Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV30118
(666 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g47500.1 68415.m05929 kinesin motor protein-related 50 1e-06
At1g09170.1 68414.m01024 kinesin motor protein-related similar t... 44 1e-04
At5g27000.1 68418.m03221 kinesin motor protein-related non-conse... 43 2e-04
At3g10310.1 68416.m01237 kinesin motor protein-related similar t... 43 2e-04
At1g63640.2 68414.m07198 kinesin motor protein-related C-termina... 42 5e-04
At1g63640.1 68414.m07197 kinesin motor protein-related C-termina... 42 5e-04
At5g48460.1 68418.m05992 fimbrin-like protein, putative strong s... 41 9e-04
At5g55400.1 68418.m06902 fimbrin-like protein, putative similar ... 40 0.001
At5g41310.1 68418.m05020 kinesin motor protein-related 40 0.001
At4g26700.1 68417.m03848 fimbrin-like protein (FIM1) identical t... 38 0.006
At2g04750.1 68415.m00485 fimbrin-like protein, putative similar ... 38 0.008
At5g35700.1 68418.m04269 fimbrin-like protein, putative similar ... 37 0.014
At3g44730.1 68416.m04814 kinesin motor protein-related similar t... 32 0.39
At4g02430.2 68417.m00330 pre-mRNA splicing factor, putative / SR... 29 2.1
At5g37500.1 68418.m04516 guard cell outward rectifying K+ channe... 29 3.7
At1g55970.1 68414.m06419 histone acetyltransferase 4 (HAC4) simi... 28 6.4
At3g52490.1 68416.m05772 heat shock protein-related contains sim... 27 8.5
At2g22740.2 68415.m02696 SET domain-containing protein (SUVH6) i... 27 8.5
At2g22740.1 68415.m02695 SET domain-containing protein (SUVH6) i... 27 8.5
>At2g47500.1 68415.m05929 kinesin motor protein-related
Length = 974
Score = 50.0 bits (114), Expect = 1e-06
Identities = 34/116 (29%), Positives = 59/116 (50%), Gaps = 16/116 (13%)
Frame = +2
Query: 113 ETLEWIRKLTGEPANTSGDADNLYEVLK----DGTLLCKLVNELQPGSVKKINQT----- 265
E W+R++ G A+ E L+ G +LCK++N++QPG+V K+ ++
Sbjct: 49 EAANWLRRMVGVVGAKDLPAEPTEEGLRLGLRSGIILCKVLNKVQPGAVSKVVESPCDAI 108
Query: 266 -------TMAFKCMENINAFLEAVKKLGVPAQETFQTIDLWERQNLYSVVVCLQSL 412
AF+ EN+ FL A++++G P TF+ DL + N VV C+ ++
Sbjct: 109 LVADGAPLSAFQYFENVRNFLVAIQEMGFP---TFEASDLEQGGNASRVVNCVLAI 161
>At1g09170.1 68414.m01024 kinesin motor protein-related similar to
GB:AAB61066
Length = 1010
Score = 43.6 bits (98), Expect = 1e-04
Identities = 34/116 (29%), Positives = 53/116 (45%), Gaps = 16/116 (13%)
Frame = +2
Query: 113 ETLEWIRKLTGEPANTSGDADNLYE----VLKDGTLLCKLVNELQPGSVKK--------- 253
E W+R G AD E L+ G LLC ++N ++PG+V K
Sbjct: 59 EAARWVRNTLGVVGGRDLPADPSEEDFRIALRSGILLCNVLNRVKPGAVPKVVEAPNDPL 118
Query: 254 INQ---TTMAFKCMENINAFLEAVKKLGVPAQETFQTIDLWERQNLYSVVVCLQSL 412
+NQ AF+ EN+ FL V+++G+P TF+ D + +V C+ +L
Sbjct: 119 VNQDGAALSAFQYFENLRNFLVFVEEMGIP---TFEVSDFEKGGKSARIVECVLAL 171
>At5g27000.1 68418.m03221 kinesin motor protein-related
non-consensus AT donor splice site at exon 12;
non-consensus AC acceptor splice site at exon 13
Length = 987
Score = 43.2 bits (97), Expect = 2e-04
Identities = 25/83 (30%), Positives = 45/83 (54%), Gaps = 9/83 (10%)
Frame = +2
Query: 191 LKDGTLLCKLVNELQPGSVKKI---------NQTTMAFKCMENINAFLEAVKKLGVPAQE 343
L+ G +LC ++N++ PGSV K+ AF+ ENI FL A++++G+P
Sbjct: 79 LRSGIVLCNVLNKVNPGSVSKVVEAPDDVADGAALSAFQYFENIRNFLVAIEEMGLP--- 135
Query: 344 TFQTIDLWERQNLYSVVVCLQSL 412
+F+ D+ + +V C+ +L
Sbjct: 136 SFEASDMEKGGKSIRIVNCILAL 158
>At3g10310.1 68416.m01237 kinesin motor protein-related similar to
carboxy-terminal kinesin 2 GB:P79955 [Xenopus laevis]
Length = 897
Score = 42.7 bits (96), Expect = 2e-04
Identities = 34/120 (28%), Positives = 62/120 (51%), Gaps = 16/120 (13%)
Frame = +2
Query: 113 ETLEWIRKLTGEPANTSGDADNLY-EVLKDGTLLCKLVNELQPGSVKKI----------N 259
+ ++W++ + G+ + ++ + L++G +LC +N++ PG+V K+
Sbjct: 24 QAVQWLKSVVGQLGIPNQPSEKEFISCLRNGMILCNAINKIHPGAVSKVVENYSYLNGEY 83
Query: 260 QTTMAFKCMENINAFLEAVKKLGVPAQETFQTIDLWERQNLYS-----VVVCLQSLGRKA 424
Q A++ EN+ FL A++ L +P F+ DL E+ NL S VV C+ LG KA
Sbjct: 84 QLPPAYQYFENVRNFLVALETLRLPG---FEASDL-EKDNLESGSVTKVVDCI--LGLKA 137
>At1g63640.2 68414.m07198 kinesin motor protein-related C-terminal
region is similar to C-term region of kinesin motor
protein GB:AAB51397 (Mus musculus); contains Pfam
profile: PF00225 Kinesin motor domain
Length = 1065
Score = 41.5 bits (93), Expect = 5e-04
Identities = 35/132 (26%), Positives = 62/132 (46%), Gaps = 3/132 (2%)
Frame = +2
Query: 110 QETLEWIRK-LTGEPANTSGDADNLYEVLKDGTLLCKLVNELQPGSVKKINQTTMAFKCM 286
Q +EW+ + L D L L+DGT+LC L+N+L PGS++ A
Sbjct: 43 QSLVEWLNETLPYLKLPWEASEDELRACLRDGTVLCSLLNQLSPGSMRMGGSFEPA---S 99
Query: 287 ENINAFLEAVKKLGVPAQETFQTIDLWERQNLYSVVVCLQSL--GRKAGNFGKPSIGPKE 460
I FL A+ ++ +P F+ D+ E+ ++ V+ L++L G++ K S+ +
Sbjct: 100 VKIERFLTAMDEMALP---RFEVSDI-EQGDMVPVLQSLKALKASFSDGSYDKNSLAARR 155
Query: 461 ADKNVRDFSEEQ 496
D S+ +
Sbjct: 156 RWSLPEDHSDSR 167
>At1g63640.1 68414.m07197 kinesin motor protein-related C-terminal
region is similar to C-term region of kinesin motor
protein GB:AAB51397 (Mus musculus); contains Pfam
profile: PF00225 Kinesin motor domain
Length = 1064
Score = 41.5 bits (93), Expect = 5e-04
Identities = 35/132 (26%), Positives = 62/132 (46%), Gaps = 3/132 (2%)
Frame = +2
Query: 110 QETLEWIRK-LTGEPANTSGDADNLYEVLKDGTLLCKLVNELQPGSVKKINQTTMAFKCM 286
Q +EW+ + L D L L+DGT+LC L+N+L PGS++ A
Sbjct: 43 QSLVEWLNETLPYLKLPWEASEDELRACLRDGTVLCSLLNQLSPGSMRMGGSFEPA---S 99
Query: 287 ENINAFLEAVKKLGVPAQETFQTIDLWERQNLYSVVVCLQSL--GRKAGNFGKPSIGPKE 460
I FL A+ ++ +P F+ D+ E+ ++ V+ L++L G++ K S+ +
Sbjct: 100 VKIERFLTAMDEMALP---RFEVSDI-EQGDMVPVLQSLKALKASFSDGSYDKNSLAARR 155
Query: 461 ADKNVRDFSEEQ 496
D S+ +
Sbjct: 156 RWSLPEDHSDSR 167
>At5g48460.1 68418.m05992 fimbrin-like protein, putative strong
similarity to fimbrin-like protein AtFim2 [Arabidopsis
thaliana] GI:2737926; contains Pfam profile PF00307:
Calponin homology (CH) domain
Length = 654
Score = 40.7 bits (91), Expect = 9e-04
Identities = 25/64 (39%), Positives = 38/64 (59%), Gaps = 4/64 (6%)
Frame = +2
Query: 149 PANTSGDADNLYEVLKDGTLLCKLVNELQPGSV--KKINQTTM--AFKCMENINAFLEAV 316
P N S +++L+EV KDG LLCKL+N PG++ + IN +M ++ EN L +
Sbjct: 149 PINPS--SNDLFEVAKDGVLLCKLINVAVPGTIDERAINTKSMLNPWERNENHTLCLNSA 206
Query: 317 KKLG 328
K +G
Sbjct: 207 KAIG 210
Score = 30.7 bits (66), Expect = 0.91
Identities = 17/55 (30%), Positives = 34/55 (61%), Gaps = 4/55 (7%)
Frame = +2
Query: 173 DNLYEVLKDGTLLCKLVNELQPGSVK-KINQ---TTMAFKCMENINAFLEAVKKL 325
+N++E L+DG +L + ++++ PG V K++ + FK +EN N ++ K+L
Sbjct: 414 NNVFEDLRDGWILLQTLDKVSPGIVNWKVSSKPPIKLPFKKVENCNQVVKLGKQL 468
>At5g55400.1 68418.m06902 fimbrin-like protein, putative similar to
fimbrin-like protein (ATFIM1) [Arabidopsis thaliana]
GI:2905893; contains Pfam profile PF00307: Calponin
homology (CH) domain
Length = 714
Score = 40.3 bits (90), Expect = 0.001
Identities = 15/27 (55%), Positives = 23/27 (85%)
Frame = +2
Query: 167 DADNLYEVLKDGTLLCKLVNELQPGSV 247
D+++LYE++KDG LLCKL+N PG++
Sbjct: 153 DSNDLYELVKDGVLLCKLINIAVPGTI 179
>At5g41310.1 68418.m05020 kinesin motor protein-related
Length = 961
Score = 39.9 bits (89), Expect = 0.001
Identities = 28/90 (31%), Positives = 47/90 (52%), Gaps = 2/90 (2%)
Frame = +2
Query: 95 NDEIAQETLEWIRKLTGEPANTSGDA--DNLYEVLKDGTLLCKLVNELQPGSVKKINQTT 268
N + Q +EW+ + T N +A + L L DGT+LC L+N+L PGS++
Sbjct: 39 NKQGHQSLVEWLNE-TLPYLNLPWEASEEELRACLVDGTVLCNLLNQLSPGSMRMGGSFE 97
Query: 269 MAFKCMENINAFLEAVKKLGVPAQETFQTI 358
C+ NI FL A+ ++ +P E+ + +
Sbjct: 98 PG--CV-NIERFLAAMDEMTLPRFESLKAL 124
>At4g26700.1 68417.m03848 fimbrin-like protein (FIM1) identical to
fimbrin-like protein (ATFIM1) [Arabidopsis thaliana]
GI:2905893
Length = 687
Score = 37.9 bits (84), Expect = 0.006
Identities = 14/26 (53%), Positives = 21/26 (80%)
Frame = +2
Query: 170 ADNLYEVLKDGTLLCKLVNELQPGSV 247
++ LYE++KDG LLCKL+N PG++
Sbjct: 153 SNQLYELVKDGVLLCKLINVAVPGTI 178
>At2g04750.1 68415.m00485 fimbrin-like protein, putative similar to
fimbrin-like protein (ATFIM1) [Arabidopsis thaliana]
GI:2905893; contains Pfam profile PF00307: Calponin
homology (CH) domain
Length = 652
Score = 37.5 bits (83), Expect = 0.008
Identities = 22/64 (34%), Positives = 39/64 (60%), Gaps = 4/64 (6%)
Frame = +2
Query: 149 PANTSGDADNLYEVLKDGTLLCKLVNELQPGSV--KKIN--QTTMAFKCMENINAFLEAV 316
P N + +A L++++KDG LLCKL+N PG++ + IN + ++ EN++ L +
Sbjct: 141 PINPTTNA--LFDLVKDGVLLCKLINIAVPGTIDERAINTKKELNPWERTENLSLCLNSA 198
Query: 317 KKLG 328
K +G
Sbjct: 199 KAIG 202
Score = 34.3 bits (75), Expect = 0.074
Identities = 19/55 (34%), Positives = 36/55 (65%), Gaps = 4/55 (7%)
Frame = +2
Query: 173 DNLYEVLKDGTLLCKLVNELQPGSV--KKINQ--TTMAFKCMENINAFLEAVKKL 325
DN++E +++G +L ++++++ PGSV K N+ M FK +EN N ++ K+L
Sbjct: 408 DNVFEDVRNGWVLLEVLDKVSPGSVNWKHANKPPIKMPFKKVENCNQVIKIGKEL 462
>At5g35700.1 68418.m04269 fimbrin-like protein, putative similar to
fimbrin-like protein (ATFIM1) [Arabidopsis thaliana]
GI:2905893, fimbrin [Schizosaccharomyces pombe]
GI:3057144; contains Pfam profile PF00307: Calponin
homology (CH) domain
Length = 687
Score = 36.7 bits (81), Expect = 0.014
Identities = 19/53 (35%), Positives = 33/53 (62%), Gaps = 4/53 (7%)
Frame = +2
Query: 182 YEVLKDGTLLCKLVNELQPGSV--KKIN--QTTMAFKCMENINAFLEAVKKLG 328
++++KDG LLCKL+N PG++ + IN +T ++ EN+ L + K +G
Sbjct: 156 FDLVKDGVLLCKLINVAVPGTIDERAINTKKTLNPWERNENLTLGLNSAKAIG 208
>At3g44730.1 68416.m04814 kinesin motor protein-related similar to 4
other kinesin-like proteins of A. thaliana: F02P16.12
(PID:g2191180), katA (D11371), katB (D21137), and katC
(D21138); contains non-consensus AT-AC splice sites at
intron 10
Length = 1087
Score = 31.9 bits (69), Expect = 0.39
Identities = 28/93 (30%), Positives = 45/93 (48%), Gaps = 13/93 (13%)
Frame = +2
Query: 173 DNLYEVLKDGTLLCKLVNELQPGSVKKI--NQTT-----------MAFKCMENINAFLEA 313
D L++G +LC ++N++ PGSV K+ N T A + EN+ FL+A
Sbjct: 16 DEFSLALRNGLILCNVLNKVNPGSVLKVVENPITPAIQYADGAAQSAIQYFENMRNFLKA 75
Query: 314 VKKLGVPAQETFQTIDLWERQNLYSVVVCLQSL 412
V+ + + TF DL + + VV C+ L
Sbjct: 76 VEDMQL---LTFGASDLEKGGSSNKVVDCILCL 105
>At4g02430.2 68417.m00330 pre-mRNA splicing factor, putative / SR1
protein, putative strong similarity to SP|O22315
Pre-mRNA splicing factor SF2 (SR1 protein) {Arabidopsis
thaliana}; cDNA NCBI_gi:15810292 supports a truncated
version while protein evidence supports a longer model.
Length = 278
Score = 29.5 bits (63), Expect = 2.1
Identities = 14/33 (42%), Positives = 18/33 (54%)
Frame = -2
Query: 416 YRGTVDKRPPSKGSASPKDQ*SGRSPERARPTS 318
Y D R PS+G + K + GRSP R+R S
Sbjct: 193 YDSRRDSRSPSRGRSYSKSRSRGRSPSRSRSRS 225
>At5g37500.1 68418.m04516 guard cell outward rectifying K+ channel
(GORK) identical to guard cell outward rectifying K+
channel [Arabidopsis thaliana] gi|11414742|emb|CAC17380;
member of the 1 pore, 6 transmembrane (1P/6TM) Shaker K+
channel family, PMID:11500563
Length = 820
Score = 28.7 bits (61), Expect = 3.7
Identities = 14/34 (41%), Positives = 21/34 (61%), Gaps = 2/34 (5%)
Frame = +2
Query: 320 KLGVPAQETFQTIDLWERQN--LYSVVVCLQSLG 415
KLG + E F+ IDLW+R LY +V + ++G
Sbjct: 240 KLGDYSYENFREIDLWKRYTTALYFAIVTMATVG 273
>At1g55970.1 68414.m06419 histone acetyltransferase 4 (HAC4) similar
to CREB-binding protein GB:AAC51770 GI:2443859 from [Homo
sapiens]; contains Pfam PF02135: TAZ zinc finger profile;
contains Pfam PF00569: Zinc finger, ZZ type domain;
identical to histone acetyltransferase HAC4 (GI:14794966)
{Arabidopsis thaliana}
Length = 1456
Score = 27.9 bits (59), Expect = 6.4
Identities = 21/75 (28%), Positives = 35/75 (46%)
Frame = +2
Query: 278 KCMENINAFLEAVKKLGVPAQETFQTIDLWERQNLYSVVVCLQSLGRKAGNFGKPSIGPK 457
K +E FLE ++ P++ +++ ++ Q + +V VCL G FG S GP
Sbjct: 913 KVLEVKERFLELFREENYPSEFPYKSKAIFLFQKIENVEVCL--FGMFVQEFGTDS-GPP 969
Query: 458 EADKNVRDFSEEQLK 502
+ R S E L+
Sbjct: 970 NERRTFRTVSGEALR 984
>At3g52490.1 68416.m05772 heat shock protein-related contains
similarity to 101 kDa heat shock protein; HSP101
[Triticum aestivum] gi|11561808|gb|AAC83689
Length = 815
Score = 27.5 bits (58), Expect = 8.5
Identities = 14/78 (17%), Positives = 35/78 (44%), Gaps = 1/78 (1%)
Frame = +2
Query: 47 KAGINAEAQARIHSKYNDEIAQETLE-WIRKLTGEPANTSGDADNLYEVLKDGTLLCKLV 223
+ + E++AR N + L W+++ E N+ D+D++ E++ +C +
Sbjct: 406 ECSVKFESEARFLKSSNSNVTTVALPAWLQQYKKENQNSHTDSDSIKELVVKWNSICDSI 465
Query: 224 NELQPGSVKKINQTTMAF 277
++ ++ T +F
Sbjct: 466 HKRPSLKTLTLSSPTSSF 483
>At2g22740.2 68415.m02696 SET domain-containing protein (SUVH6)
identical to SUVH6 [Arabidopsis thaliana] GI:13517753;
contains Pfam profiles PF00856: SET domain, PF05033:
Pre-SET motif, PF02182: YDG/SRA domain
Length = 790
Score = 27.5 bits (58), Expect = 8.5
Identities = 19/62 (30%), Positives = 30/62 (48%)
Frame = +2
Query: 392 VVCLQSLGRKAGNFGKPSIGPKEADKNVRDFSEEQLKAGQNVISLQYGTNKGQQSGISFG 571
+ C SL + FG+ S K++ KN+ E L + + + L GT+ G SG S
Sbjct: 212 IACHSSLKVVSREFGEGS-RKKKSKKNLYWRDRESLDSPEQLRILGVGTSSGSSSGDSSR 270
Query: 572 NR 577
N+
Sbjct: 271 NK 272
>At2g22740.1 68415.m02695 SET domain-containing protein (SUVH6)
identical to SUVH6 [Arabidopsis thaliana] GI:13517753;
contains Pfam profiles PF00856: SET domain, PF05033:
Pre-SET motif, PF02182: YDG/SRA domain
Length = 790
Score = 27.5 bits (58), Expect = 8.5
Identities = 19/62 (30%), Positives = 30/62 (48%)
Frame = +2
Query: 392 VVCLQSLGRKAGNFGKPSIGPKEADKNVRDFSEEQLKAGQNVISLQYGTNKGQQSGISFG 571
+ C SL + FG+ S K++ KN+ E L + + + L GT+ G SG S
Sbjct: 212 IACHSSLKVVSREFGEGS-RKKKSKKNLYWRDRESLDSPEQLRILGVGTSSGSSSGDSSR 270
Query: 572 NR 577
N+
Sbjct: 271 NK 272
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,505,969
Number of Sequences: 28952
Number of extensions: 275486
Number of successful extensions: 853
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 829
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 850
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1403159472
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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