Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0061
(392 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_P26267 Cluster: Pyruvate dehydrogenase E1 component sub... 71 1e-11
UniRef50_P08559 Cluster: Pyruvate dehydrogenase E1 component sub... 60 1e-08
UniRef50_Q6NX32 Cluster: Pyruvate dehydrogenase (Lipoamide) alph... 56 3e-07
UniRef50_UPI0000E4A5CB Cluster: PREDICTED: hypothetical protein,... 52 3e-06
UniRef50_Q9W4H4 Cluster: CG7024-PA; n=2; Sophophora|Rep: CG7024-... 46 2e-04
UniRef50_Q4T3C0 Cluster: Chromosome undetermined SCAF10102, whol... 41 0.007
UniRef50_Q23KL2 Cluster: Pyruvate dehydrogenase E1 component; n=... 38 0.091
UniRef50_Q1EGI2 Cluster: Pyruvate dehydrogenase E1 alpha subunit... 37 0.16
UniRef50_A2FVJ6 Cluster: DnaK protein; n=1; Trichomonas vaginali... 34 1.1
UniRef50_UPI0000F2D933 Cluster: PREDICTED: similar to doublecort... 32 4.5
UniRef50_A3LXT8 Cluster: Predicted protein; n=1; Pichia stipitis... 31 6.0
UniRef50_P20193 Cluster: Protein suppressor of variegation 3-7; ... 31 6.0
UniRef50_UPI00005A26AB Cluster: PREDICTED: similar to protease i... 31 7.9
UniRef50_Q9N478 Cluster: Putative uncharacterized protein; n=2; ... 31 7.9
>UniRef50_P26267 Cluster: Pyruvate dehydrogenase E1 component
subunit alpha type I, mitochondrial precursor; n=10;
cellular organisms|Rep: Pyruvate dehydrogenase E1
component subunit alpha type I, mitochondrial precursor
- Ascaris suum (Pig roundworm) (Ascaris lumbricoides)
Length = 396
Score = 70.5 bits (165), Expect = 1e-11
Identities = 33/72 (45%), Positives = 49/72 (68%)
Frame = +2
Query: 176 ITKVTAPVVATNAKYSTKKEATFEIKPYKLHKLDQGPATSATLTSEDALKLYEQLTILRR 355
+ V+ V+A + + ++ EATF+ KP+KLHKLD GP + +T EDA+ Y Q+ +RR
Sbjct: 11 VPTVSPSVMAISVRLAST-EATFQTKPFKLHKLDSGPDINVHVTKEDAVHYYTQMLTIRR 69
Query: 356 IETG*GNLYKEK 391
+E+ GNLYKEK
Sbjct: 70 MESAAGNLYKEK 81
>UniRef50_P08559 Cluster: Pyruvate dehydrogenase E1 component
subunit alpha, somatic form, mitochondrial precursor;
n=110; cellular organisms|Rep: Pyruvate dehydrogenase E1
component subunit alpha, somatic form, mitochondrial
precursor - Homo sapiens (Human)
Length = 390
Score = 60.1 bits (139), Expect = 1e-08
Identities = 31/88 (35%), Positives = 51/88 (57%)
Frame = +2
Query: 128 MSKLIPSAAKFLAGNTITKVTAPVVATNAKYSTKKEATFEIKPYKLHKLDQGPATSATLT 307
M K++ + ++ L+G + + +VA+ + +ATFEIK LH+L++GP + LT
Sbjct: 1 MRKMLAAVSRVLSGASQKPASRVLVASR---NFANDATFEIKKCDLHRLEEGPPVTTVLT 57
Query: 308 SEDALKLYEQLTILRRIETG*GNLYKEK 391
ED LK Y + +RR+E LYK+K
Sbjct: 58 REDGLKYYRMMQTVRRMELKADQLYKQK 85
>UniRef50_Q6NX32 Cluster: Pyruvate dehydrogenase (Lipoamide) alpha
1; n=3; Tetrapoda|Rep: Pyruvate dehydrogenase
(Lipoamide) alpha 1 - Xenopus tropicalis (Western clawed
frog) (Silurana tropicalis)
Length = 369
Score = 55.6 bits (128), Expect = 3e-07
Identities = 28/92 (30%), Positives = 48/92 (52%)
Frame = +2
Query: 116 IIDKMSKLIPSAAKFLAGNTITKVTAPVVATNAKYSTKKEATFEIKPYKLHKLDQGPATS 295
++ +S+++ A+ + +VA+ EATF+IK +H+L++GP T
Sbjct: 4 MLSLLSRVLKGPAQKPVTGAANEAVRVMVASRNYADFASEATFDIKKCDVHRLEEGPPTQ 63
Query: 296 ATLTSEDALKLYEQLTILRRIETG*GNLYKEK 391
A LT E L+ Y + +RR+E LYK+K
Sbjct: 64 AVLTREQGLQYYRTMQTIRRMELKSDQLYKQK 95
>UniRef50_UPI0000E4A5CB Cluster: PREDICTED: hypothetical protein,
partial; n=2; Strongylocentrotus purpuratus|Rep:
PREDICTED: hypothetical protein, partial -
Strongylocentrotus purpuratus
Length = 378
Score = 52.4 bits (120), Expect = 3e-06
Identities = 21/46 (45%), Positives = 32/46 (69%)
Frame = +2
Query: 254 PYKLHKLDQGPATSATLTSEDALKLYEQLTILRRIETG*GNLYKEK 391
P+KLHKL++GP ++ LT ++AL Y ++ +RR+ET LYK K
Sbjct: 165 PFKLHKLEEGPKKTSVLTKDEALDYYHKMQTIRRMETAAATLYKSK 210
>UniRef50_Q9W4H4 Cluster: CG7024-PA; n=2; Sophophora|Rep: CG7024-PA
- Drosophila melanogaster (Fruit fly)
Length = 479
Score = 46.4 bits (105), Expect = 2e-04
Identities = 21/45 (46%), Positives = 28/45 (62%)
Frame = +2
Query: 257 YKLHKLDQGPATSATLTSEDALKLYEQLTILRRIETG*GNLYKEK 391
+K + L+ GP L+ EDAL +Y Q+ LRR ET GN YKE+
Sbjct: 43 FKCYDLENGPTMDVELSREDALTMYTQMLELRRFETVAGNYYKER 87
>UniRef50_Q4T3C0 Cluster: Chromosome undetermined SCAF10102, whole
genome shotgun sequence; n=1; Tetraodon
nigroviridis|Rep: Chromosome undetermined SCAF10102,
whole genome shotgun sequence - Tetraodon nigroviridis
(Green puffer)
Length = 491
Score = 41.1 bits (92), Expect = 0.007
Identities = 18/43 (41%), Positives = 26/43 (60%)
Frame = +2
Query: 263 LHKLDQGPATSATLTSEDALKLYEQLTILRRIETG*GNLYKEK 391
LH+L++GP A LT E L+ Y + +RR+E LYK+K
Sbjct: 5 LHRLEEGPPEKAELTREQGLQYYRTMQTIRRMELKADQLYKQK 47
>UniRef50_Q23KL2 Cluster: Pyruvate dehydrogenase E1 component; n=5;
Intramacronucleata|Rep: Pyruvate dehydrogenase E1
component - Tetrahymena thermophila SB210
Length = 429
Score = 37.5 bits (83), Expect = 0.091
Identities = 23/81 (28%), Positives = 37/81 (45%), Gaps = 1/81 (1%)
Frame = +2
Query: 152 AKFLAGNTITKVTAPVVATNAKYSTKKEATFEIKPYKLHKLDQGPA-TSATLTSEDALKL 328
AK + V KY+ T ++ Y+ LD T +T T E+ LKL
Sbjct: 41 AKLMISKFFNPVAGKTFNRLNKYAFSS-VTINLEGYQTKMLDGFKLPTQSTATKEELLKL 99
Query: 329 YEQLTILRRIETG*GNLYKEK 391
Y+ + ++R+IE LYK++
Sbjct: 100 YKDMNVMRKIELACDKLYKQR 120
>UniRef50_Q1EGI2 Cluster: Pyruvate dehydrogenase E1 alpha subunit;
n=6; Spirotrichea|Rep: Pyruvate dehydrogenase E1 alpha
subunit - Euplotes sp. BB-2004
Length = 389
Score = 36.7 bits (81), Expect = 0.16
Identities = 16/50 (32%), Positives = 29/50 (58%), Gaps = 1/50 (2%)
Frame = +2
Query: 245 EIKPYKLHKLDQGPA-TSATLTSEDALKLYEQLTILRRIETG*GNLYKEK 391
E+ +K+H++++ T AT T + L Y+ + ++RR+E LYK K
Sbjct: 30 ELPKFKVHRIEESELPTKATTTKSELLNYYKDMALMRRVEIVSDMLYKNK 79
>UniRef50_A2FVJ6 Cluster: DnaK protein; n=1; Trichomonas vaginalis
G3|Rep: DnaK protein - Trichomonas vaginalis G3
Length = 726
Score = 33.9 bits (74), Expect = 1.1
Identities = 23/63 (36%), Positives = 34/63 (53%)
Frame = +2
Query: 122 DKMSKLIPSAAKFLAGNTITKVTAPVVATNAKYSTKKEATFEIKPYKLHKLDQGPATSAT 301
D K + +A K LAG +TK+ A + +Y+ K TF KP + +D G A+S T
Sbjct: 161 DSQKKKVSAAIK-LAGLKLTKIIDEKTALSLQYAIDKHDTFVSKPKYVAIIDFG-ASSLT 218
Query: 302 LTS 310
L+S
Sbjct: 219 LSS 221
>UniRef50_UPI0000F2D933 Cluster: PREDICTED: similar to doublecortin
domain containing 5; n=1; Monodelphis domestica|Rep:
PREDICTED: similar to doublecortin domain containing 5 -
Monodelphis domestica
Length = 745
Score = 31.9 bits (69), Expect = 4.5
Identities = 25/92 (27%), Positives = 45/92 (48%), Gaps = 3/92 (3%)
Frame = +2
Query: 80 KDH*IN-LGFIEIIIDKMSKLIP--SAAKFLAGNTITKVTAPVVATNAKYSTKKEATFEI 250
KD IN +G+ + + +K++ + AK L G K P + +K++ F +
Sbjct: 240 KDEEINQVGYYFRLPGRKTKIMVCLACAKSLIGQKEVKKLPPTSKFLCAFGSKEQKQFSL 299
Query: 251 KPYKLHKLDQGPATSATLTSEDALKLYEQLTI 346
K + ++QG AT +EDA++ YE L +
Sbjct: 300 KYLNVIGMNQGDL--ATYEAEDAIRHYEDLLL 329
>UniRef50_A3LXT8 Cluster: Predicted protein; n=1; Pichia
stipitis|Rep: Predicted protein - Pichia stipitis
(Yeast)
Length = 907
Score = 31.5 bits (68), Expect = 6.0
Identities = 22/77 (28%), Positives = 36/77 (46%), Gaps = 6/77 (7%)
Frame = +2
Query: 170 NTITKVTAPVVATNAKYSTKKEATFEIKPYKLHKLDQGPATSATLTSEDALKLY------ 331
N VT P V+ N Y+T+ ++F K YK+ ++ A + + AL L+
Sbjct: 30 NDTFSVTDPYVSINHNYTTQTVSSFNYKTYKVDQVYGSQADQNLIFEKVALPLFNDFLQG 89
Query: 332 EQLTILRRIETG*GNLY 382
+TIL +TG G +
Sbjct: 90 SNVTILAYGQTGTGKTF 106
>UniRef50_P20193 Cluster: Protein suppressor of variegation 3-7;
n=2; Drosophila melanogaster|Rep: Protein suppressor of
variegation 3-7 - Drosophila melanogaster (Fruit fly)
Length = 1250
Score = 31.5 bits (68), Expect = 6.0
Identities = 18/67 (26%), Positives = 30/67 (44%)
Frame = +3
Query: 192 HRL*RQTQNTARKKRRHSKSSPTNSINWIRALQHQPHSLLKMLLSCMNN*RYCGGLKPDE 371
H ++ A K++R S++ N+ NW+R L+ P M + R C E
Sbjct: 270 HEFGTKSYVAAMKQKRISETEAFNNFNWLRWLRWHPWLERSMPTGTIGTCRICSVRMNVE 329
Query: 372 ETYIKKR 392
Y++KR
Sbjct: 330 FVYLRKR 336
>UniRef50_UPI00005A26AB Cluster: PREDICTED: similar to protease
inhibitor 16 precursor isoform 1; n=3; Theria|Rep:
PREDICTED: similar to protease inhibitor 16 precursor
isoform 1 - Canis familiaris
Length = 458
Score = 31.1 bits (67), Expect = 7.9
Identities = 21/69 (30%), Positives = 32/69 (46%)
Frame = +2
Query: 131 SKLIPSAAKFLAGNTITKVTAPVVATNAKYSTKKEATFEIKPYKLHKLDQGPATSATLTS 310
+K +P A + A +++ P A A S E + + L LD+GPATS T
Sbjct: 251 TKALP-AVETKAPSSVATEEPPSTAREAPPSLTTETPSLLVSHSLFSLDEGPATSPKSTH 309
Query: 311 EDALKLYEQ 337
E KL ++
Sbjct: 310 EPISKLADK 318
>UniRef50_Q9N478 Cluster: Putative uncharacterized protein; n=2;
Caenorhabditis|Rep: Putative uncharacterized protein -
Caenorhabditis elegans
Length = 732
Score = 31.1 bits (67), Expect = 7.9
Identities = 18/57 (31%), Positives = 30/57 (52%)
Frame = +2
Query: 122 DKMSKLIPSAAKFLAGNTITKVTAPVVATNAKYSTKKEATFEIKPYKLHKLDQGPAT 292
+K+S + S K L +TKV+A N K + TF + +L +L++GP+T
Sbjct: 579 EKISNITISKTKPLK-KAVTKVSAAKKILNKKLKVNTKKTFGAEDDELEELEEGPST 634
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 363,500,016
Number of Sequences: 1657284
Number of extensions: 6127519
Number of successful extensions: 15405
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 15089
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 15399
length of database: 575,637,011
effective HSP length: 92
effective length of database: 423,166,883
effective search space used: 16080341554
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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