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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30100
         (637 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_23734| Best HMM Match : No HMM Matches (HMM E-Value=.)             168   3e-42
SB_23733| Best HMM Match : No HMM Matches (HMM E-Value=.)             167   7e-42
SB_56| Best HMM Match : Actin (HMM E-Value=0)                         167   7e-42
SB_56628| Best HMM Match : Actin (HMM E-Value=0)                      167   9e-42
SB_7187| Best HMM Match : No HMM Matches (HMM E-Value=.)              167   9e-42
SB_7190| Best HMM Match : No HMM Matches (HMM E-Value=.)              165   3e-41
SB_13344| Best HMM Match : Actin (HMM E-Value=1.5e-07)                146   1e-35
SB_19204| Best HMM Match : No HMM Matches (HMM E-Value=.)              73   1e-13
SB_54| Best HMM Match : Actin (HMM E-Value=0)                          60   1e-09
SB_26136| Best HMM Match : Actin (HMM E-Value=7.2e-10)                 54   1e-07
SB_6996| Best HMM Match : Actin (HMM E-Value=1.7e-07)                  51   7e-07
SB_22343| Best HMM Match : No HMM Matches (HMM E-Value=.)              42   3e-04
SB_27656| Best HMM Match : Actin (HMM E-Value=4.79999e-40)             38   0.005
SB_30721| Best HMM Match : No HMM Matches (HMM E-Value=.)              32   0.45 
SB_38754| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   5.5  
SB_20978| Best HMM Match : No HMM Matches (HMM E-Value=.)              27   9.7  

>SB_23734| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 338

 Score =  168 bits (409), Expect = 3e-42
 Identities = 77/82 (93%), Positives = 80/82 (97%)
 Frame = +2

Query: 2   RCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQK 181
           RCPEA+FQPSFLGME+ GIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQK
Sbjct: 219 RCPEAMFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQK 278

Query: 182 EITALAPSTMKIKIIAPPERKY 247
           EI+ALAP TMKIKIIAPPERKY
Sbjct: 279 EISALAPPTMKIKIIAPPERKY 300



 Score = 77.0 bits (181), Expect = 1e-14
 Identities = 34/35 (97%), Positives = 35/35 (100%)
 Frame = +1

Query: 256 IGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 360
           IGGSILASLSTFQQMWISKQEYDESGP+IVHRKCF
Sbjct: 304 IGGSILASLSTFQQMWISKQEYDESGPAIVHRKCF 338


>SB_23733| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 376

 Score =  167 bits (406), Expect = 7e-42
 Identities = 76/82 (92%), Positives = 80/82 (97%)
 Frame = +2

Query: 2   RCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQK 181
           RCPEA+FQPSFLGME+ GIHETTYNSIMKCDVDIRKDLYANTVLSGG+TMYPGIADRMQK
Sbjct: 257 RCPEAMFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYANTVLSGGSTMYPGIADRMQK 316

Query: 182 EITALAPSTMKIKIIAPPERKY 247
           EIT+LAP TMKIKIIAPPERKY
Sbjct: 317 EITSLAPPTMKIKIIAPPERKY 338



 Score = 78.2 bits (184), Expect = 5e-15
 Identities = 35/35 (100%), Positives = 35/35 (100%)
 Frame = +1

Query: 256 IGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 360
           IGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF
Sbjct: 342 IGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 376


>SB_56| Best HMM Match : Actin (HMM E-Value=0)
          Length = 375

 Score =  167 bits (406), Expect = 7e-42
 Identities = 76/82 (92%), Positives = 80/82 (97%)
 Frame = +2

Query: 2   RCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQK 181
           RCPEA+FQPSFLGME+ GIHETTYNSIMKCDVDIRKDLYANTVLSGG+TMYPGIADRMQK
Sbjct: 256 RCPEAMFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYANTVLSGGSTMYPGIADRMQK 315

Query: 182 EITALAPSTMKIKIIAPPERKY 247
           EIT+LAP TMKIKIIAPPERKY
Sbjct: 316 EITSLAPPTMKIKIIAPPERKY 337



 Score = 78.2 bits (184), Expect = 5e-15
 Identities = 35/35 (100%), Positives = 35/35 (100%)
 Frame = +1

Query: 256 IGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 360
           IGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF
Sbjct: 341 IGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 375


>SB_56628| Best HMM Match : Actin (HMM E-Value=0)
          Length = 376

 Score =  167 bits (405), Expect = 9e-42
 Identities = 76/82 (92%), Positives = 80/82 (97%)
 Frame = +2

Query: 2   RCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQK 181
           RCPEA+FQPSFLGME+ GIHETTYNSIMKCDVDIRKDLYANTVLSGG+TMYPGIADRMQK
Sbjct: 257 RCPEAMFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYANTVLSGGSTMYPGIADRMQK 316

Query: 182 EITALAPSTMKIKIIAPPERKY 247
           EI+ALAP TMKIKIIAPPERKY
Sbjct: 317 EISALAPPTMKIKIIAPPERKY 338



 Score = 78.2 bits (184), Expect = 5e-15
 Identities = 35/35 (100%), Positives = 35/35 (100%)
 Frame = +1

Query: 256 IGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 360
           IGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF
Sbjct: 342 IGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 376


>SB_7187| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 349

 Score =  167 bits (405), Expect = 9e-42
 Identities = 76/82 (92%), Positives = 80/82 (97%)
 Frame = +2

Query: 2   RCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQK 181
           RCPEAL QPSFLGME+ GIHETTYNSIMKCDVDIRKDLYANTV+SGGTTMYPG+ADRMQK
Sbjct: 230 RCPEALLQPSFLGMESSGIHETTYNSIMKCDVDIRKDLYANTVMSGGTTMYPGLADRMQK 289

Query: 182 EITALAPSTMKIKIIAPPERKY 247
           EI+ALAPSTMKIKIIAPPERKY
Sbjct: 290 EISALAPSTMKIKIIAPPERKY 311



 Score = 77.0 bits (181), Expect = 1e-14
 Identities = 34/35 (97%), Positives = 35/35 (100%)
 Frame = +1

Query: 256 IGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 360
           IGGSILASLSTFQQMWISKQEYDESGP+IVHRKCF
Sbjct: 315 IGGSILASLSTFQQMWISKQEYDESGPAIVHRKCF 349


>SB_7190| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 375

 Score =  165 bits (401), Expect = 3e-41
 Identities = 75/82 (91%), Positives = 80/82 (97%)
 Frame = +2

Query: 2   RCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQK 181
           RCPEA+FQPSFLGME+ GIHETTYNSIMKCDVDIRKDLYANTVLSGG+TM+PGIADRMQK
Sbjct: 256 RCPEAMFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYANTVLSGGSTMFPGIADRMQK 315

Query: 182 EITALAPSTMKIKIIAPPERKY 247
           EI+ALAP TMKIKIIAPPERKY
Sbjct: 316 EISALAPPTMKIKIIAPPERKY 337



 Score = 78.2 bits (184), Expect = 5e-15
 Identities = 35/35 (100%), Positives = 35/35 (100%)
 Frame = +1

Query: 256 IGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 360
           IGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF
Sbjct: 341 IGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 375


>SB_13344| Best HMM Match : Actin (HMM E-Value=1.5e-07)
          Length = 149

 Score =  146 bits (354), Expect = 1e-35
 Identities = 65/82 (79%), Positives = 74/82 (90%)
 Frame = +2

Query: 2   RCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQK 181
           RCPEA+FQP+FLGMEA GIHE  YN IMKCDVDIRKDLY+N VLSGG+TM+PGIADRMQK
Sbjct: 30  RCPEAMFQPAFLGMEAPGIHEAIYNCIMKCDVDIRKDLYSNCVLSGGSTMFPGIADRMQK 89

Query: 182 EITALAPSTMKIKIIAPPERKY 247
           EI  LA ++MK+K+IAPPERKY
Sbjct: 90  EIAMLANASMKVKVIAPPERKY 111



 Score = 68.5 bits (160), Expect = 4e-12
 Identities = 30/35 (85%), Positives = 32/35 (91%)
 Frame = +1

Query: 256 IGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 360
           IGGSILASLSTFQQMWI+K+EY E GP IVHRKCF
Sbjct: 115 IGGSILASLSTFQQMWIAKEEYHEYGPPIVHRKCF 149


>SB_19204| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 411

 Score = 73.3 bits (172), Expect = 1e-13
 Identities = 30/63 (47%), Positives = 43/63 (68%)
 Frame = +2

Query: 8   PEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEI 187
           PEALFQP  + +E  G+ E  +N+I   D+D R + Y + VLSGG+TMYPG+  R+++EI
Sbjct: 262 PEALFQPHLINVEGVGVAELLFNTIQAADIDTRSEFYKHIVLSGGSTMYPGLPSRLEREI 321

Query: 188 TAL 196
             L
Sbjct: 322 KQL 324


>SB_54| Best HMM Match : Actin (HMM E-Value=0)
          Length = 2486

 Score = 60.1 bits (139), Expect = 1e-09
 Identities = 26/46 (56%), Positives = 33/46 (71%)
 Frame = +2

Query: 2    RCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSG 139
            R  E LFQPS LG +  GIHE+ + SI KCD+D+R +L+ N VLSG
Sbjct: 2301 RAAEPLFQPSLLGRDIDGIHESIFKSIKKCDIDLRAELFHNIVLSG 2346


>SB_26136| Best HMM Match : Actin (HMM E-Value=7.2e-10)
          Length = 543

 Score = 53.6 bits (123), Expect = 1e-07
 Identities = 25/71 (35%), Positives = 38/71 (53%), Gaps = 1/71 (1%)
 Frame = +2

Query: 8   PEALFQPSFLGME-ACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKE 184
           PE  F P F   +    + E   N I  C +D+R+ LY N VLSGG+TM+     R+Q++
Sbjct: 206 PEIFFHPEFSNPDFTTPLSEVVDNVIQNCPIDVRRPLYKNIVLSGGSTMFRDFGRRLQRD 265

Query: 185 ITALAPSTMKI 217
           I     + +K+
Sbjct: 266 IKRTVDARLKM 276



 Score = 37.5 bits (83), Expect = 0.009
 Identities = 24/64 (37%), Positives = 31/64 (48%)
 Frame = +1

Query: 181 GNHSSRPIDNED*DHCSSREEVLRMIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 360
           G    +PI+ +   H   R  V    GGS+LAS   F  +  +K +YDE GPSI     F
Sbjct: 283 GRIKPKPIETQVISHHMQRYAV--WFGGSMLASTPEFYSVCHTKADYDEHGPSICRHNPF 340

Query: 361 *THR 372
             HR
Sbjct: 341 -LHR 343


>SB_6996| Best HMM Match : Actin (HMM E-Value=1.7e-07)
          Length = 240

 Score = 51.2 bits (117), Expect = 7e-07
 Identities = 19/64 (29%), Positives = 39/64 (60%)
 Frame = +2

Query: 38  GMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKI 217
           G  A G+ +    S+   D+DIR  L+ + +++GG T+  G  +R+ +E+ +  P +M++
Sbjct: 146 GSTAMGVTQVVTTSVGMTDIDIRAGLFNSVIVTGGNTLLQGFVERLNRELVSKTPPSMRL 205

Query: 218 KIIA 229
           K+I+
Sbjct: 206 KLIS 209


>SB_22343| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 245

 Score = 42.3 bits (95), Expect = 3e-04
 Identities = 20/44 (45%), Positives = 26/44 (59%)
 Frame = +2

Query: 74  NSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPS 205
           +S++ C +D RK L  N VL GGT M PG   R+ +EI  L  S
Sbjct: 64  DSLLLCPIDTRKTLAENIVLIGGTAMTPGFKHRLMQEIYLLLQS 107


>SB_27656| Best HMM Match : Actin (HMM E-Value=4.79999e-40)
          Length = 367

 Score = 38.3 bits (85), Expect = 0.005
 Identities = 15/28 (53%), Positives = 23/28 (82%)
 Frame = +2

Query: 77  SIMKCDVDIRKDLYANTVLSGGTTMYPG 160
           +I K D+D+R+ LY+N VLSGG+T++ G
Sbjct: 264 AIQKSDLDLRRVLYSNIVLSGGSTLFKG 291


>SB_30721| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 40

 Score = 31.9 bits (69), Expect = 0.45
 Identities = 13/20 (65%), Positives = 15/20 (75%)
 Frame = +2

Query: 8  PEALFQPSFLGMEACGIHET 67
          PE +FQPS LG+E  GI ET
Sbjct: 3  PEIIFQPSMLGLEQAGITET 22


>SB_38754| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 205

 Score = 28.3 bits (60), Expect = 5.5
 Identities = 10/27 (37%), Positives = 17/27 (62%)
 Frame = +2

Query: 122 NTVLSGGTTMYPGIADRMQKEITALAP 202
           N  ++GG TMY     R+++E+ A+ P
Sbjct: 132 NVFVTGGNTMYNNFMARLERELLAIRP 158


>SB_20978| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1683

 Score = 27.5 bits (58), Expect = 9.7
 Identities = 16/50 (32%), Positives = 22/50 (44%), Gaps = 2/50 (4%)
 Frame = +1

Query: 160  NRRPYAKGNHSSRPIDNED*DHCSSRE--EVLRMIGGSILASLSTFQQMW 303
            N RP   G   S P D+     C+  +  EVL +    ++    TFQ MW
Sbjct: 1457 NSRPLVYGGSVSSPTDDLPNIFCAGLDLKEVLALRKSKLVTFRKTFQDMW 1506


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,050,262
Number of Sequences: 59808
Number of extensions: 420218
Number of successful extensions: 909
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 843
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 909
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1596754500
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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