Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30070
(685 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative / ... 52 3e-07
At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative / ... 49 3e-06
At5g44000.1 68418.m05384 glutathione S-transferase C-terminal do... 36 0.019
At2g33000.1 68415.m04044 ubiquitin-associated (UBA)/TS-N domain-... 36 0.025
At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta id... 31 0.71
At2g18110.1 68415.m02105 elongation factor 1-beta, putative / EF... 30 1.6
At1g49430.1 68414.m05541 long-chain-fatty-acid--CoA ligase / lon... 29 2.2
At5g07270.1 68418.m00829 ankyrin repeat family protein contains ... 29 3.8
At3g49210.1 68416.m05378 expressed protein 28 5.0
At4g18450.1 68417.m02737 ethylene-responsive factor, putative si... 27 8.8
At2g03020.1 68415.m00254 heat shock protein-related Prosite PS00... 27 8.8
>At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative Similar to elongation factor
1-gamma (gb|EF1G_XENLA). ESTs
gb|T20564,gb|T45940,gb|T04527 come from this gene
Length = 414
Score = 52.4 bits (120), Expect = 3e-07
Identities = 29/80 (36%), Positives = 40/80 (50%)
Frame = +1
Query: 7 QWASWSDSELLPASCAWVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTER 186
QW +S E+ + W P +G M ++ E A S L AL L+ HL + T+LV
Sbjct: 96 QWIDFSSLEIYASILRWFGPRMGFMPYSAPAEEGAISTLKRALDALNTHLTSNTYLVGHS 155
Query: 187 ITLADVIVFSTLLHAFQHVL 246
ITLAD+I L F V+
Sbjct: 156 ITLADIITVCNLNLGFATVM 175
Score = 36.7 bits (81), Expect = 0.014
Identities = 16/44 (36%), Positives = 26/44 (59%), Gaps = 3/44 (6%)
Frame = +2
Query: 509 ESKDPFDSMPKGTFNMDDFKRVYSNED---EAKSIPYFWEKFDP 631
++K+P D +P +DD+KR+YSN +I FW+ +DP
Sbjct: 253 KAKNPLDLLPPSPMVLDDWKRLYSNTKSNFREVAIKGFWDMYDP 296
>At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative similar to elongation factor 1B
gamma GI:3868758 from [Oryza sativa]
Length = 413
Score = 49.2 bits (112), Expect = 3e-06
Identities = 27/80 (33%), Positives = 38/80 (47%)
Frame = +1
Query: 7 QWASWSDSELLPASCAWVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTER 186
QW +S E+ W P +G F+ E A S L L+ L+ HL + TFLV
Sbjct: 96 QWIDFSSLEIDANMLKWFAPRMGYAPFSAPAEEAAISALKRGLEALNTHLASNTFLVGHS 155
Query: 187 ITLADVIVFSTLLHAFQHVL 246
+TLAD++ L F V+
Sbjct: 156 VTLADIVTICNLNLGFATVM 175
Score = 36.7 bits (81), Expect = 0.014
Identities = 16/44 (36%), Positives = 26/44 (59%), Gaps = 3/44 (6%)
Frame = +2
Query: 509 ESKDPFDSMPKGTFNMDDFKRVYSNED---EAKSIPYFWEKFDP 631
++K+P D +P +DD+KR+YSN +I FW+ +DP
Sbjct: 252 KAKNPLDLLPPSPMVLDDWKRLYSNTKSNFREVAIKGFWDMYDP 295
>At5g44000.1 68418.m05384 glutathione S-transferase C-terminal
domain-containing protein contains Pfam domain PF00043:
Glutathione S-transferase, C-terminal domain
Length = 399
Score = 36.3 bits (80), Expect = 0.019
Identities = 16/46 (34%), Positives = 31/46 (67%)
Frame = +1
Query: 88 NKQNVERAKSDLLAALKVLDGHLLTRTFLVTERITLADVIVFSTLL 225
+++ + A ++L + L ++ HL + +L ER+TLADV +F+TL+
Sbjct: 261 SQEAYDGAVNELFSTLDEIEDHLGSNRYLCGERLTLADVCLFTTLI 306
>At2g33000.1 68415.m04044 ubiquitin-associated (UBA)/TS-N
domain-containing protein-related contains weak hit to
Pfam F00627: UBA/TS-N domain; supported by tandem
duplication of ubiquitin-associated (UBA)/TS-N domain
protein (GI:2924790) (TIGR_Ath1:At2g33010) [Arabidopsis
thaliana]
Length = 508
Score = 35.9 bits (79), Expect = 0.025
Identities = 24/95 (25%), Positives = 39/95 (41%), Gaps = 2/95 (2%)
Frame = +2
Query: 71 LVSCNSTNRMLNVQSLTYWPP*KYWTDIFSHAPSLLPRESHLPMSLSSVHCCMLSSTCYT 250
L++ + N N +S + WPP K W D H + E+ + S + C +
Sbjct: 195 LMASSQVNTQENQESTSVWPPMKTWVDRICHETKVSREEAFYYLEGSKWNFSRAVEACRS 254
Query: 251 RG-PFV-ADKRSALAPDRRPPATSVGRRRLAHALC 349
+ P V A + SA AP P S+ + + C
Sbjct: 255 KTLPVVSASQESAEAPPNSPEQPSLLKNEWISSFC 289
>At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta
identical to SP|P48006 Elongation factor 1-beta
(EF-1-beta) {Arabidopsis thaliana}
Length = 231
Score = 31.1 bits (67), Expect = 0.71
Identities = 14/32 (43%), Positives = 21/32 (65%)
Frame = +1
Query: 127 AALKVLDGHLLTRTFLVTERITLADVIVFSTL 222
A LK LD HLLTR+++ + + D+ VF+ L
Sbjct: 11 AGLKKLDEHLLTRSYITGYQASKDDITVFAAL 42
>At2g18110.1 68415.m02105 elongation factor 1-beta, putative /
EF-1-beta, putative nearly identical to eEF-1beta
[Arabidopsis thaliana] GI:398606
Length = 231
Score = 29.9 bits (64), Expect = 1.6
Identities = 13/32 (40%), Positives = 21/32 (65%)
Frame = +1
Query: 127 AALKVLDGHLLTRTFLVTERITLADVIVFSTL 222
+ LK LD HLLTR+++ + + D+ VF+ L
Sbjct: 11 SGLKKLDEHLLTRSYITGYQASKDDITVFTAL 42
>At1g49430.1 68414.m05541 long-chain-fatty-acid--CoA ligase /
long-chain acyl-CoA synthetase nearly identical to acyl
CoA synthetase (MF45P) GI:1617268 from [Brassica napus]
Length = 665
Score = 29.5 bits (63), Expect = 2.2
Identities = 16/49 (32%), Positives = 24/49 (48%)
Frame = -3
Query: 290 EPTLNVYQRRTDLGCSTCWKACSSVLKTMTSASVILSVTRKVRVRRCPS 144
E +L Q +T +C K CSS LKT+ S + S ++ +C S
Sbjct: 151 EVSLVFVQEKTVSSILSCQKGCSSNLKTIVSFGEVSSTQKEEAKNQCVS 199
>At5g07270.1 68418.m00829 ankyrin repeat family protein contains
ankyrin repeats, Pfam:PF00023
Length = 513
Score = 28.7 bits (61), Expect = 3.8
Identities = 20/50 (40%), Positives = 23/50 (46%), Gaps = 7/50 (14%)
Frame = +2
Query: 104 NVQSLTYWPP*-------KYWTDIFSHAPSLLPRESHLPMSLSSVHCCML 232
NV S+T PP ++ F PS L RE LPMSL CML
Sbjct: 344 NVPSVTVGPPGSIPCPLCRHGITAFKRLPSSLTREMKLPMSLGFCAPCML 393
>At3g49210.1 68416.m05378 expressed protein
Length = 518
Score = 28.3 bits (60), Expect = 5.0
Identities = 19/82 (23%), Positives = 40/82 (48%)
Frame = +1
Query: 7 QWASWSDSELLPASCAWVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTER 186
+W +W + P S L ++ K+ ++R K+ L AAL + G + +TF V
Sbjct: 361 RWGNWIGYIVFPFSIGLRDDPLQHLRRAKRIIDRKKNSLEAALTFVAGKFILKTFGVQVA 420
Query: 187 ITLADVIVFSTLLHAFQHVLHP 252
+ + + +T + +F +++ P
Sbjct: 421 AKIINRALSNTTM-SFSNLIGP 441
>At4g18450.1 68417.m02737 ethylene-responsive factor, putative
similar to ethylene response factor 1 GB:AAD03544
GI:4128208 from [Arabidopsis thaliana]; EREBP-1
(Ethylene-inducible DNA binding protein that interact
with an ethylene-responsive element) - Nicotiana
tabacum, PATCHX:D1007899
Length = 303
Score = 27.5 bits (58), Expect = 8.8
Identities = 16/51 (31%), Positives = 26/51 (50%), Gaps = 2/51 (3%)
Frame = +2
Query: 107 VQSLTYWPP*KYWTDIFSHAPSLLPRESHLPMSLSSVHCCMLSS--TCYTR 253
+QSL + WT+I S + +H P++ ++ C LSS +C TR
Sbjct: 58 LQSLDMSTEDQEWTEILDAIASFPNKTNHDPLTNPTIDSCSLSSRVSCKTR 108
>At2g03020.1 68415.m00254 heat shock protein-related Prosite
PS00430: TonB-dependent receptor proteins signature 1;
contains some similar to Small heat shock protein,
chloroplast precursor (SP:P30222) {Petunia hybrida}
Length = 255
Score = 27.5 bits (58), Expect = 8.8
Identities = 21/60 (35%), Positives = 28/60 (46%), Gaps = 2/60 (3%)
Frame = -3
Query: 272 YQRRTDLGCSTCWKACSSVLKTMTSA--SVILSVTRKVRVRRCPSSTFKAASRSDFARST 99
YQ T +GCS C A SS+ M+ VILS TR + R P + + + R T
Sbjct: 88 YQTMTGIGCSCC--AISSITTHMSDGVFRVILSKTR-IDPHRSPCTVLGCSGFREDLRGT 144
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,901,074
Number of Sequences: 28952
Number of extensions: 308218
Number of successful extensions: 841
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 819
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 841
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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