Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV30117
(626 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g44350.2 68415.m05517 citrate synthase, mitochondrial, putati... 195 2e-50
At2g44350.1 68415.m05516 citrate synthase, mitochondrial, putati... 194 4e-50
At3g60100.1 68416.m06711 citrate synthase, mitochondrial, putati... 186 8e-48
At2g11270.1 68415.m01208 citrate synthase-related contains simil... 44 8e-05
At3g58740.1 68416.m06547 citrate synthase, glyoxysomal, putative... 41 6e-04
At2g42790.1 68415.m05298 citrate synthase, glyoxysomal, putative... 40 0.001
At3g58750.1 68416.m06548 citrate synthase, glyoxysomal, putative... 38 0.007
At1g59650.1 68414.m06709 expressed protein ; supporting cDNA gi|... 31 0.83
At5g53030.2 68418.m06586 expressed protein 29 2.5
At5g53030.1 68418.m06587 expressed protein 29 2.5
At5g41770.1 68418.m05086 crooked neck protein, putative / cell c... 28 4.4
At4g16442.1 68417.m02489 integral membrane family protein contai... 28 4.4
At4g02260.3 68417.m00305 RelA/SpoT protein, putative (RSH1) iden... 28 4.4
At4g02260.2 68417.m00306 RelA/SpoT protein, putative (RSH1) iden... 28 4.4
At4g02260.1 68417.m00307 RelA/SpoT protein, putative (RSH1) iden... 28 4.4
At3g15750.1 68416.m01995 expressed protein 28 4.4
At5g03280.1 68418.m00277 ethylene-insensitive 2 (EIN2) identical... 28 5.8
At3g52890.2 68416.m05829 protein kinase (KIPK) identical to prot... 28 5.8
At3g52890.1 68416.m05828 protein kinase (KIPK) identical to prot... 28 5.8
>At2g44350.2 68415.m05517 citrate synthase, mitochondrial, putative
strong similarity to SP|P20115 Citrate synthase,
mitochondrial precursor {Arabidopsis thaliana};
contains Pfam profile PF00285: Citrate synthase
Length = 474
Score = 195 bits (476), Expect = 2e-50
Identities = 89/187 (47%), Positives = 133/187 (71%)
Frame = +1
Query: 61 MALFRITSSRLVELQKACPTATVLLRGLSAEQTNLKSILQEKIPKEQEKIREFRKKHGST 240
++ F SR+ Q + + ++ S+ +LKS LQE IP++Q+++++ + +HG
Sbjct: 7 VSAFTRLRSRVQGQQSSLSNSVRWIQMQSSTDLDLKSQLQELIPEQQDRLKKLKSEHGKV 66
Query: 241 KVGEVTVDMMYGGMRGIKGLVWETSVLDADEGIRFRGLSIPECQQQLPKAKGGEEPLPEG 420
++G +TVDM+ GGMRG+ GL+WETS+LD +EGIRFRGLSIPECQ+ LP A+ G EPLPEG
Sbjct: 67 QLGNITVDMVIGGMRGMTGLLWETSLLDPEEGIRFRGLSIPECQKVLPTAQSGAEPLPEG 126
Query: 421 LFWLLVTGDIPTEAQAKALSKEWAARAELPAHVVTMLNNMPGKLHPMSQFSAAVTALNSE 600
L WLL+TG +P++ Q +ALSK+ A RA +P +V ++ +P HPM+QF++ V AL +
Sbjct: 127 LLWLLLTGKVPSKEQVEALSKDLANRAAVPDYVYNAIDALPSTAHPMTQFASGVMALQVQ 186
Query: 601 SKFAKAY 621
S+F KAY
Sbjct: 187 SEFQKAY 193
>At2g44350.1 68415.m05516 citrate synthase, mitochondrial, putative
strong similarity to SP|P20115 Citrate synthase,
mitochondrial precursor {Arabidopsis thaliana};
contains Pfam profile PF00285: Citrate synthase
Length = 473
Score = 194 bits (473), Expect = 4e-50
Identities = 85/159 (53%), Positives = 122/159 (76%)
Frame = +1
Query: 145 SAEQTNLKSILQEKIPKEQEKIREFRKKHGSTKVGEVTVDMMYGGMRGIKGLVWETSVLD 324
S+ +LKS LQE IP++Q+++++ + +HG ++G +TVDM+ GGMRG+ GL+WETS+LD
Sbjct: 34 SSTDLDLKSQLQELIPEQQDRLKKLKSEHGKVQLGNITVDMVIGGMRGMTGLLWETSLLD 93
Query: 325 ADEGIRFRGLSIPECQQQLPKAKGGEEPLPEGLFWLLVTGDIPTEAQAKALSKEWAARAE 504
+EGIRFRGLSIPECQ+ LP A+ G EPLPEGL WLL+TG +P++ Q +ALSK+ A RA
Sbjct: 94 PEEGIRFRGLSIPECQKVLPTAQSGAEPLPEGLLWLLLTGKVPSKEQVEALSKDLANRAA 153
Query: 505 LPAHVVTMLNNMPGKLHPMSQFSAAVTALNSESKFAKAY 621
+P +V ++ +P HPM+QF++ V AL +S+F KAY
Sbjct: 154 VPDYVYNAIDALPSTAHPMTQFASGVMALQVQSEFQKAY 192
>At3g60100.1 68416.m06711 citrate synthase, mitochondrial, putative
strong similarity to SP|Q43175 Citrate synthase,
mitochondrial precursor {Solanum tuberosum}; contains
Pfam profile PF00285: Citrate synthase
Length = 433
Score = 186 bits (454), Expect = 8e-48
Identities = 86/154 (55%), Positives = 117/154 (75%)
Frame = +1
Query: 160 NLKSILQEKIPKEQEKIREFRKKHGSTKVGEVTVDMMYGGMRGIKGLVWETSVLDADEGI 339
+LKS +QE IP++Q+++++ + + G VG +TVDM+ GGMRG+ GL+WETS+LDADEGI
Sbjct: 5 DLKSQMQEIIPEQQDRLKKLKSEQGKVPVGNITVDMVLGGMRGMTGLLWETSLLDADEGI 64
Query: 340 RFRGLSIPECQQQLPKAKGGEEPLPEGLFWLLVTGDIPTEAQAKALSKEWAARAELPAHV 519
RFRG+SIPECQ+ LP A+ GEEPLPE L WLL+TG +PT+ QA ALS E A RA +PA
Sbjct: 65 RFRGMSIPECQKILPSAESGEEPLPESLLWLLLTGKVPTKEQANALSTELAHRAAVPA-- 122
Query: 520 VTMLNNMPGKLHPMSQFSAAVTALNSESKFAKAY 621
++ +P HPM+QF++ V AL +S+F KAY
Sbjct: 123 ---IDALPSTAHPMTQFASGVMALQVQSEFQKAY 153
>At2g11270.1 68415.m01208 citrate synthase-related contains
similarity to Swiss-Prot:P20115 citrate synthase,
mitochondrial precursor [Arabidopsis thaliana]
Length = 83
Score = 44.0 bits (99), Expect = 8e-05
Identities = 17/34 (50%), Positives = 25/34 (73%)
Frame = +1
Query: 199 QEKIREFRKKHGSTKVGEVTVDMMYGGMRGIKGL 300
Q++ ++ + KHG VG +TVDM+ GGMRG+ GL
Sbjct: 43 QDRSKKLKLKHGKVPVGNITVDMVLGGMRGMTGL 76
>At3g58740.1 68416.m06547 citrate synthase, glyoxysomal, putative
strong similarity to SP|P49299 Citrate synthase,
glyoxysomal precursor {Cucurbita maxima}; contains Pfam
profile PF00285: Citrate synthase
Length = 480
Score = 41.1 bits (92), Expect = 6e-04
Identities = 25/95 (26%), Positives = 49/95 (51%), Gaps = 1/95 (1%)
Frame = +1
Query: 313 SVLDADEGI-RFRGLSIPECQQQLPKAKGGEEPLPEGLFWLLVTGDIPTEAQAKALSKEW 489
S +D DEGI R+RG + E ++ + + +LL+ G++P++ Q
Sbjct: 107 SYIDGDEGILRYRGYPVEELAEKSTYTE---------VTYLLIYGNLPSQRQLADWEFAI 157
Query: 490 AARAELPAHVVTMLNNMPGKLHPMSQFSAAVTALN 594
+ + +P V+ M+ +MP +HP+ A++AL+
Sbjct: 158 SQNSAVPQGVLDMIQSMPNDVHPVGALVTAMSALS 192
>At2g42790.1 68415.m05298 citrate synthase, glyoxysomal, putative
strong similarity to SP|P49299 Citrate synthase,
glyoxysomal precursor {Cucurbita maxima}; contains Pfam
profile PF00285: Citrate synthase
Length = 509
Score = 40.3 bits (90), Expect = 0.001
Identities = 27/95 (28%), Positives = 49/95 (51%), Gaps = 1/95 (1%)
Frame = +1
Query: 313 SVLDADEGI-RFRGLSIPECQQQLPKAKGGEEPLPEGLFWLLVTGDIPTEAQAKALSKEW 489
S +D DEGI R+RG I E + + + +LL+ G++P+E+Q
Sbjct: 105 SYIDGDEGILRYRGYPIEEMAENSTFLE---------VAYLLMYGNLPSESQLSDWEFAV 155
Query: 490 AARAELPAHVVTMLNNMPGKLHPMSQFSAAVTALN 594
+ + +P V+ ++ +MP HPM +A++AL+
Sbjct: 156 SQHSAVPQGVLDIIQSMPHDAHPMGVLVSAMSALS 190
>At3g58750.1 68416.m06548 citrate synthase, glyoxysomal, putative
strong similarity to SP|P49299 Citrate synthase,
glyoxysomal precursor {Cucurbita maxima}; contains Pfam
profile PF00285: Citrate synthase
Length = 514
Score = 37.5 bits (83), Expect = 0.007
Identities = 25/93 (26%), Positives = 48/93 (51%), Gaps = 1/93 (1%)
Frame = +1
Query: 319 LDADEGI-RFRGLSIPECQQQLPKAKGGEEPLPEGLFWLLVTGDIPTEAQAKALSKEWAA 495
+D DEGI R+RG I E + + + +LL+ G++P+++Q +
Sbjct: 112 IDGDEGILRYRGYPIEELAESSTFIE---------VAYLLMYGNLPSQSQLADWEFTVSQ 162
Query: 496 RAELPAHVVTMLNNMPGKLHPMSQFSAAVTALN 594
+ +P V+ ++ +MP HPM +A++AL+
Sbjct: 163 HSAVPQGVLDIIQSMPHDAHPMGVLVSAMSALS 195
>At1g59650.1 68414.m06709 expressed protein ; supporting cDNA
gi|6520232|dbj|AB028233.1|
Length = 492
Score = 30.7 bits (66), Expect = 0.83
Identities = 12/29 (41%), Positives = 19/29 (65%)
Frame = +2
Query: 536 ICPANCIPCRSSRLPSPHSTVNLNSLKPT 622
I P+NC+PC +S +PS +L+S P+
Sbjct: 157 ILPSNCLPCLNSTVPSIEKRRSLSSSPPS 185
>At5g53030.2 68418.m06586 expressed protein
Length = 224
Score = 29.1 bits (62), Expect = 2.5
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = +1
Query: 310 TSVLDADEGIRFRGLSIPECQQQLPKAKGGEEPLPE 417
T+VLD +R R LS+P + K +G P PE
Sbjct: 98 TTVLDGPYDLRRRSLSLPRSAAVIRKLRGVPAPAPE 133
>At5g53030.1 68418.m06587 expressed protein
Length = 245
Score = 29.1 bits (62), Expect = 2.5
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = +1
Query: 310 TSVLDADEGIRFRGLSIPECQQQLPKAKGGEEPLPE 417
T+VLD +R R LS+P + K +G P PE
Sbjct: 98 TTVLDGPYDLRRRSLSLPRSAAVIRKLRGVPAPAPE 133
>At5g41770.1 68418.m05086 crooked neck protein, putative / cell
cycle protein, putative similar to Swiss-Prot:P17886
crooked neck protein [Drosophila melanogaster]
Length = 705
Score = 28.3 bits (60), Expect = 4.4
Identities = 13/35 (37%), Positives = 23/35 (65%)
Frame = +1
Query: 169 SILQEKIPKEQEKIREFRKKHGSTKVGEVTVDMMY 273
SI+Q K+PK+ +K + ++ GST+ E +D +Y
Sbjct: 643 SIVQSKLPKKLKKRKAITREDGSTEYEEY-IDYLY 676
>At4g16442.1 68417.m02489 integral membrane family protein contains
TIGRFAM TIGR01569 : plant integral membrane protein
TIGR01569; contains Pfam PF04535 : Domain of unknown
function (DUF588)
Length = 182
Score = 28.3 bits (60), Expect = 4.4
Identities = 12/38 (31%), Positives = 20/38 (52%)
Frame = +2
Query: 257 QLI*CTVACVESKVWFGKPLCWMPMKESVSVVYPSLSA 370
Q + C V ++ KV F KPL W ++ Y +++A
Sbjct: 71 QSVRCVVGTMKGKVLFSKPLAWAFFSGDQAMAYLNVAA 108
>At4g02260.3 68417.m00305 RelA/SpoT protein, putative (RSH1)
identical to RSH1 (RelA/SpoT homolog) [Arabidopsis
thaliana] GI:7141304; contains Pfam profiles PF02824:
TGS domain, PF01966: HD domain, PF04607: Region found in
RelA / SpoT proteins
Length = 816
Score = 28.3 bits (60), Expect = 4.4
Identities = 14/46 (30%), Positives = 23/46 (50%)
Frame = +2
Query: 365 SANSNCPRLRVEKNHYPKVFSGFSSLAIYPPKRKLKRCLKNGRRGR 502
+A S+C L P+ +GF + +PP + C +NGR+ R
Sbjct: 25 NARSDCSALSCAWKA-PRALTGFLASTAHPPVCSVYSCGRNGRKSR 69
>At4g02260.2 68417.m00306 RelA/SpoT protein, putative (RSH1)
identical to RSH1 (RelA/SpoT homolog) [Arabidopsis
thaliana] GI:7141304; contains Pfam profiles PF02824:
TGS domain, PF01966: HD domain, PF04607: Region found in
RelA / SpoT proteins
Length = 883
Score = 28.3 bits (60), Expect = 4.4
Identities = 14/46 (30%), Positives = 23/46 (50%)
Frame = +2
Query: 365 SANSNCPRLRVEKNHYPKVFSGFSSLAIYPPKRKLKRCLKNGRRGR 502
+A S+C L P+ +GF + +PP + C +NGR+ R
Sbjct: 25 NARSDCSALSCAWKA-PRALTGFLASTAHPPVCSVYSCGRNGRKSR 69
>At4g02260.1 68417.m00307 RelA/SpoT protein, putative (RSH1)
identical to RSH1 (RelA/SpoT homolog) [Arabidopsis
thaliana] GI:7141304; contains Pfam profiles PF02824:
TGS domain, PF01966: HD domain, PF04607: Region found in
RelA / SpoT proteins
Length = 884
Score = 28.3 bits (60), Expect = 4.4
Identities = 14/46 (30%), Positives = 23/46 (50%)
Frame = +2
Query: 365 SANSNCPRLRVEKNHYPKVFSGFSSLAIYPPKRKLKRCLKNGRRGR 502
+A S+C L P+ +GF + +PP + C +NGR+ R
Sbjct: 25 NARSDCSALSCAWKA-PRALTGFLASTAHPPVCSVYSCGRNGRKSR 69
>At3g15750.1 68416.m01995 expressed protein
Length = 186
Score = 28.3 bits (60), Expect = 4.4
Identities = 16/46 (34%), Positives = 24/46 (52%)
Frame = +1
Query: 130 LLRGLSAEQTNLKSILQEKIPKEQEKIREFRKKHGSTKVGEVTVDM 267
L+RG+S+ L L+EK+ +QE +F+K H S V M
Sbjct: 79 LVRGVSSALAFLPDELREKLIDKQETREKFQKLHSSVHALSTEVAM 124
>At5g03280.1 68418.m00277 ethylene-insensitive 2 (EIN2) identical to
EIN2 [Arabidopsis thaliana] gi|5231113|gb|AAD41076;
member of the natural resistance-associated macrophage
protein (NRAMP) metal transporter family, PMID:11500563;
metal transport capacity has not been shown,
PMID:11500563, PMID:1038174
Length = 1294
Score = 27.9 bits (59), Expect = 5.8
Identities = 20/68 (29%), Positives = 30/68 (44%), Gaps = 1/68 (1%)
Frame = -2
Query: 229 ASFGIRGSSLVPWESFPGEYFLNSSVQRLDLEVELSQSDMLSAILRVLKM*S*IEPFLKP 50
A G+ G+S+VP + YF S D++ D L AI V S + L
Sbjct: 198 ALMGLLGASIVPHNFYIHSYFAGESTSSSDVDKSSLCQDHLFAIFGVFSGLSLVNYVLMN 257
Query: 49 PSAN-YHT 29
+AN +H+
Sbjct: 258 AAANVFHS 265
>At3g52890.2 68416.m05829 protein kinase (KIPK) identical to protein
kinase KIPK (KCBP-interacting protein kinase)
[Arabidopsis thaliana] gi|7716430|gb|AAF68383
Length = 934
Score = 27.9 bits (59), Expect = 5.8
Identities = 17/42 (40%), Positives = 21/42 (50%), Gaps = 4/42 (9%)
Frame = +1
Query: 346 RGLSIPECQQQLPKAKGGEE----PLPEGLFWLLVTGDIPTE 459
RGL + E + +L KG E P EGL W L+ IP E
Sbjct: 853 RGLLVKEPENRLGSEKGSVEIKRHPFFEGLNWALIRCAIPPE 894
>At3g52890.1 68416.m05828 protein kinase (KIPK) identical to protein
kinase KIPK (KCBP-interacting protein kinase)
[Arabidopsis thaliana] gi|7716430|gb|AAF68383
Length = 934
Score = 27.9 bits (59), Expect = 5.8
Identities = 17/42 (40%), Positives = 21/42 (50%), Gaps = 4/42 (9%)
Frame = +1
Query: 346 RGLSIPECQQQLPKAKGGEE----PLPEGLFWLLVTGDIPTE 459
RGL + E + +L KG E P EGL W L+ IP E
Sbjct: 853 RGLLVKEPENRLGSEKGSVEIKRHPFFEGLNWALIRCAIPPE 894
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.316 0.130 0.375
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,115,944
Number of Sequences: 28952
Number of extensions: 264931
Number of successful extensions: 1014
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 986
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1013
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1275599520
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
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