Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30072
(723 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) riboso... 143 9e-35
At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) 143 9e-35
At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contai... 143 9e-35
At4g10710.1 68417.m01751 transcriptional regulator-related simil... 31 0.77
At1g29760.1 68414.m03639 expressed protein 31 0.77
At4g38880.1 68417.m05506 amidophosphoribosyltransferase, putativ... 29 2.4
At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase, ... 29 3.1
At3g20840.1 68416.m02635 ovule development protein, putative sim... 28 5.5
At4g08180.3 68417.m01353 oxysterol-binding family protein simila... 27 9.5
At4g08180.2 68417.m01352 oxysterol-binding family protein simila... 27 9.5
At4g08180.1 68417.m01351 oxysterol-binding family protein simila... 27 9.5
>At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) ribosomal
protein S4, Arabidopsis thaliana, PIR:T48480
Length = 262
Score = 143 bits (347), Expect = 9e-35
Identities = 65/84 (77%), Positives = 73/84 (86%)
Frame = +1
Query: 1 ARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEV 180
ARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV
Sbjct: 2 ARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREV 61
Query: 181 LKIVKQRLIKVDGKVRTDPTYPAG 252
+ I+ QR I+VDGKVRTD TYPAG
Sbjct: 62 ISILMQRHIQVDGKVRTDKTYPAG 85
Score = 128 bits (309), Expect = 4e-30
Identities = 73/161 (45%), Positives = 97/161 (60%), Gaps = 5/161 (3%)
Frame = +3
Query: 255 MDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRT 434
MDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+DGRT
Sbjct: 87 MDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRT 146
Query: 435 IRYPDPLIKVNDSIQLDIATTKIMDSSSLSLGTCV*SREAVTWGVWAPSCPA-RDIPAPS 611
IRYPDPLIK ND+I+LD+ KI++ +G V VT G ++
Sbjct: 147 IRYPDPLIKPNDTIKLDLEANKIVEFIKFDVGNVV----MVTGGRNRGRVGVIKNREKHK 202
Query: 612 TLCTSRTPRDTLRHEVEQRVHN----RQGHEAYISLPRGKG 722
+ +D+ HE R+ N +G + ++SLP+GKG
Sbjct: 203 GSFETIHIQDSTGHEFATRLGNVYTIGKGTKPWVSLPKGKG 243
Score = 73.3 bits (172), Expect = 1e-13
Identities = 30/45 (66%), Positives = 38/45 (84%)
Frame = +2
Query: 509 FIKFESGNLCMITGGRNLGRVGTIVSRERHPGSFDIVHIKDSTGH 643
FIKF+ GN+ M+TGGRN GRVG I +RE+H GSF+ +HI+DSTGH
Sbjct: 172 FIKFDVGNVVMVTGGRNRGRVGVIKNREKHKGSFETIHIQDSTGH 216
Score = 32.7 bits (71), Expect = 0.25
Identities = 14/19 (73%), Positives = 15/19 (78%)
Frame = +1
Query: 637 GTHFATRLNNVFIIGKGTK 693
G FATRL NV+ IGKGTK
Sbjct: 215 GHEFATRLGNVYTIGKGTK 233
>At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B)
Length = 262
Score = 143 bits (347), Expect = 9e-35
Identities = 65/84 (77%), Positives = 73/84 (86%)
Frame = +1
Query: 1 ARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEV 180
ARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV
Sbjct: 2 ARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREV 61
Query: 181 LKIVKQRLIKVDGKVRTDPTYPAG 252
+ I+ QR I+VDGKVRTD TYPAG
Sbjct: 62 ISILMQRHIQVDGKVRTDKTYPAG 85
Score = 128 bits (308), Expect = 5e-30
Identities = 73/161 (45%), Positives = 97/161 (60%), Gaps = 5/161 (3%)
Frame = +3
Query: 255 MDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRT 434
MDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+DGRT
Sbjct: 87 MDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRT 146
Query: 435 IRYPDPLIKVNDSIQLDIATTKIMDSSSLSLGTCV*SREAVTWGVWAPSCPA-RDIPAPS 611
IRYPDPLIK ND+I+LD+ KI++ +G V VT G ++
Sbjct: 147 IRYPDPLIKPNDTIKLDLEENKIVEFIKFDVGNVV----MVTGGRNRGRVGVIKNREKHK 202
Query: 612 TLCTSRTPRDTLRHEVEQRVHN----RQGHEAYISLPRGKG 722
+ +D+ HE R+ N +G + ++SLP+GKG
Sbjct: 203 GSFETIHIQDSTGHEFATRLGNVYTIGKGTKPWVSLPKGKG 243
Score = 73.3 bits (172), Expect = 1e-13
Identities = 30/45 (66%), Positives = 38/45 (84%)
Frame = +2
Query: 509 FIKFESGNLCMITGGRNLGRVGTIVSRERHPGSFDIVHIKDSTGH 643
FIKF+ GN+ M+TGGRN GRVG I +RE+H GSF+ +HI+DSTGH
Sbjct: 172 FIKFDVGNVVMVTGGRNRGRVGVIKNREKHKGSFETIHIQDSTGH 216
Score = 32.7 bits (71), Expect = 0.25
Identities = 14/19 (73%), Positives = 15/19 (78%)
Frame = +1
Query: 637 GTHFATRLNNVFIIGKGTK 693
G FATRL NV+ IGKGTK
Sbjct: 215 GHEFATRLGNVYTIGKGTK 233
>At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contains
ribosomal protein S4 signature from residues 8 to 22
Length = 261
Score = 143 bits (347), Expect = 9e-35
Identities = 65/84 (77%), Positives = 73/84 (86%)
Frame = +1
Query: 1 ARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEV 180
ARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV
Sbjct: 2 ARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREV 61
Query: 181 LKIVKQRLIKVDGKVRTDPTYPAG 252
+ I+ QR I+VDGKVRTD TYPAG
Sbjct: 62 ISILMQRHIQVDGKVRTDKTYPAG 85
Score = 128 bits (308), Expect = 5e-30
Identities = 73/161 (45%), Positives = 97/161 (60%), Gaps = 5/161 (3%)
Frame = +3
Query: 255 MDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRT 434
MDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+DGRT
Sbjct: 87 MDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRT 146
Query: 435 IRYPDPLIKVNDSIQLDIATTKIMDSSSLSLGTCV*SREAVTWGVWAPSCPA-RDIPAPS 611
IRYPDPLIK ND+I+LD+ KI++ +G V VT G ++
Sbjct: 147 IRYPDPLIKPNDTIKLDLEENKIVEFIKFDVGNVV----MVTGGRNRGRVGVIKNREKHK 202
Query: 612 TLCTSRTPRDTLRHEVEQRVHN----RQGHEAYISLPRGKG 722
+ +D+ HE R+ N +G + ++SLP+GKG
Sbjct: 203 GSFETIHIQDSTGHEFATRLGNVYTIGKGTKPWVSLPKGKG 243
Score = 73.3 bits (172), Expect = 1e-13
Identities = 30/45 (66%), Positives = 38/45 (84%)
Frame = +2
Query: 509 FIKFESGNLCMITGGRNLGRVGTIVSRERHPGSFDIVHIKDSTGH 643
FIKF+ GN+ M+TGGRN GRVG I +RE+H GSF+ +HI+DSTGH
Sbjct: 172 FIKFDVGNVVMVTGGRNRGRVGVIKNREKHKGSFETIHIQDSTGH 216
Score = 32.7 bits (71), Expect = 0.25
Identities = 14/19 (73%), Positives = 15/19 (78%)
Frame = +1
Query: 637 GTHFATRLNNVFIIGKGTK 693
G FATRL NV+ IGKGTK
Sbjct: 215 GHEFATRLGNVYTIGKGTK 233
>At4g10710.1 68417.m01751 transcriptional regulator-related similar
to chromatin-specific transcription elongation factor
FACT 140 kDa subunit (GI:5499741) [Homo sapiens]
Length = 1074
Score = 31.1 bits (67), Expect = 0.77
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = -2
Query: 122 KHSRNLWGPVDGLGAYTPPSLSNIHAL 42
KHS +LWG D L TPP+ ++ L
Sbjct: 44 KHSADLWGSADALAIATPPASDDLRYL 70
>At1g29760.1 68414.m03639 expressed protein
Length = 526
Score = 31.1 bits (67), Expect = 0.77
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = -2
Query: 233 SVLTFPSTFMRRCFTIFRTSFPVKAYFRRFL 141
S+LTFP +R CF F F + RRFL
Sbjct: 195 SLLTFPPWLLRNCFLFFFDPFSTIRFGRRFL 225
>At4g38880.1 68417.m05506 amidophosphoribosyltransferase, putative /
glutamine phosphoribosylpyrophosphate amidotransferase,
putative / phosphoribosyldiphosphate 5-amidotransferase,
putative similar to amidophosphoribosyltransferase
[Arabidopsis thaliana] GI:469195; contains Pfam profiles
PF00156: Phosphoribosyl transferase domain, PF00310:
Glutamine amidotransferases class-II
Length = 532
Score = 29.5 bits (63), Expect = 2.4
Identities = 18/59 (30%), Positives = 24/59 (40%)
Frame = +2
Query: 473 HPVRHRNYEDYGFIKFESGNLCMITGGRNLGRVGTIVSRERHPGSFDIVHIKDSTGHTS 649
H ++HR E G + L ITG + V T PG I H++ ST S
Sbjct: 97 HALQHRGQEGAGIVAANQNGLESITGVGLVSDVFTESKLNNLPGDIAIGHVRYSTSGAS 155
>At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase,
putative / steroid 5-alpha-reductase, putative identical
to gi:1280611; contains a steroid 5-alpha reductase,
C-terminal domain
Length = 262
Score = 29.1 bits (62), Expect = 3.1
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = -2
Query: 212 TFMRRCFTIFRTSFPVKAYFRRFLRKITRGKHSRNLWGP 96
TF R C + P A +FL+ GKH+R WGP
Sbjct: 8 TFFRYCLLTLIFAGPPTAVLLKFLQA-PYGKHNRTGWGP 45
>At3g20840.1 68416.m02635 ovule development protein, putative
similar to ovule development protein AINTEGUMENTA
(GI:1209099)[Arabidopsis thaliana]
Length = 529
Score = 28.3 bits (60), Expect = 5.5
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = +3
Query: 576 PSCPARDIPAPSTLCTSRTPRDTLRHEVEQRVHNRQ 683
PS + D+ + C S TP ++ HE+++ HN Q
Sbjct: 52 PSVQSNDVVVAA--CDSNTPNNSSYHELQESAHNLQ 85
>At4g08180.3 68417.m01353 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 27.5 bits (58), Expect = 9.5
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +1
Query: 145 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY 243
N ++A+T NE+ +K+RL D ++R D Y
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRY 735
>At4g08180.2 68417.m01352 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 27.5 bits (58), Expect = 9.5
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +1
Query: 145 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY 243
N ++A+T NE+ +K+RL D ++R D Y
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRY 735
>At4g08180.1 68417.m01351 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 814
Score = 27.5 bits (58), Expect = 9.5
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +1
Query: 145 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY 243
N ++A+T NE+ +K+RL D ++R D Y
Sbjct: 704 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRY 736
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,540,465
Number of Sequences: 28952
Number of extensions: 395882
Number of successful extensions: 1180
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 1125
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1180
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1575119672
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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