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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0065
         (722 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_1769| Best HMM Match : PK (HMM E-Value=7.9e-19)                    180   1e-45
SB_1468| Best HMM Match : PK (HMM E-Value=2.2e-19)                     74   1e-13
SB_9651| Best HMM Match : No HMM Matches (HMM E-Value=.)               64   1e-10
SB_3182| Best HMM Match : No HMM Matches (HMM E-Value=.)               31   0.95 
SB_42557| Best HMM Match : GAD (HMM E-Value=1.4)                       29   2.9  
SB_26227| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   5.0  
SB_59380| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   6.7  
SB_45575| Best HMM Match : Keratin_B2 (HMM E-Value=8.8)                28   6.7  
SB_8676| Best HMM Match : Peptidase_M18 (HMM E-Value=0)                28   8.8  
SB_4396| Best HMM Match : No HMM Matches (HMM E-Value=.)               28   8.8  
SB_9110| Best HMM Match : DUF321 (HMM E-Value=4.5)                     28   8.8  
SB_4961| Best HMM Match : Suf (HMM E-Value=0.00086)                    28   8.8  

>SB_1769| Best HMM Match : PK (HMM E-Value=7.9e-19)
          Length = 145

 Score =  180 bits (437), Expect = 1e-45
 Identities = 88/131 (67%), Positives = 102/131 (77%)
 Frame = +1

Query: 22  NAGMLGSRKGVNLPGIPVDLPAVSEKDKSDLLFGVEQGVDMIFASFIRNGAALHEIRGIL 201
           N  +LGS+KGVNLP   VDLPAVSE+DK D+ F VE  VDMIFASFIR    + EIR +L
Sbjct: 15  NDALLGSKKGVNLPNCAVDLPAVSEQDKKDIAFAVEMEVDMIFASFIRKPEDIAEIRKLL 74

Query: 202 GEKGKNIKIISKIENHQGMVNLDEIIAESDGIMVARGDLGIEIPPEKVFLAQKTMIARCN 381
            +  KNIKI++KIENH+G+   +EI+  +DGIMVARGDLGIEIP EKVFLAQK MIARCN
Sbjct: 75  EKHAKNIKIVAKIENHEGVRRFNEILEVADGIMVARGDLGIEIPAEKVFLAQKMMIARCN 134

Query: 382 RVGKPVICATQ 414
             G PVICATQ
Sbjct: 135 MKGVPVICATQ 145


>SB_1468| Best HMM Match : PK (HMM E-Value=2.2e-19)
          Length = 211

 Score = 74.1 bits (174), Expect = 1e-13
 Identities = 36/56 (64%), Positives = 41/56 (73%)
 Frame = +1

Query: 22  NAGMLGSRKGVNLPGIPVDLPAVSEKDKSDLLFGVEQGVDMIFASFIRNGAALHEI 189
           N  +LGS+KGVNLP   VDLPAVSE+DK D+ F VE  VDMIFASFIR    + EI
Sbjct: 155 NDALLGSKKGVNLPNCAVDLPAVSEQDKKDIAFAVEMEVDMIFASFIRKPEDIAEI 210


>SB_9651| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 34

 Score = 64.1 bits (149), Expect = 1e-10
 Identities = 30/33 (90%), Positives = 31/33 (93%)
 Frame = +1

Query: 415 MLESMVKKPRPTRAEISDVANAILDGADCVMLS 513
           MLESMV+ PRPTRAE SDVANAILDGADCVMLS
Sbjct: 1   MLESMVQNPRPTRAETSDVANAILDGADCVMLS 33


>SB_3182| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1000

 Score = 31.1 bits (67), Expect = 0.95
 Identities = 16/29 (55%), Positives = 19/29 (65%), Gaps = 1/29 (3%)
 Frame = +1

Query: 64  GIPV-DLPAVSEKDKSDLLFGVEQGVDMI 147
           GIPV  LP   E+DK  LLFG E  VD++
Sbjct: 801 GIPVLTLPGKLERDKKFLLFGEEDAVDVL 829


>SB_42557| Best HMM Match : GAD (HMM E-Value=1.4)
          Length = 366

 Score = 29.5 bits (63), Expect = 2.9
 Identities = 13/32 (40%), Positives = 19/32 (59%)
 Frame = -3

Query: 522 SLPGEHHAVSSVEDGVGYVRDLGSGGTGLLNH 427
           +LP E   V + ++G  Y+ D GSGG G+  H
Sbjct: 303 ALPAEKRKVLADKEGSIYISDAGSGGAGVEFH 334


>SB_26227| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 581

 Score = 28.7 bits (61), Expect = 5.0
 Identities = 19/66 (28%), Positives = 28/66 (42%)
 Frame = -1

Query: 317 RSPRATMIPSDSAIISSKLTIPW*FSILEMILMFLPFSPRMPRISCSAAPLRMNDAKIIS 138
           + P   M+    A +   L + W  S+L  +L FLP S  +PR  C+  P       +I 
Sbjct: 113 KCPLTYMMTVTHARVVIALVVIWIDSVLVSVLPFLPLSSGVPR-ECNYHPTSWWSVMVII 171

Query: 137 TPCSTP 120
           T    P
Sbjct: 172 TNVIIP 177


>SB_59380| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1226

 Score = 28.3 bits (60), Expect = 6.7
 Identities = 21/78 (26%), Positives = 34/78 (43%)
 Frame = -1

Query: 341 FSGGISIPRSPRATMIPSDSAIISSKLTIPW*FSILEMILMFLPFSPRMPRISCSAAPLR 162
           F G I  PR P  T++  D A +  K +I   F +     +  P  PR      +   LR
Sbjct: 43  FKGVIPRPRDPERTLVRLDYAKLQGKSSIDIYFDLGGSSNLLQP--PRFVEEPSAVIALR 100

Query: 161 MNDAKIISTPCSTPKSRS 108
            +  +I  +  +TP +R+
Sbjct: 101 KSTVQINCSTIATPVART 118


>SB_45575| Best HMM Match : Keratin_B2 (HMM E-Value=8.8)
          Length = 271

 Score = 28.3 bits (60), Expect = 6.7
 Identities = 15/37 (40%), Positives = 22/37 (59%)
 Frame = +2

Query: 29  VCSDPGKASTCPAYPWTYPQSPRRTSRTCSSASNKAL 139
           VC++P  + T   YP T   SP RT +  S++ +KAL
Sbjct: 198 VCTNPPFSPTAAPYPVTIVLSPTRT-KNVSASRHKAL 233


>SB_8676| Best HMM Match : Peptidase_M18 (HMM E-Value=0)
          Length = 1161

 Score = 27.9 bits (59), Expect = 8.8
 Identities = 11/20 (55%), Positives = 12/20 (60%)
 Frame = -3

Query: 438 LLNHGFQHLSRTDHWFSNPI 379
           LL  GFQ LS  DHW   P+
Sbjct: 859 LLTAGFQELSERDHWDVKPL 878


>SB_4396| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 556

 Score = 27.9 bits (59), Expect = 8.8
 Identities = 10/30 (33%), Positives = 17/30 (56%)
 Frame = +3

Query: 222 QDHLQDRKSPGNGQFRRDYS*IRWYHGCSR 311
           ++HL + K+P + Q +   S   W  GC+R
Sbjct: 128 ENHLNEHKTPSSPQEQSSLSDANWQDGCTR 157


>SB_9110| Best HMM Match : DUF321 (HMM E-Value=4.5)
          Length = 199

 Score = 27.9 bits (59), Expect = 8.8
 Identities = 17/51 (33%), Positives = 28/51 (54%), Gaps = 1/51 (1%)
 Frame = +3

Query: 327 DPSRKGIPRPEDHDRQMQSGWKTSDLCDSNA-GIHG*EAPSHQSRDL*RSQ 476
           DP  KG+P+P+  D +++S      L DS   G+   + P+ + +DL R Q
Sbjct: 15  DPEVKGLPKPQLPDPEVES-LPQPQLSDSEVKGLPQAQLPNPEMKDLSRPQ 64


>SB_4961| Best HMM Match : Suf (HMM E-Value=0.00086)
          Length = 809

 Score = 27.9 bits (59), Expect = 8.8
 Identities = 29/117 (24%), Positives = 51/117 (43%), Gaps = 7/117 (5%)
 Frame = -3

Query: 567 VGHGVNTLD-RIVALGSLPGEHHAVSSVEDGVGYVRDLGSGGTGLLNHGFQHLS------ 409
           V H + +LD R+V + +    HH++ S++D     R +G   T  ++H  + L       
Sbjct: 464 VHHSLKSLDDRLVGVENTVWVHHSLKSLDD-----RLVGVENTVWVHHSLKSLDDRLVGV 518

Query: 408 RTDHWFSNPIASGDHGLLGEEYLFWRDLDTQISASNHDTIGFSYNLV*IDHSLVIFD 238
               W  + + S D  L+G E   W  +   + + +   +G   N V + HSL   D
Sbjct: 519 ENTVWVHHSLKSLDDRLVGVENTVW--VHHSLKSLDDRLVGVE-NTVWVHHSLKSLD 572



 Score = 27.9 bits (59), Expect = 8.8
 Identities = 29/117 (24%), Positives = 51/117 (43%), Gaps = 7/117 (5%)
 Frame = -3

Query: 567 VGHGVNTLD-RIVALGSLPGEHHAVSSVEDGVGYVRDLGSGGTGLLNHGFQHLS------ 409
           V H + +LD R+V + +    HH++ S++D     R +G   T  ++H  + L       
Sbjct: 544 VHHSLKSLDDRLVGVENTVWVHHSLKSLDD-----RLVGVENTVWVHHSLKSLDDRLVGV 598

Query: 408 RTDHWFSNPIASGDHGLLGEEYLFWRDLDTQISASNHDTIGFSYNLV*IDHSLVIFD 238
               W  + + S D  L+G E   W  +   + + +   +G   N V + HSL   D
Sbjct: 599 ENTVWVHHSLKSLDDRLVGVENTVW--VHHSLKSLDDRLVGVE-NTVWVHHSLKSLD 652


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 21,836,329
Number of Sequences: 59808
Number of extensions: 461942
Number of successful extensions: 1571
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1476
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1562
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1925890720
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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