GeneModel BLAST Result against arabidopsis

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000066-TA|BGIBMGA000066-PA|IPR001515|Ribosomal protein
L32e
         (134 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g18100.1 68417.m02692 60S ribosomal protein L32 (RPL32A) ribo...   190   4e-49
At5g46430.2 68418.m05716 60S ribosomal protein L32 (RPL32B)           186   3e-48
At5g46430.1 68418.m05715 60S ribosomal protein L32 (RPL32B)           186   3e-48
At1g53920.1 68414.m06138 GDSL-motif lipase/hydrolase family prot...    29   1.4  
At5g51860.1 68418.m06429 MADS-box protein (AGL72) contains Pfam ...    28   2.5  
At4g07670.1 68417.m01203 protease-associated (PA) domain-contain...    27   3.2  
At5g64420.1 68418.m08092 DNA polymerase V family contains Pfam d...    27   4.3  
At3g56790.1 68416.m06316 RNA splicing factor-related contains we...    27   4.3  
At2g34240.1 68415.m04189 hypothetical protein contains Pfam prof...    27   4.3  
At4g35940.1 68417.m05113 expressed protein                             26   7.5  
At1g64625.1 68414.m07326 expressed protein similar to cDNA bHLH ...    26   7.5  
At4g12430.1 68417.m01967 trehalose-6-phosphate phosphatase, puta...    26   9.9  

>At4g18100.1 68417.m02692 60S ribosomal protein L32 (RPL32A)
           ribosomal protein L32, human, PIR1:R5HU32
          Length = 133

 Score =  190 bits (462), Expect = 4e-49
 Identities = 89/134 (66%), Positives = 108/134 (80%), Gaps = 1/134 (0%)

Query: 1   MAIRPVYRPTIVKKRTKRFIRHQSDRYDKLKRNWRKPRGIDNRVRRRFKGQYLMPNIGYG 60
           MA+ P+    +VKKR+ +FIR QSDR   +K +WR+P+GID+RVRR+FKG  LMPN+GYG
Sbjct: 1   MAV-PLLTKKVVKKRSAKFIRPQSDRRITVKESWRRPKGIDSRVRRKFKGVTLMPNVGYG 59

Query: 61  SNKKTRHMLPNGFRKVLVHNVKELEILMMQNRKYCAEIAHGVSSKKRKLIVERAQQLSIR 120
           S+KKTRH LPNGF+K +VHN  ELE+LMM NR YCAEIAH VS+KKRK IVERA QL + 
Sbjct: 60  SDKKTRHYLPNGFKKFVVHNTSELELLMMHNRTYCAEIAHNVSTKKRKAIVERASQLDVV 119

Query: 121 VTNAAARLRSQENE 134
           VTN  ARLRSQE+E
Sbjct: 120 VTNRLARLRSQEDE 133


>At5g46430.2 68418.m05716 60S ribosomal protein L32 (RPL32B)
          Length = 133

 Score =  186 bits (454), Expect = 3e-48
 Identities = 86/134 (64%), Positives = 107/134 (79%), Gaps = 1/134 (0%)

Query: 1   MAIRPVYRPTIVKKRTKRFIRHQSDRYDKLKRNWRKPRGIDNRVRRRFKGQYLMPNIGYG 60
           MA+ P+    +VKKR+ +FIR QSDR   +K +WR+P+GID+RVRR+FKG  LMPN+GYG
Sbjct: 1   MAV-PLLTKKVVKKRSAKFIRPQSDRRITVKESWRRPKGIDSRVRRKFKGVTLMPNVGYG 59

Query: 61  SNKKTRHMLPNGFRKVLVHNVKELEILMMQNRKYCAEIAHGVSSKKRKLIVERAQQLSIR 120
           S+KKTRH LPNGF+K +VHN  ELE+LMM NR YCAEIAH +S+KKRK IVERA QL + 
Sbjct: 60  SDKKTRHYLPNGFKKFIVHNTSELELLMMHNRTYCAEIAHNISTKKRKAIVERASQLDVV 119

Query: 121 VTNAAARLRSQENE 134
           V+N   RLRSQE+E
Sbjct: 120 VSNKLGRLRSQEDE 133


>At5g46430.1 68418.m05715 60S ribosomal protein L32 (RPL32B)
          Length = 133

 Score =  186 bits (454), Expect = 3e-48
 Identities = 86/134 (64%), Positives = 107/134 (79%), Gaps = 1/134 (0%)

Query: 1   MAIRPVYRPTIVKKRTKRFIRHQSDRYDKLKRNWRKPRGIDNRVRRRFKGQYLMPNIGYG 60
           MA+ P+    +VKKR+ +FIR QSDR   +K +WR+P+GID+RVRR+FKG  LMPN+GYG
Sbjct: 1   MAV-PLLTKKVVKKRSAKFIRPQSDRRITVKESWRRPKGIDSRVRRKFKGVTLMPNVGYG 59

Query: 61  SNKKTRHMLPNGFRKVLVHNVKELEILMMQNRKYCAEIAHGVSSKKRKLIVERAQQLSIR 120
           S+KKTRH LPNGF+K +VHN  ELE+LMM NR YCAEIAH +S+KKRK IVERA QL + 
Sbjct: 60  SDKKTRHYLPNGFKKFIVHNTSELELLMMHNRTYCAEIAHNISTKKRKAIVERASQLDVV 119

Query: 121 VTNAAARLRSQENE 134
           V+N   RLRSQE+E
Sbjct: 120 VSNKLGRLRSQEDE 133


>At1g53920.1 68414.m06138 GDSL-motif lipase/hydrolase family protein
           similar to Anther-specific proline-rich proteins
           SP|P40603 SP|P40602 from {Arabidopsis thaliana};
           contains Pfam profile PF00657: GDSL-like
           Lipase/Acylhydrolase
          Length = 385

 Score = 28.7 bits (61), Expect = 1.4
 Identities = 18/52 (34%), Positives = 25/52 (48%), Gaps = 3/52 (5%)

Query: 20  IRHQSDRYDKLKRNWRKPRGIDNRVRRRFKGQYLMPNIGYGSNKKTRHMLPN 71
           +R Q D Y K++R WR   G +   +R  +  YL   I  GSN  +   L N
Sbjct: 146 LRTQLDHYKKVERLWRTNFGKEESKKRISRAVYL---ISIGSNDYSSIFLTN 194


>At5g51860.1 68418.m06429 MADS-box protein (AGL72) contains Pfam
           profile PF00319: SRF-type transcription factor
           (DNA-binding and dimerisation domain);
          Length = 211

 Score = 27.9 bits (59), Expect = 2.5
 Identities = 17/61 (27%), Positives = 29/61 (47%)

Query: 72  GFRKVLVHNVKELEILMMQNRKYCAEIAHGVSSKKRKLIVERAQQLSIRVTNAAARLRSQ 131
           G +K +V  VK++E+L + NRK   +     S K+   I  + ++    V    A+L   
Sbjct: 90  GLKKEMVTMVKKIEVLEVHNRKMMGQSLDSCSVKELSEIATQIEKSLHMVRLRKAKLYED 149

Query: 132 E 132
           E
Sbjct: 150 E 150


>At4g07670.1 68417.m01203 protease-associated (PA) domain-containing
           protein similar to PF02225: PA domain; similar to
           N-acetylated-alpha-linked acidic dipeptidase II
           (NAALADase II) (SP:Q9Y3Q) {Homo sapiens};similar to
           Glutamate carboxypeptidase II (Membrane glutamate
           carboxypeptidase) (mGCP) (N-acetylated-alpha-linked
           acidic dipeptidase I) (NAALADase I)
           (Pteroylpoly-gamma-glutamate
           carboxypeptidase)(Folylpoly-gamma-glutamate
           carboxypeptidase) (FGCP) (Folate hydrolase 1)
           (Prostate-specific membrane antigen homolog) (SP|O77564)
           {Sus scrofa}
          Length = 280

 Score = 27.5 bits (58), Expect = 3.2
 Identities = 11/37 (29%), Positives = 22/37 (59%)

Query: 2   AIRPVYRPTIVKKRTKRFIRHQSDRYDKLKRNWRKPR 38
           A+ P     ++ + +K +++H + R DKL++   KPR
Sbjct: 194 AVDPNSGTAVLMEASKSYLQHIAQRLDKLQKRGWKPR 230


>At5g64420.1 68418.m08092 DNA polymerase V family contains Pfam domain
            PF04931: DNA polymerase V
          Length = 1306

 Score = 27.1 bits (57), Expect = 4.3
 Identities = 14/32 (43%), Positives = 18/32 (56%)

Query: 75   KVLVHNVKELEILMMQNRKYCAEIAHGVSSKK 106
            K LV N+ E ++   Q RK+C  I   VSS K
Sbjct: 1242 KELVGNMPEAKVRRAQVRKFCGRIFQMVSSLK 1273


>At3g56790.1 68416.m06316 RNA splicing factor-related contains weak
           similarity to U4/U6-associated RNA splicing factor [Homo
           sapiens] gi|2853287|gb|AAC09069
          Length = 206

 Score = 27.1 bits (57), Expect = 4.3
 Identities = 16/60 (26%), Positives = 28/60 (46%)

Query: 60  GSNKKTRHMLPNGFRKVLVHNVKELEILMMQNRKYCAEIAHGVSSKKRKLIVERAQQLSI 119
           GS +K R    NG  +    + +  ++   + R Y   IA   +S +    V+R Q+L+I
Sbjct: 32  GSKRKDRSEEENGAARTTNKSSRFEDVARFEKRPYTCSIASDTTSSQILEAVKRFQELAI 91


>At2g34240.1 68415.m04189 hypothetical protein contains Pfam
           profiles PF04780: Protein of unknown function (DUF629),
           PF04781: Protein of unknown function (DUF627)
          Length = 712

 Score = 27.1 bits (57), Expect = 4.3
 Identities = 15/61 (24%), Positives = 32/61 (52%), Gaps = 1/61 (1%)

Query: 16  TKRFIRHQSDRY-DKLKRNWRKPRGIDNRVRRRFKGQYLMPNIGYGSNKKTRHMLPNGFR 74
           +++ I +  ++Y DK+K ++ K   + N +R R+   ++   + Y      RH+L N  +
Sbjct: 216 SRKLIDYVENKYVDKVKEDFGKCVSVANNLRWRYWKCHICSQVYYCFTDCQRHILDNHVQ 275

Query: 75  K 75
           K
Sbjct: 276 K 276


>At4g35940.1 68417.m05113 expressed protein
          Length = 451

 Score = 26.2 bits (55), Expect = 7.5
 Identities = 17/86 (19%), Positives = 35/86 (40%)

Query: 12  VKKRTKRFIRHQSDRYDKLKRNWRKPRGIDNRVRRRFKGQYLMPNIGYGSNKKTRHMLPN 71
           V+++ K+  R +  R DK  +  +K R      + + + +     +G       R    +
Sbjct: 30  VEEKAKKEQRRKDRRSDKKDKKDKKERKEKKEKKEKKRKEREGKEVGSEKRSHKRRRKED 89

Query: 72  GFRKVLVHNVKELEILMMQNRKYCAE 97
           G +  L H +KE E+  ++      E
Sbjct: 90  GAKVDLFHKLKESEVNCLEKSSLTVE 115


>At1g64625.1 68414.m07326 expressed protein similar to cDNA bHLH
           transcription factor (bHLH epsilon gene) GI:32563003
          Length = 527

 Score = 26.2 bits (55), Expect = 7.5
 Identities = 26/112 (23%), Positives = 47/112 (41%), Gaps = 7/112 (6%)

Query: 16  TKRFIRHQSDRYDKLKRNWRKPRGIDNRVRRRFKGQYLMP-----NIGYGSNKKTRHMLP 70
           T R +    D    +  NW+KP     + +R   G+   P      +     K+ R M+P
Sbjct: 323 TSRSLWIDDDERSSIGGNWKKPHEEGVKKKRAKAGESRRPRPKDRQMIQDRIKELRGMIP 382

Query: 71  NGFRKVLVHNVKELEILMMQNRKYCAEIAHGVSSK-KRKLIVERAQQLSIRV 121
           NG  K  +  + +L I  M   +  A+ A  +    + KL+ E+ +  ++ V
Sbjct: 383 NG-AKCSIDTLLDLTIKHMVFMQSLAKYAERLKQPYESKLVKEKERTWALEV 433


>At4g12430.1 68417.m01967 trehalose-6-phosphate phosphatase,
           putative similar to trehalose-6-phosphate phosphatase
           (AtTPPB) [Arabidopsis thaliana] GI:2944180; contains
           Pfam profile PF02358: Trehalose-phosphatase
          Length = 368

 Score = 25.8 bits (54), Expect = 9.9
 Identities = 11/40 (27%), Positives = 23/40 (57%), Gaps = 1/40 (2%)

Query: 75  KVLVHNVKELEILMMQNRKYCAEIAH-GVSSKKRKLIVER 113
           + LV  +K+++   ++N K+CA + +  V  K   +I +R
Sbjct: 212 RTLVEKMKDIKGAKVENHKFCASVHYRNVDEKDWPIIAQR 251


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.323    0.136    0.394 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,982,650
Number of Sequences: 28952
Number of extensions: 119668
Number of successful extensions: 424
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 415
Number of HSP's gapped (non-prelim): 12
length of query: 134
length of database: 12,070,560
effective HSP length: 74
effective length of query: 60
effective length of database: 9,928,112
effective search space: 595686720
effective search space used: 595686720
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 40 (21.5 bits)
S2: 54 (25.8 bits)