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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060104.seq
         (680 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Sc...   137   2e-33
SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy...    76   4e-15
SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyc...    64   2e-11
SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyc...    63   3e-11
SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch...    56   4e-09
SPCC1795.04c |||20S proteasome component alpha 7|Schizosaccharom...    52   6e-08
SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy...    49   7e-07
SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyc...    30   0.36 
SPAC29A4.15 |||serine-tRNA ligase|Schizosaccharomyces pombe|chr ...    27   2.5  
SPAC806.02c |||Par A family ATPase iron cluster assembly protein...    27   3.3  
SPAC4A8.13c |pts1||20S proteasome component beta 5|Schizosacchar...    26   4.4  
SPBC15C4.02 |||ABC1 kinase family protein|Schizosaccharomyces po...    26   4.4  
SPCC777.11 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||...    26   4.4  

>SPAC6G10.04c |||20S proteasome component alpha 6 subunit
           Pre5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 272

 Score =  137 bits (331), Expect = 2e-33
 Identities = 69/141 (48%), Positives = 88/141 (62%)
 Frame = +1

Query: 91  MFRNQYDSDVTVWSPQGRLHQVGVCNGSCETWFCNHWSEE*GLCGVDSVETRCERLSAYQ 270
           MFRNQYD D T WSPQGRLHQV     + +               + +++   E LS+YQ
Sbjct: 1   MFRNQYDGDATTWSPQGRLHQVEYALEAIKQGSATVGLVSKTHAVLVALKRNAEELSSYQ 60

Query: 271 KKIIPIDEHIGISISGLTADARMLSRYMRTECLNHRYSHDAPMPVGRLISSVGNKMQICT 450
           KK+I ID+HIGI+I+GL  DAR+LS YM+ E L+ +     P+PV RL+S V  K QI T
Sbjct: 61  KKLIRIDDHIGIAIAGLAPDARVLSNYMKQEALSSKTLFTRPIPVRRLMSKVAEKAQINT 120

Query: 451 QRYDKRPLGVGLLVAGYDDQG 513
           Q Y +RP GVG LV GYD+ G
Sbjct: 121 QEYGRRPYGVGFLVIGYDESG 141


>SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 247

 Score = 76.2 bits (179), Expect = 4e-15
 Identities = 49/153 (32%), Positives = 79/153 (51%), Gaps = 8/153 (5%)
 Frame = +1

Query: 91  MFRNQYDSDVTVWSPQGRLHQVGVCNGSCE--TWFCNHWSEE*GLCGVDSVETRCERLSA 264
           M R++YD  V  +SP+GRL QV     + +  +      +++  + GV+   T     S 
Sbjct: 3   MTRSEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGVKTKDAVVLGVEKRLTSPLMESH 62

Query: 265 YQKKIIPIDEHIGISISGLTADARMLSRYMRTECLNHRYSHDAPMPVGRLISSVGN---- 432
             +K+  ID HIG +ISGLTADAR +  + R +  NHR+++D P  +     S+ +    
Sbjct: 63  SVEKLFEIDSHIGCAISGLTADARTIIEHARVQTQNHRFTYDEPQGIESTTQSICDLALR 122

Query: 433 --KMQICTQRYDKRPLGVGLLVAGYDDQGXSYF 525
             + +   +R   RP GV LL+AG D+ G   +
Sbjct: 123 FGEGEDGEERIMSRPFGVALLIAGIDEHGPQLY 155


>SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 244

 Score = 63.7 bits (148), Expect = 2e-11
 Identities = 49/148 (33%), Positives = 72/148 (48%), Gaps = 8/148 (5%)
 Frame = +1

Query: 106 YDSDVTVWSPQGRLHQVGVCNGSCETWFCNHWSEE*GLCG-----VDSVETRCERL--SA 264
           +D  +TV+SP+GRL+QV     +    F N      G+ G     V S +   ++L  ++
Sbjct: 7   FDRTITVFSPEGRLYQVEYAFKA----FNNAGITSVGVTGKNCACVISQKKVPDKLIDAS 62

Query: 265 YQKKIIPIDEHIGISISGLTADARMLSRYMRTECLNHRYSHDAPMPVGRLISSVGNKMQI 444
             K + PI + IG  ++G  ADAR      R+E     Y +  PMP   L   + N  Q+
Sbjct: 63  TVKHVFPITKGIGCVMTGSIADARAQVSRARSEAAEFEYKNGYPMPCDVLAKRMANIAQV 122

Query: 445 CTQRYDKRPLGVGLLVAGYDDQ-GXSYF 525
            TQR   RPLGV + +   DD+ G S F
Sbjct: 123 STQRAAMRPLGVAMTLVAVDDEIGPSLF 150


>SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 259

 Score = 63.3 bits (147), Expect = 3e-11
 Identities = 45/136 (33%), Positives = 67/136 (49%), Gaps = 3/136 (2%)
 Frame = +1

Query: 106 YDSDVTVWSPQGRLHQVGVCNGSCE--TWFCNHWSEE*GLCGVDSVET-RCERLSAYQKK 276
           YD  ++V+SP GRL QV     +    T        E  + GV+     + + +S +QK 
Sbjct: 4   YDRALSVFSPDGRLLQVEYGQEAVRRGTTAIALRGNECIVIGVERKNVPKLQNVSNFQK- 62

Query: 277 IIPIDEHIGISISGLTADARMLSRYMRTECLNHRYSHDAPMPVGRLISSVGNKMQICTQR 456
           I  +D H+ ++ +GL ADAR+L    R E  NH+ +   P+ +  L   V    Q  TQ 
Sbjct: 63  IAMVDNHVCLAFAGLNADARILIDKARVEAQNHKLNLADPVSIEYLTRYVAGVQQKYTQS 122

Query: 457 YDKRPLGVGLLVAGYD 504
              RP GV  L+AG+D
Sbjct: 123 GGVRPFGVSTLIAGFD 138


>SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha
           3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 248

 Score = 56.4 bits (130), Expect = 4e-09
 Identities = 42/141 (29%), Positives = 67/141 (47%), Gaps = 8/141 (5%)
 Frame = +1

Query: 106 YDSDVTVWSPQGRLHQVGVCNGSCETWFCNHWSEE*GLCGVDSVETRCERLSAYQ----- 270
           YDS  T++SP+GRL+QV     +      NH     G+   D +    E+    +     
Sbjct: 5   YDSRTTIFSPEGRLYQVEYALEAI-----NHAGVALGIVAKDGIVLAAEKKVTSKLLEQE 59

Query: 271 ---KKIIPIDEHIGISISGLTADARMLSRYMRTECLNHRYSHDAPMPVGRLISSVGNKMQ 441
              +K+  I +++  +++GLTADA +L  Y R     +  + +  MP  +L+  V +  Q
Sbjct: 60  ESAEKLYHIGDNMLCAVAGLTADANILINYARRVGQQYLQTFNEEMPCEQLVRRVCDLKQ 119

Query: 442 ICTQRYDKRPLGVGLLVAGYD 504
             TQ    RP GV  L AG+D
Sbjct: 120 GYTQYGGLRPFGVSFLYAGWD 140


>SPCC1795.04c |||20S proteasome component alpha
           7|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 253

 Score = 52.4 bits (120), Expect = 6e-08
 Identities = 38/131 (29%), Positives = 60/131 (45%), Gaps = 2/131 (1%)
 Frame = +1

Query: 127 WSPQGRLHQVGVCNGSCETWF-CNHWSEE*G-LCGVDSVETRCERLSAYQKKIIPIDEHI 300
           +SP GRL Q      + E    C     E G +  ++ V T          +I  +D HI
Sbjct: 14  FSPDGRLFQAEYAYKAVENASTCIGIKCEDGVILALEKVVTSKLLKPRVNNRIGSVDRHI 73

Query: 301 GISISGLTADARMLSRYMRTECLNHRYSHDAPMPVGRLISSVGNKMQICTQRYDKRPLGV 480
           GI+ +G   D + + +  R E  + R ++ +P+P   +   +GN +Q+ T     RP GV
Sbjct: 74  GIATTGFIPDGQHIVKRARDEATSWRDNYGSPIPGTVIADRLGNYVQLFTCYSSVRPFGV 133

Query: 481 GLLVAGYDDQG 513
              VA YD +G
Sbjct: 134 MSFVATYDSEG 144


>SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 245

 Score = 48.8 bits (111), Expect = 7e-07
 Identities = 43/164 (26%), Positives = 73/164 (44%), Gaps = 8/164 (4%)
 Frame = +1

Query: 100 NQYDSDVTVWSPQGRLHQVGVCNGSCETWFCNHWSEE*GLCGVDSVETRCER-------L 258
           ++Y   +T +SP G+L Q+     +      N      G+   D V    E+       +
Sbjct: 3   DKYAFSLTTFSPNGKLVQIEYALNAV-----NAGVTSVGIKATDGVVLATEKKPTSELAI 57

Query: 259 SAYQKKIIPIDEHIGISISGLTADARML-SRYMRTECLNHRYSHDAPMPVGRLISSVGNK 435
            A  +K+  I   IG+  SG+  D R+L  +  +     ++  ++   P   L+  + + 
Sbjct: 58  GASLEKVCAITPDIGMVYSGMGPDFRVLVDKSRKVAQTTYKKIYNEYPPTKILVQEIASV 117

Query: 436 MQICTQRYDKRPLGVGLLVAGYDDQGXSYFTRLVRQANYFDCRA 567
           MQ  TQ    RP GV LLVAG D++G S + ++     YF  +A
Sbjct: 118 MQESTQSGGVRPFGVSLLVAGMDEKGPSLY-QVDPSGTYFAWKA 160


>SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 226

 Score = 29.9 bits (64), Expect = 0.36
 Identities = 21/81 (25%), Positives = 41/81 (50%), Gaps = 2/81 (2%)
 Frame = +1

Query: 274 KIIPIDEHIGISISGLTADARMLSRYMRTECLNHR--YSHDAPMPVGRLISSVGNKMQIC 447
           K+  + ++I    SG  AD + ++  ++     +R  + HD  +     ++S     ++C
Sbjct: 57  KLTQLTDNIWCCRSGSAADTQTVADLLKYYLSMYRIQFGHDPSVHTAATLAS-----EMC 111

Query: 448 TQRYDKRPLGVGLLVAGYDDQ 510
            Q  +K  L  GL+VAGYD++
Sbjct: 112 YQ--NKNMLSAGLIVAGYDEK 130


>SPAC29A4.15 |||serine-tRNA ligase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 450

 Score = 27.1 bits (57), Expect = 2.5
 Identities = 16/48 (33%), Positives = 21/48 (43%), Gaps = 3/48 (6%)
 Frame = -1

Query: 260 DNRSQRVSTLSTPHSPYSSDQWLQNQVSQLPLH---TPT*WRRP*GLH 126
           D     ++T   P S Y S +W +    QLPL      T +RR  G H
Sbjct: 230 DEERYLIATSEQPISAYHSGEWFEKPSEQLPLKYAGYSTCYRREAGSH 277


>SPAC806.02c |||Par A family ATPase iron cluster assembly
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 608

 Score = 26.6 bits (56), Expect = 3.3
 Identities = 11/28 (39%), Positives = 16/28 (57%)
 Frame = +1

Query: 280 IPIDEHIGISISGLTADARMLSRYMRTE 363
           +PID   G  I  LT D+ ++  Y +TE
Sbjct: 231 VPIDPKFGEMIENLTPDSNIVHLYSKTE 258


>SPAC4A8.13c |pts1||20S proteasome component beta
           5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 272

 Score = 26.2 bits (55), Expect = 4.4
 Identities = 19/85 (22%), Positives = 38/85 (44%)
 Frame = +1

Query: 271 KKIIPIDEHIGISISGLTADARMLSRYMRTECLNHRYSHDAPMPVGRLISSVGNKMQICT 450
           KK+I I+ ++  +++G  AD +     +  EC  H+  +   + V    S+    +   T
Sbjct: 93  KKVIEINPYLLGTLAGGAADCQFWETVLGMECRLHQLRNKELISV----SAASKILSNIT 148

Query: 451 QRYDKRPLGVGLLVAGYDDQGXSYF 525
             Y    L +G ++AG    G + +
Sbjct: 149 YSYKGYGLSMGTMLAGTGKGGTALY 173


>SPBC15C4.02 |||ABC1 kinase family protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 594

 Score = 26.2 bits (55), Expect = 4.4
 Identities = 12/40 (30%), Positives = 22/40 (55%), Gaps = 1/40 (2%)
 Frame = +3

Query: 276 NYSY**TYWNFYIRSYCRCSYAESLHAHRVSQ-SPIFS*C 392
           NYS    YW+F++RS+   +    +H H +   + ++S C
Sbjct: 14  NYSKRKNYWHFWLRSFMSNNRKFLVHIHLIPHLNSLYSIC 53


>SPCC777.11 |||sequence orphan|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 128

 Score = 26.2 bits (55), Expect = 4.4
 Identities = 9/13 (69%), Positives = 10/13 (76%)
 Frame = -3

Query: 648 QXPWNVFENASSR 610
           Q PWNVF+NA  R
Sbjct: 93  QEPWNVFQNAKKR 105


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,814,754
Number of Sequences: 5004
Number of extensions: 58380
Number of successful extensions: 168
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 153
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 157
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 313902888
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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