SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--0124
         (680 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual    28   1.1  
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha...    27   2.5  
SPCC736.03c |||phenylalanyl-tRNA synthetase|Schizosaccharomyces ...    27   3.3  
SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual        26   4.4  
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc...    26   4.4  
SPBC25H2.03 |||vacuolar protein involved in phosphoinositide met...    26   4.4  
SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual        26   4.4  
SPBC26H8.10 |dis3|rrp44|3'-5' exoribonuclease subunit Dis3 |Schi...    26   4.4  
SPAC20G8.08c |fft1||fun thirty related protein Fft1|Schizosaccha...    26   5.8  
SPAP8A3.13c |||Vid 24 family protein|Schizosaccharomyces pombe|c...    26   5.8  
SPBC6B1.06c |ubp14|ucp2|ubiquitin C-terminal hydrolase Ubp14|Sch...    26   5.8  
SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces pomb...    25   7.7  

>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1236

 Score = 28.3 bits (60), Expect = 1.1
 Identities = 22/75 (29%), Positives = 35/75 (46%)
 Frame = -3

Query: 531 SKKAAASDLPDCISASVSSCIETPKLFPSSIFGIINEIHILSKFESSTSSLMCGLTRCIR 352
           S   ++S +P   S SVSS + +    PSS    I+     S F S+++S    ++  I 
Sbjct: 561 SSGISSSSIPSTFS-SVSSILSSSTSSPSSTSLSISSSSTSSTFSSASTSSPSSISSSIS 619

Query: 351 SLSGLLQGTEPFGST 307
           S S +L    P  S+
Sbjct: 620 SSSTILSSPTPSTSS 634


>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
           Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 884

 Score = 27.1 bits (57), Expect = 2.5
 Identities = 13/51 (25%), Positives = 29/51 (56%), Gaps = 1/51 (1%)
 Frame = +3

Query: 75  LKAEHLIIKGFPEKIVKLNELLETS-NFQNRNLSDVHQDLNIPIPTPPATS 224
           +K E  + +  P++  +  E ++TS +    N+S + +  ++P+PT  AT+
Sbjct: 524 IKEEEAVSENAPQQEERSFEQIKTSIHIAPENISAIEESKSVPLPTSFATT 574


>SPCC736.03c |||phenylalanyl-tRNA synthetase|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 429

 Score = 26.6 bits (56), Expect = 3.3
 Identities = 13/50 (26%), Positives = 26/50 (52%)
 Frame = +3

Query: 138 LETSNFQNRNLSDVHQDLNIPIPTPPATSNNEPNAKRQRWTHRSYPATQP 287
           LE + F +++L D    +   + +   + ++     R RWT+ S+P T+P
Sbjct: 208 LEETKFVSQHLKDTLTTVIHDLVSLAPSISSGAEQVRYRWTYDSFPFTKP 257


>SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 4717

 Score = 26.2 bits (55), Expect = 4.4
 Identities = 12/32 (37%), Positives = 17/32 (53%)
 Frame = +1

Query: 262 IGVIQQLNHRRNTGLCTAEWFSTLQQATQ*SY 357
           I V Q L H  N G C+ +  ST++  +  SY
Sbjct: 416 IPVFQNLEHNINIGRCSLKKLSTIRSCSTNSY 447


>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1233

 Score = 26.2 bits (55), Expect = 4.4
 Identities = 15/32 (46%), Positives = 18/32 (56%), Gaps = 4/32 (12%)
 Frame = +2

Query: 335  NKPLSDL----IHLVKPHIRELVEDSNLLKMW 418
            N+ L DL    IHLVKP++    E  N LK W
Sbjct: 1072 NQELLDLLLFSIHLVKPNVETNDEVCNTLKAW 1103


>SPBC25H2.03 |||vacuolar protein involved in phosphoinositide
           metabolism|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 811

 Score = 26.2 bits (55), Expect = 4.4
 Identities = 14/31 (45%), Positives = 17/31 (54%), Gaps = 2/31 (6%)
 Frame = +1

Query: 259 LIGVIQQL--NHRRNTGLCTAEWFSTLQQAT 345
           LI V+Q+L  N    T LC  EW   LQ+ T
Sbjct: 437 LIEVLQKLLSNDNEETRLCALEWVLLLQRRT 467


>SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1279

 Score = 26.2 bits (55), Expect = 4.4
 Identities = 12/22 (54%), Positives = 14/22 (63%)
 Frame = -1

Query: 533 DQKKQLPLIYLIVSLPVYPLVL 468
           D K Q+P  YL  S PV P+VL
Sbjct: 358 DLKVQIPFAYLDPSAPVNPIVL 379


>SPBC26H8.10 |dis3|rrp44|3'-5' exoribonuclease subunit Dis3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 970

 Score = 26.2 bits (55), Expect = 4.4
 Identities = 10/23 (43%), Positives = 15/23 (65%)
 Frame = +2

Query: 308 VLPNGSVPCNKPLSDLIHLVKPH 376
           VLPNG+      ++D+ H VKP+
Sbjct: 523 VLPNGNYEVGVHIADVTHFVKPN 545


>SPAC20G8.08c |fft1||fun thirty related protein
           Fft1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 944

 Score = 25.8 bits (54), Expect = 5.8
 Identities = 21/91 (23%), Positives = 40/91 (43%), Gaps = 4/91 (4%)
 Frame = +2

Query: 419 ISFMIPKIEDGNNFGVSIQEDTLAEIQSGRSEAAAFFDQISRYFISRAKIVSKVAKYPHI 598
           ++FM+PK+ D N  G+ I              A    ++ISR        + +  K   +
Sbjct: 586 LAFMLPKVFDNNMQGLDIIYKIKTTSDGDIERAYLSQERISRAKTIMNPFILRRRKENVL 645

Query: 599 DDYRRAVRELD----EKEYLSLWLVMCEIRN 679
            D    ++ ++    E+  LSL+L + E++N
Sbjct: 646 SDLPPKIQHVEYCHMEETQLSLYLSVLELKN 676


>SPAP8A3.13c |||Vid 24 family protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 547

 Score = 25.8 bits (54), Expect = 5.8
 Identities = 12/28 (42%), Positives = 17/28 (60%)
 Frame = +3

Query: 156 QNRNLSDVHQDLNIPIPTPPATSNNEPN 239
           Q+ ++S  H  L+IP+P P   SNN  N
Sbjct: 61  QDSSISFTHDPLHIPLPNP---SNNNDN 85


>SPBC6B1.06c |ubp14|ucp2|ubiquitin C-terminal hydrolase
           Ubp14|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 775

 Score = 25.8 bits (54), Expect = 5.8
 Identities = 8/30 (26%), Positives = 20/30 (66%)
 Frame = -3

Query: 285 VELLDNSDESIFDV*HLAHCLTSQVVLVLE 196
           +E+ ++SDE ++D  ++  CL   ++L ++
Sbjct: 91  LEIREDSDEDLYDYFYVPKCLVCNIILDIQ 120


>SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 952

 Score = 25.4 bits (53), Expect = 7.7
 Identities = 18/65 (27%), Positives = 29/65 (44%), Gaps = 2/65 (3%)
 Frame = +3

Query: 45  KVQDYKDSLKLKAEHLI--IKGFPEKIVKLNELLETSNFQNRNLSDVHQDLNIPIPTPPA 218
           ++Q     LKL+ E L   IK   E+   L E LET     +NL +++ +    +     
Sbjct: 319 EIQTQNRDLKLETEKLQDQIKALLERNQSLQEALETVKNDEKNLREMNANYETEMKEARQ 378

Query: 219 TSNNE 233
             NN+
Sbjct: 379 KLNNK 383


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,842,996
Number of Sequences: 5004
Number of extensions: 61515
Number of successful extensions: 205
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 196
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 205
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 313902888
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -