Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30013
(335 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g24750.1 68417.m03542 expressed protein 30 0.44
At5g35790.1 68418.m04292 glucose-6-phosphate 1-dehydrogenase / G... 29 0.77
At3g06480.1 68416.m00750 DEAD box RNA helicase, putative similar... 29 0.77
At3g03300.1 68416.m00327 DEAD/DEAH box helicase carpel factory-r... 27 2.4
At3g23670.1 68416.m02976 phragmoplast-associated kinesin-related... 27 3.1
At1g44740.1 68414.m05125 expressed protein ; expression supporte... 27 3.1
At3g30380.1 68416.m03835 expressed protein ; expression supporte... 27 4.1
At1g48600.2 68414.m05434 phosphoethanolamine N-methyltransferase... 27 4.1
At1g48600.1 68414.m05435 phosphoethanolamine N-methyltransferase... 27 4.1
At5g13150.1 68418.m01506 exocyst subunit EXO70 family protein le... 26 5.4
At3g48830.1 68416.m05333 polynucleotide adenylyltransferase fami... 26 5.4
At1g63640.2 68414.m07198 kinesin motor protein-related C-termina... 26 5.4
At1g63640.1 68414.m07197 kinesin motor protein-related C-termina... 26 5.4
At5g16560.1 68418.m01938 myb family transcription factor (KAN1) ... 26 7.2
At4g33830.1 68417.m04801 glycosyl hydrolase family 10 protein xy... 26 7.2
At2g13540.1 68415.m01493 mRNA cap-binding protein (ABH1) identic... 26 7.2
At4g33840.1 68417.m04802 glycosyl hydrolase family 10 protein xy... 25 9.5
At1g60900.1 68414.m06856 U2 snRNP auxiliary factor large subunit... 25 9.5
>At4g24750.1 68417.m03542 expressed protein
Length = 260
Score = 29.9 bits (64), Expect = 0.44
Identities = 22/85 (25%), Positives = 39/85 (45%), Gaps = 2/85 (2%)
Frame = -1
Query: 251 LYRAQHPARNGSRLQTIPSP--DIELSYIRTFGAVMHVLRKKTDSIDLRDPNGLRRRVSR 78
L+R QH S + ++ SP LS +R FG + ++ + IDL+ + R
Sbjct: 19 LFRNQHSRMTSSMVSSLKSPIGGTSLSTVRRFGVGVVRMQAVDEDIDLKQMRDIAAAKKR 78
Query: 77 FECETRLVKSHCLEPPDSRGSTVSI 3
++ R K L P ++ G +S+
Sbjct: 79 WDGLLREGKVKLLTPREA-GYAISL 102
>At5g35790.1 68418.m04292 glucose-6-phosphate 1-dehydrogenase / G6PD
(APG1) identical to SP|Q43727 Glucose-6-phosphate
1-dehydrogenase, cytoplasmic isoform (EC 1.1.1.49)
(G6PD) {Arabidopsis thaliana}
Length = 576
Score = 29.1 bits (62), Expect = 0.77
Identities = 11/21 (52%), Positives = 17/21 (80%)
Frame = -1
Query: 293 PGAGLSLNRSQHDLLYRAQHP 231
PG G+ L+RS +LLYR+++P
Sbjct: 477 PGLGMRLDRSDLNLLYRSRYP 497
>At3g06480.1 68416.m00750 DEAD box RNA helicase, putative similar to
RNA helicase DRH1 [Arabidopsis thaliana] GI:3149952;
contains Pfam profiles PF00270: DEAD/DEAH box helicase,
PF00271: Helicase conserved C-terminal domain, PF00397:
WW domain
Length = 1088
Score = 29.1 bits (62), Expect = 0.77
Identities = 23/76 (30%), Positives = 32/76 (42%), Gaps = 3/76 (3%)
Frame = -3
Query: 306 LGRITWRRAESQQIA---ARPALPSTTPRQERKSSTDYSEPRHRTELYPDLRSRDARVKK 136
+GR RR S+ + RP S++ + R S YS R R+ + RSR R +
Sbjct: 944 VGRFDNRRGRSRSRSPDLVRPRRRSSSYSRSRSRSGSYSRSRSRSRSWSRSRSRSPRHSR 1003
Query: 135 KNR*HRSPRSKWASTS 88
H RS S S
Sbjct: 1004 DRGGHNRSRSYSRSPS 1019
>At3g03300.1 68416.m00327 DEAD/DEAH box helicase carpel
factory-related similar to RNA helicase GB:AAF03534
Length = 1317
Score = 27.5 bits (58), Expect = 2.4
Identities = 14/55 (25%), Positives = 26/55 (47%)
Frame = -1
Query: 290 GAGLSLNRSQHDLLYRAQHPARNGSRLQTIPSPDIELSYIRTFGAVMHVLRKKTD 126
G L R+ D Y + R+G +L + P + +I T + +H+ +KK +
Sbjct: 791 GNSLLTKRNSGDQTYIEYYEERHGIQLNFVDEPLLNGRHIFTLHSYLHMAKKKKE 845
>At3g23670.1 68416.m02976 phragmoplast-associated kinesin-related
protein, putative similar to kinesin like protein
GB:CAB10194 from [Arabidopsis thaliana]
Length = 1313
Score = 27.1 bits (57), Expect = 3.1
Identities = 16/47 (34%), Positives = 26/47 (55%)
Frame = -3
Query: 279 ESQQIAARPALPSTTPRQERKSSTDYSEPRHRTELYPDLRSRDARVK 139
ESQ + P+L + +++ KSS + + P L PD RS A++K
Sbjct: 17 ESQ--SPNPSLTKSKSQRKIKSSKENAPPPDLNSLIPDHRSSPAKLK 61
>At1g44740.1 68414.m05125 expressed protein ; expression supported
by MPSS
Length = 311
Score = 27.1 bits (57), Expect = 3.1
Identities = 15/52 (28%), Positives = 27/52 (51%), Gaps = 4/52 (7%)
Frame = +2
Query: 80 ARRDVEAHLDRGD----RCYRFFFLTRASRLRRSGYNSVRCRGSE*SVDDFR 223
A+R+ E L +G+ + + + S++RR YN C G+ ++DD R
Sbjct: 109 AKRNRETDLGQGEVDSFKLHNSIKFSCGSKMRRRRYNVSTCGGASLAIDDVR 160
>At3g30380.1 68416.m03835 expressed protein ; expression supported
by MPSS
Length = 399
Score = 26.6 bits (56), Expect = 4.1
Identities = 14/38 (36%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
Frame = -3
Query: 285 RAESQQIAARPALPSTTPRQERKSSTDYSE-PRHRTEL 175
+A S PA PST R + ++STD E P+ T++
Sbjct: 276 KARSSTDIREPARPSTDQRDKSRTSTDQREMPKLSTDI 313
>At1g48600.2 68414.m05434 phosphoethanolamine N-methyltransferase 2,
putative (NMT2) very similar to |PEM2_ARATH Putative
phosphoethanolamine N-methyltransferase 2 (EC 2.1.1.103)
(SP:Q944H0){Arabidopsis thaliana}; very similar to
Halotolerance protein Hal3b (SP:P94063)[Arabidopsis
thaliana]; to similar to GB:AAF61950 from [Spinacia
oleracea]
Length = 491
Score = 26.6 bits (56), Expect = 4.1
Identities = 12/27 (44%), Positives = 20/27 (74%), Gaps = 1/27 (3%)
Frame = -3
Query: 225 ERKSS-TDYSEPRHRTELYPDLRSRDA 148
+RKS+ T Y EPR T+++ + ++RDA
Sbjct: 168 KRKSNPTHYREPRFYTKVFQECQTRDA 194
>At1g48600.1 68414.m05435 phosphoethanolamine N-methyltransferase 2,
putative (NMT2) very similar to |PEM2_ARATH Putative
phosphoethanolamine N-methyltransferase 2 (EC 2.1.1.103)
(SP:Q944H0){Arabidopsis thaliana}; very similar to
Halotolerance protein Hal3b (SP:P94063)[Arabidopsis
thaliana]; to similar to GB:AAF61950 from [Spinacia
oleracea]
Length = 475
Score = 26.6 bits (56), Expect = 4.1
Identities = 12/27 (44%), Positives = 20/27 (74%), Gaps = 1/27 (3%)
Frame = -3
Query: 225 ERKSS-TDYSEPRHRTELYPDLRSRDA 148
+RKS+ T Y EPR T+++ + ++RDA
Sbjct: 152 KRKSNPTHYREPRFYTKVFQECQTRDA 178
>At5g13150.1 68418.m01506 exocyst subunit EXO70 family protein
leucine zipper-containing protein - Lycopersicon
esculentum, EMBL:Z12127 contains Pfam domain PF03081:
Exo70 exocyst complex subunit;
Length = 653
Score = 26.2 bits (55), Expect = 5.4
Identities = 16/57 (28%), Positives = 28/57 (49%), Gaps = 1/57 (1%)
Frame = -3
Query: 279 ESQQIAARPALPSTTPRQE-RKSSTDYSEPRHRTELYPDLRSRDARVKKKNR*HRSP 112
E+ Q +P P T PRQ+ R+ +Y ++ + DA ++ K+R +R P
Sbjct: 432 EANQTDNKPE-PETKPRQQQREDDEEYKVSAFARQMIRVMELLDANLEIKSRLYRDP 487
>At3g48830.1 68416.m05333 polynucleotide adenylyltransferase family
protein / RNA recognition motif (RRM)-containing protein
similar to SP|P13685 Poly(A) polymerase (EC 2.7.7.19)
{Escherichia coli O157:H7}; contains Pfam profiles
PF01743: polyA polymerase family protein, PF00076: RNA
recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Length = 881
Score = 26.2 bits (55), Expect = 5.4
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = +3
Query: 144 HVHHGSEGPDITQFDVGARNSL*TTSVPGG 233
HVH G++ +++ F A+NSL T G
Sbjct: 142 HVHIGNDMIEVSSFSTSAQNSLRNTRTGSG 171
>At1g63640.2 68414.m07198 kinesin motor protein-related C-terminal
region is similar to C-term region of kinesin motor
protein GB:AAB51397 (Mus musculus); contains Pfam
profile: PF00225 Kinesin motor domain
Length = 1065
Score = 26.2 bits (55), Expect = 5.4
Identities = 15/50 (30%), Positives = 22/50 (44%)
Frame = -1
Query: 158 AVMHVLRKKTDSIDLRDPNGLRRRVSRFECETRLVKSHCLEPPDSRGSTV 9
A MH +R D ++L + + R V R +SHC+ RG V
Sbjct: 636 ASMHCVRSTEDVLELMNIGLMNRTVGATALNERSSRSHCVLSVHVRGVDV 685
>At1g63640.1 68414.m07197 kinesin motor protein-related C-terminal
region is similar to C-term region of kinesin motor
protein GB:AAB51397 (Mus musculus); contains Pfam
profile: PF00225 Kinesin motor domain
Length = 1064
Score = 26.2 bits (55), Expect = 5.4
Identities = 15/50 (30%), Positives = 22/50 (44%)
Frame = -1
Query: 158 AVMHVLRKKTDSIDLRDPNGLRRRVSRFECETRLVKSHCLEPPDSRGSTV 9
A MH +R D ++L + + R V R +SHC+ RG V
Sbjct: 636 ASMHCVRSTEDVLELMNIGLMNRTVGATALNERSSRSHCVLSVHVRGVDV 685
>At5g16560.1 68418.m01938 myb family transcription factor (KAN1)
contains Pfam profile: PF00249 myb-like DNA-binding
domain; identical to cDNA GARP-like putative
transcription factor KANADI1 (KAN1) GI:15723590
Length = 403
Score = 25.8 bits (54), Expect = 7.2
Identities = 15/47 (31%), Positives = 21/47 (44%), Gaps = 1/47 (2%)
Frame = -3
Query: 213 STDYSEPRHRTELYPDL-RSRDARVKKKNR*HRSPRSKWASTSRLAF 76
S+D S P H + + RSR R R+PR +W S+ F
Sbjct: 186 SSDSSSPHHHNHHHHGMIRSRFLPKMPTKRSMRAPRMRWTSSLHARF 232
>At4g33830.1 68417.m04801 glycosyl hydrolase family 10 protein xylan
endohydrolase isoenzyme X-I, Hordeum
vulgare,PID:g1813595
Length = 544
Score = 25.8 bits (54), Expect = 7.2
Identities = 11/20 (55%), Positives = 12/20 (60%)
Frame = +3
Query: 63 RFAFKTRDATSKPIWIAEID 122
R A T AT PIW+ EID
Sbjct: 398 RSALDTLGATGLPIWLTEID 417
>At2g13540.1 68415.m01493 mRNA cap-binding protein (ABH1) identical
to mRNA cap binding protein [Arabidopsis thaliana]
GI:15192738; contains Pfam profile PF02854: MIF4G
domain; identical to cDNA nuclear cap-binding protein
CBP80 GI:8515770
Length = 848
Score = 25.8 bits (54), Expect = 7.2
Identities = 11/24 (45%), Positives = 16/24 (66%)
Frame = -1
Query: 257 DLLYRAQHPARNGSRLQTIPSPDI 186
DLL R Q A NG +L+++P P +
Sbjct: 219 DLLDRIQSLASNGWKLESVPRPHL 242
>At4g33840.1 68417.m04802 glycosyl hydrolase family 10 protein xylan
endohydrolase isoenzyme X-I, Hordeum
vulgare,PID:g1813595
Length = 576
Score = 25.4 bits (53), Expect = 9.5
Identities = 11/20 (55%), Positives = 12/20 (60%)
Frame = +3
Query: 63 RFAFKTRDATSKPIWIAEID 122
R A T AT PIW+ EID
Sbjct: 429 RSALDTFGATGLPIWLTEID 448
>At1g60900.1 68414.m06856 U2 snRNP auxiliary factor large subunit,
putative similar to U2 snRNP auxiliary factor, large
subunit GB:CAA77136 from [Nicotiana plumbaginifolia]
Length = 589
Score = 25.4 bits (53), Expect = 9.5
Identities = 18/50 (36%), Positives = 22/50 (44%), Gaps = 1/50 (2%)
Frame = -3
Query: 231 RQERKSSTDYSEPRHRTELYPDLRSRDARVKKKNR*HRSP-RSKWASTSR 85
R R+S E RHR RSR R + H+S RS+ S SR
Sbjct: 133 RDRRRSRDRDREVRHRRRSRSRSRSRSERRSRSEHRHKSEHRSRSRSRSR 182
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,901,130
Number of Sequences: 28952
Number of extensions: 127299
Number of successful extensions: 386
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 379
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 385
length of database: 12,070,560
effective HSP length: 72
effective length of database: 9,986,016
effective search space used: 389454624
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -