Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30189.Seq
(763 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 195 5e-51
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 195 7e-51
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 195 7e-51
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 105 7e-24
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 97 2e-21
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 66 7e-12
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 36 0.005
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 36 0.006
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 36 0.008
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 36 0.008
SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 35 0.015
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 32 0.078
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 31 0.18
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 31 0.18
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 30 0.31
SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo... 30 0.41
SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces pomb... 27 2.2
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 195 bits (476), Expect = 5e-51
Identities = 91/141 (64%), Positives = 108/141 (76%)
Frame = +2
Query: 257 IDIALWKFETSKYYVTIIDAPGHXDFIXXMXTGTSQADCAVLIVAAGTGEFEAGISKNGQ 436
IDIALWKFET KY VT+IDAPGH DFI M TGTSQADCAVLI+ GTGEFEAGISK+GQ
Sbjct: 73 IDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAVLIIGGGTGEFEAGISKDGQ 132
Query: 437 TREHALLAFTLGVKQLIVGVNKMDPLNHHTVSPDLRKSXRKYPHTSRRLGYNPAAVAFVP 616
TREHALLA+TLGVKQLIV VNKMD + ++ + +++G+NP V FVP
Sbjct: 133 TREHALLAYTLGVKQLIVAVNKMDTTGWSQAR--FEEIVKETSNFIKKVGFNPKTVPFVP 190
Query: 617 ISGWHGDNMLEPSTKMPWFXG 679
+SG+ GDNM+EP+T MPW+ G
Sbjct: 191 VSGFQGDNMIEPTTNMPWYQG 211
Score = 141 bits (341), Expect = 1e-34
Identities = 64/68 (94%), Positives = 66/68 (97%)
Frame = +1
Query: 43 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 222
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 223 DKLKAERE 246
DKLKAERE
Sbjct: 61 DKLKAERE 68
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 195 bits (475), Expect = 7e-51
Identities = 90/141 (63%), Positives = 108/141 (76%)
Frame = +2
Query: 257 IDIALWKFETSKYYVTIIDAPGHXDFIXXMXTGTSQADCAVLIVAAGTGEFEAGISKNGQ 436
IDIALWKFET KY VT+IDAPGH DFI M TGTSQADCA+LI+ GTGEFEAGISK+GQ
Sbjct: 73 IDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILIIGGGTGEFEAGISKDGQ 132
Query: 437 TREHALLAFTLGVKQLIVGVNKMDPLNHHTVSPDLRKSXRKYPHTSRRLGYNPAAVAFVP 616
TREHALLA+TLGVKQLIV VNKMD + ++ + +++G+NP V FVP
Sbjct: 133 TREHALLAYTLGVKQLIVAVNKMDTTGWSQAR--FEEIVKETSNFIKKVGFNPKTVPFVP 190
Query: 617 ISGWHGDNMLEPSTKMPWFXG 679
+SG+ GDNM+EP+T MPW+ G
Sbjct: 191 VSGFQGDNMIEPTTNMPWYQG 211
Score = 141 bits (341), Expect = 1e-34
Identities = 64/68 (94%), Positives = 66/68 (97%)
Frame = +1
Query: 43 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 222
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 223 DKLKAERE 246
DKLKAERE
Sbjct: 61 DKLKAERE 68
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 195 bits (475), Expect = 7e-51
Identities = 90/141 (63%), Positives = 108/141 (76%)
Frame = +2
Query: 257 IDIALWKFETSKYYVTIIDAPGHXDFIXXMXTGTSQADCAVLIVAAGTGEFEAGISKNGQ 436
IDIALWKFET KY VT+IDAPGH DFI M TGTSQADCA+LI+ GTGEFEAGISK+GQ
Sbjct: 73 IDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILIIGGGTGEFEAGISKDGQ 132
Query: 437 TREHALLAFTLGVKQLIVGVNKMDPLNHHTVSPDLRKSXRKYPHTSRRLGYNPAAVAFVP 616
TREHALLA+TLGVKQLIV VNKMD + ++ + +++G+NP V FVP
Sbjct: 133 TREHALLAYTLGVKQLIVAVNKMDTTGWSQAR--FEEIVKETSNFIKKVGFNPKTVPFVP 190
Query: 617 ISGWHGDNMLEPSTKMPWFXG 679
+SG+ GDNM+EP+T MPW+ G
Sbjct: 191 VSGFQGDNMIEPTTNMPWYQG 211
Score = 141 bits (341), Expect = 1e-34
Identities = 64/68 (94%), Positives = 66/68 (97%)
Frame = +1
Query: 43 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 222
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 223 DKLKAERE 246
DKLKAERE
Sbjct: 61 DKLKAERE 68
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 105 bits (252), Expect = 7e-24
Identities = 55/145 (37%), Positives = 83/145 (57%), Gaps = 4/145 (2%)
Frame = +2
Query: 257 IDIALWKFETSKYYVTIIDAPGHXDFIXXMXTGTSQADCAVLIVAAGTGEFEAGISKNGQ 436
+++ FET +++DAPGH ++ M G SQAD VL+++A GEFEAG + GQ
Sbjct: 304 VEVGRAYFETEHRRFSLLDAPGHKGYVTNMINGASQADIGVLVISARRGEFEAGFERGGQ 363
Query: 437 TREHALLAFTLGVKQLIVGVNKMDPLNHHTVSPDLRKSXRKYPHTSRRL-GYNPAA-VAF 610
TREHA+LA T G+ L+V +NKMD + ++ K RR+ GYN V +
Sbjct: 364 TREHAVLARTQGINHLVVVINKMDEPSVQWSEERYKECVDKLSMFLRRVAGYNSKTDVKY 423
Query: 611 VPISGWHGDNMLE--PSTKMPWFXG 679
+P+S + G N+ + S+ PW+ G
Sbjct: 424 MPVSAYTGQNVKDRVDSSVCPWYQG 448
Score = 80.6 bits (190), Expect = 2e-16
Identities = 35/64 (54%), Positives = 47/64 (73%)
Frame = +1
Query: 55 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 234
K H+NIV IGHVD+GKST G++++ G +DKRT+EK E+EA+E GK S+ +W LD
Sbjct: 236 KEHVNIVFIGHVDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKESWYLSWALDSTS 295
Query: 235 AERE 246
ERE
Sbjct: 296 EERE 299
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 97.5 bits (232), Expect = 2e-21
Identities = 56/145 (38%), Positives = 79/145 (54%), Gaps = 4/145 (2%)
Frame = +2
Query: 257 IDIALWKFETSKYYVTIIDAPGHXDFIXXMXTGTSQADCAVLIVAAGTGEFEAGISKNGQ 436
+D+A FE+ K I DAPGH DFI M G S AD AVL+V + FE G +NGQ
Sbjct: 243 MDVASTTFESDKKIYEIGDAPGHRDFISGMIAGASSADFAVLVVDSSQNNFERGFLENGQ 302
Query: 437 TREHALLAFTLGVKQLIVGVNKMDPLNHHTVSPDLRKSXRKY--PHTSRRLGYNPAAVAF 610
TREHA L LG+ +++V VNK+D ++ S D + + + +G+ + V F
Sbjct: 303 TREHAYLLRALGISEIVVSVNKLDLMSW---SEDRFQEIKNIVSDFLIKMVGFKTSNVHF 359
Query: 611 VPISGWHGDNML--EPSTKMPWFXG 679
VPIS G N++ + S W+ G
Sbjct: 360 VPISAISGTNLIQKDSSDLYKWYKG 384
Score = 72.5 bits (170), Expect = 6e-14
Identities = 31/63 (49%), Positives = 44/63 (69%)
Frame = +1
Query: 55 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 234
K +++VV GHVDSGKST G ++++ G I+ R+++K EA GKGSF YAW+LD +
Sbjct: 175 KPVVHLVVTGHVDSGKSTMLGRIMFELGEINSRSMQKLHNEAANSGKGSFSYAWLLDTTE 234
Query: 235 AER 243
ER
Sbjct: 235 EER 237
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 65.7 bits (153), Expect = 7e-12
Identities = 32/78 (41%), Positives = 49/78 (62%)
Frame = +2
Query: 275 KFETSKYYVTIIDAPGHXDFIXXMXTGTSQADCAVLIVAAGTGEFEAGISKNGQTREHAL 454
++ET+ + +D PGH D+I M TG + D A+++V+A G+ QTREH L
Sbjct: 110 EYETANRHYAHVDCPGHADYIKNMITGAATMDGAIIVVSATDGQMP-------QTREHLL 162
Query: 455 LAFTLGVKQLIVGVNKMD 508
LA +GVKQ++V +NK+D
Sbjct: 163 LARQVGVKQIVVYINKVD 180
Score = 35.1 bits (77), Expect = 0.011
Identities = 16/29 (55%), Positives = 20/29 (68%)
Frame = +1
Query: 49 KEKTHINIVVIGHVDSGKSTTTGHLIYKC 135
++K H+NI IGHVD GK+T T I KC
Sbjct: 49 RKKPHVNIGTIGHVDHGKTTLTA-AITKC 76
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 36.3 bits (80), Expect = 0.005
Identities = 21/57 (36%), Positives = 29/57 (50%)
Frame = +2
Query: 278 FETSKYYVTIIDAPGHXDFIXXMXTGTSQADCAVLIVAAGTGEFEAGISKNGQTREH 448
FE S Y + IID PGH DF + D AVL++ A +G I+ + Q R +
Sbjct: 141 FEKS-YNINIIDTPGHIDFTIEVERALRVLDGAVLVLCAVSGVQSQTITVDRQMRRY 196
Score = 29.1 bits (62), Expect = 0.72
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +1
Query: 67 NIVVIGHVDSGKSTTTGHLIYKCGGI 144
NI + H+DSGK+T T ++Y G I
Sbjct: 61 NIGISAHIDSGKTTFTERVLYYTGRI 86
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 35.9 bits (79), Expect = 0.006
Identities = 20/71 (28%), Positives = 33/71 (46%)
Frame = +2
Query: 296 YVTIIDAPGHXDFIXXMXTGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGV 475
+V+ +D PGH + M G + D A+L++A QT EH + +
Sbjct: 109 HVSFVDCPGHDILMATMLNGAAVMDAALLLIAGNES------CPQPQTSEHLAAIEIMQL 162
Query: 476 KQLIVGVNKMD 508
K +I+ NK+D
Sbjct: 163 KHIIILQNKVD 173
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.008
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +1
Query: 43 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 138
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
Score = 27.9 bits (59), Expect = 1.7
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +2
Query: 293 YYVTIIDAPGHXDFIXXMXTGTSQADCAVLIV 388
+ V +ID+PGH DF + D A+++V
Sbjct: 98 FLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.008
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +1
Query: 43 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 138
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
Score = 27.9 bits (59), Expect = 1.7
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +2
Query: 293 YYVTIIDAPGHXDFIXXMXTGTSQADCAVLIV 388
+ V +ID+PGH DF + D A+++V
Sbjct: 98 FLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129
>SPBC1271.15c |||translation initiation factor
IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual
Length = 686
Score = 34.7 bits (76), Expect = 0.015
Identities = 15/42 (35%), Positives = 21/42 (50%)
Frame = +2
Query: 278 FETSKYYVTIIDAPGHXDFIXXMXTGTSQADCAVLIVAAGTG 403
F+ ++T +D PGH F G + AD VL+VA G
Sbjct: 215 FDKGSKFITFLDTPGHMAFEAMRKRGANIADIVVLVVAGDDG 256
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 32.3 bits (70), Expect = 0.078
Identities = 14/51 (27%), Positives = 26/51 (50%)
Frame = +2
Query: 236 LSVSRYPIDIALWKFETSKYYVTIIDAPGHXDFIXXMXTGTSQADCAVLIV 388
+S+ P+ +A+ + + ID PGH DF+ + + +D VL+V
Sbjct: 190 MSIKSTPLTLAVSDMKGKTFAFQCIDTPGHVDFVDEVAAPMAISDGVVLVV 240
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 31.1 bits (67), Expect = 0.18
Identities = 23/74 (31%), Positives = 37/74 (50%)
Frame = +2
Query: 293 YYVTIIDAPGHXDFIXXMXTGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLG 472
Y + +ID+PGH DF + + + D A ++V A G I+ Q A+
Sbjct: 94 YLINLIDSPGHVDFSSEVSSASRLCDGAFVLVDAVEGVCSQTITVLRQ-------AWIDR 146
Query: 473 VKQLIVGVNKMDPL 514
+K ++V +NKMD L
Sbjct: 147 IKVILV-INKMDRL 159
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 31.1 bits (67), Expect = 0.18
Identities = 26/84 (30%), Positives = 38/84 (45%)
Frame = +2
Query: 257 IDIALWKFETSKYYVTIIDAPGHXDFIXXMXTGTSQADCAVLIVAAGTGEFEAGISKNGQ 436
I+ A F + +ID PGH DF + + D AV I+ + AG+ Q
Sbjct: 81 INSAAISFTWRNQRINLIDTPGHADFTFEVERSVAVLDGAVAII-----DGSAGV--EAQ 133
Query: 437 TREHALLAFTLGVKQLIVGVNKMD 508
T+ A G+ ++I VNKMD
Sbjct: 134 TKVVWKQATKRGIPKVIF-VNKMD 156
Score = 28.7 bits (61), Expect = 0.96
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +1
Query: 67 NIVVIGHVDSGKSTTTGHLIYKCG 138
N+ +I H+D+GK+T T ++Y G
Sbjct: 30 NVGIIAHIDAGKTTLTEKMLYYGG 53
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 30.3 bits (65), Expect = 0.31
Identities = 12/45 (26%), Positives = 22/45 (48%)
Frame = +2
Query: 269 LWKFETSKYYVTIIDAPGHXDFIXXMXTGTSQADCAVLIVAAGTG 403
++ + Y + +ID PGH DF + + + +L+V A G
Sbjct: 116 IYYYHGQSYLLNLIDTPGHVDFRAEVMHSLAACEGCILLVDASQG 160
>SPAC56F8.03 |||translation initiation factor IF2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1079
Score = 29.9 bits (64), Expect = 0.41
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 305 IIDAPGHXDFIXXMXTGTSQADCAVLIV 388
IID PGH F GTS + A+L++
Sbjct: 553 IIDTPGHESFTNLRSRGTSLCNIAILVI 580
>SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 662
Score = 27.5 bits (58), Expect = 2.2
Identities = 19/62 (30%), Positives = 24/62 (38%)
Frame = -1
Query: 286 SFELPESNIDWIPTHAQPLVCPIPKHI*RILYPFPGPPSRTSRWYVCQYHHICRSSDQWW 107
S L N D + V +PK I I++ F P S S C+Y S D W
Sbjct: 12 SSNLRRMNRDHSSNNTNRTVLNLPKEILIIIFSFLDPRSLLSAQCTCKYWKKLLSDDLSW 71
Query: 106 WT 101
T
Sbjct: 72 RT 73
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,110,882
Number of Sequences: 5004
Number of extensions: 63879
Number of successful extensions: 222
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 192
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 213
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 365309308
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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