Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30061
(781 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g22800.1 68414.m02848 expressed protein similar to Biotin syn... 73 2e-13
At2g41380.1 68415.m05107 embryo-abundant protein-related similar... 37 0.013
At1g73600.1 68414.m08521 phosphoethanolamine N-methyltransferase... 36 0.023
At3g18000.1 68416.m02288 phosphoethanolamine N-methyltransferase... 35 0.053
At3g23260.1 68416.m02932 F-box family protein contains Pfam PF00... 35 0.070
At1g48600.2 68414.m05434 phosphoethanolamine N-methyltransferase... 34 0.12
At1g48600.1 68414.m05435 phosphoethanolamine N-methyltransferase... 34 0.12
At4g34360.1 68417.m04882 protease-related similar to PIR|I46078 ... 30 1.5
At4g27290.1 68417.m03916 S-locus protein kinase, putative simila... 30 1.5
At3g01660.1 68416.m00097 expressed protein similar to putative p... 30 1.5
At2g15760.1 68415.m01804 calmodulin-binding protein similar to A... 29 4.6
At1g69523.1 68414.m07991 UbiE/COQ5 methyltransferase family prot... 29 4.6
At1g29750.2 68414.m03638 leucine-rich repeat transmembrane prote... 29 4.6
At1g29750.1 68414.m03637 leucine-rich repeat transmembrane prote... 29 4.6
At1g08460.1 68414.m00936 histone deacetylase family protein (HDA... 28 6.0
At2g17180.1 68415.m01984 zinc finger (C2H2 type) family protein ... 28 8.0
>At1g22800.1 68414.m02848 expressed protein similar to Biotin
synthesis protein bioC. {Serratia marcescens}
(SP:P36571); ESTs gb|Z34075, gb|Z34835 and gb|AA404888
come from this gene
Length = 355
Score = 72.9 bits (171), Expect = 2e-13
Identities = 36/82 (43%), Positives = 50/82 (60%)
Frame = +2
Query: 509 ELRQALQLSDLERRGGVSPHISPFTRPTDIGGLLNAAGFTLQTVDIDKMTIWYPSAFELM 688
ELR A L+ +ER GG+SP +SP + D G LL AGF+L VD+D+ + Y A +L+
Sbjct: 212 ELRIACTLAHMEREGGISPRLSPLAQVRDAGNLLTRAGFSLPGVDVDEYVVKYKRAMDLI 271
Query: 689 RDLRLAGESNAAHNRSGHLPRD 754
LR GE+NA R+ L R+
Sbjct: 272 EHLRAMGETNALLERNKILNRE 293
Score = 67.7 bits (158), Expect = 8e-12
Identities = 38/108 (35%), Positives = 54/108 (50%), Gaps = 6/108 (5%)
Frame = +3
Query: 201 CRTGSLSWICITAFPAGQVEKVTLCDTSRTHLDKA------IVGEGVQYEKMIMDEENFE 362
C GSL + G +EK+ + DTS + + ++ + DEE
Sbjct: 103 CLGGSLGAVKRLLRGRGGIEKLIMMDTSYDMIKSCRDAQDDSLDNSIETSYFVGDEEFLP 162
Query: 363 LPDDSVDLLVSSLTFHWVNDLPGLFDRIMKCLKPDGVLLACTFGGDTL 506
+ + SVDL++SSL HW NDLPG + LKPDG+ LA GG+TL
Sbjct: 163 VKESSVDLIISSLGLHWTNDLPGSMIQCKLALKPDGLFLAAILGGETL 210
Score = 36.7 bits (81), Expect = 0.017
Identities = 22/62 (35%), Positives = 31/62 (50%)
Frame = +1
Query: 52 MNIFDRKTKMIQRERAACNDEYHLSEYVKEEIGWRTADKIFDIKRTFKNAVELGASRGYV 231
+ IFDR K I R+RAA +V + + D++ D K++F A LG S G V
Sbjct: 53 VKIFDRDLKRIHRDRAAWLSRQKNDSFV-DAVADNLLDRLEDCKKSFPTAFCLGGSLGAV 111
Query: 232 SR 237
R
Sbjct: 112 KR 113
>At2g41380.1 68415.m05107 embryo-abundant protein-related similar to
embryo-abundant protein [Picea glauca] GI:1350531
Length = 269
Score = 37.1 bits (82), Expect = 0.013
Identities = 29/85 (34%), Positives = 39/85 (45%), Gaps = 6/85 (7%)
Frame = +3
Query: 246 AGQVEKVTLCDTSRTHLDKAIVGEGVQYE----KMIMDE-ENFELPDDSVDLLVSSLTFH 410
AG + V DTS L+ A V+YE M E E P+ SVDL+ + H
Sbjct: 52 AGIYKNVIATDTSSKQLEFAAKLPNVRYEITPPTMSSSEIEKLVAPESSVDLVTVAQALH 111
Query: 411 WVNDLPGLFDRIMKCL-KPDGVLLA 482
W DL + + L KP+GV+ A
Sbjct: 112 WF-DLTNFYSNVKHVLKKPNGVIAA 135
>At1g73600.1 68414.m08521 phosphoethanolamine N-methyltransferase 3,
putative (NMT3) strong similarity to SP|Q9FR44
Phosphoethanolamine N-methyltransferase 1 (EC 2.1.1.103)
(PEAMT 1) (AtNMT1) {Arabidopsis thaliana}; identical to
SP|Q9C6B9 Putative phosphoethanolamine
N-methyltransferase 3 {Arabidopsis thaliana}
Length = 555
Score = 36.3 bits (80), Expect = 0.023
Identities = 20/57 (35%), Positives = 28/57 (49%)
Frame = +3
Query: 309 VGEGVQYEKMIMDEENFELPDDSVDLLVSSLTFHWVNDLPGLFDRIMKCLKPDGVLL 479
+G E + D E PD++ D++ S T + D P LF R K LKP G +L
Sbjct: 390 IGLKCSVEFEVADCTKKEYPDNTFDVIYSRDTILHIQDKPALFRRFYKWLKPGGKVL 446
>At3g18000.1 68416.m02288 phosphoethanolamine N-methyltransferase 1
/ PEAMT 1 (NMT1) identical to Phosphoethanolamine
N-methyltransferase 1 (EC 2.1.1.103) (PEAMT 1) (AtNMT1)
(SP:Q9FR44){Arabidopsis thaliana}; strong similarity to
phosphoethanolamine N-methyltransferase from [Spinacia
oleracea] GI:7407189, [Triticum aestivum] GI:17887465;
contains Pfam profile PF01209: methlytransferase,
UbiE/COQ5 family
Length = 491
Score = 35.1 bits (77), Expect = 0.053
Identities = 22/62 (35%), Positives = 30/62 (48%)
Frame = +3
Query: 294 LDKAIVGEGVQYEKMIMDEENFELPDDSVDLLVSSLTFHWVNDLPGLFDRIMKCLKPDGV 473
L++AI G E + D PD+S D++ S T + D P LF K LKP G
Sbjct: 322 LERAI-GLSCSVEFEVADCTTKHYPDNSFDVIYSRDTILHIQDKPALFRTFFKWLKPGGK 380
Query: 474 LL 479
+L
Sbjct: 381 VL 382
>At3g23260.1 68416.m02932 F-box family protein contains Pfam
PF00646: F-box domain; contains TIGRFAM TIGR01640: F-box
protein interaction domain
Length = 362
Score = 34.7 bits (76), Expect = 0.070
Identities = 18/63 (28%), Positives = 33/63 (52%)
Frame = -2
Query: 348 HPLSSFHIERPHQLLPYRDEFWMCHTVSPSPPVRQEMP*YISTRGSQFYSVFERSFNVEY 169
H F IE +++ + ++ W H + +RQ+ P +S +GS ++ V R F +Y
Sbjct: 164 HVQGDFKIE--YEIYDFTNDSWRVHGATTELSIRQKHP--VSVKGSTYWVVRNRYFPYKY 219
Query: 168 FVS 160
F+S
Sbjct: 220 FLS 222
>At1g48600.2 68414.m05434 phosphoethanolamine N-methyltransferase 2,
putative (NMT2) very similar to |PEM2_ARATH Putative
phosphoethanolamine N-methyltransferase 2 (EC 2.1.1.103)
(SP:Q944H0){Arabidopsis thaliana}; very similar to
Halotolerance protein Hal3b (SP:P94063)[Arabidopsis
thaliana]; to similar to GB:AAF61950 from [Spinacia
oleracea]
Length = 491
Score = 33.9 bits (74), Expect = 0.12
Identities = 22/62 (35%), Positives = 30/62 (48%)
Frame = +3
Query: 294 LDKAIVGEGVQYEKMIMDEENFELPDDSVDLLVSSLTFHWVNDLPGLFDRIMKCLKPDGV 473
L++AI G E + D PD+S D++ S T + D P LF K LKP G
Sbjct: 322 LERAI-GLKCSVEFEVADCTTKTYPDNSFDVIYSRDTILHIQDKPALFRTFFKWLKPGGK 380
Query: 474 LL 479
+L
Sbjct: 381 VL 382
>At1g48600.1 68414.m05435 phosphoethanolamine N-methyltransferase 2,
putative (NMT2) very similar to |PEM2_ARATH Putative
phosphoethanolamine N-methyltransferase 2 (EC 2.1.1.103)
(SP:Q944H0){Arabidopsis thaliana}; very similar to
Halotolerance protein Hal3b (SP:P94063)[Arabidopsis
thaliana]; to similar to GB:AAF61950 from [Spinacia
oleracea]
Length = 475
Score = 33.9 bits (74), Expect = 0.12
Identities = 22/62 (35%), Positives = 30/62 (48%)
Frame = +3
Query: 294 LDKAIVGEGVQYEKMIMDEENFELPDDSVDLLVSSLTFHWVNDLPGLFDRIMKCLKPDGV 473
L++AI G E + D PD+S D++ S T + D P LF K LKP G
Sbjct: 306 LERAI-GLKCSVEFEVADCTTKTYPDNSFDVIYSRDTILHIQDKPALFRTFFKWLKPGGK 364
Query: 474 LL 479
+L
Sbjct: 365 VL 366
>At4g34360.1 68417.m04882 protease-related similar to PIR|I46078
endothelin converting enzyme, Bos primigenius taurus
Length = 248
Score = 30.3 bits (65), Expect = 1.5
Identities = 14/46 (30%), Positives = 23/46 (50%)
Frame = +3
Query: 414 VNDLPGLFDRIMKCLKPDGVLLACTFGGDTLMSCVRRSNCLTWSGE 551
V+ + D + + LKPDG+ ++ TFG + + TWS E
Sbjct: 145 VSKVMATLDGVHRVLKPDGIFISITFGQPHFRRPLFKDPKFTWSME 190
>At4g27290.1 68417.m03916 S-locus protein kinase, putative similar
to S-receptor kinase gi|392557|gb|AAA62232; contains
Pfam domains PF00954: S-locus glycoprotein family,
PF00069: Protein kinase domain and PF01453: Lectin
(probable mannose binding)
Length = 772
Score = 30.3 bits (65), Expect = 1.5
Identities = 19/49 (38%), Positives = 24/49 (48%), Gaps = 1/49 (2%)
Frame = +3
Query: 495 GDTLMSCVRRSNCLTWSGEEGFLLIYH-RLPDPRTLGVCSMLLVLRCKQ 638
GD CVRR GE+GFL I +LPD RT + + CK+
Sbjct: 317 GDWSEGCVRRVKLDCGKGEDGFLKISKLKLPDTRTSWYDKNMDLNECKK 365
>At3g01660.1 68416.m00097 expressed protein similar to putative
protein GB:CAB45319 [Arabidopsis thaliana]
Length = 273
Score = 30.3 bits (65), Expect = 1.5
Identities = 13/51 (25%), Positives = 27/51 (52%)
Frame = +3
Query: 327 YEKMIMDEENFELPDDSVDLLVSSLTFHWVNDLPGLFDRIMKCLKPDGVLL 479
+ K + +++ FE D D ++ S+ ++ +F + + LKP GVL+
Sbjct: 138 FVKDLNEDQKFEFEDKYFDAVLCSVGVQYLQQPEKVFAEVYRVLKPGGVLI 188
>At2g15760.1 68415.m01804 calmodulin-binding protein similar to
AR781 GI:1669593 from [Arabidopsis thaliana]; AR781
complements pheromone receptor deficient mutant of
Shizosaccharomyces pombe
Length = 315
Score = 28.7 bits (61), Expect = 4.6
Identities = 23/69 (33%), Positives = 35/69 (50%), Gaps = 4/69 (5%)
Frame = -1
Query: 589 GSGKR*YMRRNPSS--PLQVRQLERLTQLIRVSPPKVQASNTPSGLRHFIILSKS--PGR 422
GS Y R+ S PL+V + + + V K+ ASNT S + + LS PGR
Sbjct: 137 GSSSSRYDRKGSRSMSPLRVSDI-MVDEEEEVQSTKMVASNT-SNQKSSVFLSAILFPGR 194
Query: 421 SFTQWKVSD 395
++ +WK+ D
Sbjct: 195 AYKKWKLKD 203
>At1g69523.1 68414.m07991 UbiE/COQ5 methyltransferase family protein
low similarity to SP|Q05197 Phosphatidylethanolamine
N-methyltransferase (EC 2.1.1.17) [Rhodopseudomonas
sphaeroides] {Rhodobacter sphaeroides}; contains Pfam
profile PF01209: methlytransferase, UbiE/COQ5 family
Length = 300
Score = 28.7 bits (61), Expect = 4.6
Identities = 14/43 (32%), Positives = 22/43 (51%)
Frame = +3
Query: 351 ENFELPDDSVDLLVSSLTFHWVNDLPGLFDRIMKCLKPDGVLL 479
E+ L D SVD +V +L V D+ I + L+P G+ +
Sbjct: 185 ESIPLEDASVDAVVGTLVLCSVTDVTQTLKEIKRILRPGGIYI 227
>At1g29750.2 68414.m03638 leucine-rich repeat transmembrane protein
kinase, putative / serine/threonine kinase, putative
(RKF1) similar to receptor-like serine/threonine kinase
GI:2465923 from [Arabidopsis thaliana]; identical to cDNA
receptor-like serine/threonine kinase (RKF1) GI:2465922
Length = 1021
Score = 28.7 bits (61), Expect = 4.6
Identities = 16/63 (25%), Positives = 31/63 (49%)
Frame = -3
Query: 326 LNALTNYCLIEMSSGCVTQCHLLHLSGRKCRDTYPREAPSSTAFLNVLLMSNILSAVRQP 147
+ A + CL+E ++ CV HL+ + + R R+ + A + V L+ + S +P
Sbjct: 886 MGAGDSVCLLEFANECVESGHLMQVVDERLRPEVDRK--EAEAVIKVALVCSSASPTDRP 943
Query: 146 ISS 138
+ S
Sbjct: 944 LMS 946
>At1g29750.1 68414.m03637 leucine-rich repeat transmembrane protein
kinase, putative / serine/threonine kinase, putative
(RKF1) similar to receptor-like serine/threonine kinase
GI:2465923 from [Arabidopsis thaliana]; identical to cDNA
receptor-like serine/threonine kinase (RKF1) GI:2465922
Length = 1006
Score = 28.7 bits (61), Expect = 4.6
Identities = 16/63 (25%), Positives = 31/63 (49%)
Frame = -3
Query: 326 LNALTNYCLIEMSSGCVTQCHLLHLSGRKCRDTYPREAPSSTAFLNVLLMSNILSAVRQP 147
+ A + CL+E ++ CV HL+ + + R R+ + A + V L+ + S +P
Sbjct: 871 MGAGDSVCLLEFANECVESGHLMQVVDERLRPEVDRK--EAEAVIKVALVCSSASPTDRP 928
Query: 146 ISS 138
+ S
Sbjct: 929 LMS 931
>At1g08460.1 68414.m00936 histone deacetylase family protein (HDA8)
identical to HDA8 [Arabidopsis thaliana] GI:21360988low
similarity to SP|Q9Z2V5 Histone deacetylase 6 (HD6)
(Histone deacetylase mHDA2) {Mus musculus}; contains
Pfam profile PF00850: Histone deacetylase family;
supporting cDNA gi|21360987|gb|AF510167.1|
Length = 377
Score = 28.3 bits (60), Expect = 6.0
Identities = 15/55 (27%), Positives = 30/55 (54%), Gaps = 3/55 (5%)
Frame = +2
Query: 497 RHPDELRQALQLSDLERRGGVSPHISPFT-RPTDIGGLL--NAAGFTLQTVDIDK 652
+HP+ + + + RRG ++PH++ FT P + LL + + + + V+ DK
Sbjct: 38 KHPENADRVRNMLSILRRGPIAPHVNWFTGLPAIVSELLMFHTSEYIEKLVEADK 92
>At2g17180.1 68415.m01984 zinc finger (C2H2 type) family protein
contains Pfam domain, PF00096: Zinc finger, C2H2 type
Length = 270
Score = 27.9 bits (59), Expect = 8.0
Identities = 12/25 (48%), Positives = 16/25 (64%), Gaps = 1/25 (4%)
Frame = -2
Query: 744 RC-PERLWAALLSPANLRSRISSKA 673
RC PER W + P+N + RI+S A
Sbjct: 85 RCHPERQWRGINPPSNFKRRINSNA 109
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,418,727
Number of Sequences: 28952
Number of extensions: 414510
Number of successful extensions: 1244
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 1203
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1244
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1746037600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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