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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30159
         (729 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC1919.09 |tif6||translation initiation factor eIF6|Schizosacc...    87   2e-18
SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme ...    25   8.4  
SPBC17G9.11c |pyr1||pyruvate carboxylase|Schizosaccharomyces pom...    25   8.4  

>SPCC1919.09 |tif6||translation initiation factor
           eIF6|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 244

 Score = 87.0 bits (206), Expect = 2e-18
 Identities = 48/105 (45%), Positives = 68/105 (64%)
 Frame = +2

Query: 236 FLQRVRTELAETIPVIHASVGGCRIIGRMTVGTRTVS*FHLRPRTQNYNTLETVCRRT*N 415
           F      EL + +PV+H ++GG RIIGR+T G R      L P +   N L+ +     +
Sbjct: 34  FYSVFEAELGDVVPVVHTTIGGTRIIGRLTCGNRKGL---LVPSSTTDNELQHLRN---S 87

Query: 416 F*NPQIKTPRGVEERLSALGNVIACNDYVAL*HPDLDKDTEEILA 550
             +P +K  R V+ERLSALGN++ACNDYVAL HPD++++TEEI+A
Sbjct: 88  LPDP-VKIQR-VDERLSALGNIVACNDYVALVHPDIERETEEIIA 130



 Score = 71.7 bits (168), Expect = 1e-13
 Identities = 31/39 (79%), Positives = 36/39 (92%)
 Frame = +3

Query: 138 MAVRVQFENNNEVGVFSKLTNTYCLVAIGGSENFYSVFE 254
           MA+R QFEN+NE+GVFS LTN+Y LVA+GGSENFYSVFE
Sbjct: 1   MALRAQFENSNEIGVFSNLTNSYALVALGGSENFYSVFE 39


>SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1012

 Score = 25.4 bits (53), Expect = 8.4
 Identities = 16/39 (41%), Positives = 23/39 (58%), Gaps = 4/39 (10%)
 Frame = +3

Query: 66  NPREESHHIVKYIIREK-VKRKTKIMAVRVQFE---NNN 170
           NPRE   +I  Y++ EK +  +  IM  R+QF+   NNN
Sbjct: 643 NPREVLENIRDYLVTEKPLSFEECIMWARLQFDKFFNNN 681


>SPBC17G9.11c |pyr1||pyruvate carboxylase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1185

 Score = 25.4 bits (53), Expect = 8.4
 Identities = 9/17 (52%), Positives = 12/17 (70%)
 Frame = -1

Query: 660 YQAPSVGCSTQWGRTKQ 610
           +QA S+G  TQW  TK+
Sbjct: 880 FQATSLGLGTQWAETKK 896


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,195,037
Number of Sequences: 5004
Number of extensions: 68267
Number of successful extensions: 147
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 141
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 145
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 343230174
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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