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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0098
         (671 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr 1|...   100   2e-22
SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr 3|||Ma...    39   8e-04
SPBP4H10.18c |||sequence orphan|Schizosaccharomyces pombe|chr 2|...    29   0.46 
SPAC17H9.11 |||cofilin/tropomyosin family protein|Schizosaccharo...    29   0.61 
SPCC1450.04 |tef5||translation elongation factor EF-1 beta subun...    27   3.3  
SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi...    26   5.7  
SPAC29A4.10 |rrn5||RNA polymerase I upstream activation factor c...    25   7.5  
SPBP35G2.03c |sgo1||shugoshin Sgo1|Schizosaccharomyces pombe|chr...    25   7.5  
SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr 2||...    25   7.5  
SPAC9.07c |||GTPase Rbg1 |Schizosaccharomyces pombe|chr 1|||Manual     25   9.9  
SPBP26C9.03c |||iron ion transporter |Schizosaccharomyces pombe|...    25   9.9  
SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb...    25   9.9  

>SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 137

 Score =  100 bits (240), Expect = 2e-22
 Identities = 55/140 (39%), Positives = 82/140 (58%)
 Frame = +1

Query: 67  SGVTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTG 246
           SGV VS  C   ++E+K  K  RYVVF + D K   V      + +++ FL DL +    
Sbjct: 4   SGVKVSPECLEAFQELKLGKSLRYVVFKMNDTKTEIVVEKKSTDKDFDTFLGDLPEK--- 60

Query: 247 ECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQ 426
           +CRY ++DFE+ +  +G      + K+  +SW PD A +K KM+YSSS D L+++  G+ 
Sbjct: 61  DCRYAIYDFEF-NLGEGV-----RNKIIFISWSPDVAPIKSKMVYSSSKDTLRRAFTGIG 114

Query: 427 KYIQATDLSEASHEAVEEKL 486
             IQATD SE ++E V EK+
Sbjct: 115 TDIQATDFSEVAYETVLEKV 134


>SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 328

 Score = 38.7 bits (86), Expect = 8e-04
 Identities = 37/137 (27%), Positives = 63/137 (45%), Gaps = 2/137 (1%)
 Frame = +1

Query: 133 RYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTGECRYGLFDFEYTHQCQGTSEAS 312
           R  +  I +E   DV+T+ E++   E    D +K    EC  G  +  +        + S
Sbjct: 26  RAAIISISNENSFDVKTMVEKSESIES---DFKK--VRECLLGSEEPAFVL----VYDDS 76

Query: 313 KKQKLFLMSWCPDTAKVKKKMLYSSSFDALKK--SLVGVQKYIQATDLSEASHEAVEEKL 486
           KK  L L+S+ P+ A V++KMLY+SS  A  +  +L  + +   A+   E  ++ + + L
Sbjct: 77  KKNLLQLISYVPENANVRRKMLYASSRAAFVRCVTLAKLDESYFASTPEELDYQQIMKSL 136

Query: 487 RATDRQ*TAFTHELATK 537
              + Q      EL  K
Sbjct: 137 SKQEDQSPLRQDELERK 153



 Score = 27.9 bits (59), Expect = 1.4
 Identities = 16/56 (28%), Positives = 31/56 (55%), Gaps = 5/56 (8%)
 Frame = +1

Query: 322 KLFLMSWCPDTAKVKKKMLYSSS----FDALKKSL-VGVQKYIQATDLSEASHEAV 474
           K+  +  CP  A VK +M+YSSS     D++K  L + +   I++ D ++ + + +
Sbjct: 244 KILFIYICPMQATVKHRMVYSSSKLGLLDSIKAELGIVIDGKIESNDAADITEKEI 299


>SPBP4H10.18c |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 314

 Score = 29.5 bits (63), Expect = 0.46
 Identities = 40/198 (20%), Positives = 78/198 (39%), Gaps = 3/198 (1%)
 Frame = +1

Query: 40  LRE*HQKMASGVTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGE-RNAEYEQF 216
           LR    ++  G    D+  ++ +E  ++KK +   F  RD++++ ++     R       
Sbjct: 41  LRSEGSELIFGGRSFDSLLSSIKETMEEKKKKSSSFEKRDKRRVQLKEKSPLRTPRNRTQ 100

Query: 217 LEDLQ--KGGTGECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSS 390
           + D +  +  T        +F+   Q   T  A+K     +    P T    K  + ++ 
Sbjct: 101 IIDARSTRKDTENESLRKNNFKRRTQFAFTGRATKSNAGVMDVQSPSTMSTSKNNVRNAE 160

Query: 391 FDALKKSLVGVQKYIQATDLSEASHEAVEEKLRATDRQ*TAFTHELATKPNPLSDTPALT 570
             A KK + G +KY    + S   ++  E K R  DR  + FT         LS   ++ 
Sbjct: 161 RPASKKPVFGSKKYFDVINDSNVENKE-ETKDRNLDRALSKFTQSREKNEQNLSSKASVL 219

Query: 571 TRGHDTTSRLVLLQRENE 624
                   + +LL++ N+
Sbjct: 220 -----RNKKSILLKKRNQ 232


>SPAC17H9.11 |||cofilin/tropomyosin family
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 141

 Score = 29.1 bits (62), Expect = 0.61
 Identities = 17/63 (26%), Positives = 30/63 (47%)
 Frame = +1

Query: 298 TSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLSEASHEAVE 477
           T++      LF++ W P        M+Y+S+     + +  V K  +A D  + + EAV+
Sbjct: 79  TTDGRLSTPLFMIYWRPSATPNDLSMIYASA-KVWFQDVSQVHKVFEARDSEDITSEAVD 137

Query: 478 EKL 486
           E L
Sbjct: 138 EFL 140


>SPCC1450.04 |tef5||translation elongation factor EF-1 beta subunit
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 214

 Score = 26.6 bits (56), Expect = 3.3
 Identities = 10/25 (40%), Positives = 18/25 (72%)
 Frame = +1

Query: 388 SFDALKKSLVGVQKYIQATDLSEAS 462
           S +AL++ L G + Y+Q+TD++  S
Sbjct: 188 SLEALQEELEGFEDYVQSTDIAAMS 212


>SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase
           Lkh1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 690

 Score = 25.8 bits (54), Expect = 5.7
 Identities = 12/44 (27%), Positives = 23/44 (52%), Gaps = 5/44 (11%)
 Frame = -1

Query: 341 HDIRKSFCFLLASDVPWHWCVYSKSNRPY-----LHSPVPPFCR 225
           HD+     +++ S   +H C  ++S  P+     LH+P+P  C+
Sbjct: 107 HDVASHPSYMVQSPTSYHACSNNQSPFPHSHHPPLHNPLPVSCQ 150


>SPAC29A4.10 |rrn5||RNA polymerase I upstream activation factor
           complex subunit Rrn5|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 556

 Score = 25.4 bits (53), Expect = 7.5
 Identities = 22/92 (23%), Positives = 39/92 (42%), Gaps = 4/92 (4%)
 Frame = -1

Query: 557 VSESGFGFVASSCVNAVYWRSVARSFSSTAS*DASERSVAWMYF*TPTR---DFFRASNE 387
           + E  +G ++ SCV+  YW +  +     A     +R +  + +  P++      R  N 
Sbjct: 56  LEEEQWGVLSGSCVDGTYWSAEEKELFFQAVARNGKRDLDLIAYSIPSKSAVQIERYINA 115

Query: 386 LEYNIFFLTLAVSGHHDIR-KSFCFLLASDVP 294
           LE  + +L      H D   +S C L   D+P
Sbjct: 116 LENELRWL----RNHVDASVRSQCLLKYEDIP 143


>SPBP35G2.03c |sgo1||shugoshin Sgo1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 319

 Score = 25.4 bits (53), Expect = 7.5
 Identities = 14/61 (22%), Positives = 31/61 (50%)
 Frame = +1

Query: 94  KTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTGECRYGLFDF 273
           K + + ++ + K ++     +D K+ D + +  +  EY  F ++L+   TG+  Y   DF
Sbjct: 108 KCSSDFLEANVKSQFTTCENKDSKE-DYQILHNKRLEYVSFNDELKSLETGQPLYCFQDF 166

Query: 274 E 276
           +
Sbjct: 167 Q 167


>SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 357

 Score = 25.4 bits (53), Expect = 7.5
 Identities = 13/46 (28%), Positives = 20/46 (43%), Gaps = 2/46 (4%)
 Frame = -1

Query: 293 WHWCVYSKSNRPY--LHSPVPPFCRSSRNCSYSALRSPTVSTSICF 162
           W++  Y+ S R Y      VPP+CR         +R+     + CF
Sbjct: 122 WNFVDYALSLRKYGWTDDVVPPYCRKVLTYKSLGIRTAVYVGAFCF 167


>SPAC9.07c |||GTPase Rbg1 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 366

 Score = 25.0 bits (52), Expect = 9.9
 Identities = 10/22 (45%), Positives = 11/22 (50%)
 Frame = +1

Query: 76  TVSDACKTTYEEIKKDKKHRYV 141
           TV D C   +  IK   KH YV
Sbjct: 316 TVEDFCNNIHSSIKSQFKHAYV 337


>SPBP26C9.03c |||iron ion transporter |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 584

 Score = 25.0 bits (52), Expect = 9.9
 Identities = 14/35 (40%), Positives = 19/35 (54%)
 Frame = +1

Query: 73  VTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDV 177
           VT  D  KT  +  +K  KH+ V  YIR  K +D+
Sbjct: 113 VTNDDGTKTKVDSDEKKHKHKNVRDYIR-FKHVDI 146


>SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1334

 Score = 25.0 bits (52), Expect = 9.9
 Identities = 14/39 (35%), Positives = 17/39 (43%)
 Frame = +3

Query: 483 APRHRSPINSIYTRARDETEPALRHSCPDDTRPRHH*PL 599
           APR R P  ++ T A  E  P  +   P  TR     PL
Sbjct: 24  APRKREPARTVSTPAFMEPAPVSKKPLPPPTRRLPRKPL 62


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,351,825
Number of Sequences: 5004
Number of extensions: 43869
Number of successful extensions: 145
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 139
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 142
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 307866294
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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