Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ps4M0177.Seq
(676 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_P46130 Cluster: Acyl-CoA thioester hydrolase ybgC precu... 219 4e-56
UniRef50_Q47474 Cluster: Pectinesterase B precursor; n=3; Entero... 82 1e-14
UniRef50_Q5MB09 Cluster: Pectin methylesterase; n=1; Sitophilus ... 54 2e-06
UniRef50_P58601 Cluster: Pectinesterase precursor; n=8; Proteoba... 41 0.032
UniRef50_Q4V0F7 Cluster: Pectin methylesterase-like protein; n=2... 39 0.13
UniRef50_Q9Y881 Cluster: Pectin methylesterase; n=4; Eukaryota|R... 36 0.68
UniRef50_Q8X116 Cluster: Pectin methylesterase; n=6; Sclerotinia... 36 0.68
UniRef50_P17872 Cluster: Pectinesterase precursor; n=5; Pezizomy... 36 0.68
UniRef50_A3JDR0 Cluster: Putative uncharacterized protein; n=1; ... 35 1.6
UniRef50_Q8LPF3 Cluster: AT5g47500/MNJ7_9; n=10; Spermatophyta|R... 35 1.6
UniRef50_Q2WGH9 Cluster: Putative uncharacterized protein ycf2; ... 34 2.7
UniRef50_Q4P2M4 Cluster: Putative uncharacterized protein; n=1; ... 34 3.6
UniRef50_Q21X97 Cluster: Putative uncharacterized protein precur... 33 4.8
UniRef50_Q339B8 Cluster: Putative uncharacterized protein; n=3; ... 33 4.8
UniRef50_A6VSI9 Cluster: Filamentous haemagglutinin family outer... 33 6.3
>UniRef50_P46130 Cluster: Acyl-CoA thioester hydrolase ybgC
precursor; n=21; Bacteria|Rep: Acyl-CoA thioester
hydrolase ybgC precursor - Escherichia coli (strain K12)
Length = 427
Score = 219 bits (536), Expect = 4e-56
Identities = 128/227 (56%), Positives = 139/227 (61%), Gaps = 4/227 (1%)
Frame = +1
Query: 4 FASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQXAVDAAIIKRTNKRQYIAVM 183
FASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQ AVDAAIIKRTNKRQYIAVM
Sbjct: 59 FASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVDAAIIKRTNKRQYIAVM 118
Query: 184 PGEYQGTVYVPAAPGGITLYGTGENRLM*RLGFPLMVA*XLXTGVTTSTRAANICQVXXR 363
PGEYQGTVYVPAAPGGITLYGTGE + ++G L
Sbjct: 119 PGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPADWRHDVNPRGKYMPGKPA 178
Query: 364 GICTIXARPNAAXVFGVLCSAVFWSQNXGLXLQNLTIENTLGDR*XQVTSGGGTAY*XVI 543
+ + GVLCSAVFWSQN GL LQNLTIENTLGD V +G A +
Sbjct: 179 WYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENTLGD---SVDAGNHPA--VAL 233
Query: 544 GXD*QCXHSRSSEXLSLXPTIGV----AEPSWXRNRQPATVVTSSYI 672
D + L T V + NRQP T+VT+SYI
Sbjct: 234 RTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVTNSYI 280
Score = 95.1 bits (226), Expect = 1e-18
Identities = 54/119 (45%), Positives = 64/119 (53%), Gaps = 2/119 (1%)
Frame = +3
Query: 255 KPIDVKIGLSLDGGMXPADWXHDVNXRGKYMPGXPXWYMYXXCXTXRSXSIRCSLLCGLL 434
KPIDVKIGLSLDGGM PADW HDVN RGKYMPG P WYMY C + RS SI +LC +
Sbjct: 143 KPIDVKIGLSLDGGMSPADWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSI--GVLCSAV 200
Query: 435 VTKXWPAXAKSD--HRKHAGR*VXAGNIRRWHCVLXGDRXRLTMLXFSVVRXPFFVTNN 605
+ G V AGN GD+ ++ + + FFVTN+
Sbjct: 201 FWSQNNGLQLQNLTIENTLGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNS 259
Score = 45.6 bits (103), Expect = 0.001
Identities = 26/39 (66%), Positives = 26/39 (66%)
Frame = +2
Query: 509 HPAVALRTXW**VQINNVXILGRQXTFLCXQQSVLQNRL 625
HPAVALRT VQINNV ILGRQ TF V QNRL
Sbjct: 228 HPAVALRTDGDQVQINNVNILGRQNTFFVTNSGV-QNRL 265
>UniRef50_Q47474 Cluster: Pectinesterase B precursor; n=3;
Enterobacteriaceae|Rep: Pectinesterase B precursor -
Dickeya dadantii (strain 3937) (Erwinia chrysanthemi
(strain 3937))
Length = 433
Score = 81.8 bits (193), Expect = 1e-14
Identities = 58/164 (35%), Positives = 78/164 (47%), Gaps = 6/164 (3%)
Frame = +1
Query: 19 PGAAAWNPSPI-TLPAQPDFVVGP-AGTQGVTHTTIQXAVDAAIIKRTNK-RQYIAVMPG 189
P A W P+PI T +VVGP AG G THT+IQ AV+AA+ + + R YI ++PG
Sbjct: 68 PVADGWTPTPIDTSRVTAAYVVGPRAGVAGATHTSIQQAVNAALRQHPGQTRVYIKLLPG 127
Query: 190 EYQGTVYVPAAPGGITLYGTGE--NRLM*RLGFPLMVA*XLXTGVTTSTRAANICQVXXR 363
Y GTVYVP +TL+G G+ +++ L M++ Q
Sbjct: 128 TYTGTVYVPEGAPPLTLFGAGDRPEQVVVSLALDSMMS---PADYRARVNPHGQYQPADP 184
Query: 364 GICTIXA-RPNAAXVFGVLCSAVFWSQNXGLXLQNLTIENTLGD 492
A A CSAV WSQ+ L+NLT+ N L D
Sbjct: 185 AWYMYNACATKAGATINTTCSAVMWSQSNDFQLKNLTVVNALLD 228
Score = 52.0 bits (119), Expect = 1e-05
Identities = 22/51 (43%), Positives = 28/51 (54%)
Frame = +3
Query: 255 KPIDVKIGLSLDGGMXPADWXHDVNXRGKYMPGXPXWYMYXXCXTXRSXSI 407
+P V + L+LD M PAD+ VN G+Y P P WYMY C T +I
Sbjct: 150 RPEQVVVSLALDSMMSPADYRARVNPHGQYQPADPAWYMYNACATKAGATI 200
>UniRef50_Q5MB09 Cluster: Pectin methylesterase; n=1; Sitophilus
oryzae|Rep: Pectin methylesterase - Sitophilus oryzae
(Rice weevil)
Length = 382
Score = 54.4 bits (125), Expect = 2e-06
Identities = 42/163 (25%), Positives = 69/163 (42%), Gaps = 2/163 (1%)
Frame = +1
Query: 1 NFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQXAVDAAIIKRTNKRQYIAV 180
N+ + W+P I+ ++ D+ VG + TIQ AV+AAI R+YI +
Sbjct: 35 NYFTTATYLQGWSPPSIST-SKADYTVGNG------YNTIQAAVNAAINAGGTTRKYIKI 87
Query: 181 MPGEYQGTVYVPAAPGGITLYGTGENR--LM*RLGFPLMVA*XLXTGVTTSTRAANICQV 354
G YQ VY+P +T+YG G + + L P T + ++
Sbjct: 88 NAGTYQEVVYIPNTKVPLTIYGGGSSPSDTLITLNMPAQT-----TPSAYKSLVGSLFNS 142
Query: 355 XXRGICTIXARPNAAXVFGVLCSAVFWSQNXGLXLQNLTIENT 483
+ + + G CS VFW + + + NL+IEN+
Sbjct: 143 ADPAYSMYNSWRSKSGAIGTSCSTVFWGKAPAVQIVNLSIENS 185
>UniRef50_P58601 Cluster: Pectinesterase precursor; n=8;
Proteobacteria|Rep: Pectinesterase precursor - Ralstonia
solanacearum (Pseudomonas solanacearum)
Length = 396
Score = 40.7 bits (91), Expect = 0.032
Identities = 24/63 (38%), Positives = 33/63 (52%)
Frame = +1
Query: 70 DFVVGPAGTQGVTHTTIQXAVDAAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGT 249
++ V GT + T+Q A+DAA+ R+YI+V G Y V VP + ITLYG
Sbjct: 79 NYAVAADGT--AQYKTVQAAIDAAVNAGGVARRYISVKAGIYNELVCVPESAPPITLYGL 136
Query: 250 GEN 258
N
Sbjct: 137 DAN 139
>UniRef50_Q4V0F7 Cluster: Pectin methylesterase-like protein; n=2;
Xanthomonas campestris pv. campestris|Rep: Pectin
methylesterase-like protein - Xanthomonas campestris pv.
campestris (strain 8004)
Length = 325
Score = 38.7 bits (86), Expect = 0.13
Identities = 25/70 (35%), Positives = 34/70 (48%)
Frame = +1
Query: 43 SPITLPAQPDFVVGPAGTQGVTHTTIQXAVDAAIIKRTNKRQYIAVMPGEYQGTVYVPAA 222
S A P + V G G + T+Q AVDAA+ + KR I+V G YQ + VPA
Sbjct: 25 STAAFAADPVYTVAKQGNAG--YRTVQAAVDAAV--QGGKRAQISVGAGVYQELLVVPAN 80
Query: 223 PGGITLYGTG 252
+ + G G
Sbjct: 81 APALKMVGAG 90
>UniRef50_Q9Y881 Cluster: Pectin methylesterase; n=4; Eukaryota|Rep:
Pectin methylesterase - Cochliobolus carbonum (Bipolaris
zeicola)
Length = 338
Score = 36.3 bits (80), Expect = 0.68
Identities = 28/85 (32%), Positives = 38/85 (44%), Gaps = 4/85 (4%)
Frame = +1
Query: 4 FASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGV----THTTIQXAVDAAIIKRTNKRQY 171
F SL AA + A D PAG V + T+Q AV+A + K T+K Q
Sbjct: 8 FLSLVAATAAAPAENVLRRASFDRTSAPAGCLTVGGSGKYKTVQSAVNA-LDKSTSKAQC 66
Query: 172 IAVMPGEYQGTVYVPAAPGGITLYG 246
I + G Y VY+ +T+YG
Sbjct: 67 IFIAKGTYTEQVYIQQLNSPLTIYG 91
>UniRef50_Q8X116 Cluster: Pectin methylesterase; n=6;
Sclerotiniaceae|Rep: Pectin methylesterase - Botrytis
cinerea (Noble rot fungus) (Botryotinia fuckeliana)
Length = 348
Score = 36.3 bits (80), Expect = 0.68
Identities = 18/47 (38%), Positives = 25/47 (53%)
Frame = +1
Query: 106 THTTIQXAVDAAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYG 246
++TT Q +DA + T QYI V PG Y VY+P +T+ G
Sbjct: 51 SYTTFQEGIDA-LSTTTTDAQYIFVAPGTYFEQVYLPPLASNLTIQG 96
>UniRef50_P17872 Cluster: Pectinesterase precursor; n=5;
Pezizomycotina|Rep: Pectinesterase precursor -
Aspergillus tubingensis
Length = 331
Score = 36.3 bits (80), Expect = 0.68
Identities = 29/84 (34%), Positives = 40/84 (47%)
Frame = +1
Query: 7 ASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQXAVDAAIIKRTNKRQYIAVMP 186
AS+ A A S +T P+ VV +G + TI AVDA T + Q I +
Sbjct: 7 ASVLFAATALAASRMTAPSGA-IVVAKSGGD---YDTISAAVDALSTTST-ETQTIFIEE 61
Query: 187 GEYQGTVYVPAAPGGITLYGTGEN 258
G Y VY+PA G + +YG E+
Sbjct: 62 GSYDEQVYIPALSGKLIVYGQTED 85
>UniRef50_A3JDR0 Cluster: Putative uncharacterized protein; n=1;
Marinobacter sp. ELB17|Rep: Putative uncharacterized
protein - Marinobacter sp. ELB17
Length = 817
Score = 35.1 bits (77), Expect = 1.6
Identities = 18/51 (35%), Positives = 23/51 (45%)
Frame = -2
Query: 306 QGSCHHQGKAQSSHQSVFTCTVQSNSTRSGRDIYRSLILTRHHGNILALVG 154
+G H G+ + H +N R G D RSL LT H +L LVG
Sbjct: 461 KGEGHEPGQLRMHHAGTGDPLSDANKIRLGTDFLRSLSLTEHFAPLLVLVG 511
>UniRef50_Q8LPF3 Cluster: AT5g47500/MNJ7_9; n=10; Spermatophyta|Rep:
AT5g47500/MNJ7_9 - Arabidopsis thaliana (Mouse-ear
cress)
Length = 362
Score = 35.1 bits (77), Expect = 1.6
Identities = 26/68 (38%), Positives = 35/68 (51%), Gaps = 8/68 (11%)
Frame = +1
Query: 79 VGPAGTQGVT-----HT---TIQXAVDAAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGI 234
VGP+G + +T H ++Q AVD+ I K NK I + PG Y+ V VPA I
Sbjct: 53 VGPSGHKVITVSLNGHAQFRSVQDAVDS-IPKNNNKSITIKIAPGFYREKVVVPATKPYI 111
Query: 235 TLYGTGEN 258
T G G +
Sbjct: 112 TFKGAGRD 119
>UniRef50_Q2WGH9 Cluster: Putative uncharacterized protein ycf2;
n=1; Selaginella uncinata|Rep: Putative uncharacterized
protein ycf2 - Selaginella uncinata (Blue spikemoss)
(Peacock spikemoss)
Length = 1861
Score = 34.3 bits (75), Expect = 2.7
Identities = 19/51 (37%), Positives = 25/51 (49%)
Frame = -3
Query: 359 WXTWHIFAARVDVVTPVXRXHATIKGKPNLHINRFSPVPYRVIPPGAAGTY 207
W + A DV++ R +I G N +N SP P R IPP A+ TY
Sbjct: 256 WFVLQMHPAARDVLSGACRVSVSIFGNQN-RLNANSPTPPRCIPPSASLTY 305
>UniRef50_Q4P2M4 Cluster: Putative uncharacterized protein; n=1;
Ustilago maydis|Rep: Putative uncharacterized protein -
Ustilago maydis (Smut fungus)
Length = 1647
Score = 33.9 bits (74), Expect = 3.6
Identities = 17/47 (36%), Positives = 25/47 (53%)
Frame = +1
Query: 376 IXARPNAAXVFGVLCSAVFWSQNXGLXLQNLTIENTLGDR*XQVTSG 516
+ A P A +FG LC AV + + G+ ++L +EN L D V G
Sbjct: 137 VLAEPEARRIFGQLCLAVAYVHSKGIVHRDLKLENILLDERCNVKLG 183
>UniRef50_Q21X97 Cluster: Putative uncharacterized protein
precursor; n=1; Rhodoferax ferrireducens T118|Rep:
Putative uncharacterized protein precursor - Rhodoferax
ferrireducens (strain DSM 15236 / ATCC BAA-621 / T118)
Length = 79
Score = 33.5 bits (73), Expect = 4.8
Identities = 16/31 (51%), Positives = 20/31 (64%)
Frame = +1
Query: 25 AAAWNPSPITLPAQPDFVVGPAGTQGVTHTT 117
AA P+ +T+PA P V GPAG QG T +T
Sbjct: 17 AACDRPTVVTVPATPVAVPGPAGPQGQTGST 47
>UniRef50_Q339B8 Cluster: Putative uncharacterized protein; n=3;
Oryza sativa (japonica cultivar-group)|Rep: Putative
uncharacterized protein - Oryza sativa subsp. japonica
(Rice)
Length = 453
Score = 33.5 bits (73), Expect = 4.8
Identities = 26/95 (27%), Positives = 40/95 (42%)
Frame = -2
Query: 294 HHQGKAQSSHQSVFTCTVQSNSTRSGRDIYRSLILTRHHGNILALVGTLDNCRIYRXLNR 115
H +G + H + T VQ + G RS R + +LA GT+D C R
Sbjct: 41 HGRGTREVLHAATTTRGVQRRTWTQGGARRRSTEFDRWNQTLLAAAGTVDGCFNREMTRR 100
Query: 114 GMRYTLSARRADNKVRLRRQGNRGRIPRCSTWCQG 10
G + + +D ++ R G+ GR+ R W G
Sbjct: 101 GAQ--VDGGGSDWRIN-RWVGHFGRVARTFQWSIG 132
>UniRef50_A6VSI9 Cluster: Filamentous haemagglutinin family outer
membrane protein; n=1; Marinomonas sp. MWYL1|Rep:
Filamentous haemagglutinin family outer membrane protein
- Marinomonas sp. MWYL1
Length = 2744
Score = 33.1 bits (72), Expect = 6.3
Identities = 17/57 (29%), Positives = 25/57 (43%)
Frame = +1
Query: 70 DFVVGPAGTQGVTHTTIQXAVDAAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITL 240
D+V G G Q TTI A++ T QY + G+ G + A GG+ +
Sbjct: 692 DYVGGLVGQQYTKGTTINAYATGAVVGSTGSGQYAGGLVGDNSGIISKSYATGGVVI 748
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 660,313,609
Number of Sequences: 1657284
Number of extensions: 13144782
Number of successful extensions: 30028
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 28728
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 30022
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 52066120554
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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