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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0121.Seq
         (459 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3H5.11 |||NAD/NADH kinase |Schizosaccharomyces pombe|chr 1||...    37   0.001
SPCC24B10.02c |||NAD/NADH kinase|Schizosaccharomyces pombe|chr 3...    36   0.002
SPAC323.01c |||mitochondrial NADH kinase |Schizosaccharomyces po...    36   0.002
SPAC1B1.02c |||NAD/NADH kinase |Schizosaccharomyces pombe|chr 1|...    31   0.085
SPAC25B8.01 |dap1|SPAC26H5.15|cytochrome P450 regulator Dap1|Sch...    25   4.2  
SPBC25B2.06c |btb2||BTB/POZ domain protein Btb2|Schizosaccharomy...    25   4.2  
SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|ch...    25   4.2  
SPAC17G6.17 |pof8||F-box protein Pof8|Schizosaccharomyces pombe|...    25   4.2  
SPBC146.14c |sec26|SPBC337.01c|coatomer beta subunit |Schizosacc...    25   5.6  
SPBC36B7.06c |mug20||sequence orphan|Schizosaccharomyces pombe|c...    25   7.3  
SPCC576.17c |||membrane transporter|Schizosaccharomyces pombe|ch...    24   9.7  
SPBPB7E8.02 |||PSP1 family protein|Schizosaccharomyces pombe|chr...    24   9.7  
SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2 |Schizosacc...    24   9.7  
SPAC9G1.06c |cyk3||cytokinesis protein Cyk3|Schizosaccharomyces ...    24   9.7  
SPAC17H9.13c |||glutamate 5-kinase |Schizosaccharomyces pombe|ch...    24   9.7  

>SPAC3H5.11 |||NAD/NADH kinase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 393

 Score = 37.1 bits (82), Expect = 0.001
 Identities = 20/66 (30%), Positives = 32/66 (48%)
 Frame = +3

Query: 207 GVTGTLXDASSLFPAIVPP*WHSTWARWGFLTPFEFNNFQEQVMNVLEGHAALTLRSRLQ 386
           G  GT+   S LF   VPP         GFLT F+   ++  ++ +      + LR+R +
Sbjct: 148 GGDGTVLYTSRLFQRTVPPIMPFAMGTLGFLTHFDVKKYKTSILEIC-NEMYVHLRTRFE 206

Query: 387 CVVLRK 404
           C V++K
Sbjct: 207 CRVMKK 212



 Score = 28.7 bits (61), Expect = 0.45
 Identities = 23/98 (23%), Positives = 42/98 (42%)
 Frame = +2

Query: 2   DAQILAPFVQLVHWLVHDKSMVVFVEAAVLDDTLLAEYGDFTSVKERLMTFRASTDDLTD 181
           D ++     + V+WL+   ++ VF++ ++ D   L E  +       L+  + S      
Sbjct: 88  DEEVEEKLKEFVYWLISLDNITVFIQKSMED---LFEKTEKIQYWTTLLCTKHS-----Q 139

Query: 182 KIDFIICLGGDRDPXGC*LTFSSNRAAVMAFHLGSLGF 295
             D ++ LGGD         F      +M F +G+LGF
Sbjct: 140 LFDLVLTLGGDGTVLYTSRLFQRTVPPIMPFAMGTLGF 177


>SPCC24B10.02c |||NAD/NADH kinase|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 449

 Score = 36.3 bits (80), Expect = 0.002
 Identities = 25/62 (40%), Positives = 31/62 (50%), Gaps = 1/62 (1%)
 Frame = +3

Query: 207 GVTGTLXDASSLFPAIVPP*WH-STWARWGFLTPFEFNNFQEQVMNVLEGHAALTLRSRL 383
           G   TL   S LF  I PP    S     GFLT F  +N+Q+ +  VL  + +L   SRL
Sbjct: 182 GDNSTLLYTSWLFQKIGPPVLSFSDDDVPGFLTHFSLSNYQQHLYQVLTQNVSLRFCSRL 241

Query: 384 QC 389
           QC
Sbjct: 242 QC 243


>SPAC323.01c |||mitochondrial NADH kinase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 361

 Score = 36.3 bits (80), Expect = 0.002
 Identities = 21/72 (29%), Positives = 36/72 (50%), Gaps = 1/72 (1%)
 Frame = +3

Query: 207 GVTGTLXDASSLFPAI-VPP*WHSTWARWGFLTPFEFNNFQEQVMNVLEGHAALTLRSRL 383
           G  GT+  A+SLF    +PP    +    GFL PF+F +FQ    +     + + +R RL
Sbjct: 132 GGDGTILHAASLFARSGMPPILSFSLGTLGFLLPFDFGSFQTAFADFYNSRSFVLMRMRL 191

Query: 384 QCVVLRKSQDDN 419
           +  +  K  +++
Sbjct: 192 RVAMKTKLYNES 203



 Score = 30.7 bits (66), Expect = 0.11
 Identities = 17/43 (39%), Positives = 24/43 (55%), Gaps = 1/43 (2%)
 Frame = +2

Query: 170 DLTDKIDFIICLGGDRDPXGC*LTFS-SNRAAVMAFHLGSLGF 295
           DL  K+D II +GGD         F+ S    +++F LG+LGF
Sbjct: 120 DLEQKVDAIITVGGDGTILHAASLFARSGMPPILSFSLGTLGF 162


>SPAC1B1.02c |||NAD/NADH kinase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 537

 Score = 31.1 bits (67), Expect = 0.085
 Identities = 17/57 (29%), Positives = 28/57 (49%)
 Frame = +3

Query: 231 ASSLFPAIVPP*WHSTWARWGFLTPFEFNNFQEQVMNVLEGHAALTLRSRLQCVVLR 401
           AS LF  +VPP    + A+ GFL+      + + +  +      + LR R QC ++R
Sbjct: 294 ASWLFQDVVPPVLSFSTAKAGFLSILPIAEYTKTLDLIFHRGFTVNLRMRFQCSIMR 350


>SPAC25B8.01 |dap1|SPAC26H5.15|cytochrome P450 regulator
           Dap1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 166

 Score = 25.4 bits (53), Expect = 4.2
 Identities = 10/20 (50%), Positives = 12/20 (60%)
 Frame = -1

Query: 303 ASENPNEPKWNAITAARLLE 244
           ASE P +P+W   T A L E
Sbjct: 31  ASEEPKQPEWRDYTPAELKE 50


>SPBC25B2.06c |btb2||BTB/POZ domain protein Btb2|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 284

 Score = 25.4 bits (53), Expect = 4.2
 Identities = 16/55 (29%), Positives = 25/55 (45%)
 Frame = -3

Query: 172 IVGASSESHQTFLHRRKVPILRKECVIQNCRLDEHHHAFVMNEPVY*LNEGCQYL 8
           I G +   H  FL R   P+L ++ +  N + D H+   V  E  Y   E C ++
Sbjct: 43  IKGETYHLHALFLGRS--PVLLQKLIENNPKGDVHYTIEVETEDPYVTKESCLFV 95


>SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 538

 Score = 25.4 bits (53), Expect = 4.2
 Identities = 16/52 (30%), Positives = 26/52 (50%), Gaps = 3/52 (5%)
 Frame = +2

Query: 131 VKERLMTFRASTDDLTDKIDFIICLGGDR---DPXGC*LTFSSNRAAVMAFH 277
           V+  ++  +   +++ DKI   + L GDR   D        SSN +AV AF+
Sbjct: 257 VRREMVVEKGLKEEVADKIKEYVLLKGDRSLLDKLEADSLLSSNSSAVAAFN 308


>SPAC17G6.17 |pof8||F-box protein Pof8|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 402

 Score = 25.4 bits (53), Expect = 4.2
 Identities = 13/40 (32%), Positives = 18/40 (45%)
 Frame = +3

Query: 150 LSELAPTISLIRSTSSYAWGVTGTLXDASSLFPAIVPP*W 269
           L E  P +  I +   YA+ +     D S+L    VPP W
Sbjct: 179 LKEFLPYVDAIGTPEGYAFVILYKKVDQSALSKLPVPPGW 218


>SPBC146.14c |sec26|SPBC337.01c|coatomer beta subunit
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 940

 Score = 25.0 bits (52), Expect = 5.6
 Identities = 10/22 (45%), Positives = 13/22 (59%)
 Frame = +2

Query: 167 DDLTDKIDFIICLGGDRDPXGC 232
           +DL  K+D I+ L G  DP  C
Sbjct: 707 EDLASKLDHIVQLAGFTDPVYC 728


>SPBC36B7.06c |mug20||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 169

 Score = 24.6 bits (51), Expect = 7.3
 Identities = 12/39 (30%), Positives = 22/39 (56%)
 Frame = -3

Query: 187 DLISEIVGASSESHQTFLHRRKVPILRKECVIQNCRLDE 71
           +++SE V A SE H + LH   +    K+ ++++ R  E
Sbjct: 18  NMVSEAVLAFSEHHDSALHSYFLNARVKDVILESLRTTE 56


>SPCC576.17c |||membrane transporter|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 525

 Score = 24.2 bits (50), Expect = 9.7
 Identities = 10/23 (43%), Positives = 15/23 (65%)
 Frame = -3

Query: 265 HGGTIAGKSELASXRVPVTPQAY 197
           +G  I  +S+LAS RVP+   A+
Sbjct: 503 YGDRIRARSKLASQRVPINTAAH 525


>SPBPB7E8.02 |||PSP1 family protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 749

 Score = 24.2 bits (50), Expect = 9.7
 Identities = 11/22 (50%), Positives = 11/22 (50%)
 Frame = -2

Query: 296 KTPTSPSGMPSRRHDCWKK*AS 231
           K    PS MPS  H  W K AS
Sbjct: 131 KASLPPSEMPSHFHPLWSKSAS 152


>SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 830

 Score = 24.2 bits (50), Expect = 9.7
 Identities = 13/38 (34%), Positives = 16/38 (42%)
 Frame = -2

Query: 131 PT*SPHTPQGVCHPKLPPRRTPPCFCHERASVLAERRV 18
           P  S ++  G     LPP   PP F  E A  L E  +
Sbjct: 16  PFESENSSLGATPLSLPPSSPPPEFSDEAAEALVEEDI 53


>SPAC9G1.06c |cyk3||cytokinesis protein Cyk3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 886

 Score = 24.2 bits (50), Expect = 9.7
 Identities = 10/22 (45%), Positives = 15/22 (68%)
 Frame = +2

Query: 245 SSNRAAVMAFHLGSLGFSDALR 310
           ++NRAAV+  + G LG S  +R
Sbjct: 735 ANNRAAVLRIYAGRLGVSSPVR 756


>SPAC17H9.13c |||glutamate 5-kinase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 402

 Score = 24.2 bits (50), Expect = 9.7
 Identities = 8/15 (53%), Positives = 11/15 (73%)
 Frame = +3

Query: 81  RQFWMTHSLRSMGTL 125
           R FW+ H L+S G+L
Sbjct: 284 RHFWLLHGLKSHGSL 298


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,802,925
Number of Sequences: 5004
Number of extensions: 33177
Number of successful extensions: 107
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 101
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 107
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 172312850
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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