Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30010
(756 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g16640.1 68416.m02127 translationally controlled tumor family... 56 3e-08
At3g05540.1 68416.m00607 translationally controlled tumor family... 55 4e-08
At4g29060.1 68417.m04157 elongation factor Ts family protein sim... 33 0.27
At2g17050.1 68415.m01968 disease resistance protein (TIR-NBS-LRR... 31 0.83
At5g62760.2 68418.m07879 nuclear protein ZAP-related similar to ... 29 2.5
At5g62760.1 68418.m07878 nuclear protein ZAP-related similar to ... 29 2.5
At1g63910.1 68414.m07236 myb family transcription factor (MYB103... 29 3.3
At1g43760.1 68414.m05034 hypothetical protein 29 3.3
At1g80960.3 68414.m09501 F-box protein-related contains weak hit... 28 7.7
At1g80960.2 68414.m09500 F-box protein-related contains weak hit... 28 7.7
At1g80960.1 68414.m09499 F-box protein-related contains weak hit... 28 7.7
At1g08710.1 68414.m00967 F-box family protein similar to ESTs gb... 28 7.7
>At3g16640.1 68416.m02127 translationally controlled tumor family
protein similar to translationally controlled tumor
protein GB:AAD10032 from [Hevea brasiliensis]
Length = 168
Score = 56.0 bits (129), Expect = 3e-08
Identities = 28/66 (42%), Positives = 44/66 (66%), Gaps = 3/66 (4%)
Frame = +2
Query: 62 MKIYKDIITGDEMFSDTYKMKLVDE-VIYEVTGRLVTRAQGDIQIEGFNPSAEEA--DEG 232
M +Y+D++TGDE+ SD++ K ++ +++EV G+ VT D+ I G NPSAEE DEG
Sbjct: 1 MLVYQDLLTGDELLSDSFPYKEIENGILWEVEGKWVTVGAVDVNI-GANPSAEEGGEDEG 59
Query: 233 TDSAVE 250
D + +
Sbjct: 60 VDDSTQ 65
Score = 55.6 bits (128), Expect = 3e-08
Identities = 35/85 (41%), Positives = 44/85 (51%)
Frame = +1
Query: 256 VDIVLNHRLVETYAFGDKKSYTLYLKDYMKKLVAKLEEKAPDQVEVFKTNMNKVMKDILG 435
VDIV RL E + DKK + Y+K Y+K L KL E+ DQ VFK + K +L
Sbjct: 68 VDIVDTFRLQEQPTY-DKKGFIAYIKKYIKLLTPKLSEE--DQA-VFKKGIEGATKFLLP 123
Query: 436 RFKELQFFTGESMDCDGMVAMMEYK 510
R + QFF GE M D + YK
Sbjct: 124 RLSDFQFFVGEGMHDDSTLVFAYYK 148
>At3g05540.1 68416.m00607 translationally controlled tumor family
protein similar to translationally controlled tumor
protein GB:AAD10032 from [Hevea brasiliensis]
Length = 156
Score = 55.2 bits (127), Expect = 4e-08
Identities = 28/74 (37%), Positives = 46/74 (62%)
Frame = +1
Query: 256 VDIVLNHRLVETYAFGDKKSYTLYLKDYMKKLVAKLEEKAPDQVEVFKTNMNKVMKDILG 435
VDI+ RL E +F DKK + +++K Y+K+L KL+ + E+FK ++ K ++
Sbjct: 55 VDIIDTFRLQEQPSF-DKKQFVMFMKRYIKQLSPKLDS---ENQELFKKHIESATKFLMS 110
Query: 436 RFKELQFFTGESMD 477
+ K+ QFF GESM+
Sbjct: 111 KLKDFQFFVGESME 124
Score = 36.7 bits (81), Expect = 0.017
Identities = 18/52 (34%), Positives = 34/52 (65%), Gaps = 1/52 (1%)
Frame = +2
Query: 62 MKIYKDIITGDEMFSDTYKMKLVDE-VIYEVTGRLVTRAQGDIQIEGFNPSA 214
M +Y+DI+TGDE+ SD++ K ++ +++EV G+ + +G + EG + A
Sbjct: 1 MLVYQDILTGDELLSDSFPYKEIENGMLWEVEGKNPSGEEGG-EDEGVDDQA 51
>At4g29060.1 68417.m04157 elongation factor Ts family protein
similar to SP|P35019 Elongation factor Ts (EF-Ts)
{Galdieria sulphuraria}; contains Pfam profiles PF00627:
UBA/TS-N domain, PF00889: Elongation factor TS, PF00575:
S1 RNA binding domain
Length = 953
Score = 32.7 bits (71), Expect = 0.27
Identities = 20/53 (37%), Positives = 31/53 (58%), Gaps = 2/53 (3%)
Frame = +2
Query: 89 GDEMFSDTYKMKLVDEVIYEVT--GRLVTRAQGDIQIEGFNPSAEEADEGTDS 241
G+ S K +++D V+ +T G +T +G+ EGF P+AEEAD+G S
Sbjct: 238 GEGFNSKFAKGQMLDGVVKNLTRSGAFITIGEGE---EGFLPTAEEADDGIGS 287
>At2g17050.1 68415.m01968 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1355
Score = 31.1 bits (67), Expect = 0.83
Identities = 17/49 (34%), Positives = 26/49 (53%), Gaps = 1/49 (2%)
Frame = +1
Query: 334 DYMKKLVAKLEEKAPDQVEVFKTNMNKVMKDILGRFKELQ-FFTGESMD 477
D M+ + KL P +EVFK+N N + ++ F ++ FF GE D
Sbjct: 226 DKMEVKLLKLNHPPPQIMEVFKSNYNALNENEKSMFLDIACFFRGEKAD 274
>At5g62760.2 68418.m07879 nuclear protein ZAP-related similar to
nuclear protein ZAP, Mus musculus, EMBL:AB033168 this
cDNA provides a truncated ORF likely due to a skipped
exon. An alternative ORF is provided.
Length = 383
Score = 29.5 bits (63), Expect = 2.5
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = -3
Query: 520 PSKSYIPSWQPCHHNPWTP 464
P +++ P WQP H N W P
Sbjct: 79 PPQNHYPPWQPHHGNQWRP 97
>At5g62760.1 68418.m07878 nuclear protein ZAP-related similar to
nuclear protein ZAP, Mus musculus, EMBL:AB033168 this
cDNA provides a truncated ORF likely due to a skipped
exon. An alternative ORF is provided.
Length = 661
Score = 29.5 bits (63), Expect = 2.5
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = -3
Query: 520 PSKSYIPSWQPCHHNPWTP 464
P +++ P WQP H N W P
Sbjct: 79 PPQNHYPPWQPHHGNQWRP 97
>At1g63910.1 68414.m07236 myb family transcription factor (MYB103)
contains Pfam profile: PF00249 myb-like DNA-binding
domain
Length = 370
Score = 29.1 bits (62), Expect = 3.3
Identities = 17/52 (32%), Positives = 23/52 (44%)
Frame = -3
Query: 172 SRHQPTGHFVNNFIDQFHFVSVREHLITSDNVLIDLHFDGLEAIKNNKNRKN 17
SRHQP+ V D + E T+ + + +LHFDG N N N
Sbjct: 124 SRHQPSVTTVTLNADTTSIATTIEASTTTTSTIDNLHFDGFTDSPNQLNFTN 175
>At1g43760.1 68414.m05034 hypothetical protein
Length = 626
Score = 29.1 bits (62), Expect = 3.3
Identities = 15/42 (35%), Positives = 22/42 (52%)
Frame = +1
Query: 595 NSIIPNWRLDTFVFLFIYQSCPHPGRNWLLSSQG*TILYFKK 720
+S +P+WRLDT C GR W++ ++L FKK
Sbjct: 181 DSTLPSWRLDT------NYCCSELGRIWIVWDPSVSVLVFKK 216
>At1g80960.3 68414.m09501 F-box protein-related contains weak hit to
Pfam PF00646: F-box domain
Length = 462
Score = 27.9 bits (59), Expect = 7.7
Identities = 16/41 (39%), Positives = 25/41 (60%), Gaps = 3/41 (7%)
Frame = -3
Query: 613 SLELLSYFLSNI*NFS---SSRPCLKNIMIGICVPSKSYIP 500
+L+LLS I F+ +S PCL+ +++GIC KS +P
Sbjct: 213 TLKLLSIVAPEIGVFNRVLASCPCLEVLVLGICCFKKSRVP 253
>At1g80960.2 68414.m09500 F-box protein-related contains weak hit to
Pfam PF00646: F-box domain
Length = 486
Score = 27.9 bits (59), Expect = 7.7
Identities = 16/41 (39%), Positives = 25/41 (60%), Gaps = 3/41 (7%)
Frame = -3
Query: 613 SLELLSYFLSNI*NFS---SSRPCLKNIMIGICVPSKSYIP 500
+L+LLS I F+ +S PCL+ +++GIC KS +P
Sbjct: 213 TLKLLSIVAPEIGVFNRVLASCPCLEVLVLGICCFKKSRVP 253
>At1g80960.1 68414.m09499 F-box protein-related contains weak hit to
Pfam PF00646: F-box domain
Length = 486
Score = 27.9 bits (59), Expect = 7.7
Identities = 16/41 (39%), Positives = 25/41 (60%), Gaps = 3/41 (7%)
Frame = -3
Query: 613 SLELLSYFLSNI*NFS---SSRPCLKNIMIGICVPSKSYIP 500
+L+LLS I F+ +S PCL+ +++GIC KS +P
Sbjct: 213 TLKLLSIVAPEIGVFNRVLASCPCLEVLVLGICCFKKSRVP 253
>At1g08710.1 68414.m00967 F-box family protein similar to ESTs
gb|T22270 and gb|T76886 ; similar to SKP1 interacting
partner 2 (SKIP2) TIGR_Ath1:At5g67250
Length = 274
Score = 27.9 bits (59), Expect = 7.7
Identities = 15/33 (45%), Positives = 18/33 (54%)
Frame = -3
Query: 346 FSYSL*GTMCRISCHRRRMFRLACGSGLCQLAL 248
FSY +C IS RR+FRL+C L L L
Sbjct: 24 FSYK---DLCCISISSRRLFRLSCDDSLWDLLL 53
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,826,403
Number of Sequences: 28952
Number of extensions: 316481
Number of successful extensions: 834
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 805
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 831
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1682736544
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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