Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ps4M0235.Seq
(823 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / hea... 111 4e-25
At5g56010.1 68418.m06989 heat shock protein, putative strong sim... 107 7e-24
At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearl... 107 9e-24
At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearl... 106 2e-23
At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata format... 56 4e-08
At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata format... 56 4e-08
At3g07770.1 68416.m00947 heat shock protein-related strong simil... 43 3e-04
At2g04030.2 68415.m00372 heat shock protein, putative strong sim... 41 0.001
At2g04030.1 68415.m00371 heat shock protein, putative strong sim... 41 0.001
At5g20270.1 68418.m02413 expressed protein contains Pfam domain,... 28 6.5
At3g16480.1 68416.m02103 mitochondrial processing peptidase alph... 28 8.6
>At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / heat
shock protein 83 (HSP83) nearly identical to SP|P27323
Heat shock protein 81-1 (HSP81-1) (Heat shock protein
83) {Arabidopsis thaliana}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00183: Hsp90 protein
Length = 705
Score = 111 bits (268), Expect = 4e-25
Identities = 57/86 (66%), Positives = 69/86 (80%)
Frame = -2
Query: 267 IKAETEADKNDKAVKDLVILLYETALLSSGFTLDEPQVHASRIYRMIKLGLGIDEDEPIQ 88
++ EADKNDK+VKDLV+LLYETALL+SGF+LDEP A+RI+RM+KLGL IDEDE
Sbjct: 624 LRKRAEADKNDKSVKDLVMLLYETALLTSGFSLDEPNTFAARIHRMLKLGLSIDEDE--N 681
Query: 87 VEEPASGDVPPLEGDADDASRMEEVD 10
VEE GD+P LE DA + S+MEEVD
Sbjct: 682 VEE--DGDMPELEEDAAEESKMEEVD 705
>At5g56010.1 68418.m06989 heat shock protein, putative strong
similarity to SP|P55737 Heat shock protein 81-2
(HSP81-2) {Arabidopsis thaliana}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00183: Hsp90 protein
Length = 699
Score = 107 bits (258), Expect = 7e-24
Identities = 52/87 (59%), Positives = 69/87 (79%), Gaps = 1/87 (1%)
Frame = -2
Query: 267 IKAETEADKNDKAVKDLVILLYETALLSSGFTLDEPQVHASRIYRMIKLGLGIDEDEPIQ 88
++ +ADKNDK+VKDLV+LL+ETALL+SGF+LDEP SRI+RM+KLGL ID+D+ ++
Sbjct: 617 LRKRADADKNDKSVKDLVLLLFETALLTSGFSLDEPNTFGSRIHRMLKLGLSIDDDDVVE 676
Query: 87 VEEPASGDVPPLEGDAD-DASRMEEVD 10
A D+PPLE DAD + S+MEEVD
Sbjct: 677 ----ADADMPPLEDDADAEGSKMEEVD 699
>At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearly
identical to heat shock protein hsp81.4 [Arabidopsis
thaliana] GI:1906828; contains Pfam profiles PF02518:
ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like
domain protein, PF00183: Hsp90 protein
Length = 699
Score = 107 bits (257), Expect = 9e-24
Identities = 52/87 (59%), Positives = 69/87 (79%), Gaps = 1/87 (1%)
Frame = -2
Query: 267 IKAETEADKNDKAVKDLVILLYETALLSSGFTLDEPQVHASRIYRMIKLGLGIDEDEPIQ 88
++ EADKNDK+VKDLV+LL+ETALL+SGF+LDEP SRI+RM+KLGL I+ED+ ++
Sbjct: 617 LRKRAEADKNDKSVKDLVLLLFETALLTSGFSLDEPNTFGSRIHRMLKLGLSIEEDDAVE 676
Query: 87 VEEPASGDVPPLEGDAD-DASRMEEVD 10
A ++PPLE DAD + S+MEEVD
Sbjct: 677 ----ADAEMPPLEDDADAEGSKMEEVD 699
>At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearly
identical to SP|P55737 Heat shock protein 81-2 (HSP81-2)
{Arabidopsis thaliana}
Length = 699
Score = 106 bits (255), Expect = 2e-23
Identities = 51/87 (58%), Positives = 69/87 (79%), Gaps = 1/87 (1%)
Frame = -2
Query: 267 IKAETEADKNDKAVKDLVILLYETALLSSGFTLDEPQVHASRIYRMIKLGLGIDEDEPIQ 88
++ +ADKNDK+VKDLV+LL+ETALL+SGF+LDEP SRI+RM+KLGL ID+D+ ++
Sbjct: 617 LRKRADADKNDKSVKDLVLLLFETALLTSGFSLDEPNTFGSRIHRMLKLGLSIDDDDAVE 676
Query: 87 VEEPASGDVPPLEGDAD-DASRMEEVD 10
A ++PPLE DAD + S+MEEVD
Sbjct: 677 ----ADAEMPPLEDDADAEGSKMEEVD 699
>At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata formation
protein, putative nearly identical to SHEPHERD
[Arabidopsis thaliana] GI:19570872; contains Pfam
profiles PF02518: ATPase, histidine kinase-, DNA gyrase
B-, and HSP90-like domain protein, PF00183: Hsp90
protein
Length = 823
Score = 55.6 bits (128), Expect = 4e-08
Identities = 30/80 (37%), Positives = 46/80 (57%), Gaps = 5/80 (6%)
Frame = -2
Query: 267 IKAETEADKNDKAVKDLVILLYETALLSSGFTLDEPQVHASRIYRMIKLGLGID----ED 100
+K +D D++VK+ L+Y+TAL+ SGF L +P+ A+RIY +K GL I D
Sbjct: 720 LKDRIASDPEDESVKETAQLMYQTALIESGFILTDPKDFAARIYNSVKSGLNISPDAVAD 779
Query: 99 EPIQ-VEEPASGDVPPLEGD 43
E I+ EEP + + + D
Sbjct: 780 EEIEAAEEPETSEATETKSD 799
>At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata formation
protein, putative nearly identical to SHEPHERD
[Arabidopsis thaliana] GI:19570872; contains Pfam
profiles PF02518: ATPase, histidine kinase-, DNA gyrase
B-, and HSP90-like domain protein, PF00183: Hsp90
protein
Length = 823
Score = 55.6 bits (128), Expect = 4e-08
Identities = 30/80 (37%), Positives = 46/80 (57%), Gaps = 5/80 (6%)
Frame = -2
Query: 267 IKAETEADKNDKAVKDLVILLYETALLSSGFTLDEPQVHASRIYRMIKLGLGID----ED 100
+K +D D++VK+ L+Y+TAL+ SGF L +P+ A+RIY +K GL I D
Sbjct: 720 LKDRIASDPEDESVKETAQLMYQTALIESGFILTDPKDFAARIYNSVKSGLNISPDAVAD 779
Query: 99 EPIQ-VEEPASGDVPPLEGD 43
E I+ EEP + + + D
Sbjct: 780 EEIEAAEEPETSEATETKSD 799
>At3g07770.1 68416.m00947 heat shock protein-related strong
similarity to heat-shock protein [Secale cereale]
GI:556673; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 803
Score = 42.7 bits (96), Expect = 3e-04
Identities = 26/80 (32%), Positives = 39/80 (48%), Gaps = 4/80 (5%)
Frame = -2
Query: 270 NIKAETEADKNDKAVKDLVILLYETALLSSGFTLDEPQVHASRIYRMIKLGL----GIDE 103
NI A ++ ND+ + L+Y+ AL+SSGFT D P +IY M+ + L E
Sbjct: 713 NINAAYNSNPNDEDAMRAIDLMYDAALVSSGFTPDNPAELGGKIYEMMDVALSGKWSSPE 772
Query: 102 DEPIQVEEPASGDVPPLEGD 43
+P Q + S D E +
Sbjct: 773 VQPQQQQMAHSHDAETFEAE 792
>At2g04030.2 68415.m00372 heat shock protein, putative strong
similarity to heat shock protein [Arabidopsis thaliana]
GI:1906830; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 777
Score = 40.7 bits (91), Expect = 0.001
Identities = 22/55 (40%), Positives = 32/55 (58%)
Frame = -2
Query: 276 CGNIKAETEADKNDKAVKDLVILLYETALLSSGFTLDEPQVHASRIYRMIKLGLG 112
C N TEA + +V LLY+TA++SSGFT D P ++IY M+ + +G
Sbjct: 688 CKNAPESTEATR-------VVDLLYDTAIISSGFTPDSPAELGNKIYEMMAMAVG 735
>At2g04030.1 68415.m00371 heat shock protein, putative strong
similarity to heat shock protein [Arabidopsis thaliana]
GI:1906830; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 780
Score = 40.7 bits (91), Expect = 0.001
Identities = 22/55 (40%), Positives = 32/55 (58%)
Frame = -2
Query: 276 CGNIKAETEADKNDKAVKDLVILLYETALLSSGFTLDEPQVHASRIYRMIKLGLG 112
C N TEA + +V LLY+TA++SSGFT D P ++IY M+ + +G
Sbjct: 691 CKNAPESTEATR-------VVDLLYDTAIISSGFTPDSPAELGNKIYEMMAMAVG 738
>At5g20270.1 68418.m02413 expressed protein contains Pfam domain,
PF03006: Uncharacterised protein family (Hly-III /
UPF0073)
Length = 332
Score = 28.3 bits (60), Expect = 6.5
Identities = 19/54 (35%), Positives = 28/54 (51%)
Frame = -3
Query: 353 VNCNTTHYRANWVPGPPLEINPDHSIVETLRQKRKPTRMTKLLRILLSYCMKLP 192
V+C + ++ VPG + D S R+KRK + T R L+SYC +LP
Sbjct: 13 VSCGNGNCKSKIVPGD--DHGGDESSGTKRRKKRKTQQKTMKRRELMSYC-ELP 63
>At3g16480.1 68416.m02103 mitochondrial processing peptidase alpha
subunit, putative similar to mitochondrial processing
peptidase alpha subunit, mitochondrial precursor,
Alpha-MPP (Ubiquinol-cytochrome C reductase subunit II)
[Potato] SWISS-PROT:P29677
Length = 499
Score = 27.9 bits (59), Expect = 8.6
Identities = 12/37 (32%), Positives = 21/37 (56%)
Frame = -2
Query: 135 RMIKLGLGIDEDEPIQVEEPASGDVPPLEGDADDASR 25
RM+ G+D +E ++V EP D+P + A+ S+
Sbjct: 253 RMVLAASGVDHEELLKVVEPLLSDLPNVPRPAEPKSQ 289
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,132,187
Number of Sequences: 28952
Number of extensions: 382500
Number of successful extensions: 903
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 854
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 899
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1882599200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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