L+NNAG+ + E ++ N Sbjct: 14 ENVLVTQLDVNNFSSIKKSVEKAISHFGRIDVLLNNAGYSVYSPLESTTEEQIHNIFNTN 73 Query: 387 IRGTLLAYRFMGK-DRGXHGGIIVNTASTAFAKPQVSTPIYTATNYAIVGLTRA 545 + G L + + R H G+I+N +S +Y T YAI G++ A Sbjct: 74 VFGALEVIKAITPIFRSQHNGMIINVSSIGGKMTFPLGCLYYGTKYAIEGISEA 127 >SPBC1348.09 |||short chain dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 236 Score = 49.2 bits (112), Expect = 1e-06 Identities = 32/114 (28%), Positives = 55/114 (48%), Gaps = 5/114 (4%) Frame = +3 Query: 219 KNVLFCRLDVTDYPQFEEAFVMTIEEFNRVDILINNAGFM----NDRLWELEVDVNLNGV 386 +NVL +LDV ++ +++ I F R+D+L+NNAG+ + E ++ N Sbjct: 14 ENVLVTQLDVNNFSSIKKSVEKAISHFGRIDVLLNNAGYSVYSPLESTTEEQIHNIFNTN 73 Query: 387 IRGTLLAYRFMGK-DRGXHGGIIVNTASTAFAKPQVSTPIYTATNYAIVGLTRA 545 + G L + + R H G+I+N +S +Y T YAI G++ A Sbjct: 74 VFGALEVIKAITPIFRSQHNGMIINVSSIGGKMTFPLGCLYYGTKYAIEGISEA 127 >SPCC162.03 |||short chain dehydrogenase |Schizosaccharomyces pombe|chr 3|||Manual Length = 292 Score = 48.4 bits (110), Expect = 2e-06 Identities = 39/114 (34%), Positives = 57/114 (50%), Gaps = 8/114 (7%) Frame = +3 Query: 225 VLFCRLDVTDYPQFEEAFVMTIEEFNRVDILINNAG------FMNDRLWELEVDVNLN-- 380 +L +LDVTD E AF F VDI+INNAG F + + E+ +N+N Sbjct: 49 LLKLKLDVTDVKSVETAFKDAKRRFGNVDIVINNAGYGLVGEFESYNIEEMHRQMNVNFW 108 Query: 381 GVIRGTLLAYRFMGKDRGXHGGIIVNTASTAFAKPQVSTPIYTATNYAIVGLTR 542 GV T A M ++ G GG I+ +S A P +Y A+ +A+ GL++ Sbjct: 109 GVAYITKEALNLM-RESG-KGGRILQISSVAGYYPSPCLSMYNASKFAVEGLSQ 160 >SPAC922.06 |||short chain dehydrogenase|Schizosaccharomyces pombe|chr 1|||Manual Length = 258 Score = 46.4 bits (105), Expect = 1e-05 Identities = 35/117 (29%), Positives = 55/117 (47%), Gaps = 6/117 (5%) Frame = +3 Query: 228 LFCRLDVTDYPQFEEAFVMTIEEFNRVDILINNAGFMNDRLWEL----EVDVNLNGVIRG 395 L + DV+ Q E A I+ +D+LINNAG +D +E D +++ V+RG Sbjct: 48 LALQADVSKADQIETAIEKVIQTLGPIDVLINNAGLADDTPFEQLSHESWDHDVSLVLRG 107 Query: 396 TLLAYRFM--GKDRGXHGGIIVNTASTAFAKPQVSTPIYTATNYAIVGLTRAYGDQY 560 L R++ + GG IVN S + +P Y+A + LT+A +Y Sbjct: 108 NYLTQRYVIPHMAKQGKGGSIVNIGSVN-GHIYLGSPAYSAAKAGLENLTKALAVRY 163 >SPAC521.03 |||short chain dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 259 Score = 38.3 bits (85), Expect = 0.003 Identities = 34/118 (28%), Positives = 54/118 (45%), Gaps = 10/118 (8%) Frame = +3 Query: 222 NVLFCRLDVTDYPQFEEAFVMTIEEFNRVDILINNAGFM--NDRLWELEVD-------VN 374 +VL +LDV+D +EF +D+LINNAG D++ +L +D N Sbjct: 58 SVLPLKLDVSDLKSIPGVIESLPKEFADIDVLINNAGLALGTDKVIDLNIDDAVTMITTN 117 Query: 375 LNGVIRGTLLAYR-FMGKDRGXHGGIIVNTASTAFAKPQVSTPIYTATNYAIVGLTRA 545 + G++ T F K++G I+N S A + V +Y +T A+ T A Sbjct: 118 VLGMMAMTRAVLPIFYSKNKGD----ILNVGSIAGRESYVGGSVYCSTKSALAQFTSA 171 >SPCC736.13 |||short chain dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual Length = 339 Score = 33.1 bits (72), Expect = 0.099 Identities = 15/39 (38%), Positives = 23/39 (58%) Frame = +3 Query: 225 VLFCRLDVTDYPQFEEAFVMTIEEFNRVDILINNAGFMN 341 + F RLD+ D+ +A I + ++ IL+NNAG MN Sbjct: 95 IRFLRLDLLDFESVYQAAESFIAKEEKLHILVNNAGIMN 133 >SPAC31A2.11c |cuf1||Cu metalloregulatory transcription factor Cuf1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 411 Score = 29.5 bits (63), Expect = 1.2 Identities = 13/32 (40%), Positives = 16/32 (50%) Frame = -3 Query: 479 CECCGSSVHDDTAMTAAVLSHESIR*ESTSDH 384 CEC G H + A T A L+H S + T H Sbjct: 334 CECLGCLTHPNNATTLAALNHISALEKETISH 365 >SPAC22A12.17c |||short chain dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 261 Score = 28.7 bits (61), Expect = 2.1 Identities = 20/59 (33%), Positives = 30/59 (50%), Gaps = 8/59 (13%) Frame = +3 Query: 234 CRLDVTDYPQFEEAFVMTIEEFNRVDILINNAGFMN-----DRLWE---LEVDVNLNGV 386 C+ DVT + E AF + F+ +DI++ N G D +E E++VNL GV Sbjct: 75 CKCDVTIPKEVEHAFAEIQKVFDTIDIVVPNNGICTGKSAIDMTYEEFANEINVNLLGV 133 >SPAC4H3.08 |||short chain dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 286 Score = 28.3 bits (60), Expect = 2.8 Identities = 25/100 (25%), Positives = 40/100 (40%), Gaps = 9/100 (9%) Frame = +3 Query: 288 IEEFNRVDILINNAGF---------MNDRLWELEVDVNLNGVIRGTLLAYRFMGKDRGXH 440 +++ +D+L+NN + ++D W+L N+ T A M Sbjct: 116 LKKLGWIDVLVNNIAYQQVAQSIEDIDDEQWDLTFKTNIFSFFWVTKAAISHMKS----- 170 Query: 441 GGIIVNTASTAFAKPQVSTPIYTATNYAIVGLTRAYGDQY 560 G IVN +S + YT+T AI TR +QY Sbjct: 171 GSSIVNCSSINAYVGRPDLLDYTSTKGAITAFTRGLSNQY 210 >SPAC8E11.10 |||sorbose reductase |Schizosaccharomyces pombe|chr 1|||Manual Length = 255 Score = 27.9 bits (59), Expect = 3.7 Identities = 29/96 (30%), Positives = 39/96 (40%), Gaps = 10/96 (10%) Frame = +3 Query: 288 IEEFN-RVDILINNAGFM---------NDRLWELEVDVNLNGVIRGTLLAYRFMGKDRGX 437 +EE R+D++I NAG N+ +W V +NLNG T A K +G Sbjct: 82 VEELGGRLDVMIANAGIAIPHLSLEDKNEDIWTKVVGINLNGAYY-TAQAAGHHFKKQGK 140 Query: 438 HGGIIVNTASTAFAKPQVSTPIYTATNYAIVGLTRA 545 I + S A Y AT A+ L RA Sbjct: 141 GSLIFTASMSGHIANWPQQWASYHATKAAVKHLARA 176 >SPBC1703.05 |||protein kinase, RIO family|Schizosaccharomyces pombe|chr 2|||Manual Length = 336 Score = 27.5 bits (58), Expect = 4.9 Identities = 14/37 (37%), Positives = 22/37 (59%) Frame = +3 Query: 252 DYPQFEEAFVMTIEEFNRVDILINNAGFMNDRLWELE 362 D P FE+ + +E+ N +DI+I +GF + ELE Sbjct: 274 DVPNFED--ISKMEKENNLDIMIEASGFNKKQSKELE 308 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 26.6 bits (56), Expect = 8.6 Identities = 16/39 (41%), Positives = 25/39 (64%), Gaps = 2/39 (5%) Frame = -3 Query: 230 QNVLPTVFGY-PVHQLFVR-QRQNRYRILIPWLLVLKNS 120 QN+L +V G PV +L +R+NR ++L+P+L L S Sbjct: 827 QNLLMSVVGQVPVDELVEEVERRNRLKLLLPYLESLLQS 865 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,182,303 Number of Sequences: 5004 Number of extensions: 53186 Number of successful extensions: 115 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 114 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 115 length of database: 2,362,478 effective HSP length: 75 effective length of database: 1,987,178 effective search space used: 810768624 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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