Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30082
(859 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_P62495 Cluster: Eukaryotic peptide chain release factor... 153 7e-36
UniRef50_Q9NAX8 Cluster: Eukaryotic peptide chain release factor... 113 4e-24
UniRef50_Q965E7 Cluster: Eukaryotic polypeptide chain release fa... 111 3e-23
UniRef50_Q5CD84 Cluster: Eukaryotic peptide chain release factor... 109 9e-23
UniRef50_Q9NCP1 Cluster: Eukaryotic peptide chain release factor... 96 1e-18
UniRef50_UPI0000D9F481 Cluster: PREDICTED: similar to eukaryotic... 95 2e-18
UniRef50_A2EPZ2 Cluster: Eukaryotic release factor 1, putative; ... 91 2e-17
UniRef50_Q98RP4 Cluster: Eukaryotic release factor 1 homolog; n=... 87 5e-16
UniRef50_Q8SRZ6 Cluster: PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1;... 83 1e-14
UniRef50_UPI0000DB724B Cluster: PREDICTED: similar to eukaryotic... 62 2e-08
UniRef50_Q8TS00 Cluster: Peptide chain release factor subunit 1-... 57 5e-07
UniRef50_Q46FF0 Cluster: Peptide chain release factor, subunit 1... 57 7e-07
UniRef50_A7DMG3 Cluster: ERF1 domain 2 protein; n=1; Candidatus ... 54 6e-06
UniRef50_UPI000150A1F7 Cluster: eRF1 domain 1 family protein; n=... 52 2e-05
UniRef50_Q8U0J4 Cluster: Peptide chain release factor subunit 1;... 51 3e-05
UniRef50_A0RX46 Cluster: Peptide chain release factor 1; n=2; Cr... 47 5e-04
UniRef50_Q9HNF0 Cluster: Peptide chain release factor subunit 1;... 43 0.011
UniRef50_Q74N59 Cluster: NEQ052; n=1; Nanoarchaeum equitans|Rep:... 36 0.99
UniRef50_UPI0000D9EEA4 Cluster: PREDICTED: hypothetical protein,... 36 1.3
UniRef50_Q0I769 Cluster: Transcriptional activator, putative, Ba... 36 1.7
UniRef50_Q58239 Cluster: Peptide chain release factor subunit 1;... 36 1.7
UniRef50_Q479I8 Cluster: Cation efflux protein; n=1; Dechloromon... 35 2.3
UniRef50_Q0QZI1 Cluster: Gp47; n=1; Phage Syn9|Rep: Gp47 - Phage... 35 2.3
UniRef50_A2QDN2 Cluster: Contig An02c0230, complete genome; n=1;... 35 2.3
UniRef50_Q5G7G7 Cluster: Polyprotein 1a; n=3; Mint virus 1|Rep: ... 35 3.0
UniRef50_Q97MW9 Cluster: Uncharacterized conserved protein, YTFE... 33 9.2
UniRef50_Q4P4N5 Cluster: Putative uncharacterized protein; n=1; ... 33 9.2
>UniRef50_P62495 Cluster: Eukaryotic peptide chain release factor
subunit 1; n=137; Eukaryota|Rep: Eukaryotic peptide
chain release factor subunit 1 - Homo sapiens (Human)
Length = 437
Score = 153 bits (370), Expect = 7e-36
Identities = 72/86 (83%), Positives = 80/86 (93%)
Frame = +1
Query: 1 QLFISADRPNVAGLILAGSADFKTELSQSDMFDPRLQAKIIKLVDVSYGGENGFNQAIEL 180
QLFIS D+ NVAGL+LAGSADFKTELSQSDMFD RLQ+K++KLVD+SYGGENGFNQAIEL
Sbjct: 211 QLFISGDKVNVAGLVLAGSADFKTELSQSDMFDQRLQSKVLKLVDISYGGENGFNQAIEL 270
Query: 181 AAESLQNVKFIQEKKLIGRYFDEISR 258
+ E L NVKFIQEKKLIGRYFDEIS+
Sbjct: 271 STEVLSNVKFIQEKKLIGRYFDEISQ 296
Score = 134 bits (325), Expect = 2e-30
Identities = 61/84 (72%), Positives = 70/84 (83%)
Frame = +3
Query: 255 QDTGKYCFGVDDTLRALELGSVETLICWENLDIQRYVLKSHATNQETILHLTPEQEKDKS 434
QDTGKYCFGV+DTL+ALE+G+VE LI +ENLDI RYVL T +E IL+LTPEQEKDKS
Sbjct: 296 QDTGKYCFGVEDTLKALEMGAVEILIVYENLDIMRYVLHCQGTEEEKILYLTPEQEKDKS 355
Query: 435 HFTDKESGVELELVECQPLLEWLA 506
HFTDKE+G E EL+E PLLEW A
Sbjct: 356 HFTDKETGQEHELIESMPLLEWFA 379
Score = 54.0 bits (124), Expect = 5e-06
Identities = 23/26 (88%), Positives = 25/26 (96%)
Frame = +2
Query: 509 NYKSFGATLEIITDKSQEGSQFVRGF 586
NYK FGATLEI+TDKSQEGSQFV+GF
Sbjct: 381 NYKKFGATLEIVTDKSQEGSQFVKGF 406
>UniRef50_Q9NAX8 Cluster: Eukaryotic peptide chain release factor
subunit 1; n=6; Trypanosomatidae|Rep: Eukaryotic peptide
chain release factor subunit 1 - Trypanosoma brucei
brucei
Length = 452
Score = 113 bits (273), Expect = 4e-24
Identities = 51/85 (60%), Positives = 71/85 (83%)
Frame = +1
Query: 1 QLFISADRPNVAGLILAGSADFKTELSQSDMFDPRLQAKIIKLVDVSYGGENGFNQAIEL 180
QLFI+ DRPN+ GL+LAGSA+FK L QSD+FDPRL+A ++K+VDV++ G+ G NQAI+L
Sbjct: 212 QLFITNDRPNIVGLVLAGSAEFKEVLYQSDLFDPRLKAIVVKVVDVAHPGDVGLNQAIDL 271
Query: 181 AAESLQNVKFIQEKKLIGRYFDEIS 255
AA++L VK +QEKKL+ +FD+I+
Sbjct: 272 AADALSGVKLVQEKKLLQGFFDQIA 296
Score = 77.0 bits (181), Expect = 6e-13
Identities = 39/85 (45%), Positives = 58/85 (68%), Gaps = 2/85 (2%)
Frame = +3
Query: 258 DTGKYCFGVDDTLRALELGSVETLICWENLDIQRY-VLKSH-ATNQETILHLTPEQEKDK 431
DT YCFGV DTL+ LE G+VETLI +E+L+I RY V+K+ A ++ET +H+ E+E +
Sbjct: 298 DTQLYCFGVQDTLKCLEAGAVETLIVYEDLNIYRYTVVKNRGADDEETFVHVMSEEEAKR 357
Query: 432 SHFTDKESGVELELVECQPLLEWLA 506
S+ +ESG +E + ++WLA
Sbjct: 358 SNIHMQESGKTRNEIEQEDFVDWLA 382
Score = 45.6 bits (103), Expect = 0.002
Identities = 18/26 (69%), Positives = 23/26 (88%)
Frame = +2
Query: 509 NYKSFGATLEIITDKSQEGSQFVRGF 586
NY+ FG LE+IT++SQEG+QFVRGF
Sbjct: 384 NYRKFGCALELITNRSQEGTQFVRGF 409
>UniRef50_Q965E7 Cluster: Eukaryotic polypeptide chain release
factor 1; n=3; Paramecium tetraurelia|Rep: Eukaryotic
polypeptide chain release factor 1 - Paramecium
tetraurelia
Length = 437
Score = 111 bits (266), Expect = 3e-23
Identities = 49/84 (58%), Positives = 66/84 (78%)
Frame = +1
Query: 7 FISADRPNVAGLILAGSADFKTELSQSDMFDPRLQAKIIKLVDVSYGGENGFNQAIELAA 186
FI+ DRPNV GL+LAGSADFK +L+ S FD RLQ II +VD++YGGE G NQA++L+
Sbjct: 214 FIAEDRPNVKGLVLAGSADFKNDLAGSQFFDKRLQPLIISVVDINYGGEQGLNQAVQLSQ 273
Query: 187 ESLQNVKFIQEKKLIGRYFDEISR 258
ESL VK+I+EK L+G++F+ I +
Sbjct: 274 ESLLEVKYIREKNLVGQFFENIDK 297
Score = 50.0 bits (114), Expect = 8e-05
Identities = 23/85 (27%), Positives = 49/85 (57%)
Frame = +3
Query: 255 QDTGKYCFGVDDTLRALELGSVETLICWENLDIQRYVLKSHATNQETILHLTPEQEKDKS 434
+DTG +GV DT+RA+E +++TL+C + L R +S T Q+ I ++ + +
Sbjct: 297 KDTGLVVYGVQDTMRAVESQTIKTLVCVDTLQYLRLECQSKQTEQKAIKYIKGNEGYEAG 356
Query: 435 HFTDKESGVELELVECQPLLEWLAK 509
++++G + ++ + L+E L++
Sbjct: 357 SLIEEKNGEQFVILVKEDLVEHLSE 381
Score = 34.3 bits (75), Expect = 4.0
Identities = 12/27 (44%), Positives = 20/27 (74%)
Frame = +2
Query: 506 QNYKSFGATLEIITDKSQEGSQFVRGF 586
+ +K +G ++ITD S EG+QF++GF
Sbjct: 381 EKFKDYGLDFQLITDHSVEGNQFMKGF 407
>UniRef50_Q5CD84 Cluster: Eukaryotic peptide chain release factor
subunit 1; n=5; Eukaryota|Rep: Eukaryotic peptide chain
release factor subunit 1 - Loxodes striatus
Length = 436
Score = 109 bits (262), Expect = 9e-23
Identities = 47/89 (52%), Positives = 70/89 (78%)
Frame = +1
Query: 7 FISADRPNVAGLILAGSADFKTELSQSDMFDPRLQAKIIKLVDVSYGGENGFNQAIELAA 186
FI DR NV G++LAG+A+FK EL+ ++ D R++AK++ ++DV+YGGENGFNQAIEL+
Sbjct: 211 FIQNDRVNVLGIVLAGAAEFKNELAANEYLDQRIRAKVVTIIDVNYGGENGFNQAIELSQ 270
Query: 187 ESLQNVKFIQEKKLIGRYFDEISRTQESI 273
LQNVKFI+EK LI + F+E+++ ++
Sbjct: 271 VQLQNVKFIKEKNLITKLFEEVAQNSITV 299
Score = 82.6 bits (195), Expect = 1e-14
Identities = 36/83 (43%), Positives = 58/83 (69%)
Frame = +3
Query: 255 QDTGKYCFGVDDTLRALELGSVETLICWENLDIQRYVLKSHATNQETILHLTPEQEKDKS 434
Q++ C+G+ DT++ALE+G+VETL+ WENL+ + LK+ T +E+ + L+P+Q +K+
Sbjct: 294 QNSITVCYGLTDTMKALEMGAVETLVIWENLEFIWFKLKNPVTKEESTVVLSPQQATEKN 353
Query: 435 HFTDKESGVELELVECQPLLEWL 503
HF D+ + EL +VE L EWL
Sbjct: 354 HFQDEANQCELNIVERFALTEWL 376
Score = 48.0 bits (109), Expect = 3e-04
Identities = 19/26 (73%), Positives = 24/26 (92%)
Frame = +2
Query: 509 NYKSFGATLEIITDKSQEGSQFVRGF 586
NYK++GA LE +TD+SQEGSQFV+GF
Sbjct: 379 NYKNYGARLEFVTDRSQEGSQFVKGF 404
>UniRef50_Q9NCP1 Cluster: Eukaryotic peptide chain release factor
subunit 1; n=2; Giardia intestinalis|Rep: Eukaryotic
peptide chain release factor subunit 1 - Giardia lamblia
(Giardia intestinalis)
Length = 457
Score = 95.9 bits (228), Expect = 1e-18
Identities = 43/85 (50%), Positives = 65/85 (76%)
Frame = +1
Query: 19 DRPNVAGLILAGSADFKTELSQSDMFDPRLQAKIIKLVDVSYGGENGFNQAIELAAESLQ 198
++P V+GLIL GSADFK LS S +FD RLQ+ ++K +D++YGGE GFNQAIE+A ++L+
Sbjct: 227 NKPIVSGLILGGSADFKNVLSTSAIFDQRLQSIVMKQIDINYGGEQGFNQAIEMAGDTLK 286
Query: 199 NVKFIQEKKLIGRYFDEISRTQESI 273
+VK IQE KL+ + + I++ + +
Sbjct: 287 DVKLIQEVKLLTEFTENIAKDTKRV 311
Score = 55.6 bits (128), Expect = 2e-06
Identities = 30/87 (34%), Positives = 50/87 (57%), Gaps = 5/87 (5%)
Frame = +3
Query: 255 QDTGKYCFGVDDTLRALELGSVETLICWENLDIQRYVLK---SHATNQETILHLTPEQEK 425
+DT + CFG+ DT+R LE+ +VE LI W++L R L+ + T+ I +L Q
Sbjct: 306 KDTKRVCFGITDTIRCLEMSAVEKLIVWDDLPYHRVTLQCVINGETSAPVIKYLLKSQMS 365
Query: 426 DKSHFTDKESG--VELELVECQPLLEW 500
+ + + +G V+L+++ Q LLEW
Sbjct: 366 NPKYLREVINGEEVQLDIMGDQLLLEW 392
Score = 40.3 bits (90), Expect = 0.061
Identities = 16/26 (61%), Positives = 22/26 (84%)
Frame = +2
Query: 509 NYKSFGATLEIITDKSQEGSQFVRGF 586
NYK++GA+LE IT++S EG+QF GF
Sbjct: 396 NYKNYGASLEFITNRSAEGTQFCSGF 421
>UniRef50_UPI0000D9F481 Cluster: PREDICTED: similar to eukaryotic
translation termination factor 1; n=2; Macaca
mulatta|Rep: PREDICTED: similar to eukaryotic
translation termination factor 1 - Macaca mulatta
Length = 136
Score = 95.5 bits (227), Expect = 2e-18
Identities = 46/81 (56%), Positives = 60/81 (74%)
Frame = +3
Query: 264 GKYCFGVDDTLRALELGSVETLICWENLDIQRYVLKSHATNQETILHLTPEQEKDKSHFT 443
GKYCFG++D+L+AL++G+VETLI +ENLD + + S ++ LTPEQEKD SHF
Sbjct: 2 GKYCFGIEDSLKALKMGAVETLIVFENLDNE--ICSSLTKHRRGENSLTPEQEKDTSHFI 59
Query: 444 DKESGVELELVECQPLLEWLA 506
DKE+G E EL+E PLLEW A
Sbjct: 60 DKETGWEHELIESIPLLEWFA 80
Score = 48.8 bits (111), Expect = 2e-04
Identities = 21/32 (65%), Positives = 26/32 (81%)
Frame = +2
Query: 509 NYKSFGATLEIITDKSQEGSQFVRGFRKDSAV 604
NYK GATLEI+TDKSQE SQFV+GF + + +
Sbjct: 82 NYKKCGATLEIVTDKSQEMSQFVKGFGRSAGI 113
>UniRef50_A2EPZ2 Cluster: Eukaryotic release factor 1, putative;
n=2; Trichomonas vaginalis|Rep: Eukaryotic release
factor 1, putative - Trichomonas vaginalis G3
Length = 438
Score = 91.5 bits (217), Expect = 2e-17
Identities = 42/76 (55%), Positives = 58/76 (76%)
Frame = +1
Query: 25 PNVAGLILAGSADFKTELSQSDMFDPRLQAKIIKLVDVSYGGENGFNQAIELAAESLQNV 204
PNV GLILAGSADFK L+QSD+FD RL+ I+ + D+ YGGE GFNQ I++AA L +V
Sbjct: 221 PNVTGLILAGSADFKNVLAQSDLFDIRLRNIILGIYDICYGGEEGFNQTIKMAAPLLADV 280
Query: 205 KFIQEKKLIGRYFDEI 252
+ ++E++L+ + FD I
Sbjct: 281 RLVREQELLQKLFDTI 296
Score = 38.7 bits (86), Expect = 0.19
Identities = 20/81 (24%), Positives = 43/81 (53%)
Frame = +3
Query: 264 GKYCFGVDDTLRALELGSVETLICWENLDIQRYVLKSHATNQETILHLTPEQEKDKSHFT 443
G FG+ +T+ A + G++ET+I W+ L++ R ++ + E + + T Q + H
Sbjct: 300 GPCAFGIKETMMAYDSGAIETMILWDELNVYRCTMEKDNGDSE-VEYYTEYQLEKGDHLK 358
Query: 444 DKESGVELELVECQPLLEWLA 506
+ ++ +++ L EW+A
Sbjct: 359 SEYHHLKEKIL----LTEWMA 375
Score = 35.9 bits (79), Expect = 1.3
Identities = 14/25 (56%), Positives = 20/25 (80%)
Frame = +2
Query: 509 NYKSFGATLEIITDKSQEGSQFVRG 583
++K GA LE +TDKS EG+QF++G
Sbjct: 377 HHKEKGAKLEFVTDKSPEGAQFIKG 401
>UniRef50_Q98RP4 Cluster: Eukaryotic release factor 1 homolog; n=1;
Guillardia theta|Rep: Eukaryotic release factor 1
homolog - Guillardia theta (Cryptomonas phi)
Length = 409
Score = 87.0 bits (206), Expect = 5e-16
Identities = 40/87 (45%), Positives = 62/87 (71%), Gaps = 1/87 (1%)
Frame = +1
Query: 1 QLFISADRPN-VAGLILAGSADFKTELSQSDMFDPRLQAKIIKLVDVSYGGENGFNQAIE 177
Q ++S N + GL++AG AD K EL S++F+ +L+ KI+ ++D+SYGGE GFN+AIE
Sbjct: 207 QYYLSDFNKNFIEGLVIAGPADLKNELISSELFNEKLREKILSIIDISYGGEIGFNKAIE 266
Query: 178 LAAESLQNVKFIQEKKLIGRYFDEISR 258
++ L +K I+EKK+I +FDEI +
Sbjct: 267 NSSSVLDQLKCIKEKKIIESFFDEIEK 293
Score = 49.6 bits (113), Expect = 1e-04
Identities = 27/89 (30%), Positives = 48/89 (53%)
Frame = +3
Query: 255 QDTGKYCFGVDDTLRALELGSVETLICWENLDIQRYVLKSHATNQETILHLTPEQEKDKS 434
+DTGKY +G ++T +L G + +I WENL+I+R V TN E I+ E DK+
Sbjct: 293 KDTGKYVYGCEETCNSLTNGFLSKIILWENLEIERMVYIDSFTNLE-IVKFKKINECDKT 351
Query: 435 HFTDKESGVELELVECQPLLEWLAKTTSR 521
D+ + +C +++W+ + ++
Sbjct: 352 ECLDENPKLVFS-AKCN-VIDWVIENKNK 378
>UniRef50_Q8SRZ6 Cluster: PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1;
n=1; Encephalitozoon cuniculi|Rep: PEPTIDE CHAIN RELEASE
FACTOR SUBUNIT 1 - Encephalitozoon cuniculi
Length = 386
Score = 82.6 bits (195), Expect = 1e-14
Identities = 43/84 (51%), Positives = 61/84 (72%)
Frame = +1
Query: 4 LFISADRPNVAGLILAGSADFKTELSQSDMFDPRLQAKIIKLVDVSYGGENGFNQAIELA 183
LF++ NV GLILAG +D K EL+Q + D R+Q +IK VD +YGGE+G NQAIEL
Sbjct: 205 LFLTNCVMNVEGLILAGQSDLKNELAQ--VLDSRIQ--VIKAVDTNYGGESGLNQAIELC 260
Query: 184 AESLQNVKFIQEKKLIGRYFDEIS 255
+ L++VK +EKK++ R+F+EI+
Sbjct: 261 EDVLKDVKLSKEKKILQRFFNEIN 284
Score = 41.1 bits (92), Expect = 0.035
Identities = 16/27 (59%), Positives = 20/27 (74%)
Frame = +2
Query: 506 QNYKSFGATLEIITDKSQEGSQFVRGF 586
+NYKSFG L ++DKS EG QF+ GF
Sbjct: 330 ENYKSFGCILAFVSDKSAEGMQFIEGF 356
Score = 40.3 bits (90), Expect = 0.061
Identities = 15/30 (50%), Positives = 25/30 (83%)
Frame = +3
Query: 258 DTGKYCFGVDDTLRALELGSVETLICWENL 347
++G++CF + DT++ LE+G+VETLI +E L
Sbjct: 286 ESGRFCFTMRDTMQCLEMGAVETLIVYEGL 315
>UniRef50_UPI0000DB724B Cluster: PREDICTED: similar to eukaryotic
release factor 1 CG5605-PA, isoform A; n=1; Apis
mellifera|Rep: PREDICTED: similar to eukaryotic release
factor 1 CG5605-PA, isoform A - Apis mellifera
Length = 265
Score = 61.7 bits (143), Expect = 2e-08
Identities = 31/44 (70%), Positives = 34/44 (77%), Gaps = 3/44 (6%)
Frame = +3
Query: 384 NQETILH-LTPEQEK--DKSHFTDKESGVELELVECQPLLEWLA 506
N +LH T + K DK+HFTDKESGVELELVECQPLLEWLA
Sbjct: 164 NTREVLHKFTVDLPKKHDKTHFTDKESGVELELVECQPLLEWLA 207
Score = 57.2 bits (132), Expect = 5e-07
Identities = 26/26 (100%), Positives = 26/26 (100%)
Frame = +2
Query: 509 NYKSFGATLEIITDKSQEGSQFVRGF 586
NYKSFGATLEIITDKSQEGSQFVRGF
Sbjct: 209 NYKSFGATLEIITDKSQEGSQFVRGF 234
>UniRef50_Q8TS00 Cluster: Peptide chain release factor subunit 1-2;
n=9; Euryarchaeota|Rep: Peptide chain release factor
subunit 1-2 - Methanosarcina acetivorans
Length = 415
Score = 57.2 bits (132), Expect = 5e-07
Identities = 26/92 (28%), Positives = 50/92 (54%)
Frame = +1
Query: 1 QLFISADRPNVAGLILAGSADFKTELSQSDMFDPRLQAKIIKLVDVSYGGENGFNQAIEL 180
+ F+ D + G+++ G + K E ++ LQ K++ L D Y E+GF++ I
Sbjct: 206 EAFLELDPAELKGILIGGHSPTKEEFNEGGFLHYELQKKVLGLFDTGYTDESGFSELINA 265
Query: 181 AAESLQNVKFIQEKKLIGRYFDEISRTQESIA 276
A E+LQ++ +++KK + +F EI+ I+
Sbjct: 266 AEETLQSIDLLKQKKDMEIFFKEIATESGKIS 297
>UniRef50_Q46FF0 Cluster: Peptide chain release factor, subunit 1;
n=2; Methanosarcinaceae|Rep: Peptide chain release
factor, subunit 1 - Methanosarcina barkeri (strain
Fusaro / DSM 804)
Length = 415
Score = 56.8 bits (131), Expect = 7e-07
Identities = 25/83 (30%), Positives = 47/83 (56%)
Frame = +1
Query: 7 FISADRPNVAGLILAGSADFKTELSQSDMFDPRLQAKIIKLVDVSYGGENGFNQAIELAA 186
F+ D ++ G+++ G + K E ++ LQ K++ L D Y E+GF++ I A
Sbjct: 208 FLELDPSDLKGILIGGHSPTKDEFNEGGFLHYELQNKVLGLFDTEYTDESGFSELINAAE 267
Query: 187 ESLQNVKFIQEKKLIGRYFDEIS 255
++LQ + I++KK I +F E++
Sbjct: 268 DTLQGIDLIKQKKDIEMFFKELT 290
>UniRef50_A7DMG3 Cluster: ERF1 domain 2 protein; n=1; Candidatus
Nitrosopumilus maritimus SCM1|Rep: ERF1 domain 2 protein
- Candidatus Nitrosopumilus maritimus SCM1
Length = 426
Score = 53.6 bits (123), Expect = 6e-06
Identities = 25/75 (33%), Positives = 41/75 (54%)
Frame = +1
Query: 31 VAGLILAGSADFKTELSQSDMFDPRLQAKIIKLVDVSYGGENGFNQAIELAAESLQNVKF 210
V GL+++G K + + + RLQ II +D SY G G +A +++ L N +
Sbjct: 223 VKGLVISGPGPTKEDFINGNYLEYRLQNNIIATIDSSYSGSEGIREAFAKSSDILGNFRL 282
Query: 211 IQEKKLIGRYFDEIS 255
++EKKL+ F EI+
Sbjct: 283 VEEKKLVEALFREIN 297
>UniRef50_UPI000150A1F7 Cluster: eRF1 domain 1 family protein; n=1;
Tetrahymena thermophila SB210|Rep: eRF1 domain 1 family
protein - Tetrahymena thermophila SB210
Length = 519
Score = 52.0 bits (119), Expect = 2e-05
Identities = 24/77 (31%), Positives = 44/77 (57%), Gaps = 1/77 (1%)
Frame = +1
Query: 28 NVAGLILAGSADFKTELSQS-DMFDPRLQAKIIKLVDVSYGGENGFNQAIELAAESLQNV 204
N++G++L G +D K ++ + P ++ KI+ DVSY G+ G QA++++ E L+
Sbjct: 284 NLSGIVLCGMSDIKDKVQKELQQLCPCIENKIVASYDVSYSGQAGLKQALQMSTEMLKLD 343
Query: 205 KFIQEKKLIGRYFDEIS 255
+ QE L+ +F S
Sbjct: 344 QLFQEMNLLSDFFANFS 360
Score = 47.6 bits (108), Expect = 4e-04
Identities = 30/94 (31%), Positives = 53/94 (56%), Gaps = 7/94 (7%)
Frame = +3
Query: 258 DTGKYCFGVDDTLRALELGSVETLICWENLDIQRYVLKSHATNQETILHLTPEQEK---- 425
+T K +G + T+RALE G+V+ LI ++ ++QR + + T +ETI +L P Q K
Sbjct: 362 ETSKVVYGGELTVRALEEGNVKKLILCQDSELQRVTVYNSKTQEETIQYLMPSQVKALQD 421
Query: 426 DKSHFTDKESG---VELELVECQPLLEWLAKTTS 518
S +D+E+ +L++ Q + EW+ + S
Sbjct: 422 SISKTSDQEANNKKNQLQVYSQQNINEWIVENIS 455
>UniRef50_Q8U0J4 Cluster: Peptide chain release factor subunit 1;
n=5; Archaea|Rep: Peptide chain release factor subunit 1
- Pyrococcus furiosus
Length = 420
Score = 51.2 bits (117), Expect = 3e-05
Identities = 24/72 (33%), Positives = 40/72 (55%)
Frame = +1
Query: 37 GLILAGSADFKTELSQSDMFDPRLQAKIIKLVDVSYGGENGFNQAIELAAESLQNVKFIQ 216
G+I+ G K E + D L+ KII +VD+SY GE G + + AA+ L++ + I+
Sbjct: 221 GIIVGGPGPTKEEFVEGDYLHHELKKKIIGIVDISYHGEYGLRELVAKAADILRDHEVIR 280
Query: 217 EKKLIGRYFDEI 252
E+ L+ + I
Sbjct: 281 ERNLVNEFLKHI 292
Score = 34.7 bits (76), Expect = 3.0
Identities = 18/56 (32%), Positives = 32/56 (57%)
Frame = +3
Query: 252 LQDTGKYCFGVDDTLRALELGSVETLICWENLDIQRYVLKSHATNQETILHLTPEQ 419
++DTG +G + +ALELG+V+TL+ E D R +K + E + ++ E+
Sbjct: 293 VKDTGLATYGEREVRKALELGAVDTLLISEGYDKVRVHVKCNNCGWEELKTMSEEE 348
>UniRef50_A0RX46 Cluster: Peptide chain release factor 1; n=2;
Crenarchaeota|Rep: Peptide chain release factor 1 -
Cenarchaeum symbiosum
Length = 411
Score = 47.2 bits (107), Expect = 5e-04
Identities = 21/75 (28%), Positives = 38/75 (50%)
Frame = +1
Query: 31 VAGLILAGSADFKTELSQSDMFDPRLQAKIIKLVDVSYGGENGFNQAIELAAESLQNVKF 210
+ GLI++G K E + RLQ +I +D SY G G ++ +A L + +
Sbjct: 210 IKGLIVSGPGPTKEEFLNGSYLEYRLQEMVIATLDTSYSGSEGIRESFAKSASVLSDFRM 269
Query: 211 IQEKKLIGRYFDEIS 255
++E L+ + F E++
Sbjct: 270 VEEGALVEKLFREVN 284
>UniRef50_Q9HNF0 Cluster: Peptide chain release factor subunit 1;
n=7; Euryarchaeota|Rep: Peptide chain release factor
subunit 1 - Halobacterium salinarium (Halobacterium
halobium)
Length = 416
Score = 42.7 bits (96), Expect = 0.011
Identities = 19/78 (24%), Positives = 38/78 (48%)
Frame = +1
Query: 22 RPNVAGLILAGSADFKTELSQSDMFDPRLQAKIIKLVDVSYGGENGFNQAIELAAESLQN 201
R + G+++ G + K E D LQ K++ DVSY E+G + ++ +L
Sbjct: 219 RHEIDGILVGGPSPTKDEFLDGDYLHHELQDKVLGKFDVSYTDESGLSDLVDAGQAALAE 278
Query: 202 VKFIQEKKLIGRYFDEIS 255
+ +K + +F+E++
Sbjct: 279 ADLMDDKSDMEEFFEELN 296
>UniRef50_Q74N59 Cluster: NEQ052; n=1; Nanoarchaeum equitans|Rep:
NEQ052 - Nanoarchaeum equitans
Length = 358
Score = 36.3 bits (80), Expect = 0.99
Identities = 21/85 (24%), Positives = 43/85 (50%)
Frame = +1
Query: 22 RPNVAGLILAGSADFKTELSQSDMFDPRLQAKIIKLVDVSYGGENGFNQAIELAAESLQN 201
+ + G+I+ GS K + + + + KII +VD Y G +A+ A + L+
Sbjct: 208 KDKIQGIIIGGSGFAKHDFIEYLPY--YYKDKIIAIVDTGYSDAYGLREALYRAEDKLKE 265
Query: 202 VKFIQEKKLIGRYFDEISRTQESIA 276
++ +E+K++ F I+ ++ IA
Sbjct: 266 LEIEKERKILSDLFKAIALGRKDIA 290
>UniRef50_UPI0000D9EEA4 Cluster: PREDICTED: hypothetical protein,
partial; n=1; Macaca mulatta|Rep: PREDICTED:
hypothetical protein, partial - Macaca mulatta
Length = 213
Score = 35.9 bits (79), Expect = 1.3
Identities = 19/67 (28%), Positives = 30/67 (44%), Gaps = 1/67 (1%)
Frame = +1
Query: 286 MIHCALSNSDPSRR*-SAGRTSTYKDTY*SRMPPTRKQFST*RLNKRRTNHISPTKRAEW 462
+ HC+ S++ P R S R + + Y SR PP + FS R + + A W
Sbjct: 75 LAHCSASSTSPGGRVTSVLREAARQCAYGSRQPPRARLFSVRGRLGRNYSSQEDAREAAW 134
Query: 463 SWSLWSA 483
W+ W +
Sbjct: 135 KWAAWKS 141
>UniRef50_Q0I769 Cluster: Transcriptional activator, putative, Baf
family protein; n=2; Synechococcus|Rep: Transcriptional
activator, putative, Baf family protein - Synechococcus
sp. (strain CC9311)
Length = 232
Score = 35.5 bits (78), Expect = 1.7
Identities = 16/54 (29%), Positives = 28/54 (51%)
Frame = -1
Query: 730 PFPSKCLPSLNKSVELSVIRLVEFPRSVEAASDWKVQLCNGGDRRILPKAANEL 569
PFP + ++ + S++ L+ R A S W + LC G ++LPK ++L
Sbjct: 162 PFPFETQAAMRSGAQQSLVGLI---REAHAQSPWPIWLCGGDSPQLLPKLQDQL 212
>UniRef50_Q58239 Cluster: Peptide chain release factor subunit 1;
n=12; Euryarchaeota|Rep: Peptide chain release factor
subunit 1 - Methanococcus jannaschii
Length = 421
Score = 35.5 bits (78), Expect = 1.7
Identities = 28/92 (30%), Positives = 51/92 (55%), Gaps = 7/92 (7%)
Frame = +3
Query: 255 QDTGKYCFGVDDTLRALELGSVETLICWENLDIQRYVLKSHATN-----QETI--LHLTP 413
+D G C+G + L AL +G+V+TLI E ++++Y +K N ++T+ L L
Sbjct: 294 EDGGLACYGEKEVLEALMMGAVDTLIVSE--ELEKYKVKIACNNCDYLEEKTVNKLELIK 351
Query: 414 EQEKDKSHFTDKESGVELELVECQPLLEWLAK 509
+E+ K+ K G L +VE + +E+L++
Sbjct: 352 LEEELKNAQCPKCGGA-LSIVEEKDYIEYLSE 382
>UniRef50_Q479I8 Cluster: Cation efflux protein; n=1; Dechloromonas
aromatica RCB|Rep: Cation efflux protein - Dechloromonas
aromatica (strain RCB)
Length = 389
Score = 35.1 bits (77), Expect = 2.3
Identities = 17/49 (34%), Positives = 27/49 (55%)
Frame = +3
Query: 363 VLKSHATNQETILHLTPEQEKDKSHFTDKESGVELELVECQPLLEWLAK 509
VL+ H + ++H+ PE + D + F+ + G E L E +PLL L K
Sbjct: 282 VLREHPEVLDVLVHIDPEDDMDPAVFSARLPGREALLAELKPLLAGLPK 330
>UniRef50_Q0QZI1 Cluster: Gp47; n=1; Phage Syn9|Rep: Gp47 - Phage
Syn9
Length = 184
Score = 35.1 bits (77), Expect = 2.3
Identities = 20/52 (38%), Positives = 28/52 (53%)
Frame = +3
Query: 336 WENLDIQRYVLKSHATNQETILHLTPEQEKDKSHFTDKESGVELELVECQPL 491
W N Q VLKSH+ + ++T K + FTD E+G E+ VE +PL
Sbjct: 86 WINFHTQDDVLKSHSHDYPIHGYITLTNHKTDTVFTDGENGKEVWRVENKPL 137
>UniRef50_A2QDN2 Cluster: Contig An02c0230, complete genome; n=1;
Aspergillus niger|Rep: Contig An02c0230, complete genome
- Aspergillus niger
Length = 619
Score = 35.1 bits (77), Expect = 2.3
Identities = 16/40 (40%), Positives = 21/40 (52%), Gaps = 1/40 (2%)
Frame = +1
Query: 484 SRCWSGWPKLQVVWCHLRDH-HGQEPGGKPVRSRLSEGFG 600
SR WS + +W H+ H +GQ P G P + R S G G
Sbjct: 250 SRAWSEQASSRHIWRHVFRHTYGQHPSGLPFKKRQSAGLG 289
>UniRef50_Q5G7G7 Cluster: Polyprotein 1a; n=3; Mint virus 1|Rep:
Polyprotein 1a - Mint virus 1
Length = 2511
Score = 34.7 bits (76), Expect = 3.0
Identities = 29/87 (33%), Positives = 45/87 (51%), Gaps = 3/87 (3%)
Frame = -3
Query: 302 SAQCIIDTEAILSCVLEISSK*R--PISF-FSCINLTFCKDSAASSIA*LKPFSPPYDTS 132
SA+C DT + +S +L+IS K R P SF S I++ +S S K S P+D+
Sbjct: 2400 SARCYDDTASAISTMLDISEKYRTMPRSFETSSISMEVSGESPDDSRC--KALSAPHDSI 2457
Query: 131 TSFMILAWRRGSNISDWDSSVLKSAEP 51
SF+ ++I+ D S S++P
Sbjct: 2458 NSFLNEILEGSNSINFGDLSAEMSSQP 2484
>UniRef50_Q97MW9 Cluster: Uncharacterized conserved protein, YTFE
E.coli ortholog; n=8; Firmicutes|Rep: Uncharacterized
conserved protein, YTFE E.coli ortholog - Clostridium
acetobutylicum
Length = 239
Score = 33.1 bits (72), Expect = 9.2
Identities = 14/33 (42%), Positives = 22/33 (66%)
Frame = -1
Query: 670 LVEFPRSVEAASDWKVQLCNGGDRRILPKAANE 572
+V+FPR++E +K+ C GGD R+L +A E
Sbjct: 15 VVKFPRAIEILKRYKIDFCCGGD-RVLEEAIKE 46
>UniRef50_Q4P4N5 Cluster: Putative uncharacterized protein; n=1;
Ustilago maydis|Rep: Putative uncharacterized protein -
Ustilago maydis (Smut fungus)
Length = 1084
Score = 33.1 bits (72), Expect = 9.2
Identities = 28/92 (30%), Positives = 44/92 (47%)
Frame = +1
Query: 127 LVDVSYGGENGFNQAIELAAESLQNVKFIQEKKLIGRYFDEISRTQESIASVSMIHCALS 306
+ D+ YG N N ++ L AES NV E L R + R Q S + AL
Sbjct: 155 MFDLHYGYANQSN-SLGLKAESGLNVGLNGELDLSQR----VQREQRPFDSATSDALALL 209
Query: 307 NSDPSRR*SAGRTSTYKDTY*SRMPPTRKQFS 402
++ P + S+ +Y+D+ S +PP +K+ S
Sbjct: 210 DNLPFKASSSADNDSYEDSTDSDLPPEKKKLS 241
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 951,702,027
Number of Sequences: 1657284
Number of extensions: 20890575
Number of successful extensions: 59505
Number of sequences better than 10.0: 27
Number of HSP's better than 10.0 without gapping: 56571
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 59490
length of database: 575,637,011
effective HSP length: 100
effective length of database: 409,908,611
effective search space used: 75833093035
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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