Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30036
(699 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g04840.1 68416.m00525 40S ribosomal protein S3A (RPS3aA) simi... 125 2e-29
At4g34670.1 68417.m04922 40S ribosomal protein S3A (RPS3aB) 124 6e-29
At4g37440.1 68417.m05299 expressed protein 33 0.18
At3g17380.1 68416.m02221 meprin and TRAF homology domain-contain... 32 0.42
At2g46530.1 68415.m05802 transcriptional factor B3 family protei... 31 0.56
At3g52640.1 68416.m05799 nicastrin-related contains weak similar... 30 1.3
At1g74390.1 68414.m08618 exonuclease family protein contains exo... 29 3.9
At5g42470.1 68418.m05170 expressed protein low similarity to BRE... 28 5.2
At2g17930.1 68415.m02076 FAT domain-containing protein / phospha... 28 6.8
At4g00890.1 68417.m00120 proline-rich family protein contains pr... 27 9.0
>At3g04840.1 68416.m00525 40S ribosomal protein S3A (RPS3aA) similar
to 40S ribosomal protein S3A (S phase specific protein
GBIS289) GB:P49396 [Brassica rapa]
Length = 262
Score = 125 bits (302), Expect = 2e-29
Identities = 65/102 (63%), Positives = 77/102 (75%), Gaps = 2/102 (1%)
Frame = +2
Query: 2 KKGVKKKIVDPFTRKDWYDVKAPSMFSKRQVGTTLVNRTQGTKIASEGLKGRVFEVSLAD 181
+KG KKK VDPF++KDWYDVKAPS+F+ R VG TLV+RTQGTKIASEGLK RVFEVSLAD
Sbjct: 13 RKGGKKKAVDPFSKKDWYDVKAPSIFTHRNVGKTLVSRTQGTKIASEGLKHRVFEVSLAD 72
Query: 182 LQADTDAERSFRKFRLIAEYVQGR--MCSATSTAWTSQPISS 301
LQ D D ++RK RL AE VQGR +C +T+ + S
Sbjct: 73 LQGDED--NAYRKIRLRAEDVQGRNVLCQFWGMDFTTDKLRS 112
Score = 124 bits (299), Expect = 6e-29
Identities = 62/122 (50%), Positives = 83/122 (68%)
Frame = +1
Query: 256 VLCNFHGMDLTTDKLRWMVKKWQTLIEANIDVKTTDGYVLRVFCIGFTNKDSLSQRKTCY 435
VLC F GMD TTDKLR +VKKWQTLIEA++DVKTTD Y LR+FCI FT + + ++TCY
Sbjct: 96 VLCQFWGMDFTTDKLRSLVKKWQTLIEAHVDVKTTDSYTLRLFCIAFTKRRANQVKRTCY 155
Query: 436 AQHTQVRAIRKKMCEIITRDVTNSDSGRW*TS*FPDSIAKDIEKGLPWHLTLCAMFGIRK 615
AQ +Q+R IR+KM +I+ R+ ++ D P++I ++IEK L +F IRK
Sbjct: 156 AQSSQIRQIRRKMRDIMVREASSCDLKDLVAKFIPEAIGREIEKATQGIYPLQNVF-IRK 214
Query: 616 *K 621
K
Sbjct: 215 VK 216
>At4g34670.1 68417.m04922 40S ribosomal protein S3A (RPS3aB)
Length = 262
Score = 124 bits (299), Expect = 6e-29
Identities = 62/122 (50%), Positives = 83/122 (68%)
Frame = +1
Query: 256 VLCNFHGMDLTTDKLRWMVKKWQTLIEANIDVKTTDGYVLRVFCIGFTNKDSLSQRKTCY 435
VL F GMD TTDKLR +VKKWQTLIEA++DVKTTDGY LR+FCI FT + + ++TCY
Sbjct: 96 VLTQFWGMDFTTDKLRSLVKKWQTLIEAHVDVKTTDGYTLRMFCIAFTKRRANQVKRTCY 155
Query: 436 AQHTQVRAIRKKMCEIITRDVTNSDSGRW*TS*FPDSIAKDIEKGLPWHLTLCAMFGIRK 615
AQ +Q+R IR+KM EI+ ++ ++ D P++I ++IEK L +F IRK
Sbjct: 156 AQSSQIRQIRRKMSEIMVKEASSCDLKELVAKFIPEAIGREIEKATQGIYPLQNVF-IRK 214
Query: 616 *K 621
K
Sbjct: 215 VK 216
Score = 122 bits (293), Expect = 3e-28
Identities = 61/84 (72%), Positives = 68/84 (80%)
Frame = +2
Query: 2 KKGVKKKIVDPFTRKDWYDVKAPSMFSKRQVGTTLVNRTQGTKIASEGLKGRVFEVSLAD 181
+KG KKK VDPF++KDWYDVKAP F+ R VG TLV+RTQGTKIASEGLK RVFEVSLAD
Sbjct: 13 RKGGKKKAVDPFSKKDWYDVKAPGSFTNRNVGKTLVSRTQGTKIASEGLKHRVFEVSLAD 72
Query: 182 LQADTDAERSFRKFRLIAEYVQGR 253
LQ D D ++RK RL AE VQGR
Sbjct: 73 LQNDED--NAYRKIRLRAEDVQGR 94
>At4g37440.1 68417.m05299 expressed protein
Length = 471
Score = 33.1 bits (72), Expect = 0.18
Identities = 20/60 (33%), Positives = 30/60 (50%), Gaps = 1/60 (1%)
Frame = +2
Query: 461 SERKCVKSLHATSLTLTQGGGEQVDFL-TPLPRTSRRACHGILPSARCLASESESVEEGP 637
SE K VKS +S ++ E D L + + + RR I+P + +E S+EEGP
Sbjct: 349 SEEKPVKSASVSSHHVSPEDDETTDILLSEILASKRREGKSIIPDKNLVKTEQASIEEGP 408
>At3g17380.1 68416.m02221 meprin and TRAF homology domain-containing
protein / MATH domain-containing protein similar to
ubiquitin-specific protease 12 [Arabidopsis thaliana]
GI:11993471; contains Pfam profile PF00917: MATH domain
Length = 309
Score = 31.9 bits (69), Expect = 0.42
Identities = 16/51 (31%), Positives = 28/51 (54%), Gaps = 1/51 (1%)
Frame = +2
Query: 167 VSLADLQADTDAERSFRKFRL-IAEYVQGRMCSATSTAWTSQPISSGGWLK 316
++L D + +D + F +F + I + +QGR + T W S+ S GW+K
Sbjct: 224 LTLVDPETISDGTKIFVEFTIRIFDQLQGRHIAGKVTKWFSRSSSEHGWVK 274
>At2g46530.1 68415.m05802 transcriptional factor B3 family protein /
auxin-responsive factor AUX/IAA-related contains Pfam
profiles: PF02309 AUX/IAA family, PF02362 B3 DNA binding
domain
Length = 601
Score = 31.5 bits (68), Expect = 0.56
Identities = 18/49 (36%), Positives = 27/49 (55%), Gaps = 1/49 (2%)
Frame = -1
Query: 333 DESLPFFNHPPELIGCEVHAVEV-AEHIRPCTYSAINLNLRKDLSASVS 190
D+ +P FN PP+++ C V +V + AEH Y+ I L +D S S
Sbjct: 61 DQEIPVFNLPPKIL-CRVLSVTLKAEHETDEVYAQITLQPEEDQSEPTS 108
>At3g52640.1 68416.m05799 nicastrin-related contains weak similarity
to Nicastrin precursor (Swiss-Prot:Q92542) [Homo
sapiens]
Length = 676
Score = 30.3 bits (65), Expect = 1.3
Identities = 19/58 (32%), Positives = 32/58 (55%), Gaps = 3/58 (5%)
Frame = -1
Query: 486 NDFTH-FLSDCSDLSVLGVARLA--LAQGVLIGETNAEDT*NVSIGCLHINVGFDESL 322
N F H L D S+++ V A +A+ + I ++ +DT N ++G +H+N F E L
Sbjct: 430 NKFYHSHLDDLSNINSSSVVAAASVVARTLYILASDNKDTSNSALGSIHVNASFVEEL 487
>At1g74390.1 68414.m08618 exonuclease family protein contains
exonuclease domain, Pfam:PF00929
Length = 506
Score = 28.7 bits (61), Expect = 3.9
Identities = 13/18 (72%), Positives = 15/18 (83%)
Frame = -1
Query: 675 RESSQSTSRSPKTGPSST 622
RESS S+S SPKT PSS+
Sbjct: 220 RESSTSSSSSPKTDPSSS 237
>At5g42470.1 68418.m05170 expressed protein low similarity to BRE
alpha b isoform [Homo sapiens] GI:16326573
Length = 382
Score = 28.3 bits (60), Expect = 5.2
Identities = 14/48 (29%), Positives = 23/48 (47%)
Frame = -1
Query: 318 FFNHPPELIGCEVHAVEVAEHIRPCTYSAINLNLRKDLSASVSACRSA 175
+ H E + ++H A +R A++LNL + L A + CR A
Sbjct: 247 YLPHLEETLERQIHEAVAAIDLRRSFIEALSLNLGRPLEADPTFCRKA 294
>At2g17930.1 68415.m02076 FAT domain-containing protein /
phosphatidylinositol 3- and 4-kinase family protein
contains Pfam profiles PF02259 FAT domain, PF00454
Phosphatidylinositol 3- and 4-kinase, PF02260: FATC
domain
Length = 3795
Score = 27.9 bits (59), Expect = 6.8
Identities = 16/49 (32%), Positives = 27/49 (55%)
Frame = -3
Query: 157 SSLQSFRSNFRSLGTVDKRGADLPLAEHRRSLDIVPIFASEWVDNLLLN 11
S LQS RS F +L + K A + ++ + L++V + S+W+ LN
Sbjct: 1576 SWLQSNRSVFDTLVLIWKSPARISRLQNEQELNLVQVKESKWLVKCFLN 1624
>At4g00890.1 68417.m00120 proline-rich family protein contains
proline-rich extensin domains, INTERPRO:IPR002965
Length = 431
Score = 27.5 bits (58), Expect = 9.0
Identities = 18/62 (29%), Positives = 26/62 (41%)
Frame = -1
Query: 699 PPCLHRLRRESSQSTSRSPKTGPSSTLSLSDAKHRAEGKMPWQALLDVLGNGVRKSTCSP 520
PP L E+ QS S P P++ +SLS K A + + ++T SP
Sbjct: 267 PPLLSPKASENHQSKSPMPPPSPTAQISLSSLKSPIPSPATITAPPPPFSSPLSQTTPSP 326
Query: 519 PP 514
P
Sbjct: 327 KP 328
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,134,813
Number of Sequences: 28952
Number of extensions: 353579
Number of successful extensions: 1077
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1036
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1075
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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