Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0104.Seq
(432 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g20580.1 68415.m02404 26S proteasome regulatory subunit S2 (R... 81 4e-16
At4g28470.1 68417.m04073 26S proteasome regulatory subunit, puta... 79 1e-15
At4g23870.1 68417.m03432 expressed protein predicted proteins, A... 27 4.1
At4g16447.1 68417.m02491 expressed protein 27 5.5
At2g38860.2 68415.m04775 proteaseI (pfpI)-like protein (YLS5) co... 27 7.2
At2g38860.1 68415.m04774 proteaseI (pfpI)-like protein (YLS5) co... 27 7.2
At1g49730.3 68414.m05576 protein kinase family protein contains ... 26 9.6
At1g49730.2 68414.m05577 protein kinase family protein contains ... 26 9.6
At1g49730.1 68414.m05575 protein kinase family protein contains ... 26 9.6
>At2g20580.1 68415.m02404 26S proteasome regulatory subunit S2
(RPN1) contains an APC-complex (cyclosome) and
proteasome component repeat ( PS50248)
Length = 891
Score = 80.6 bits (190), Expect = 4e-16
Identities = 34/59 (57%), Positives = 50/59 (84%)
Frame = +3
Query: 255 GEPAVRRAVPLAIALCSVSNPQLSVIDVLNKYSHDSDNDVAYNAIFAMGLVGAGTNNAR 431
GE +RRAVPLA+ L +SNP+++V+D L++ SHD+D++VA +AI ++GL+GAGTNNAR
Sbjct: 664 GEQNIRRAVPLALGLLCISNPKVTVMDTLSRLSHDTDSEVAMSAIISLGLIGAGTNNAR 722
Score = 30.7 bits (66), Expect = 0.44
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = +3
Query: 324 SVIDVLNKYSHDSDNDVAYNAIFAMGLVGAGTNN 425
S + L+KY H +DN + A+ +G+V G N
Sbjct: 429 SGLSQLDKYFHSNDNPIIAGALLGVGIVNCGIKN 462
>At4g28470.1 68417.m04073 26S proteasome regulatory subunit,
putative contains Pfam domain PF01851:
Proteasome/cyclosome repeat
Length = 1103
Score = 79.4 bits (187), Expect = 1e-15
Identities = 34/59 (57%), Positives = 49/59 (83%)
Frame = +3
Query: 255 GEPAVRRAVPLAIALCSVSNPQLSVIDVLNKYSHDSDNDVAYNAIFAMGLVGAGTNNAR 431
GE +RRAVPLA+ L +SNP+++V+D L++ SHD+D++VA AI ++GL+GAGTNNAR
Sbjct: 736 GEQNIRRAVPLALGLLCISNPKVTVMDTLSRLSHDTDSEVAMAAIISLGLIGAGTNNAR 794
Score = 31.1 bits (67), Expect = 0.34
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +3
Query: 339 LNKYSHDSDNDVAYNAIFAMGLVGAGTNN 425
L+KY H +DN V A+ +G+V G N
Sbjct: 477 LDKYFHSNDNPVVAGALLGVGIVNCGIKN 505
>At4g23870.1 68417.m03432 expressed protein predicted proteins,
Arabidopsis thaliana
Length = 116
Score = 27.5 bits (58), Expect = 4.1
Identities = 8/18 (44%), Positives = 11/18 (61%)
Frame = +2
Query: 155 RCKPWRPLASRSSHWPRK 208
RC+ WRP R + WP +
Sbjct: 96 RCRKWRPSLERITEWPEE 113
>At4g16447.1 68417.m02491 expressed protein
Length = 125
Score = 27.1 bits (57), Expect = 5.5
Identities = 13/30 (43%), Positives = 20/30 (66%)
Frame = +2
Query: 8 LLRNKTRRTQKKLQQLRAVQRTTNPKVNHQ 97
LL+ + R+ Q+ ++LRA + NPK NHQ
Sbjct: 20 LLQERFRQLQRA-RELRAERELLNPKPNHQ 48
>At2g38860.2 68415.m04775 proteaseI (pfpI)-like protein (YLS5)
contains Pfam profile PF01965: DJ-1/PfpI family;
supporting cDNA gi|13122287|dbj|AB047808.1|; identical
to proteaseI (pfpI)-like protein [Arabidopsis thaliana]
GI:13122288, cDNA proteaseI (pfpI)-like protein
GI:13122287
Length = 398
Score = 26.6 bits (56), Expect = 7.2
Identities = 15/46 (32%), Positives = 25/46 (54%), Gaps = 1/46 (2%)
Frame = +3
Query: 297 LCSVSNPQLSVIDVLNKYSHDSDNDVAYNAIFAMGL-VGAGTNNAR 431
+CS+ Q SV+ ++ Y D +V + A+ A+G V A T N +
Sbjct: 202 VCSLEKKQASVLFLIGDYVEDYGINVPFRALQALGCKVDAVTPNKK 247
>At2g38860.1 68415.m04774 proteaseI (pfpI)-like protein (YLS5)
contains Pfam profile PF01965: DJ-1/PfpI family;
supporting cDNA gi|13122287|dbj|AB047808.1|; identical
to proteaseI (pfpI)-like protein [Arabidopsis thaliana]
GI:13122288, cDNA proteaseI (pfpI)-like protein
GI:13122287
Length = 389
Score = 26.6 bits (56), Expect = 7.2
Identities = 15/46 (32%), Positives = 25/46 (54%), Gaps = 1/46 (2%)
Frame = +3
Query: 297 LCSVSNPQLSVIDVLNKYSHDSDNDVAYNAIFAMGL-VGAGTNNAR 431
+CS+ Q SV+ ++ Y D +V + A+ A+G V A T N +
Sbjct: 193 VCSLEKKQASVLFLIGDYVEDYGINVPFRALQALGCKVDAVTPNKK 238
>At1g49730.3 68414.m05576 protein kinase family protein contains
Pfam PF00069: Protein kinase domain; similar to AtPK2324
(GI:1785621) [Arabidopsis thaliana]; similar to
receptor-like protein kinase (GI:1644291) [Catharanthus
roseus]; similar to somatic embryogenesis receptor-like
kinase (GI:2224911) [Daucus carota]
Length = 394
Score = 26.2 bits (55), Expect = 9.6
Identities = 22/93 (23%), Positives = 34/93 (36%), Gaps = 1/93 (1%)
Frame = -2
Query: 431 SCVVSAG-ADESHGEYXXXXXXXXXXXXVFIEHVDDGELRVGHGAQGDGERHGAPHCRLP 255
+C+ S A E +G + +++ DG V Q G H + +CRLP
Sbjct: 28 TCIASISRAMEGYGVSRNATSFCGLGTKILVKYDCDGRTTVTQMHQSPGFGHVSRNCRLP 87
Query: 254 YLPSCPKMRVHISAPVSSANAMTATPRVATACT 156
+ P + S N + A T CT
Sbjct: 88 FSPGHQCRKCLNSGITYLRNLIGAETNNITLCT 120
>At1g49730.2 68414.m05577 protein kinase family protein contains
Pfam PF00069: Protein kinase domain; similar to AtPK2324
(GI:1785621) [Arabidopsis thaliana]; similar to
receptor-like protein kinase (GI:1644291) [Catharanthus
roseus]; similar to somatic embryogenesis receptor-like
kinase (GI:2224911) [Daucus carota]
Length = 450
Score = 26.2 bits (55), Expect = 9.6
Identities = 22/93 (23%), Positives = 34/93 (36%), Gaps = 1/93 (1%)
Frame = -2
Query: 431 SCVVSAG-ADESHGEYXXXXXXXXXXXXVFIEHVDDGELRVGHGAQGDGERHGAPHCRLP 255
+C+ S A E +G + +++ DG V Q G H + +CRLP
Sbjct: 84 TCIASISRAMEGYGVSRNATSFCGLGTKILVKYDCDGRTTVTQMHQSPGFGHVSRNCRLP 143
Query: 254 YLPSCPKMRVHISAPVSSANAMTATPRVATACT 156
+ P + S N + A T CT
Sbjct: 144 FSPGHQCRKCLNSGITYLRNLIGAETNNITLCT 176
>At1g49730.1 68414.m05575 protein kinase family protein contains
Pfam PF00069: Protein kinase domain; similar to AtPK2324
(GI:1785621) [Arabidopsis thaliana]; similar to
receptor-like protein kinase (GI:1644291) [Catharanthus
roseus]; similar to somatic embryogenesis receptor-like
kinase (GI:2224911) [Daucus carota]
Length = 663
Score = 26.2 bits (55), Expect = 9.6
Identities = 22/93 (23%), Positives = 34/93 (36%), Gaps = 1/93 (1%)
Frame = -2
Query: 431 SCVVSAG-ADESHGEYXXXXXXXXXXXXVFIEHVDDGELRVGHGAQGDGERHGAPHCRLP 255
+C+ S A E +G + +++ DG V Q G H + +CRLP
Sbjct: 84 TCIASISRAMEGYGVSRNATSFCGLGTKILVKYDCDGRTTVTQMHQSPGFGHVSRNCRLP 143
Query: 254 YLPSCPKMRVHISAPVSSANAMTATPRVATACT 156
+ P + S N + A T CT
Sbjct: 144 FSPGHQCRKCLNSGITYLRNLIGAETNNITLCT 176
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,144,623
Number of Sequences: 28952
Number of extensions: 90463
Number of successful extensions: 293
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 289
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 293
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 685039728
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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