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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--0251
         (666 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC8D2.15 |||mitochondrial lipoic acid synthetase |Schizosaccha...   105   4e-24
SPBC1861.02 |abp2||ARS binding protein Abp2|Schizosaccharomyces ...    27   3.2  
SPCC1672.12c |||DUF410 family protein|Schizosaccharomyces pombe|...    27   3.2  
SPBC4F6.07c |||ATP-dependent RNA helicase Mak5 |Schizosaccharomy...    26   5.6  
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo...    25   7.4  
SPBC19F8.07 |crk1|mop1, mcs6|cyclin-dependent kinase activating ...    25   7.4  
SPAC144.02 |||transcription factor |Schizosaccharomyces pombe|ch...    25   9.8  
SPAC56F8.11 |spc3||signal peptidase subunit Spc3 |Schizosaccharo...    25   9.8  
SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyce...    25   9.8  
SPCC584.14 |mug160||conserved eukaryotic protein|Schizosaccharom...    25   9.8  

>SPBC8D2.15 |||mitochondrial lipoic acid synthetase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 370

 Score =  105 bits (253), Expect = 4e-24
 Identities = 55/79 (69%), Positives = 59/79 (74%)
 Frame = +2

Query: 17  ILVECLVPDFRAVRGCVARVASSGLDVFAHNVETVERLTPFVRDPRAGYRQTLKVLQMAK 196
           ILVE L PDF      V  VA SGLDVFAHNVETVE LTPFVRD RA YRQ+L VL+  K
Sbjct: 201 ILVEALTPDFSGRMDLVEIVAKSGLDVFAHNVETVEELTPFVRDRRATYRQSLSVLKHVK 260

Query: 197 EINPDLITKSSIMLGLGET 253
           +  P LITK+SIMLGLGET
Sbjct: 261 KTCPHLITKTSIMLGLGET 279



 Score = 55.6 bits (128), Expect = 6e-09
 Identities = 25/39 (64%), Positives = 30/39 (76%)
 Frame = +1

Query: 256 AQVEQTMKDLREAGVDCVTLGQYMQPTKKHLKVFEYVTP 372
           A++  T+KDL E  VD VT GQYM+PTK+HLKV EYV P
Sbjct: 281 AEILTTLKDLLEHNVDVVTFGQYMRPTKRHLKVQEYVHP 319


>SPBC1861.02 |abp2||ARS binding protein Abp2|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 527

 Score = 26.6 bits (56), Expect = 3.2
 Identities = 12/59 (20%), Positives = 28/59 (47%)
 Frame = -1

Query: 222 FVIKSGFISLAICKTFNVWRYPALGSRTKGVSLSTVSTLCANTSRPLEATRATQPRTAR 46
           + +  G ++  IC  FN+ + P++ S  +   +  V    +N+  PL+   + +   A+
Sbjct: 444 WTVHQGPLTATICHAFNLEKAPSMHSLAEAQMIPDVPISHSNSMEPLDTVSSLKLEIAK 502


>SPCC1672.12c |||DUF410 family protein|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 303

 Score = 26.6 bits (56), Expect = 3.2
 Identities = 17/52 (32%), Positives = 26/52 (50%)
 Frame = +1

Query: 346 LKVFEYVTPAGSAVGGARPQLGFCTWPAEPGALVLQGRRVLHQRRAAGQEGN 501
           L V+E  + A S    AR       +PAE GA     +R+L   ++AG +G+
Sbjct: 67  LDVYEKASYAASLDNKARALTLLGLFPAEEGARKQYVKRLLEWSKSAGPQGD 118


>SPBC4F6.07c |||ATP-dependent RNA helicase Mak5 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 648

 Score = 25.8 bits (54), Expect = 5.6
 Identities = 8/18 (44%), Positives = 15/18 (83%)
 Frame = +2

Query: 95  VFAHNVETVERLTPFVRD 148
           VFA+ +E ++R+TPF+ +
Sbjct: 394 VFANGIEDIKRITPFLNE 411


>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1224

 Score = 25.4 bits (53), Expect = 7.4
 Identities = 10/24 (41%), Positives = 15/24 (62%)
 Frame = -3

Query: 322 TGPALRSPLPPRVDLSWSAPLARG 251
           + PA+ SP PP+V  ++  P A G
Sbjct: 919 SAPAIASPPPPKVGETYHPPTASG 942


>SPBC19F8.07 |crk1|mop1, mcs6|cyclin-dependent kinase activating
           kinase Crk1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 335

 Score = 25.4 bits (53), Expect = 7.4
 Identities = 10/26 (38%), Positives = 16/26 (61%)
 Frame = -1

Query: 366 HVLEHLEVLLRGLHVLAQRYAVHSRL 289
           H+   + +LLRGLH +  R+ +H  L
Sbjct: 109 HIKSWMVMLLRGLHHIHSRFILHRDL 134


>SPAC144.02 |||transcription factor |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 249

 Score = 25.0 bits (52), Expect = 9.8
 Identities = 15/45 (33%), Positives = 19/45 (42%)
 Frame = +3

Query: 222 SRPSCWDSARPRASGADHERSTRGGSGLRNAGPVHAAHEEAPQGV 356
           S P C  S     + A H R+      LR + P+  AH   PQ V
Sbjct: 109 SVPECDRSFTRSDALAKHMRTVHEADTLRPSDPIPKAHPMHPQNV 153


>SPAC56F8.11 |spc3||signal peptidase subunit Spc3
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 185

 Score = 25.0 bits (52), Expect = 9.8
 Identities = 8/25 (32%), Positives = 16/25 (64%)
 Frame = +1

Query: 400 PQLGFCTWPAEPGALVLQGRRVLHQ 474
           P++GF +W A PG+  +   +++ Q
Sbjct: 159 PKMGFLSWGAGPGSYEIPFHKIITQ 183


>SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 844

 Score = 25.0 bits (52), Expect = 9.8
 Identities = 16/65 (24%), Positives = 24/65 (36%)
 Frame = +1

Query: 187 NGQRNKS*FNNEVVHHAGTRRDRAQVEQTMKDLREAGVDCVTLGQYMQPTKKHLKVFEYV 366
           N  R     NN   +H  T+R     E +  D      +C      + P  ++L   EY 
Sbjct: 121 NQSRKNQMSNNSTTYHRETKRRNINAEASTSD------NCNNSNTSVDPMDEYLVTAEYT 174

Query: 367 TPAGS 381
            P+ S
Sbjct: 175 MPSTS 179


>SPCC584.14 |mug160||conserved eukaryotic
           protein|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 431

 Score = 25.0 bits (52), Expect = 9.8
 Identities = 16/57 (28%), Positives = 28/57 (49%)
 Frame = +1

Query: 283 LREAGVDCVTLGQYMQPTKKHLKVFEYVTPAGSAVGGARPQLGFCTWPAEPGALVLQ 453
           +RE  V C  L   +Q   ++ K+   +TP  +    A  + GF ++  + G +VLQ
Sbjct: 369 IREISVLCTRL--LLQNNIENQKIIGGLTPITTTHSDALEEAGFTSYINDKGKVVLQ 423


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,462,197
Number of Sequences: 5004
Number of extensions: 45375
Number of successful extensions: 134
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 131
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 134
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 303841898
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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