Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--0229
(666 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_8302| Best HMM Match : G-alpha (HMM E-Value=0) 130 8e-31
SB_977| Best HMM Match : No HMM Matches (HMM E-Value=.) 129 2e-30
SB_49453| Best HMM Match : No HMM Matches (HMM E-Value=.) 118 3e-27
SB_30236| Best HMM Match : No HMM Matches (HMM E-Value=.) 104 6e-23
SB_19713| Best HMM Match : No HMM Matches (HMM E-Value=.) 91 7e-19
SB_14105| Best HMM Match : G-alpha (HMM E-Value=0) 44 1e-04
SB_56255| Best HMM Match : Arf (HMM E-Value=0) 42 5e-04
SB_42281| Best HMM Match : No HMM Matches (HMM E-Value=.) 41 8e-04
SB_57342| Best HMM Match : Arf (HMM E-Value=0) 39 0.004
SB_56254| Best HMM Match : Arf (HMM E-Value=5.3e-31) 39 0.004
SB_4844| Best HMM Match : G-alpha (HMM E-Value=3.6e-10) 39 0.004
SB_53421| Best HMM Match : Arf (HMM E-Value=0) 36 0.030
SB_11310| Best HMM Match : Arf (HMM E-Value=0) 35 0.052
SB_37042| Best HMM Match : Arf (HMM E-Value=0) 34 0.12
SB_214| Best HMM Match : Arf (HMM E-Value=0) 34 0.12
SB_7588| Best HMM Match : DcpS (HMM E-Value=0) 33 0.21
SB_18358| Best HMM Match : Arf (HMM E-Value=0) 32 0.37
SB_4906| Best HMM Match : Trypsin (HMM E-Value=0) 31 1.1
SB_16163| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 1.9
SB_5257| Best HMM Match : SlyX (HMM E-Value=2.2) 29 4.5
SB_2235| Best HMM Match : Arf (HMM E-Value=3.1e-13) 29 4.5
SB_47990| Best HMM Match : RRM_1 (HMM E-Value=7.8e-28) 28 7.9
>SB_8302| Best HMM Match : G-alpha (HMM E-Value=0)
Length = 315
Score = 130 bits (315), Expect = 8e-31
Identities = 69/122 (56%), Positives = 85/122 (69%), Gaps = 5/122 (4%)
Frame = +3
Query: 189 KALGRLWR-SGVLWSFQRVPTQR---SAKYFLDDLDRLGAKDYQPTEQDILRTXVKTTGI 356
+++ RLW+ +GV F R + SAKY+LD L R+GA +Y+ TEQD+LRT VKTTGI
Sbjct: 126 ESMERLWKDAGVQQCFHRSREYQLGDSAKYYLDSLQRIGAPNYEATEQDLLRTRVKTTGI 185
Query: 357 XEVHFSFKNLNFKLFDVGGQXSERKKWIHCFEDVTAIIFXVPCLNMXRXC-TG*NTNRMQ 533
EV F +K L+FKLFDVGGQ SERKKWIHCFEDVTAII+ V +TNRM+
Sbjct: 186 VEVQFDYKRLHFKLFDVGGQRSERKKWIHCFEDVTAIIYCVALSAYDLSLEEDESTNRME 245
Query: 534 ES 539
ES
Sbjct: 246 ES 247
Score = 99.5 bits (237), Expect = 2e-21
Identities = 47/79 (59%), Positives = 58/79 (73%)
Frame = +1
Query: 19 VYSNTIQSLVAILRAMPNLGIIYGNRDRESDGKMVFDVIQRMEDTEPFSEELLAAMKRLW 198
V+SNTI SL+ I+RAM L I + N R D K+VFDVI R +DTEPF+ EL +M+RLW
Sbjct: 73 VFSNTIYSLLTIIRAMDKLRIDFENDARLMDAKLVFDVIARADDTEPFTPELTESMERLW 132
Query: 199 ADSGVQECFGRSNEYQLND 255
D+GVQ+CF RS EYQL D
Sbjct: 133 KDAGVQQCFHRSREYQLGD 151
>SB_977| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 765
Score = 129 bits (312), Expect = 2e-30
Identities = 64/97 (65%), Positives = 74/97 (76%), Gaps = 4/97 (4%)
Frame = +3
Query: 201 RLWR-SGVLWSFQRVPTQR---SAKYFLDDLDRLGAKDYQPTEQDILRTXVKTTGIXEVH 368
RLW SG+ F R + SA Y+L+ LDRLG+ DY PTEQD+LRT VKTTGI E H
Sbjct: 129 RLWSDSGIQACFARSREYQLNDSASYYLNALDRLGSPDYVPTEQDVLRTRVKTTGIVETH 188
Query: 369 FSFKNLNFKLFDVGGQXSERKKWIHCFEDVTAIIFXV 479
F+FK+L+FK+FDVGGQ SERKKWIHCFE VTAIIF V
Sbjct: 189 FTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCV 225
Score = 96.3 bits (229), Expect = 2e-20
Identities = 45/79 (56%), Positives = 59/79 (74%)
Frame = +1
Query: 19 VYSNTIQSLVAILRAMPNLGIIYGNRDRESDGKMVFDVIQRMEDTEPFSEELLAAMKRLW 198
VYSNTIQS++AI+RAM L I +G+ DR D + +F + MED + F+ EL++ MKRLW
Sbjct: 73 VYSNTIQSMIAIIRAMGTLKIEFGHPDRADDAREMFALAGSMEDVK-FTAELISIMKRLW 131
Query: 199 ADSGVQECFGRSNEYQLND 255
+DSG+Q CF RS EYQLND
Sbjct: 132 SDSGIQACFARSREYQLND 150
>SB_49453| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1117
Score = 118 bits (285), Expect = 3e-27
Identities = 58/92 (63%), Positives = 68/92 (73%), Gaps = 1/92 (1%)
Frame = +3
Query: 267 FLDDLDRLGAKDYQPTEQDILRTXVKTTGIXEVHFSFKNLNFKLFDVGGQXSERKKWIHC 446
+LD L R+GA +Y+ TEQD+LRT VKTTGI EV F +K L+FKLFDVGGQ SERKKWIHC
Sbjct: 204 YLDSLQRIGAPNYEATEQDLLRTRVKTTGIVEVQFDYKRLHFKLFDVGGQRSERKKWIHC 263
Query: 447 FEDVTAIIFXVPCLNMXRXC-TG*NTNRMQES 539
FEDVTAII+ V +TNRM+ES
Sbjct: 264 FEDVTAIIYCVALSAYDLTLEEDESTNRMEES 295
>SB_30236| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 353
Score = 104 bits (250), Expect = 6e-23
Identities = 50/96 (52%), Positives = 64/96 (66%), Gaps = 1/96 (1%)
Frame = +3
Query: 255 SAKYFLDDLDRLGAKDYQPTEQDILRTXVKTTGIXEVHFSFKNLNFKLFDVGGQXSERKK 434
SAKY+L DLDRL DY PTEQD+LR T+GI E F + + F++ DVGGQ SER+K
Sbjct: 150 SAKYYLTDLDRLAKPDYLPTEQDVLRARAPTSGIIEYPFDLETIIFRMVDVGGQRSERRK 209
Query: 435 WIHCFEDVTAIIFXVPCLNMXRXCT-G*NTNRMQES 539
WIHCFE+VT+I+F V + + NRM+ES
Sbjct: 210 WIHCFENVTSIMFLVALSEYDQVLVESDSENRMEES 245
Score = 53.6 bits (123), Expect = 1e-07
Identities = 29/79 (36%), Positives = 41/79 (51%)
Frame = +1
Query: 19 VYSNTIQSLVAILRAMPNLGIIYGNRDRESDGKMVFDVIQRMEDTEPFSEELLAAMKRLW 198
VY N I ++ ++ RAM L I Y + + V + T F E A K LW
Sbjct: 73 VYQNIITAMHSLTRAMETLKIPYKDSSNLEKASYIRTVDPKNVTT--FEREYYEATKSLW 130
Query: 199 ADSGVQECFGRSNEYQLND 255
+D+G+QEC+ R EYQL+D
Sbjct: 131 SDAGMQECYDRRREYQLSD 149
>SB_19713| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 507
Score = 91.1 bits (216), Expect = 7e-19
Identities = 46/101 (45%), Positives = 64/101 (63%), Gaps = 4/101 (3%)
Frame = +3
Query: 189 KALGRLWR-SGVLWSFQR---VPTQRSAKYFLDDLDRLGAKDYQPTEQDILRTXVKTTGI 356
+++ RLW +G+ SF R S +YFLD LDR+ DY PT+QD+L + T GI
Sbjct: 337 ESVRRLWTDAGIRDSFDRRREFQLGDSTRYFLDHLDRICRADYIPTKQDMLHSRKATKGI 396
Query: 357 XEVHFSFKNLNFKLFDVGGQXSERKKWIHCFEDVTAIIFXV 479
E F K++ FK+ DVGGQ S+R KW CF++VT+I+F V
Sbjct: 397 VEHEFMIKSIPFKMVDVGGQRSQRAKWFQCFDEVTSILFLV 437
Score = 42.3 bits (95), Expect = 3e-04
Identities = 21/79 (26%), Positives = 38/79 (48%)
Frame = +1
Query: 19 VYSNTIQSLVAILRAMPNLGIIYGNRDRESDGKMVFDVIQRMEDTEPFSEELLAAMKRLW 198
VYSN ++ + + A L I + D E + + + F + +++RLW
Sbjct: 284 VYSNILKGMKVLADARRKLRIDWDAPDNEMHAERILTLQAPPRIDTEFFLQYAESVRRLW 343
Query: 199 ADSGVQECFGRSNEYQLND 255
D+G+++ F R E+QL D
Sbjct: 344 TDAGIRDSFDRRREFQLGD 362
>SB_14105| Best HMM Match : G-alpha (HMM E-Value=0)
Length = 320
Score = 44.0 bits (99), Expect = 1e-04
Identities = 16/27 (59%), Positives = 23/27 (85%)
Frame = +3
Query: 393 KLFDVGGQXSERKKWIHCFEDVTAIIF 473
++ DVGGQ ++RKKWIH F+ VTA++F
Sbjct: 165 QVIDVGGQKNQRKKWIHFFDGVTAVVF 191
>SB_56255| Best HMM Match : Arf (HMM E-Value=0)
Length = 181
Score = 41.9 bits (94), Expect = 5e-04
Identities = 18/49 (36%), Positives = 27/49 (55%)
Frame = +3
Query: 333 TXVKTTGIXEVHFSFKNLNFKLFDVGGQXSERKKWIHCFEDVTAIIFXV 479
T + T G +KN++F ++DVGGQ R W H F++ +IF V
Sbjct: 44 TTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVV 92
>SB_42281| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 179
Score = 41.1 bits (92), Expect = 8e-04
Identities = 17/49 (34%), Positives = 27/49 (55%)
Frame = +3
Query: 333 TXVKTTGIXEVHFSFKNLNFKLFDVGGQXSERKKWIHCFEDVTAIIFXV 479
T + T G +KN++F ++DVGGQ R W H F++ +I+ V
Sbjct: 44 TTIPTIGFNVESVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIYVV 92
>SB_57342| Best HMM Match : Arf (HMM E-Value=0)
Length = 457
Score = 38.7 bits (86), Expect = 0.004
Identities = 16/41 (39%), Positives = 23/41 (56%)
Frame = +3
Query: 378 KNLNFKLFDVGGQXSERKKWIHCFEDVTAIIFXVPCLNMXR 500
KN+ F ++D+GGQ R+ W H F+ IIF V + R
Sbjct: 152 KNITFSVWDIGGQDKIRRLWRHYFQGAEGIIFVVDSADKER 192
>SB_56254| Best HMM Match : Arf (HMM E-Value=5.3e-31)
Length = 650
Score = 38.7 bits (86), Expect = 0.004
Identities = 15/35 (42%), Positives = 23/35 (65%)
Frame = +3
Query: 375 FKNLNFKLFDVGGQXSERKKWIHCFEDVTAIIFXV 479
+KN++F ++DVGGQ R W H F++ +IF V
Sbjct: 507 YKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVV 541
>SB_4844| Best HMM Match : G-alpha (HMM E-Value=3.6e-10)
Length = 290
Score = 38.7 bits (86), Expect = 0.004
Identities = 17/32 (53%), Positives = 20/32 (62%)
Frame = +1
Query: 160 FSEELLAAMKRLWADSGVQECFGRSNEYQLND 255
+ E K LW D+GV EC+ RSNEYQL D
Sbjct: 237 YPPEFYEHTKILWQDAGVLECYERSNEYQLID 268
Score = 32.3 bits (70), Expect = 0.37
Identities = 18/43 (41%), Positives = 27/43 (62%), Gaps = 4/43 (9%)
Frame = +3
Query: 204 LWR-SGVLWSFQRVPTQR---SAKYFLDDLDRLGAKDYQPTEQ 320
LW+ +GVL ++R + A+YFLD +D + +DY PTEQ
Sbjct: 248 LWQDAGVLECYERSNEYQLIDCAQYFLDRVDTIQQQDYLPTEQ 290
>SB_53421| Best HMM Match : Arf (HMM E-Value=0)
Length = 625
Score = 35.9 bits (79), Expect = 0.030
Identities = 19/66 (28%), Positives = 34/66 (51%)
Frame = +3
Query: 303 YQPTEQDILRTXVKTTGIXEVHFSFKNLNFKLFDVGGQXSERKKWIHCFEDVTAIIFXVP 482
Y+ ++++ T V T G ++KN++F ++D+GGQ R W ++ IIF V
Sbjct: 14 YRLKLEEVVST-VPTLGFNVETVTYKNISFTVWDIGGQDKIRALWRVYYQGCQGIIFVVD 72
Query: 483 CLNMXR 500
+ R
Sbjct: 73 SADRER 78
>SB_11310| Best HMM Match : Arf (HMM E-Value=0)
Length = 255
Score = 35.1 bits (77), Expect = 0.052
Identities = 24/91 (26%), Positives = 41/91 (45%), Gaps = 2/91 (2%)
Frame = +3
Query: 213 SGVLWSFQRVPTQRSAKYFLDDLDRLGAKD--YQPTEQDILRTXVKTTGIXEVHFSFKNL 386
+ VL F+R+ + + + LD G Y + + T + T G +KN+
Sbjct: 76 NSVLSLFRRLLGKDELRLLMVGLDAAGKTTILYHLKLDEPVNT-IPTIGFNVEVVEYKNI 134
Query: 387 NFKLFDVGGQXSERKKWIHCFEDVTAIIFXV 479
F ++D+GGQ R W F++ +IF V
Sbjct: 135 KFTVWDIGGQDKIRLLWRLYFQETQGLIFVV 165
>SB_37042| Best HMM Match : Arf (HMM E-Value=0)
Length = 188
Score = 33.9 bits (74), Expect = 0.12
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = +3
Query: 378 KNLNFKLFDVGGQXSERKKWIHCFEDVTAIIFXVPCLNMXR 500
KN+ ++DVGGQ R W H F+ ++F V ++ R
Sbjct: 62 KNVTLTIWDVGGQDKIRPLWRHYFQGTEGLLFVVDSSDVLR 102
>SB_214| Best HMM Match : Arf (HMM E-Value=0)
Length = 148
Score = 33.9 bits (74), Expect = 0.12
Identities = 14/43 (32%), Positives = 20/43 (46%)
Frame = +3
Query: 372 SFKNLNFKLFDVGGQXSERKKWIHCFEDVTAIIFXVPCLNMXR 500
S + F FD+GG R+ W F V I+F + C + R
Sbjct: 16 SMGGMRFTTFDLGGHRQARRIWKDYFPAVNGIVFIIDCADFER 58
>SB_7588| Best HMM Match : DcpS (HMM E-Value=0)
Length = 446
Score = 33.1 bits (72), Expect = 0.21
Identities = 16/38 (42%), Positives = 18/38 (47%)
Frame = +3
Query: 387 NFKLFDVGGQXSERKKWIHCFEDVTAIIFXVPCLNMXR 500
N L DVGGQ R W H F +IF V C + R
Sbjct: 138 NNALLDVGGQDKIRPLWRHYFAGSQGLIFVVDCADRDR 175
>SB_18358| Best HMM Match : Arf (HMM E-Value=0)
Length = 214
Score = 32.3 bits (70), Expect = 0.37
Identities = 19/69 (27%), Positives = 31/69 (44%), Gaps = 2/69 (2%)
Frame = +3
Query: 279 LDRLGAKD--YQPTEQDILRTXVKTTGIXEVHFSFKNLNFKLFDVGGQXSERKKWIHCFE 452
LD+ G Y+ ++ + T T E FK + FK++D+GG+ R W
Sbjct: 29 LDKSGKTSILYRNKFREYVNTVPTTAFNVETIRPFKGIRFKVWDIGGREQNRPLWKAYAR 88
Query: 453 DVTAIIFXV 479
A+I+ V
Sbjct: 89 QTDAVIYVV 97
>SB_4906| Best HMM Match : Trypsin (HMM E-Value=0)
Length = 530
Score = 30.7 bits (66), Expect = 1.1
Identities = 18/45 (40%), Positives = 23/45 (51%)
Frame = -1
Query: 330 LGYLVRWAGNPLLLTGRGRPGNI*RIVELVLVGTTKALLNARVGP 196
+G L + LL T G GN E+ LVGT K ++ A VGP
Sbjct: 108 MGTLNIYNNGKLLFTRSGDHGNRWHFGEVTLVGTRKIVIEAVVGP 152
>SB_16163| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 853
Score = 29.9 bits (64), Expect = 1.9
Identities = 23/89 (25%), Positives = 40/89 (44%)
Frame = +3
Query: 126 RRDSTDGGHGTVQ*GASRRDEKALGRLWRSGVLWSFQRVPTQRSAKYFLDDLDRLGAKDY 305
++D +G H + AS R+E +G + +R+P +K F LD + D
Sbjct: 26 KKDKHNGHHKYKERMASTREEDTVGNQELDDEIERIKRLPEDEFSKIFEKMLDEMNLSD- 84
Query: 306 QPTEQDILRTXVKTTGIXEVHFSFKNLNF 392
+D +R +T + ++ FSFK F
Sbjct: 85 --AHKDPIRKRDMSTKM-DMLFSFKRRQF 110
>SB_5257| Best HMM Match : SlyX (HMM E-Value=2.2)
Length = 641
Score = 28.7 bits (61), Expect = 4.5
Identities = 23/88 (26%), Positives = 42/88 (47%), Gaps = 3/88 (3%)
Frame = +1
Query: 25 SNTIQSLVAILRAMPNLGIIYGNRDRESDGKMV-FDVIQRM-EDTEPFSEELLAAMK-RL 195
+N ++ LV L+ L Y NR+R + + ++ + R+ ++ EPF L A +
Sbjct: 72 ANEVRRLVKQLKECQTLAHTYNNRERLFNMPVTSYEKLGRLIKEFEPFKNMWLTASDWQK 131
Query: 196 WADSGVQECFGRSNEYQLNDPLNISWTT 279
W DS + + + QL +N S+ T
Sbjct: 132 WHDSWMNDPLTTIDAEQLEQNVNNSFKT 159
>SB_2235| Best HMM Match : Arf (HMM E-Value=3.1e-13)
Length = 334
Score = 28.7 bits (61), Expect = 4.5
Identities = 13/41 (31%), Positives = 26/41 (63%)
Frame = +3
Query: 378 KNLNFKLFDVGGQXSERKKWIHCFEDVTAIIFXVPCLNMXR 500
KN++F ++D+GGQ + RK + +D + +I+ V ++ R
Sbjct: 16 KNVHFAVWDIGGQPNLRKHYF--TDDKSGLIYVVDSTDIQR 54
>SB_47990| Best HMM Match : RRM_1 (HMM E-Value=7.8e-28)
Length = 440
Score = 27.9 bits (59), Expect = 7.9
Identities = 16/43 (37%), Positives = 25/43 (58%)
Frame = -3
Query: 301 SFAPNRSRSSRKYLADR*VGTRWNDQSTPERQSRPRAFSSRRE 173
S + RSRSS + + + G+R N QS + +SR R+ S R+
Sbjct: 302 SHSRKRSRSSSRSKSRKKSGSRSNSQSKQKSRSRSRSNSQSRK 344
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,372,613
Number of Sequences: 59808
Number of extensions: 411801
Number of successful extensions: 962
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 915
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 960
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1717720750
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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