Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV30038
(529 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g15020.1 68416.m01900 malate dehydrogenase [NAD], mitochondri... 149 1e-36
At1g53240.1 68414.m06033 malate dehydrogenase [NAD], mitochondri... 143 6e-35
At3g47520.1 68416.m05168 malate dehydrogenase [NAD], chloroplast... 143 7e-35
At2g22780.1 68415.m02702 malate dehydrogenase, glyoxysomal, puta... 135 2e-32
At5g09660.1 68418.m01117 malate dehydrogenase, glyoxysomal ident... 122 1e-28
At4g17260.1 68417.m02595 L-lactate dehydrogenase, putative stron... 41 4e-04
At4g21030.1 68417.m03042 Dof-type zinc finger domain-containing ... 29 2.6
At2g47980.1 68415.m06004 expressed protein 27 5.9
At1g51540.1 68414.m05801 kelch repeat-containing protein contain... 27 5.9
>At3g15020.1 68416.m01900 malate dehydrogenase [NAD], mitochondrial,
putative similar to mitochondrial NAD-dependent malate
dehydrogenase GB:CAA10320 SP|Q9ZP06 [Arabidopsis
thaliana]; contains InterPro entry IPR001236:
Lactate/malate dehydrogenase
Length = 341
Score = 149 bits (361), Expect = 1e-36
Identities = 75/151 (49%), Positives = 108/151 (71%), Gaps = 2/151 (1%)
Frame = +3
Query: 48 MFSRALKPAALAVQNGA--KNFSTTSQRNFKXXXXXXXXXIGQPLALLLKQNPLVTRLAL 221
MF + +A V+ G + F++ S + K IGQPL+LL+K NPLV+ L+L
Sbjct: 1 MFRSMIVRSASPVKQGLLRRGFASESVPDRKVVILGAAGGIGQPLSLLMKLNPLVSSLSL 60
Query: 222 YDIAPVTPGVAADLSHVNTPAKVSGHKGPEELSXAIKDADVVVIPXGVPREPGMTRDDLF 401
YDIA TPGVAAD+ H+NT ++VSG+ G ++L A++ AD+V+IP GVPR+PGMTRDDLF
Sbjct: 61 YDIAN-TPGVAADVGHINTRSQVSGYMGDDDLGKALEGADLVIIPAGVPRKPGMTRDDLF 119
Query: 402 NTNASIVRDIALSIAXNAPKAIVAIITNPVN 494
N NA IV++++++IA P+A+V +I+NPVN
Sbjct: 120 NINAGIVKNLSIAIAKYCPQALVNMISNPVN 150
>At1g53240.1 68414.m06033 malate dehydrogenase [NAD], mitochondrial
identical to mitochondrial NAD-dependent malate
dehydrogenase GI:3929649 SP|Q9ZP06 from [Arabidopsis
thaliana]; contains InterPro entry IPR001236:
Lactate/malate dehydrogenase
Length = 341
Score = 143 bits (347), Expect = 6e-35
Identities = 70/132 (53%), Positives = 96/132 (72%)
Frame = +3
Query: 99 KNFSTTSQRNFKXXXXXXXXXIGQPLALLLKQNPLVTRLALYDIAPVTPGVAADLSHVNT 278
++FS+ S K IGQPLALL+K NPLV+ L+LYDIA TPGVAAD+ H+NT
Sbjct: 20 RSFSSGSVPERKVAILGAAGGIGQPLALLMKLNPLVSSLSLYDIAN-TPGVAADVGHINT 78
Query: 279 PAKVSGHKGPEELSXAIKDADVVVIPXGVPREPGMTRDDLFNTNASIVRDIALSIAXNAP 458
++V G+ G + L+ A++ AD+V+IP GVPR+PGMTRDDLFN NA IV+++ +IA P
Sbjct: 79 RSEVVGYMGDDNLAKALEGADLVIIPAGVPRKPGMTRDDLFNINAGIVKNLCTAIAKYCP 138
Query: 459 KAIVAIITNPVN 494
A++ +I+NPVN
Sbjct: 139 HALINMISNPVN 150
>At3g47520.1 68416.m05168 malate dehydrogenase [NAD], chloroplast
(MDH) identical to chloroplast NAD-malate dehydrogenase
[Arabidopsis thaliana] GI:3256066; contains InterPro
entry IPR001236: Lactate/malate dehydrogenase; contains
Pfam profiles PF00056: lactate/malate dehydrogenase, NAD
binding domain and PF02866: lactate/malate
dehydrogenase, alpha/beta C-terminal domain
Length = 403
Score = 143 bits (346), Expect = 7e-35
Identities = 69/111 (62%), Positives = 86/111 (77%)
Frame = +3
Query: 162 IGQPLALLLKQNPLVTRLALYDIAPVTPGVAADLSHVNTPAKVSGHKGPEELSXAIKDAD 341
IGQPL+LL+K +PLV+ L LYDIA V GVAADLSH NTP++V GP EL+ +KD +
Sbjct: 94 IGQPLSLLIKMSPLVSTLHLYDIANVK-GVAADLSHCNTPSQVRDFTGPSELADCLKDVN 152
Query: 342 VVVIPXGVPREPGMTRDDLFNTNASIVRDIALSIAXNAPKAIVAIITNPVN 494
VVVIP GVPR+PGMTRDDLFN NA+IV+ + ++A N P A + II+NPVN
Sbjct: 153 VVVIPAGVPRKPGMTRDDLFNINANIVKTLVEAVAENCPNAFIHIISNPVN 203
>At2g22780.1 68415.m02702 malate dehydrogenase, glyoxysomal,
putative strong similarity to glyoxysomal malate
dehydrogenase (EC 1.1.1.37) SP|P19446 {Citrullus
lanatus}, SP|P46488 {Cucumis sativus}, [Medicago sativa]
GI:2827078, SP|Q42972 {Oryza sativa}, SP|Q9ZP05
{Arabidopsis thaliana}, SP|P37228 {Glycine max};
contains InterPro entry IPR001236: Lactate/malate
dehydrogenase
Length = 354
Score = 135 bits (327), Expect = 2e-32
Identities = 69/122 (56%), Positives = 85/122 (69%)
Frame = +3
Query: 129 FKXXXXXXXXXIGQPLALLLKQNPLVTRLALYDIAPVTPGVAADLSHVNTPAKVSGHKGP 308
FK IGQPLA+L+K NPLV+ L LYD+A PGV AD+SH++T A V G G
Sbjct: 43 FKVAILGAAGGIGQPLAMLMKMNPLVSVLHLYDVANA-PGVTADISHMDTSAVVRGFLGQ 101
Query: 309 EELSXAIKDADVVVIPXGVPREPGMTRDDLFNTNASIVRDIALSIAXNAPKAIVAIITNP 488
+L A+ D+V+IP GVPR+PGMTRDDLFN NA IVR ++ +IA PKAIV II+NP
Sbjct: 102 PQLEEALTGMDLVIIPAGVPRKPGMTRDDLFNINAGIVRTLSEAIAKCCPKAIVNIISNP 161
Query: 489 VN 494
VN
Sbjct: 162 VN 163
>At5g09660.1 68418.m01117 malate dehydrogenase, glyoxysomal
identical to SP|Q9ZP05; identical to cDNA microbody
NAD-dependent malate dehydrogenase GI:3929650
Length = 354
Score = 122 bits (295), Expect = 1e-28
Identities = 61/122 (50%), Positives = 80/122 (65%)
Frame = +3
Query: 129 FKXXXXXXXXXIGQPLALLLKQNPLVTRLALYDIAPVTPGVAADLSHVNTPAKVSGHKGP 308
FK IGQ L+LL+K NPLV+ L LYD+ PGV AD+SH++T A V G G
Sbjct: 43 FKVAILGAAGGIGQSLSLLMKMNPLVSLLHLYDVVNA-PGVTADVSHMDTGAVVRGFLGA 101
Query: 309 EELSXAIKDADVVVIPXGVPREPGMTRDDLFNTNASIVRDIALSIAXNAPKAIVAIITNP 488
++L A+ D+V+IP G+PR+PGMTRDDLF NA IV+ + +A P AIV +I+NP
Sbjct: 102 KQLEDALTGMDLVIIPAGIPRKPGMTRDDLFKINAGIVKTLCEGVAKCCPNAIVNLISNP 161
Query: 489 VN 494
VN
Sbjct: 162 VN 163
>At4g17260.1 68417.m02595 L-lactate dehydrogenase, putative strong
similarity to L-lactate dehydrogenase from Lycopersicon
esculentum (GI:1620970, GI:1620972), Hordeum vulgare
(SP|P22988, SP|P22989); contains InterPro entry
IPR001236: Lactate/malate dehydrogenase
Length = 353
Score = 41.1 bits (92), Expect = 4e-04
Identities = 29/113 (25%), Positives = 52/113 (46%), Gaps = 2/113 (1%)
Frame = +3
Query: 162 IGQPLALLLKQNPLVTRLALYDIAP-VTPGVAADLSHVNTPAKVSGHKGPEELSXAIK-D 335
+G +A + L +AL D P G DL H A + K + +
Sbjct: 51 VGMAIAQTILTQDLADEIALVDAKPDKLRGEMLDLQHA--AAFLPRTKITASVDYEVTAG 108
Query: 336 ADVVVIPXGVPREPGMTRDDLFNTNASIVRDIALSIAXNAPKAIVAIITNPVN 494
+D+ ++ G + PG +R +L N ++ R I +A +P +I+ I++NPV+
Sbjct: 109 SDLCIVTAGARQNPGESRLNLLQRNVALFRHIIPPLAKASPDSILIIVSNPVD 161
>At4g21030.1 68417.m03042 Dof-type zinc finger domain-containing
protein prolamin box binding factor, Zea mays,
PID:g2393775
Length = 194
Score = 28.7 bits (61), Expect = 2.6
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = +2
Query: 8 SNIHVYTR*TNNENVLPRPKTCRPC 82
+N++V+T N NV+P P+ C C
Sbjct: 2 NNLNVFTNEDNEMNVMPPPRVCPRC 26
>At2g47980.1 68415.m06004 expressed protein
Length = 1098
Score = 27.5 bits (58), Expect = 5.9
Identities = 13/33 (39%), Positives = 17/33 (51%)
Frame = +3
Query: 198 PLVTRLALYDIAPVTPGVAADLSHVNTPAKVSG 296
P TRL++ DI + V + HVNT SG
Sbjct: 969 PFATRLSVSDIIDIKKDVQGRIVHVNTDEDPSG 1001
>At1g51540.1 68414.m05801 kelch repeat-containing protein contains
Pfam profile PF01344: Kelch motif
Length = 1036
Score = 27.5 bits (58), Expect = 5.9
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = -3
Query: 395 VITGHSRFTGYSXWDXYNISIFDG 324
++T HS FT WD YN S+ DG
Sbjct: 314 LVTDHSSFTEKEAWDGYNKSL-DG 336
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,478,807
Number of Sequences: 28952
Number of extensions: 243339
Number of successful extensions: 531
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 519
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 526
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 977150592
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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