Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ps4M0211.Seq
(730 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 124 1e-29
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 124 1e-29
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 124 1e-29
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 49 8e-07
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 32 0.096
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 29 0.90
SPAC23A1.04c |mnl1||alpha mannosidase-like protein|Schizosacchar... 28 1.6
SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA syntheta... 27 2.1
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 124 bits (299), Expect = 1e-29
Identities = 73/152 (48%), Positives = 92/152 (60%), Gaps = 1/152 (0%)
Frame = -3
Query: 716 VPGDNVSFNVKKRVRQGIASWLCCW*LPKXTHLRVLTDFTAQVIDX*PSWSNLKRLHTSL 537
+PGDNV FNVK + I C + + FTAQVI + ++ +
Sbjct: 301 LPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCAS-FTAQVIILNHP-GQISAGYSPV 358
Query: 536 GLPHCPH-CLQFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPP 360
H H +FAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ P
Sbjct: 359 LDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAP 418
Query: 359 LGRFAVRDMRQTVAVGVIKAVNFKEAGGGKVT 264
LGRFAVRDMRQTVAVGVIKAV G KVT
Sbjct: 419 LGRFAVRDMRQTVAVGVIKAVEKVAPGAAKVT 450
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 124 bits (299), Expect = 1e-29
Identities = 73/152 (48%), Positives = 92/152 (60%), Gaps = 1/152 (0%)
Frame = -3
Query: 716 VPGDNVSFNVKKRVRQGIASWLCCW*LPKXTHLRVLTDFTAQVIDX*PSWSNLKRLHTSL 537
+PGDNV FNVK + I C + + FTAQVI + ++ +
Sbjct: 301 LPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCAS-FTAQVIILNHP-GQISAGYSPV 358
Query: 536 GLPHCPH-CLQFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPP 360
H H +FAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ P
Sbjct: 359 LDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAP 418
Query: 359 LGRFAVRDMRQTVAVGVIKAVNFKEAGGGKVT 264
LGRFAVRDMRQTVAVGVIKAV G KVT
Sbjct: 419 LGRFAVRDMRQTVAVGVIKAVEKVAPGAAKVT 450
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 124 bits (299), Expect = 1e-29
Identities = 73/152 (48%), Positives = 92/152 (60%), Gaps = 1/152 (0%)
Frame = -3
Query: 716 VPGDNVSFNVKKRVRQGIASWLCCW*LPKXTHLRVLTDFTAQVIDX*PSWSNLKRLHTSL 537
+PGDNV FNVK + I C + + FTAQVI + ++ +
Sbjct: 301 LPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCAS-FTAQVIILNHP-GQISAGYSPV 358
Query: 536 GLPHCPH-CLQFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPP 360
H H +FAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ P
Sbjct: 359 LDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAP 418
Query: 359 LGRFAVRDMRQTVAVGVIKAVNFKEAGGGKVT 264
LGRFAVRDMRQTVAVGVIKAV G KVT
Sbjct: 419 LGRFAVRDMRQTVAVGVIKAVEKVAPGAAKVT 450
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 48.8 bits (111), Expect = 8e-07
Identities = 25/74 (33%), Positives = 43/74 (58%), Gaps = 1/74 (1%)
Frame = -3
Query: 512 LQFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDM 333
+ FA++ K+D+ T + ++ P G I L P+C+E F+++ +GRF +RD
Sbjct: 590 VSFAKLLHKLDK-TNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQ 648
Query: 332 RQTVAVG-VIKAVN 294
TVAVG V+K ++
Sbjct: 649 GTTVAVGKVVKILD 662
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 31.9 bits (69), Expect = 0.096
Identities = 15/31 (48%), Positives = 20/31 (64%)
Frame = -3
Query: 395 PLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 303
PLC+ +E P LGRF +R TVA G++K
Sbjct: 561 PLCLA--EECPALGRFILRRSGDTVAAGIVK 589
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 28.7 bits (61), Expect = 0.90
Identities = 18/53 (33%), Positives = 30/53 (56%)
Frame = +1
Query: 418 MAASPDLMDFGLTSVDLPVRRSTFSLISANCRQCGQCGNPRLVCNRLRFDQDG 576
++ +PDL D L+SVD P++ +T C + GN L+C+ F++DG
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNT----------CSESGNQYLLCD---FNRDG 66
>SPAC23A1.04c |mnl1||alpha mannosidase-like
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 787
Score = 27.9 bits (59), Expect = 1.6
Identities = 12/47 (25%), Positives = 23/47 (48%)
Frame = +3
Query: 408 QVDNGCISRLDGFWVDFSRFTSTTVNFFFDFCKLQAMWAVWQSKTGV 548
+V C + ++FS T+ T N F A ++VW+ ++G+
Sbjct: 198 EVTENCAAAASSLVLEFSMLTALTGNNKFKASAENAFFSVWKRRSGI 244
>SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA synthetases
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 450
Score = 27.5 bits (58), Expect = 2.1
Identities = 11/43 (25%), Positives = 20/43 (46%)
Frame = +1
Query: 430 PDLMDFGLTSVDLPVRRSTFSLISANCRQCGQCGNPRLVCNRL 558
P ++DF + ++ V+ + + CG PR VC+ L
Sbjct: 279 PSMIDFRIGFIEKAVKHPNADSLYVSTIHCGDAEGPRTVCSGL 321
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,929,682
Number of Sequences: 5004
Number of extensions: 57097
Number of successful extensions: 149
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 136
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 140
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 343230174
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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