SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV30087
         (810 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein...    78   1e-15
SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual      65   1e-11
SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr ...    38   0.001
SPBC18H10.08c |ubp4||ubiquitin C-terminal hydrolase Ubp4|Schizos...    27   4.2  
SPBC354.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    26   5.5  
SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce...    26   5.5  
SPCC16C4.18c |taf50||histone H4-like TAF |Schizosaccharomyces po...    26   5.5  
SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32 |Schi...    26   7.3  
SPCC1795.11 |sum3|ded1, slh3, moc2|ATP-dependent RNA helicase Su...    26   7.3  
SPBC12D12.01 |sad1|SPBC16H5.01c|spindle pole body protein Sad1|S...    26   7.3  
SPAC637.09 |||ribonuclease H70 |Schizosaccharomyces pombe|chr 1|...    25   9.6  

>SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein
           Stg1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 174

 Score = 78.2 bits (184), Expect = 1e-15
 Identities = 55/146 (37%), Positives = 77/146 (52%), Gaps = 4/146 (2%)
 Frame = +3

Query: 63  EQEVLTWISDVLGEPLPNGAYE--DVLRDGVILCKLANKLAPGSVKKIQERGTNFQLMEN 236
           E+E   WI + L   L N   +  D L+ GVILC++  K A G+  + +E    F  MEN
Sbjct: 6   EKEAREWIEETLHTKL-NAQLDLLDQLQSGVILCRIC-KEALGANIRYKESNMPFVQMEN 63

Query: 237 IQRF-QAAIKKYGVPEEEIFQTADLFERRNIPQVTLCLYALGRITQKHPEFTGPQLGPKM 413
           I  F   A +   VP +++FQT+DLFERRN  QV   +++  R   K        LGPK+
Sbjct: 64  ISAFINYAQQVVHVPSQDMFQTSDLFERRNDEQVLRSIHSFSRYAAKMFPGKVRGLGPKL 123

Query: 414 ADKNERTFTEEQLRAHNAELN-LQMG 488
           A+K  R F+ +Q R     +N LQ G
Sbjct: 124 AEKKPRVFSAQQQREFREGVNSLQYG 149


>SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1489

 Score = 65.3 bits (152), Expect = 1e-11
 Identities = 35/98 (35%), Positives = 52/98 (53%), Gaps = 2/98 (2%)
 Frame = +3

Query: 69  EVLTWISDVLGEPL-PNGAYEDVLRDGVILCKLANKLAPGSVKKI-QERGTNFQLMENIQ 242
           E   WI + LG  L P   +E  LR+GV+L  L  K  P  + KI       F+  +NI 
Sbjct: 46  EAKKWIEECLGTDLGPTSTFEQSLRNGVVLALLVQKFQPDKLIKIFYSNELQFRHSDNIN 105

Query: 243 RFQAAIKKYGVPEEEIFQTADLFERRNIPQVTLCLYAL 356
           +F   I   G+PE   F+  D++E +N+P+V  C++AL
Sbjct: 106 KFLDFIHGIGLPEIFHFELTDIYEGKNLPKVIYCIHAL 143


>SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 962

 Score = 38.3 bits (85), Expect = 0.001
 Identities = 30/101 (29%), Positives = 45/101 (44%), Gaps = 5/101 (4%)
 Frame = +3

Query: 69  EVLTWISDVLGEPLPN-GAYEDVLRDGVILCKLANKLAPGSVKKIQERGTNFQLMEN--- 236
           E   W+ +       N   + D L +G +LC+LA K  P      + R   +Q+ E    
Sbjct: 69  EAKKWLEEETNNEYQNLDDFVDALVNGKVLCQLAFKYYPKLASNWKPR---YQISERNTV 125

Query: 237 -IQRFQAAIKKYGVPEEEIFQTADLFERRNIPQVTLCLYAL 356
            +  F   +   G+     F+T DL  R NIP+V  CL+AL
Sbjct: 126 YLNAFFHFLDFIGMFTPFRFETKDLVRRFNIPKVIYCLHAL 166


>SPBC18H10.08c |ubp4||ubiquitin C-terminal hydrolase
           Ubp4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 438

 Score = 26.6 bits (56), Expect = 4.2
 Identities = 14/41 (34%), Positives = 22/41 (53%)
 Frame = -3

Query: 232 SMSWKLVPLSWIFFTDPGANLLANLQRITPSLSTSSYAPFG 110
           S+++KL  +      DP    LAN +++  SLS  +Y P G
Sbjct: 33  SLAYKLYSMLRSLKLDPHFMELANPKKVDSSLSYENYQPIG 73


>SPBC354.04 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 163

 Score = 26.2 bits (55), Expect = 5.5
 Identities = 11/28 (39%), Positives = 17/28 (60%)
 Frame = +2

Query: 149 DPLQIGQQISSWISEKNPRERNQLPAHG 232
           D LQ+ ++  SWI+EK   E  +L + G
Sbjct: 11  DSLQVDEKAKSWIAEKVEEELRRLESVG 38


>SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1957

 Score = 26.2 bits (55), Expect = 5.5
 Identities = 10/19 (52%), Positives = 14/19 (73%)
 Frame = -3

Query: 601  FTNTDINVENIGKNLEAIY 545
            F N    VEN+GK++EA+Y
Sbjct: 1855 FDNYSEKVENLGKSIEALY 1873


>SPCC16C4.18c |taf50||histone H4-like TAF |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 452

 Score = 26.2 bits (55), Expect = 5.5
 Identities = 17/64 (26%), Positives = 27/64 (42%)
 Frame = -3

Query: 217 LVPLSWIFFTDPGANLLANLQRITPSLSTSSYAPFGSGSPKTSEIQVRTSCSWSLFLAGI 38
           L+ ++W    +P   +   +Q++ PS+ T   A      P   E         + FL GI
Sbjct: 257 LMHMAWALLDNPNLFVEPYVQQLMPSILTCLVAKRLGSDPNNHEHYALRDL--AAFLLGI 314

Query: 37  VCRR 26
           VC R
Sbjct: 315 VCDR 318


>SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 749

 Score = 25.8 bits (54), Expect = 7.3
 Identities = 11/30 (36%), Positives = 15/30 (50%)
 Frame = -3

Query: 169 LANLQRITPSLSTSSYAPFGSGSPKTSEIQ 80
           L+N   +TPS S S Y P      +T  +Q
Sbjct: 689 LSNFNSVTPSSSASLYPPLIPSEARTPSVQ 718


>SPCC1795.11 |sum3|ded1, slh3, moc2|ATP-dependent RNA helicase
           Sum3|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 636

 Score = 25.8 bits (54), Expect = 7.3
 Identities = 14/52 (26%), Positives = 21/52 (40%)
 Frame = +3

Query: 372 KHPEFTGPQLGPKMADKNERTFTEEQLRAHNAELNLQMGFNKGASQSGHGGF 527
           +   F G   G + + +  R       R      N   G++ G S SG+GGF
Sbjct: 565 RESSFGGNGRGGRYSGRGGRGGNAYGARDFRRPTNSSSGYSSGPSYSGYGGF 616


>SPBC12D12.01 |sad1|SPBC16H5.01c|spindle pole body protein
           Sad1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 514

 Score = 25.8 bits (54), Expect = 7.3
 Identities = 11/32 (34%), Positives = 18/32 (56%)
 Frame = +1

Query: 481 KWASIRAPPSPVMVASVTPATCKLLLNFSQYF 576
           ++  IR+PP+ V + S+TP       NF Q +
Sbjct: 46  RYTKIRSPPTRVSIESITPKQRFPAPNFEQAY 77


>SPAC637.09 |||ribonuclease H70 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 623

 Score = 25.4 bits (53), Expect = 9.6
 Identities = 8/18 (44%), Positives = 11/18 (61%)
 Frame = -3

Query: 343 HNVTWGMLRLSKRSAVWN 290
           HN  WG  R  + +A+WN
Sbjct: 491 HNFVWGRFRELEHAAMWN 508


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,547,817
Number of Sequences: 5004
Number of extensions: 81730
Number of successful extensions: 251
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 238
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 250
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 394431430
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -