Silkworm BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbS20012
         (652 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC6G10.08 |idp1||isocitrate dehydrogenase Idp1|Schizosaccharom...   148   8e-37
SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc...    28   1.0  
SPAC11D3.13 |||ThiJ domain protein|Schizosaccharomyces pombe|chr...    27   1.8  
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ...    27   2.3  
SPAC3F10.04 |gsa1|gsh2|glutathione synthetase large subunit Gsa1...    27   2.3  
SPAC21E11.08 |lcb2|SPAC2C4.02|serine palmitoyltransferase |Schiz...    27   2.3  
SPAC6B12.15 |cpc2|rkp1|RACK1 homologue Cpc2|Schizosaccharomyces ...    25   9.5  
SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces ...    25   9.5  
SPAC6F12.02 |rst2||transcription factor Rst2|Schizosaccharomyces...    25   9.5  
SPCC757.12 |||alpha-amylase homolog |Schizosaccharomyces pombe|c...    25   9.5  

>SPAC6G10.08 |idp1||isocitrate dehydrogenase
           Idp1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 418

 Score =  148 bits (358), Expect = 8e-37
 Identities = 69/104 (66%), Positives = 80/104 (76%)
 Frame = +2

Query: 257 DMVAQAIKGSGGFVWACKNYDGDVQSDIVAQGYGSLGMMTSVLMCPDGRTVESESAHGTV 436
           DMVAQAIK +GGFVWACKNYDGDV SD+VAQ YGSLG+MTSVL+ P+GRT ESE+AHGTV
Sbjct: 263 DMVAQAIKSNGGFVWACKNYDGDVMSDVVAQAYGSLGLMTSVLIHPNGRTFESEAAHGTV 322

Query: 437 TRHYRMHQQGKPTSTNPVASIYAWQEVLHTGPN*DGTPELERFA 568
            RHY  + +GK TSTN +ASI+AW   L      DG   L +FA
Sbjct: 323 QRHYMQYLKGKKTSTNSIASIFAWTRGLAHRGRLDGNERLVKFA 366



 Score =  140 bits (339), Expect = 2e-34
 Identities = 61/82 (74%), Positives = 72/82 (87%)
 Frame = +3

Query: 9   LYDFKTPGVAMGMYNTDESIRSFAHSSFQVALQKKWPLYLSTKNTILKRYDGRFKDIFEE 188
           +Y+F   GVAM MYNTD+SIR FAHSSFQ+ALQKK PLYLSTKNTILK+YDGRFKD F+E
Sbjct: 180 VYEFNGSGVAMSMYNTDDSIRGFAHSSFQMALQKKMPLYLSTKNTILKKYDGRFKDTFQE 239

Query: 189 VFQSDYKTKFDEAKIWYEHRLI 254
           V++SDYK KF+E  +WY+HRLI
Sbjct: 240 VYESDYKQKFEELGLWYQHRLI 261



 Score = 32.7 bits (71), Expect = 0.047
 Identities = 16/42 (38%), Positives = 24/42 (57%)
 Frame = +1

Query: 511 RGLAHRAKLGRDSRIGALRS*PSKRRXVECIDSGKMTKDLVI 636
           RGLAHR +L  + R+    +   +   V C++ G MTKDL +
Sbjct: 348 RGLAHRGRLDGNERLVKFAN-ALEHACVRCVEKGIMTKDLYL 388


>SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase
           Cdc25|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 596

 Score = 28.3 bits (60), Expect = 1.0
 Identities = 11/30 (36%), Positives = 20/30 (66%)
 Frame = +3

Query: 132 TKNTILKRYDGRFKDIFEEVFQSDYKTKFD 221
           T+ T+L   DG+FKDIF++    D + +++
Sbjct: 414 TQETLLGLLDGKFKDIFDKCIIIDCRFEYE 443


>SPAC11D3.13 |||ThiJ domain protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 222

 Score = 27.5 bits (58), Expect = 1.8
 Identities = 10/22 (45%), Positives = 16/22 (72%)
 Frame = +2

Query: 107 EKMASIPVYEEYHFETLRWSFQ 172
           ++M  +P +E+YHF+TL   FQ
Sbjct: 156 QQMNLMPTFEKYHFKTLNKLFQ 177


>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 621

 Score = 27.1 bits (57), Expect = 2.3
 Identities = 16/34 (47%), Positives = 19/34 (55%)
 Frame = -1

Query: 103 KAT*KLEWAKDRIDSSVLYMPIATPGVLKSYNTR 2
           KAT K EW K++ID   L   I T   LK+Y  R
Sbjct: 300 KATEKREWVKEKIDLESLLGTIQTN--LKTYQLR 331


>SPAC3F10.04 |gsa1|gsh2|glutathione synthetase large subunit
           Gsa1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 498

 Score = 27.1 bits (57), Expect = 2.3
 Identities = 12/44 (27%), Positives = 22/44 (50%)
 Frame = +3

Query: 102 LQKKWPLYLSTKNTILKRYDGRFKDIFEEVFQSDYKTKFDEAKI 233
           + K W LY   +  +    + +F+ +   VF+SDY    D++ I
Sbjct: 97  MNKLWNLYQKHREAVAHLKENQFQPLSLGVFRSDYMVHQDDSFI 140


>SPAC21E11.08 |lcb2|SPAC2C4.02|serine palmitoyltransferase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 603

 Score = 27.1 bits (57), Expect = 2.3
 Identities = 11/42 (26%), Positives = 21/42 (50%)
 Frame = +2

Query: 311 NYDGDVQSDIVAQGYGSLGMMTSVLMCPDGRTVESESAHGTV 436
           N+ G   + + +QG+ +   + S LMCP    +  E  H ++
Sbjct: 244 NFVGKPAALVFSQGFSTNATVFSTLMCPGSLIISDELNHTSI 285


>SPAC6B12.15 |cpc2|rkp1|RACK1 homologue Cpc2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 314

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 16/51 (31%), Positives = 25/51 (49%)
 Frame = +2

Query: 284 SGGFVWACKNYDGDVQSDIVAQGYGSLGMMTSVLMCPDGRTVESESAHGTV 436
           S G+  A K +D +  S +    YG  G +++V + PDG    S    GT+
Sbjct: 167 SAGWDKAVKVWDLETFS-LRTSHYGHTGYVSAVTISPDGSLCASGGRDGTL 216


>SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 362

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 16/41 (39%), Positives = 21/41 (51%)
 Frame = +2

Query: 311 NYDGDVQSDIVAQGYGSLGMMTSVLMCPDGRTVESESAHGT 433
           N  GD+ SD  A   GSLG++ S  +      V SE  HG+
Sbjct: 252 NLYGDILSDGAASLIGSLGLVPSANV--GDNFVMSEPVHGS 290


>SPAC6F12.02 |rst2||transcription factor Rst2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 567

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 10/35 (28%), Positives = 17/35 (48%)
 Frame = -3

Query: 440 VSPSRAPIRIPRYGHRDTSILMSSFPMTRNPEQQY 336
           +SPS  P  +P Y H ++ +    +P    P + Y
Sbjct: 421 LSPSSIPSGLPTYAHVNSPLGTEQYPSEAYPPKGY 455


>SPCC757.12 |||alpha-amylase homolog |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 625

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 12/32 (37%), Positives = 16/32 (50%)
 Frame = +2

Query: 398 GRTVESESAHGTVTRHYRMHQQGKPTSTNPVA 493
           G T  S S+H   +    +H  G  TST+ VA
Sbjct: 552 GSTSSSGSSHTITSTSQSVHTSGSSTSTSSVA 583


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,916,686
Number of Sequences: 5004
Number of extensions: 62412
Number of successful extensions: 172
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 162
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 171
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 293780908
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)