Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= tesS0119
(648 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g67270.1 68418.m08480 microtubule-associated EB1 family prote... 87 7e-18
At5g62500.1 68418.m07844 microtubule-associated EB1 family prote... 85 4e-17
At3g47690.1 68416.m05194 microtubule-associated EB1 family prote... 84 7e-17
At3g19090.1 68416.m02426 RNA-binding protein, putative similar t... 28 4.7
At4g06479.1 68417.m00885 hypothetical protein 28 6.1
At3g52720.2 68416.m05809 carbonic anhydrase family protein low s... 28 6.1
At3g52720.1 68416.m05808 carbonic anhydrase family protein low s... 28 6.1
>At5g67270.1 68418.m08480 microtubule-associated EB1 family protein
similar to SP|Q9UPY8 Microtubule-associated protein
RP/EB family member 3 (Protein EB3) {Homo sapiens};
contains Pfam profiles PF00307: Calponin homology (CH)
domain, PF03271: EB1 protein
Length = 329
Score = 87.4 bits (207), Expect = 7e-18
Identities = 36/71 (50%), Positives = 49/71 (69%)
Frame = +2
Query: 254 HCQFMDMLFPGSVPMKRIKFKTNLEHEYIQNFKILQAGFKKMGVDKIVPIDKLVKGRFQD 433
HCQ MD + PG+VPM ++ F E+E IQN+K+LQ F K+ + K + + KLVKGR D
Sbjct: 43 HCQLMDSVHPGTVPMHKVNFDAKSEYEMIQNYKVLQDVFNKLKITKHIEVSKLVKGRPLD 102
Query: 434 NFEFLQWFKKF 466
N EF+QW KK+
Sbjct: 103 NLEFMQWMKKY 113
Score = 38.7 bits (86), Expect = 0.003
Identities = 14/28 (50%), Positives = 21/28 (75%)
Frame = +3
Query: 168 LSRHDMLAWVNDCLQSNFAKIEELCTGA 251
+ R ++LAW+N LQ N +K+EE C+GA
Sbjct: 14 VGRSEILAWINSTLQLNLSKVEEACSGA 41
>At5g62500.1 68418.m07844 microtubule-associated EB1 family protein
similar to EBF3-S (Microtubule-associated protein) [Homo
sapiens] GI:12751131; contains Pfam profiles PF00307:
Calponin homology (CH) domain, PF03271: EB1 protein
Length = 293
Score = 85.0 bits (201), Expect = 4e-17
Identities = 35/70 (50%), Positives = 50/70 (71%)
Frame = +2
Query: 257 CQFMDMLFPGSVPMKRIKFKTNLEHEYIQNFKILQAGFKKMGVDKIVPIDKLVKGRFQDN 436
CQ +DM FPG VPM ++ F+ E+E IQN+K++Q F K+ + K + +++LVKGR DN
Sbjct: 44 CQMLDMTFPGVVPMHKVNFEAKNEYEMIQNYKVMQEVFTKLKITKPLEVNRLVKGRPLDN 103
Query: 437 FEFLQWFKKF 466
EFLQW K+F
Sbjct: 104 LEFLQWLKRF 113
Score = 33.5 bits (73), Expect = 0.12
Identities = 12/28 (42%), Positives = 21/28 (75%)
Frame = +3
Query: 168 LSRHDMLAWVNDCLQSNFAKIEELCTGA 251
+ R+++L+W+ND L N ++IEE +GA
Sbjct: 14 VGRNEILSWINDRLHLNLSRIEEAASGA 41
>At3g47690.1 68416.m05194 microtubule-associated EB1 family protein
similar to SP|Q9UPY8 Microtubule-associated protein
RP/EB family member 3 (Protein EB3) {Homo sapiens};
contains Pfam profile PF03271: EB1 protein
Length = 276
Score = 84.2 bits (199), Expect = 7e-17
Identities = 36/70 (51%), Positives = 49/70 (70%)
Frame = +2
Query: 257 CQFMDMLFPGSVPMKRIKFKTNLEHEYIQNFKILQAGFKKMGVDKIVPIDKLVKGRFQDN 436
CQ +DM FPG VPM ++ F E++ IQN+K+LQ F K+ + K + I++LVKGR DN
Sbjct: 44 CQMLDMTFPGVVPMHKVNFDAKNEYDMIQNYKVLQDVFNKLKITKPLEINRLVKGRPLDN 103
Query: 437 FEFLQWFKKF 466
EFLQW K+F
Sbjct: 104 LEFLQWLKRF 113
Score = 32.7 bits (71), Expect = 0.22
Identities = 11/28 (39%), Positives = 20/28 (71%)
Frame = +3
Query: 168 LSRHDMLAWVNDCLQSNFAKIEELCTGA 251
+ R+++L W+ND L N +++EE +GA
Sbjct: 14 VGRNEILTWINDRLHLNLSRVEEAASGA 41
>At3g19090.1 68416.m02426 RNA-binding protein, putative similar to
RNA-binding protein homolog GB:AAF00075 GI:6449448 from
[Brassica napus]; contains InterPro entry IPR000504:
RNA-binding region RNP-1 (RNA recognition motif) (RRM)
Length = 455
Score = 28.3 bits (60), Expect = 4.7
Identities = 19/69 (27%), Positives = 32/69 (46%), Gaps = 1/69 (1%)
Frame = -3
Query: 289 TAREQHVHELAMAAPVHNSSIFAKFDWRQSFTHASISCRD-RFSDVTFVEYTFTAIFTMY 113
TA+ QHVH+ A A + N ++ S + ++ D R V VEY FT + +
Sbjct: 104 TAQHQHVHDPAAAFYISNPAVQFPASQNSSSSSKNLLSDDLRLKIVKQVEYQFTDMSLLA 163
Query: 112 QPLMKKFLT 86
+ K ++
Sbjct: 164 NESISKHIS 172
>At4g06479.1 68417.m00885 hypothetical protein
Length = 370
Score = 27.9 bits (59), Expect = 6.1
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = -3
Query: 520 SPSRRPCVICPHLHSWRQEFLEPLQKL 440
+P++RPC IC H +E L P Q +
Sbjct: 292 TPAKRPCEICSHTDHPTEECLYPPQTI 318
>At3g52720.2 68416.m05809 carbonic anhydrase family protein low
similarity to storage protein (dioscorin) [Dioscorea
cayenensis] GI:433463; contains Pfam profile PF00194:
Eukaryotic-type carbonic anhydrase
Length = 230
Score = 27.9 bits (59), Expect = 6.1
Identities = 9/20 (45%), Positives = 13/20 (65%)
Frame = -3
Query: 298 HWYTAREQHVHELAMAAPVH 239
HW+T E H+H + AA +H
Sbjct: 119 HWHTPSEHHLHGVQYAAELH 138
>At3g52720.1 68416.m05808 carbonic anhydrase family protein low
similarity to storage protein (dioscorin) [Dioscorea
cayenensis] GI:433463; contains Pfam profile PF00194:
Eukaryotic-type carbonic anhydrase
Length = 284
Score = 27.9 bits (59), Expect = 6.1
Identities = 9/20 (45%), Positives = 13/20 (65%)
Frame = -3
Query: 298 HWYTAREQHVHELAMAAPVH 239
HW+T E H+H + AA +H
Sbjct: 119 HWHTPSEHHLHGVQYAAELH 138
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,396,583
Number of Sequences: 28952
Number of extensions: 308150
Number of successful extensions: 727
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 705
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 727
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1344285648
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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