Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030623sawa_A04_e25_02.seq
(1469 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 142 8e-35
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 67 5e-12
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 64 4e-11
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 49 1e-06
SPCC11E10.08 |rik1||silencing protein Rik1|Schizosaccharomyces p... 27 6.5
SPAC13C5.03 |tht1||nuclear membrane protein involved in karyogam... 27 6.5
SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc... 27 8.6
SPBC609.05 |pob3||FACT complex component Pob3|Schizosaccharomyce... 27 8.6
SPAC222.13c |||6-phosphofructo-2-kinase |Schizosaccharomyces pom... 27 8.6
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 142 bits (345), Expect = 8e-35
Identities = 64/89 (71%), Positives = 79/89 (88%), Gaps = 1/89 (1%)
Frame = +2
Query: 83 IPPKGLKMSSTFIGNTTAIQELFKRISEQFSAMFRRKAFLHWYTGEGMDEMEFNEAESNV 262
+PPK LKMS+TFIGN+T+IQE+F+R+ +QFSAMFRRKAFLHWYTGEGMDEMEF EAESN+
Sbjct: 356 VPPKDLKMSATFIGNSTSIQEIFRRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNM 415
Query: 263 NDLVSEYQQYQDA-TAEDDTEFDQEDMEE 346
NDLVSEYQQYQ+A E D +++ E+ +E
Sbjct: 416 NDLVSEYQQYQEAGIDEGDEDYEIEEEKE 444
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 67.3 bits (157), Expect = 5e-12
Identities = 29/73 (39%), Positives = 48/73 (65%), Gaps = 1/73 (1%)
Frame = +2
Query: 119 IGNTTAIQELFKRISEQFSAMFRRKAFLHWYTGEGMDEMEFNEAESNVNDLVSEYQQY-Q 295
+ NTT+I E + R+ +F M+ ++AF+HWY GEGM+E EF+EA ++ L +Y++ Q
Sbjct: 382 LSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEEVGQ 441
Query: 296 DATAEDDTEFDQE 334
D+ + E D+E
Sbjct: 442 DSMDNEMYEADEE 454
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 64.5 bits (150), Expect = 4e-11
Identities = 26/66 (39%), Positives = 43/66 (65%)
Frame = +2
Query: 119 IGNTTAIQELFKRISEQFSAMFRRKAFLHWYTGEGMDEMEFNEAESNVNDLVSEYQQYQD 298
+ NTT+I E + R+ +F M+ ++AF+HWY GEGM+E EF+EA ++ L +Y++
Sbjct: 378 LSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEEVGQ 437
Query: 299 ATAEDD 316
+ E D
Sbjct: 438 DSMEVD 443
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 49.2 bits (112), Expect = 1e-06
Identities = 25/69 (36%), Positives = 41/69 (59%), Gaps = 3/69 (4%)
Frame = +2
Query: 101 KMSSTFIGNTTAIQELFKRISEQFSAMFRRKAFLHWYTGEGMDEMEFNEAESN---VNDL 271
++S + N T+I LFKR +Q+ + +R AFL Y E + E + NE +S+ V DL
Sbjct: 372 RVSGLMLANHTSIASLFKRTLDQYDRLRKRNAFLEQYKKEAIFEDDLNEFDSSRDVVADL 431
Query: 272 VSEYQQYQD 298
++EY+ +D
Sbjct: 432 INEYEACED 440
>SPCC11E10.08 |rik1||silencing protein Rik1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1040
Score = 27.1 bits (57), Expect = 6.5
Identities = 11/25 (44%), Positives = 19/25 (76%)
Frame = +2
Query: 368 D*IGSIPPIYEILLLKNMVNKYFIV 442
D I ++ PI+++L+LKN + K F+V
Sbjct: 337 DSIPNLGPIHDLLVLKNDIEKSFLV 361
>SPAC13C5.03 |tht1||nuclear membrane protein involved in karyogamy
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 543
Score = 27.1 bits (57), Expect = 6.5
Identities = 12/46 (26%), Positives = 23/46 (50%)
Frame = -1
Query: 770 LYKSDS*LYFSFLFIRKKQTFDYNNTIIVFYYYECFNFNQLIALWG 633
L+ + S L+F L I ++ + + I+F + CF + L+G
Sbjct: 382 LFATLSSLFFKVLKIHRRPIIVFGSLSIIFIHIYCFKITSWVNLYG 427
>SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein
Ubr1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1958
Score = 26.6 bits (56), Expect = 8.6
Identities = 19/59 (32%), Positives = 29/59 (49%)
Frame = +2
Query: 119 IGNTTAIQELFKRISEQFSAMFRRKAFLHWYTGEGMDEMEFNEAESNVNDLVSEYQQYQ 295
+ N + L+K + E+FS +F RK L WY G+ E + N+N SE + Q
Sbjct: 1703 LNNPHLLFTLYKLL-ERFSLIFLRKCALLWYCRYGVS----FETQPNLNFQNSELSRLQ 1756
>SPBC609.05 |pob3||FACT complex component Pob3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 512
Score = 26.6 bits (56), Expect = 8.6
Identities = 15/35 (42%), Positives = 20/35 (57%), Gaps = 2/35 (5%)
Frame = +2
Query: 263 NDLVSEYQQ--YQDATAEDDTEFDQEDMEELAQDE 361
NDL E QQ A ++D E D+E E L++DE
Sbjct: 445 NDLADETQQTLLTSALDDEDEEGDEEMEEALSEDE 479
>SPAC222.13c |||6-phosphofructo-2-kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 592
Score = 26.6 bits (56), Expect = 8.6
Identities = 11/37 (29%), Positives = 21/37 (56%)
Frame = +3
Query: 216 ARAWTRWNSTRRRAMSMIWFRNTSSTRTRPLKMTRNL 326
A ++ W+S R+RA+ I F N S + +++ +L
Sbjct: 424 ASSFNVWSSVRKRAIETIEFFNPDSYNVKKIRLLNDL 460
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,647,338
Number of Sequences: 5004
Number of extensions: 67772
Number of successful extensions: 228
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 213
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 227
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 818637862
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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