Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30142
         (606 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p...   132   2e-31
At1g06220.2 68414.m00656 elongation factor Tu family protein sim...   109   2e-24
At1g06220.1 68414.m00655 elongation factor Tu family protein sim...   109   2e-24
At5g25230.1 68418.m02991 elongation factor Tu family protein tra...   106   1e-23
At3g22980.1 68416.m02898 elongation factor Tu family protein sim...    79   2e-15
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati...    44   1e-04
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati...    44   1e-04
At1g62750.1 68414.m07082 elongation factor Tu family protein sim...    41   6e-04
At5g13650.2 68418.m01585 elongation factor family protein contai...    38   0.004
At5g13650.1 68418.m01584 elongation factor family protein contai...    38   0.004
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-...    31   0.78 
At1g78910.1 68414.m09199 pseudouridine synthase family protein l...    28   4.2  
At3g63180.1 68416.m07097 expressed protein                             28   5.5  
At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein /...    27   7.3  
At5g41320.1 68418.m05022 expressed protein                             27   9.6  

>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
           putative similar to ELONGATION FACTOR 2 GB:O14460 from
           [Schizosaccharomyces pombe]
          Length = 843

 Score =  132 bits (319), Expect = 2e-31
 Identities = 56/85 (65%), Positives = 70/85 (82%)
 Frame = +2

Query: 2   EPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLR 181
           EPVY+ EIQ PE A+GGIY VLN++RGHVFEE Q  GTP++ +KAYLPV ESFGF++ LR
Sbjct: 727 EPVYMVEIQAPEGALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVVESFGFSSQLR 786

Query: 182 SNTGGQAFPQCVFDHWQVLPGDPCD 256
           + T GQAFPQCVFDHW+++  DP +
Sbjct: 787 AATSGQAFPQCVFDHWEMMSSDPLE 811


>At1g06220.2 68414.m00656 elongation factor Tu family protein similar
            to Cryptosporidium parvum elongation factor-2 GB:U21667
            GI:706974 from [Cryptosporidium parvum]
          Length = 987

 Score =  109 bits (261), Expect = 2e-24
 Identities = 52/85 (61%), Positives = 59/85 (69%)
 Frame = +2

Query: 2    EPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLR 181
            EPVY  EIQ P   V  IY VL+RRRGHV  +    GTP +IVKA+LPV ESFGF  DLR
Sbjct: 843  EPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFGFETDLR 902

Query: 182  SNTGGQAFPQCVFDHWQVLPGDPCD 256
             +T GQAF   VFDHW ++PGDP D
Sbjct: 903  YHTQGQAFCLSVFDHWAIVPGDPLD 927


>At1g06220.1 68414.m00655 elongation factor Tu family protein similar
            to Cryptosporidium parvum elongation factor-2 GB:U21667
            GI:706974 from [Cryptosporidium parvum]
          Length = 987

 Score =  109 bits (261), Expect = 2e-24
 Identities = 52/85 (61%), Positives = 59/85 (69%)
 Frame = +2

Query: 2    EPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLR 181
            EPVY  EIQ P   V  IY VL+RRRGHV  +    GTP +IVKA+LPV ESFGF  DLR
Sbjct: 843  EPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFGFETDLR 902

Query: 182  SNTGGQAFPQCVFDHWQVLPGDPCD 256
             +T GQAF   VFDHW ++PGDP D
Sbjct: 903  YHTQGQAFCLSVFDHWAIVPGDPLD 927


>At5g25230.1 68418.m02991 elongation factor Tu family protein
            translation Elongation Factor 2, Schizosaccharomyces
            pombe, PIR:T39902
          Length = 973

 Score =  106 bits (255), Expect = 1e-23
 Identities = 51/85 (60%), Positives = 59/85 (69%)
 Frame = +2

Query: 2    EPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLR 181
            EPVY  EIQ P   V  IY VL+RRRG+V  +    GTP +IVKA+LPV ESFGF  DLR
Sbjct: 829  EPVYYVEIQTPIDCVTAIYTVLSRRRGYVTSDVPQPGTPAYIVKAFLPVIESFGFETDLR 888

Query: 182  SNTGGQAFPQCVFDHWQVLPGDPCD 256
             +T GQAF   VFDHW ++PGDP D
Sbjct: 889  YHTQGQAFCLSVFDHWAIVPGDPLD 913


>At3g22980.1 68416.m02898 elongation factor Tu family protein similar
            to eukaryotic translation elongation factor 2
            GB:NP_001952 [Homo sapiens]
          Length = 1015

 Score = 79.4 bits (187), Expect = 2e-15
 Identities = 35/83 (42%), Positives = 49/83 (59%)
 Frame = +2

Query: 2    EPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLR 181
            E +Y CE+      +G +Y VL+RRR  + +E    G+ +F V AY+PV+ESFGF  +LR
Sbjct: 876  EAMYFCELNTAPEYLGPMYAVLSRRRARILKEEMQEGSSLFTVHAYVPVSESFGFADELR 935

Query: 182  SNTGGQAFPQCVFDHWQVLPGDP 250
              T G A    V  HW++L  DP
Sbjct: 936  KGTSGGASALMVLSHWEMLEEDP 958


>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
           similar to SP|P25039 Elongation factor G 1,
           mitochondrial precursor (mEF-G-1) {Saccharomyces
           cerevisiae}; contains Pfam profiles PF00009: Elongation
           factor Tu GTP binding domain, PF03764: Elongation factor
           G domain IV, PF00679: Elongation factor G C-terminus
          Length = 754

 Score = 43.6 bits (98), Expect = 1e-04
 Identities = 22/66 (33%), Positives = 36/66 (54%)
 Frame = +2

Query: 2   EPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLR 181
           EPV L E++ P    G + G +N+R+G +    Q       ++ A +P+N  FG++  LR
Sbjct: 659 EPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVPLNNMFGYSTSLR 716

Query: 182 SNTGGQ 199
           S T G+
Sbjct: 717 SMTQGK 722


>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
           similar to mitochondrial elongation factor GI:3917 from
           [Saccharomyces cerevisiae]
          Length = 754

 Score = 43.6 bits (98), Expect = 1e-04
 Identities = 22/66 (33%), Positives = 36/66 (54%)
 Frame = +2

Query: 2   EPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLR 181
           EPV L E++ P    G + G +N+R+G +    Q       ++ A +P+N  FG++  LR
Sbjct: 659 EPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVPLNNMFGYSTSLR 716

Query: 182 SNTGGQ 199
           S T G+
Sbjct: 717 SMTQGK 722


>At1g62750.1 68414.m07082 elongation factor Tu family protein
           similar to elongation factor G SP:P34811 [Glycine max
           (Soybean)]
          Length = 783

 Score = 41.1 bits (92), Expect = 6e-04
 Identities = 24/80 (30%), Positives = 39/80 (48%)
 Frame = +2

Query: 2   EPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLR 181
           EP+   E+  PE  +G + G LN RRG +       G  + +V + +P+ E F + + LR
Sbjct: 689 EPIMRVEVVTPEEHLGDVIGDLNSRRGQINSFGDKPG-GLKVVDSLVPLAEMFQYVSTLR 747

Query: 182 SNTGGQAFPQCVFDHWQVLP 241
             T G+A        + V+P
Sbjct: 748 GMTKGRASYTMQLAKFDVVP 767


>At5g13650.2 68418.m01585 elongation factor family protein contains
           Pfam profiles: PF00009 elongation factor Tu GTP binding
           domain,PF00679 elongation factor G C-terminus,  PF03144
           elongation factor Tu domain 2
          Length = 676

 Score = 38.3 bits (85), Expect = 0.004
 Identities = 20/82 (24%), Positives = 36/82 (43%)
 Frame = +2

Query: 2   EPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLR 181
           EP  +  ++ PE  +G +  +L +RRG +F+   V       ++  +P     G    + 
Sbjct: 476 EPYEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGLRNAIL 535

Query: 182 SNTGGQAFPQCVFDHWQVLPGD 247
           + + G A    VFD +    GD
Sbjct: 536 TASRGTAILNTVFDSYGPWAGD 557


>At5g13650.1 68418.m01584 elongation factor family protein contains
           Pfam profiles: PF00009 elongation factor Tu GTP binding
           domain,PF00679 elongation factor G C-terminus,  PF03144
           elongation factor Tu domain 2
          Length = 675

 Score = 38.3 bits (85), Expect = 0.004
 Identities = 20/82 (24%), Positives = 36/82 (43%)
 Frame = +2

Query: 2   EPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLR 181
           EP  +  ++ PE  +G +  +L +RRG +F+   V       ++  +P     G    + 
Sbjct: 475 EPYEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGLRNAIL 534

Query: 182 SNTGGQAFPQCVFDHWQVLPGD 247
           + + G A    VFD +    GD
Sbjct: 535 TASRGTAILNTVFDSYGPWAGD 556


>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
           GTP-binding protein GUF1 - Saccharomyces cerevisiae,
           PIR:S50374
          Length = 661

 Score = 30.7 bits (66), Expect = 0.78
 Identities = 21/68 (30%), Positives = 32/68 (47%), Gaps = 1/68 (1%)
 Frame = +2

Query: 2   EPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNE-SFGFTADL 178
           EP  +  I  P   VG +  + + RRG   E + +    +F+ K  LP+ E    F  +L
Sbjct: 466 EPTVIATIILPSEYVGAVINLCSDRRGQQLEYTFIDAQRVFL-KYQLPLREIVVDFYDEL 524

Query: 179 RSNTGGQA 202
           +S T G A
Sbjct: 525 KSITSGYA 532


>At1g78910.1 68414.m09199 pseudouridine synthase family protein low
           similarity to SP|P23851 Ribosomal large subunit
           pseudouridine synthase C (EC 4.2.1.70) (Pseudouridylate
           synthase) (Uracil hydrolyase) {Escherichia coli};
           contains Pfam profile PF00849: RNA pseudouridylate
           synthase
          Length = 478

 Score = 28.3 bits (60), Expect = 4.2
 Identities = 22/65 (33%), Positives = 31/65 (47%), Gaps = 3/65 (4%)
 Frame = +1

Query: 76  SWSRFRRVPGGRYTY-VHC-EGLPTCQ*VVRFYCRFAFQHRR-TGLPAVRIRPLAGPPWR 246
           SW   R +   ++   VHC E L T   +V  Y    F H+R   +P V I P  G P++
Sbjct: 340 SWVELRPITSRKHQLRVHCAEALGTP--IVGDYKYGWFVHKRWKQMPQVDIEPTTGKPYK 397

Query: 247 PVRPQ 261
             RP+
Sbjct: 398 LRRPE 402


>At3g63180.1 68416.m07097 expressed protein
          Length = 978

 Score = 27.9 bits (59), Expect = 5.5
 Identities = 12/45 (26%), Positives = 23/45 (51%)
 Frame = +3

Query: 135 PTYLSMSRSVLLPICVPTPADRPSRSAYSTIGRSSLETRATSEQA 269
           P ++  +   L P+ VP+ + RP++S +  +G +S      S  A
Sbjct: 539 PAFIYPANHHLQPVMVPSKSSRPTKSPHLAVGLASANFSHPSSSA 583


>At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein /
           polygalacturonase (pectinase) family protein weak
           similarity to polygalacturonase PG1 [Glycine max]
           GI:5669846; contains PF00295: Glycosyl hydrolases family
           28
          Length = 444

 Score = 27.5 bits (58), Expect = 7.3
 Identities = 11/22 (50%), Positives = 17/22 (77%), Gaps = 1/22 (4%)
 Frame = +1

Query: 76  SWSRFRRVPGGRY-TYVHCEGL 138
           S+ R R +PGGRY +++H +GL
Sbjct: 123 SYGRGRELPGGRYMSFIHGDGL 144


>At5g41320.1 68418.m05022 expressed protein 
          Length = 515

 Score = 27.1 bits (57), Expect = 9.6
 Identities = 16/49 (32%), Positives = 25/49 (51%), Gaps = 2/49 (4%)
 Frame = +3

Query: 177 CVPTPADRPSRSAYSTIGRSSLETRATSEQAL--QRCTGNEKEERIEGR 317
           C   P DR   S  S +GR+  + +  S++AL  Q    N K E ++G+
Sbjct: 253 CNGGPLDRFLHSGQSGLGRNYFQQQQESQEALVAQTEMDNSKNETVKGQ 301


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,352,922
Number of Sequences: 28952
Number of extensions: 254348
Number of successful extensions: 631
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 614
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 629
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1206913392
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)