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Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000065-TA|BGIBMGA000065-PA|IPR001216|Cysteine
synthase/cystathionine beta-synthase P-phosphate-binding site,
IPR001926|Pyridoxal-5'-phosphate-dependent enzyme, beta subunit
         (440 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_2393| Best HMM Match : No HMM Matches (HMM E-Value=.)              318   4e-87
SB_32365| Best HMM Match : PALP (HMM E-Value=0.064)                   114   1e-25
SB_18116| Best HMM Match : PALP (HMM E-Value=0)                       114   1e-25
SB_51924| Best HMM Match : PALP (HMM E-Value=3.1e-05)                  38   0.021
SB_2970| Best HMM Match : No HMM Matches (HMM E-Value=.)               37   0.028
SB_44496| Best HMM Match : No HMM Matches (HMM E-Value=.)              35   0.11 
SB_43519| Best HMM Match : Pre-SET (HMM E-Value=0.37)                  31   1.4  
SB_38968| Best HMM Match : RNA_POL_M_15KD (HMM E-Value=0.78)           31   2.5  
SB_2329| Best HMM Match : No HMM Matches (HMM E-Value=.)               31   2.5  
SB_45280| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   9.9  

>SB_2393| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 384

 Score =  318 bits (782), Expect = 4e-87
 Identities = 162/306 (52%), Positives = 206/306 (67%), Gaps = 8/306 (2%)

Query: 15  NILETIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKDRISYTMVLEAQKSGIAKS 74
           NIL+ IGDTPLVR+N I K YGL+CE+ AKCEF N GGS+KDRI   M+ EA++ G  K 
Sbjct: 3   NILQKIGDTPLVRINNIGKTYGLKCELLAKCEFFNAGGSVKDRIGLRMIEEAEREGRLKP 62

Query: 75  GTRFIEPTSGNTGIGVALATAVCGFGCTIVTPDKNSDEKMSTISLLGAEVVQTPAMAPWE 134
           G   IEPTSGNTGIG+ALA AV G+ C IV P+K S+EK+  +  LGAE+V+TP  A ++
Sbjct: 63  GDTLIEPTSGNTGIGLALAAAVKGYRCIIVMPEKMSNEKVDVLRALGAEIVRTPTEAKFD 122

Query: 135 SSEHFLSVAKRRLLEDPNAISCDQYKNDVNPRTHYEYTAEEILALVP-DVDMIXXXXXXX 193
           S E  + VA+R   E  N+   DQY+N  NP  HY+ TA+EIL      VDM+       
Sbjct: 123 SPESHIGVAQRLNAEIANSHILDQYRNPGNPLAHYDSTADEILQQCDGKVDMVVLGAGTG 182

Query: 194 XXXXXXAQKVKEKCPKCIIVTVDPQGSLI-----FGEGPADLYFVEGIGGDFVPDVLDKS 248
                 A+K+KEKCP   IV VDP+GS++       E    +Y VEGIG DFVP VLD+S
Sbjct: 183 GTITGIARKLKEKCPGVTIVGVDPKGSILAEPESLNESDVSMYHVEGIGYDFVPTVLDRS 242

Query: 249 VIDKVEKPNDYDAFNMSREIIKKEGLLCGGSSGAAMISAIRAAKKLNFGAGKRVVVILPD 308
           V+DK  K  D  +F MSR +I++EGLLCGGSSG+AM++A+ AAK LN   G+R VVILPD
Sbjct: 243 VVDKWYKSEDKTSFTMSRRLIREEGLLCGGSSGSAMVAAVEAAKSLN--EGQRCVVILPD 300

Query: 309 GIRNYM 314
            +RNY+
Sbjct: 301 SVRNYI 306


>SB_32365| Best HMM Match : PALP (HMM E-Value=0.064)
          Length = 129

 Score =  114 bits (275), Expect = 1e-25
 Identities = 58/126 (46%), Positives = 80/126 (63%)

Query: 33  KDYGLECEMFAKCEFVNPGGSIKDRISYTMVLEAQKSGIAKSGTRFIEPTSGNTGIGVAL 92
           K  GL+CE+ AKCEF+NP GS+KDRI   M+ +A++    K G   IEPTSGNTGI +AL
Sbjct: 4   KRCGLKCEVVAKCEFMNPVGSLKDRIGVRMIEDAERDNHIKPGDTLIEPTSGNTGIALAL 63

Query: 93  ATAVCGFGCTIVTPDKNSDEKMSTISLLGAEVVQTPAMAPWESSEHFLSVAKRRLLEDPN 152
           A+AV G+ C IV  +K S +K  T   LG   V+ PA A ++S E  + +A++   E PN
Sbjct: 64  ASAVKGYRCVIVMSEKMSSDKADTQKALGGICVRAPAEAKFDSPESTIRLAQQLQKEIPN 123

Query: 153 AISCDQ 158
           +   +Q
Sbjct: 124 SYVLNQ 129


>SB_18116| Best HMM Match : PALP (HMM E-Value=0)
          Length = 470

 Score =  114 bits (275), Expect = 1e-25
 Identities = 58/126 (46%), Positives = 80/126 (63%)

Query: 33  KDYGLECEMFAKCEFVNPGGSIKDRISYTMVLEAQKSGIAKSGTRFIEPTSGNTGIGVAL 92
           K  GL+CE+ AKCEF+NP GS+KDRI   M+ +A++    K G   IEPTSGNTGI +AL
Sbjct: 4   KRCGLKCEVVAKCEFMNPVGSLKDRIGVRMIEDAERDNHIKPGDTLIEPTSGNTGIALAL 63

Query: 93  ATAVCGFGCTIVTPDKNSDEKMSTISLLGAEVVQTPAMAPWESSEHFLSVAKRRLLEDPN 152
           A+AV G+ C IV  +K S +K  T   LG   V+ PA A ++S E  + +A++   E PN
Sbjct: 64  ASAVKGYRCVIVMSEKMSSDKADTQKALGGICVRAPAEAKFDSPESTIRLAQQLQKEIPN 123

Query: 153 AISCDQ 158
           +   +Q
Sbjct: 124 SYVLNQ 129



 Score =   99 bits (238), Expect = 4e-21
 Identities = 86/292 (29%), Positives = 142/292 (48%), Gaps = 55/292 (18%)

Query: 183 VDMIXXXXXXXXXXXXXAQKVKEKCPKCIIVTVDPQGSLIFGEGPADL---------YFV 233
           +DM+             A+K++EK P   IV VDP GS++    P +L         Y V
Sbjct: 192 LDMLVIGTGTGGTLTGVARKIREKLPHVKIVAVDPYGSVL--AVPEELNKTEPGFKGYNV 249

Query: 234 EGIGGDFVPDVLDKSVIDKVEKPNDYDAFNMSREIIKKEGLLCGGSSGAAMISAIRAAKK 293
           EGIG  +VP VLD+ ++D+ +K  D +AF  ++ + K+EGLL GGSSG+ M +A+RAA+ 
Sbjct: 250 EGIGKPYVPVVLDRDLVDEWQKIGDKEAFYFAKRLSKEEGLLVGGSSGSVMAAAVRAAQD 309

Query: 294 LNFGAGKRVVVILPDGIRNYM--------------TKFVCDQWMEAHLFK-------EPP 332
           L+    +R VV+  D IRNYM              +KFV D+W+E    +       +P 
Sbjct: 310 LD--ENQRCVVVFADSIRNYMYVPNRIVAWQKKIWSKFVSDEWLEERNLQHDDINDNDPQ 367

Query: 333 EREFLFFKGVLT--KDSLRSEATN-----PKKAGRIDFNEPVNRHLIKTYYKIVENRGNP 385
               L  K +L    + +    T+      + AGR+D +  + + + K + ++  +    
Sbjct: 368 HCSNLTVKDLLAFFSNGILGIVTHGNLLASEAAGRVDMSSTLEKLIYKKFDQVSRDTSLE 427

Query: 386 TVGLASRILDIAPFVIIVEAYYENSNKKQFKGYIPKGIVTTEHVMEYILEHQ 437
            V   SR LD   FV++        + ++F       +VT  H++ +I+E +
Sbjct: 428 KV---SRCLDNDYFVLV-----PGCDSQEF------SVVTRGHLLNFIIERE 465


>SB_51924| Best HMM Match : PALP (HMM E-Value=3.1e-05)
          Length = 407

 Score = 37.5 bits (83), Expect = 0.021
 Identities = 31/118 (26%), Positives = 51/118 (43%), Gaps = 7/118 (5%)

Query: 14  NNILETIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKDRISYTMVLEAQKSGIAK 73
           + I   I  TP++  + + K  G    +F KCE +   G+ K R +   VL   +S    
Sbjct: 106 DRIAPYINSTPVMTSHTLDKMAGRS--LFFKCENLQKAGAFKFRGAMNAVLRLVESSDDP 163

Query: 74  SGTRFIEP-----TSGNTGIGVALATAVCGFGCTIVTPDKNSDEKMSTISLLGAEVVQ 126
           SG++   P     +SGN    ++LA  +      IV P      K + +   GA +V+
Sbjct: 164 SGSKSQAPCVVTHSSGNHAQALSLAAKLMNLKAHIVMPKNAPHVKKAAVIEYGANIVE 221


>SB_2970| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 85

 Score = 37.1 bits (82), Expect = 0.028
 Identities = 20/63 (31%), Positives = 31/63 (49%), Gaps = 1/63 (1%)

Query: 114 MSTISLLGAEVVQTPAMAPWESSEHFLSVAKRRLLEDPNAISCDQYKNDVNPRTHYEYTA 173
           M  +  LGAEV   P + P++   ++   A+R      N +  +Q+ N  N R H+E T 
Sbjct: 1   MDLLKALGAEVRPVPVV-PFDDQMNYNHQARRYAESIENGVWTNQFDNTANRRAHFETTG 59

Query: 174 EEI 176
            EI
Sbjct: 60  PEI 62


>SB_44496| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 27

 Score = 35.1 bits (77), Expect = 0.11
 Identities = 16/26 (61%), Positives = 20/26 (76%)

Query: 88  IGVALATAVCGFGCTIVTPDKNSDEK 113
           IG+ALA AV G+ C IV P+K S+EK
Sbjct: 2   IGLALAAAVKGYRCIIVMPEKMSNEK 27


>SB_43519| Best HMM Match : Pre-SET (HMM E-Value=0.37)
          Length = 829

 Score = 31.5 bits (68), Expect = 1.4
 Identities = 19/74 (25%), Positives = 33/74 (44%), Gaps = 2/74 (2%)

Query: 101 CTIVTPDKNSDEKMSTISLLGAEVVQTPAMAPWESSEHFLSVAKRRLLEDPNAISCDQYK 160
           C +  PD +SD   ST+S+     +      P    EH +   K  ++   +  S D+Y+
Sbjct: 163 CVVFDPDNDSDS--STLSVFLERYMSDGWEGPEPYKEHHVINRKEWVVTSDDGSSEDRYE 220

Query: 161 NDVNPRTHYEYTAE 174
           +DV+      Y +E
Sbjct: 221 DDVSSANELGYASE 234


>SB_38968| Best HMM Match : RNA_POL_M_15KD (HMM E-Value=0.78)
          Length = 152

 Score = 30.7 bits (66), Expect = 2.5
 Identities = 17/47 (36%), Positives = 26/47 (55%)

Query: 131 APWESSEHFLSVAKRRLLEDPNAISCDQYKNDVNPRTHYEYTAEEIL 177
           A +E SE + S+  RR+L + + I CDQ+K+       + Y  EE L
Sbjct: 34  ACYEISEGYGSLQNRRVLGEVSMIFCDQHKSLKKSGATWSYEEEEEL 80


>SB_2329| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1181

 Score = 30.7 bits (66), Expect = 2.5
 Identities = 17/47 (36%), Positives = 26/47 (55%)

Query: 131 APWESSEHFLSVAKRRLLEDPNAISCDQYKNDVNPRTHYEYTAEEIL 177
           A +E SE + S+  RR+L + + I CDQ+K+       + Y  EE L
Sbjct: 912 ACYEISEGYGSLQNRRVLGEVSMIFCDQHKSLKKSGATWSYEEEEEL 958


>SB_45280| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 106

 Score = 28.7 bits (61), Expect = 9.9
 Identities = 19/68 (27%), Positives = 31/68 (45%), Gaps = 5/68 (7%)

Query: 315 TKFVCDQWMEAHLFKEPPEREFLFFKGVLTKDSLRSEAT----NPKKAGRIDFNEPVNR- 369
           T  +C QW   +  K+P + + L  K +L +     + T    NPK A   ++    NR 
Sbjct: 24  TNIICGQWYHKYRKKKPRDAKVLNIKELLEEGEANEKVTESEANPKPAINTEWAHSENRF 83

Query: 370 HLIKTYYK 377
           + +KT  K
Sbjct: 84  NSLKTAEK 91


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.137    0.402 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,658,380
Number of Sequences: 59808
Number of extensions: 597459
Number of successful extensions: 1576
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 1562
Number of HSP's gapped (non-prelim): 11
length of query: 440
length of database: 16,821,457
effective HSP length: 84
effective length of query: 356
effective length of database: 11,797,585
effective search space: 4199940260
effective search space used: 4199940260
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 61 (28.7 bits)

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