Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0114.Seq
(648 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 136 2e-32
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 136 2e-32
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 136 2e-32
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 136 2e-32
At1g35550.1 68414.m04414 elongation factor Tu C-terminal domain-... 103 1e-22
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 57 9e-09
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 48 4e-06
At4g27900.2 68417.m04005 expressed protein 32 0.38
At4g27900.1 68417.m04004 expressed protein 32 0.38
At5g50320.1 68418.m06232 radical SAM domain-containing protein /... 29 2.0
At1g55420.1 68414.m06339 DC1 domain-containing protein contains ... 29 3.5
At4g25650.1 68417.m03693 Rieske [2Fe-2S] domain-containing prote... 28 4.7
At1g55380.1 68414.m06334 DC1 domain-containing protein contains ... 28 6.1
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 136 bits (328), Expect = 2e-32
Identities = 69/132 (52%), Positives = 91/132 (68%)
Frame = -2
Query: 647 RWLCAGDSKNNPPKGAADFTAQVIVL*PSWSNLKRLHTSLGLPHCPLACKFAEIKEKVDR 468
R A +SK++P KGAA+FT+QVI++ L +A KF+EI K+DR
Sbjct: 310 RGYVASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDR 369
Query: 467 RTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 288
R+GK E PK +K+GDA +V + P+KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V
Sbjct: 370 RSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSV 429
Query: 287 NFKEAGGGKVTK 252
+ K+ G KVTK
Sbjct: 430 DKKDPTGAKVTK 441
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 136 bits (328), Expect = 2e-32
Identities = 69/132 (52%), Positives = 91/132 (68%)
Frame = -2
Query: 647 RWLCAGDSKNNPPKGAADFTAQVIVL*PSWSNLKRLHTSLGLPHCPLACKFAEIKEKVDR 468
R A +SK++P KGAA+FT+QVI++ L +A KF+EI K+DR
Sbjct: 310 RGYVASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDR 369
Query: 467 RTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 288
R+GK E PK +K+GDA +V + P+KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V
Sbjct: 370 RSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSV 429
Query: 287 NFKEAGGGKVTK 252
+ K+ G KVTK
Sbjct: 430 DKKDPTGAKVTK 441
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 136 bits (328), Expect = 2e-32
Identities = 69/132 (52%), Positives = 91/132 (68%)
Frame = -2
Query: 647 RWLCAGDSKNNPPKGAADFTAQVIVL*PSWSNLKRLHTSLGLPHCPLACKFAEIKEKVDR 468
R A +SK++P KGAA+FT+QVI++ L +A KF+EI K+DR
Sbjct: 310 RGYVASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDR 369
Query: 467 RTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 288
R+GK E PK +K+GDA +V + P+KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V
Sbjct: 370 RSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSV 429
Query: 287 NFKEAGGGKVTK 252
+ K+ G KVTK
Sbjct: 430 DKKDPTGAKVTK 441
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 136 bits (328), Expect = 2e-32
Identities = 69/132 (52%), Positives = 91/132 (68%)
Frame = -2
Query: 647 RWLCAGDSKNNPPKGAADFTAQVIVL*PSWSNLKRLHTSLGLPHCPLACKFAEIKEKVDR 468
R A +SK++P KGAA+FT+QVI++ L +A KF+EI K+DR
Sbjct: 310 RGYVASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDR 369
Query: 467 RTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 288
R+GK E PK +K+GDA +V + P+KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V
Sbjct: 370 RSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSV 429
Query: 287 NFKEAGGGKVTK 252
+ K+ G KVTK
Sbjct: 430 DKKDPTGAKVTK 441
>At1g35550.1 68414.m04414 elongation factor Tu C-terminal
domain-containing protein similar to SP|P13905
Elongation factor 1-alpha (EF-1-alpha) {Arabidopsis
thaliana}; contains Pfam profile PF03143: Elongation
factor Tu C-terminal domain
Length = 104
Score = 103 bits (246), Expect = 1e-22
Identities = 46/83 (55%), Positives = 62/83 (74%)
Frame = -2
Query: 509 LACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVR 330
+A KF+EI K+D RTG E PK +K+ +AAI+N+ P+KP+ VE++ +PPLGRFA+R
Sbjct: 22 IAVKFSEILTKIDWRTGHEIEKEPKFLKNSEAAIINMTPTKPMVVEAYSAYPPLGRFAIR 81
Query: 329 DMRQTVAVGVIKAVNFKEAGGGK 261
DMRQTV VGVIK+V K+ G K
Sbjct: 82 DMRQTVGVGVIKSVVKKDPSGAK 104
Score = 46.0 bits (104), Expect = 2e-05
Identities = 17/21 (80%), Positives = 19/21 (90%)
Frame = -3
Query: 568 NHPGQISNGYTPVLDCHTAHL 506
NH GQI NGYTPVLDCHT+H+
Sbjct: 2 NHLGQIKNGYTPVLDCHTSHI 22
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 57.2 bits (132), Expect = 9e-09
Identities = 23/69 (33%), Positives = 41/69 (59%)
Frame = -2
Query: 503 CKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDM 324
C+ E+K ++D +T K + +K+G A + + + +C+E F +FP LGRF +R
Sbjct: 454 CEIIELKSQIDLKTRKPMKKKVLFVKNGAAVVCRIQVTNSICIEKFSDFPQLGRFTLRTE 513
Query: 323 RQTVAVGVI 297
+T+AVG +
Sbjct: 514 GKTIAVGKV 522
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 48.4 bits (110), Expect = 4e-06
Identities = 22/77 (28%), Positives = 43/77 (55%)
Frame = -2
Query: 518 HCPLACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRF 339
H A ++ +D +TG+ T+ +P+ + + +A++ + P+CVE+F E LGR
Sbjct: 587 HAKEAATVVKLVAMLDPKTGQPTKKSPRCLTAKQSAMLEVSLQNPVCVETFSESRALGRV 646
Query: 338 AVRDMRQTVAVGVIKAV 288
+R +TVA+G + +
Sbjct: 647 FLRSSGRTVAMGKVTRI 663
>At4g27900.2 68417.m04005 expressed protein
Length = 261
Score = 31.9 bits (69), Expect = 0.38
Identities = 11/31 (35%), Positives = 21/31 (67%)
Frame = -2
Query: 440 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 348
PKS+ SG+ + +++V K ++ F +FPP+
Sbjct: 144 PKSVSSGNLSSMDMVEHKDAVIQGFPDFPPV 174
>At4g27900.1 68417.m04004 expressed protein
Length = 261
Score = 31.9 bits (69), Expect = 0.38
Identities = 11/31 (35%), Positives = 21/31 (67%)
Frame = -2
Query: 440 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 348
PKS+ SG+ + +++V K ++ F +FPP+
Sbjct: 144 PKSVSSGNLSSMDMVEHKDAVIQGFPDFPPV 174
>At5g50320.1 68418.m06232 radical SAM domain-containing protein /
GCN5-related N-acetyltransferase (GNAT) family protein
contains Pfam profiles PF00583: acetyltransferase, GNAT
family, PF04055: Radical SAM superfamily
Length = 565
Score = 29.5 bits (63), Expect = 2.0
Identities = 10/38 (26%), Positives = 21/38 (55%)
Frame = -2
Query: 632 GDSKNNPPKGAADFTAQVIVL*PSWSNLKRLHTSLGLP 519
G +N PP+ D A+++ + P W+ + R+ + +P
Sbjct: 353 GRYRNYPPEQLVDIVARILSMVPPWTRVYRVQRDIPMP 390
>At1g55420.1 68414.m06339 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 725
Score = 28.7 bits (61), Expect = 3.5
Identities = 15/38 (39%), Positives = 21/38 (55%), Gaps = 5/38 (13%)
Frame = -3
Query: 118 IFYKACN---VTLFYNLYKVIHNI--SETFCYDCKLKC 20
I+ K C+ V LFY ++ N S FCY C+L+C
Sbjct: 619 IYLKPCHIFKVGLFYKEVEIARNDGNSRLFCYICRLRC 656
>At4g25650.1 68417.m03693 Rieske [2Fe-2S] domain-containing protein
similar to cell death suppressor protein lls1 from Zea
mays [gi:1935909], Rieske iron-sulfur protein Tic55 from
Pisum sativum [gi:2764524]; contains Pfam PF00355 Rieske
[2Fe-2S] domain
Length = 536
Score = 28.3 bits (60), Expect = 4.7
Identities = 11/24 (45%), Positives = 16/24 (66%)
Frame = -2
Query: 500 KFAEIKEKVDRRTGKSTEVNPKSI 429
+F + KEK+DR GK E+N K +
Sbjct: 258 RFPKPKEKIDREGGKPLEINVKKL 281
>At1g55380.1 68414.m06334 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 661
Score = 27.9 bits (59), Expect = 6.1
Identities = 14/38 (36%), Positives = 21/38 (55%), Gaps = 5/38 (13%)
Frame = -3
Query: 118 IFYKACN---VTLFYNLYKVIHNI--SETFCYDCKLKC 20
I+ K C+ V L+Y ++ N S FCY C+L+C
Sbjct: 579 IYLKPCHIFKVGLYYKEVEIARNDGNSRLFCYTCELRC 616
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,827,059
Number of Sequences: 28952
Number of extensions: 276999
Number of successful extensions: 749
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 723
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 741
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1344285648
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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