Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00046
(836 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g15580.1 68418.m01824 expressed protein unknown protein F14P3... 33 0.18
At2g45840.1 68415.m05701 expressed protein 32 0.41
At3g19020.1 68416.m02415 leucine-rich repeat family protein / ex... 32 0.54
At3g53500.2 68416.m05907 zinc knuckle (CCHC-type) family protein... 31 0.72
At3g53500.1 68416.m05906 zinc knuckle (CCHC-type) family protein... 31 0.72
At4g09290.1 68417.m01537 hypothetical protein 30 2.2
At2g40000.1 68415.m04915 expressed protein 29 2.9
At2g01750.1 68415.m00104 expressed protein 29 2.9
At1g06190.1 68414.m00651 expressed protein 29 2.9
At2g27170.1 68415.m06029 structural maintenance of chromosomes (... 29 3.8
At5g64200.2 68418.m08063 arginine/serine-rich splicing factor SC... 29 5.1
At5g64200.1 68418.m08062 arginine/serine-rich splicing factor SC... 29 5.1
At5g50830.1 68418.m06297 expressed protein 29 5.1
At5g35840.1 68418.m04306 phytochrome C (PHYC) identical to SP|P1... 28 6.7
At3g62900.1 68416.m07066 expressed protein ; expression supporte... 28 6.7
At1g74450.1 68414.m08625 expressed protein 28 6.7
At1g64080.1 68414.m07259 expressed protein identical to hypothet... 28 6.7
At3g12060.1 68416.m01500 expressed protein similar to hypothetic... 28 8.8
At2g48070.2 68415.m06017 expressed protein 28 8.8
At2g48070.1 68415.m06016 expressed protein 28 8.8
At1g75010.1 68414.m08709 MORN (Membrane Occupation and Recogniti... 28 8.8
At1g02840.3 68414.m00246 pre-mRNA splicing factor SF2 (SF2) / SR... 28 8.8
At1g02840.2 68414.m00244 pre-mRNA splicing factor SF2 (SF2) / SR... 28 8.8
At1g02840.1 68414.m00245 pre-mRNA splicing factor SF2 (SF2) / SR... 28 8.8
>At5g15580.1 68418.m01824 expressed protein unknown protein F14P3.18
- Arabidopsis thaliana, EMBL:AC009755
Length = 927
Score = 33.5 bits (73), Expect = 0.18
Identities = 26/84 (30%), Positives = 37/84 (44%), Gaps = 4/84 (4%)
Frame = +2
Query: 515 ESPSRLSTESPVPRSKSPIRHTVETVTTKITKSAAPRGESPKQRPENDKPD----NVPGY 682
ES SRLS S P S S + T ++ + GE+P + P N P +P
Sbjct: 88 ESSSRLSFSSS-PCSSSFSSADISTTASQFEQPGLSNGENPVREPTNGSPRWGGLMMPSD 146
Query: 683 LRPTRTSQIKEETIIHDTEVSSRR 754
+R S I +ET D E S++
Sbjct: 147 IRELVRSSIHKETRTRDEEALSQQ 170
>At2g45840.1 68415.m05701 expressed protein
Length = 523
Score = 32.3 bits (70), Expect = 0.41
Identities = 17/65 (26%), Positives = 32/65 (49%), Gaps = 4/65 (6%)
Frame = +2
Query: 506 RIRESPS-RLSTESPVPRSK--SPIRHTVE-TVTTKITKSAAPRGESPKQRPENDKPDNV 673
RI+ +P +T + +P+ K +P+ T++ ++ I P K +P D+P+
Sbjct: 60 RIKVTPIFTRNTNATIPKEKLTTPLNFTLQCSLDQNIATQTCPASNPEKSQPSKDEPETC 119
Query: 674 PGYLR 688
P Y R
Sbjct: 120 PDYFR 124
>At3g19020.1 68416.m02415 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycopersicon esculentum] gi|5917664|gb|AAD55979;
contains leucine-rich repeats, Pfam:PF00560; contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 956
Score = 31.9 bits (69), Expect = 0.54
Identities = 19/53 (35%), Positives = 29/53 (54%), Gaps = 3/53 (5%)
Frame = +2
Query: 515 ESPSRLSTESPVPRSKSPIRHTVETVTTKITKSAAPRGESPKQ---RPENDKP 664
E+PS + P+ +SP + + +T K K +P+ ESPKQ +PE KP
Sbjct: 451 ETPSHEPSNPKEPKPESPKQESPKTEQPK-PKPESPKQESPKQEAPKPEQPKP 502
Score = 30.3 bits (65), Expect = 1.7
Identities = 20/55 (36%), Positives = 26/55 (47%), Gaps = 3/55 (5%)
Frame = +2
Query: 515 ESPSRLSTE-SPVPRSKSPIRHTV--ETVTTKITKSAAPRGESPKQRPENDKPDN 670
E PS+ E SP P+ SP T E K K +P+ ESPK KP++
Sbjct: 430 EEPSKPKPEESPKPQQPSPKPETPSHEPSNPKEPKPESPKQESPKTEQPKPKPES 484
Score = 30.3 bits (65), Expect = 1.7
Identities = 19/54 (35%), Positives = 29/54 (53%), Gaps = 2/54 (3%)
Frame = +2
Query: 515 ESPSRLS--TESPVPRSKSPIRHTVETVTTKITKSAAPRGESPKQRPENDKPDN 670
ESP + S TE P P+ +SP + + K AP+ E PK +PE+ K ++
Sbjct: 466 ESPKQESPKTEQPKPKPESPKQES--------PKQEAPKPEQPKPKPESPKQES 511
>At3g53500.2 68416.m05907 zinc knuckle (CCHC-type) family protein
contains Pfam domain PF00098: Zinc knuckle
Length = 284
Score = 31.5 bits (68), Expect = 0.72
Identities = 21/63 (33%), Positives = 28/63 (44%), Gaps = 3/63 (4%)
Frame = +2
Query: 488 INQTSRRIRESPSRLSTES---PVPRSKSPIRHTVETVTTKITKSAAPRGESPKQRPEND 658
+ S R R SPSR + S RS+SP+R + A R SPK R ++
Sbjct: 156 VKSRSPRRRRSPSRSRSYSRGRSYSRSRSPVRREKSVEDRSRSPKAMERSVSPKGRDQSL 215
Query: 659 KPD 667
PD
Sbjct: 216 SPD 218
>At3g53500.1 68416.m05906 zinc knuckle (CCHC-type) family protein
contains Pfam domain PF00098: Zinc knuckle
Length = 243
Score = 31.5 bits (68), Expect = 0.72
Identities = 21/63 (33%), Positives = 28/63 (44%), Gaps = 3/63 (4%)
Frame = +2
Query: 488 INQTSRRIRESPSRLSTES---PVPRSKSPIRHTVETVTTKITKSAAPRGESPKQRPEND 658
+ S R R SPSR + S RS+SP+R + A R SPK R ++
Sbjct: 115 VKSRSPRRRRSPSRSRSYSRGRSYSRSRSPVRREKSVEDRSRSPKAMERSVSPKGRDQSL 174
Query: 659 KPD 667
PD
Sbjct: 175 SPD 177
>At4g09290.1 68417.m01537 hypothetical protein
Length = 376
Score = 29.9 bits (64), Expect = 2.2
Identities = 21/83 (25%), Positives = 35/83 (42%), Gaps = 2/83 (2%)
Frame = +2
Query: 500 SRRIRESPS--RLSTESPVPRSKSPIRHTVETVTTKITKSAAPRGESPKQRPENDKPDNV 673
SRR+R + R+S P+ K P + + T K + +SPK+ E K D
Sbjct: 249 SRRLRSMTAARRISKAKTSPKKKEPAKRGRKAATKVTPKVTIKKPKSPKEAKE--KADGD 306
Query: 674 PGYLRPTRTSQIKEETIIHDTEV 742
+ T+ + KE+ D +
Sbjct: 307 TSSVPKTKPEEAKEKADAEDNRL 329
>At2g40000.1 68415.m04915 expressed protein
Length = 435
Score = 29.5 bits (63), Expect = 2.9
Identities = 20/60 (33%), Positives = 29/60 (48%), Gaps = 2/60 (3%)
Frame = -2
Query: 787 SSTKLHAKLHATTTAHFRIMDYSLLLNLTCPRWS*IAWNVIRLVIFRPL--LRRFPSWCS 614
+S KL +KLH T + F+I+ S ++ + P +RL R L R FP W S
Sbjct: 17 NSPKLSSKLHVTIPSPFKIVPVSSPISCSAPALCSAYELYLRLPELRKLWSSRDFPQWTS 76
>At2g01750.1 68415.m00104 expressed protein
Length = 629
Score = 29.5 bits (63), Expect = 2.9
Identities = 20/88 (22%), Positives = 41/88 (46%)
Frame = +2
Query: 551 PRSKSPIRHTVETVTTKITKSAAPRGESPKQRPENDKPDNVPGYLRPTRTSQIKEETIIH 730
PRSK P+ + E + + ++ +G+S K N+ D+VPG L ++
Sbjct: 480 PRSKFPLNQSSEGTSGGGSPNSTKQGDSEKAAGTNN--DSVPGVLHDLLQKEVITLRKAA 537
Query: 731 DTEVSSRRGVEFGVELRRRALNVLQRAV 814
+ + S R + +E+ + + L +A+
Sbjct: 538 NDKDQSLRDKDEAIEMLAKKVETLTKAM 565
>At1g06190.1 68414.m00651 expressed protein
Length = 401
Score = 29.5 bits (63), Expect = 2.9
Identities = 18/67 (26%), Positives = 32/67 (47%)
Frame = +2
Query: 482 NRINQTSRRIRESPSRLSTESPVPRSKSPIRHTVETVTTKITKSAAPRGESPKQRPENDK 661
N+ N S R + S +SPVPRS+SP ++ E + + + + +D+
Sbjct: 218 NKDNNASSFTRPTSS-FRRKSPVPRSQSPPAYSSEATFDQSSSYSVTWTQKKDTVELHDE 276
Query: 662 PDNVPGY 682
P++ P Y
Sbjct: 277 PEHEPAY 283
>At2g27170.1 68415.m06029 structural maintenance of chromosomes
(SMC) family protein similar to basement
membrane-associated chondroitin proteoglycan Bamacan
[Rattus norvegicus] GI:1785540; contains Pfam profile
PF02463: RecF/RecN/SMC N terminal domain. No suitalble
start codon was identified.
Length = 1207
Score = 29.1 bits (62), Expect = 3.8
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +2
Query: 140 EEIRHVTPDRSKETVKLHPKVKDFK 214
E + H+TP+ ++ KL+P++KD K
Sbjct: 777 ELVDHLTPEEREQLSKLNPEIKDLK 801
>At5g64200.2 68418.m08063 arginine/serine-rich splicing factor SC35
contains similarity to splicing factor; contains Pfam
profile PF00076: RNA recognition motif. (a.k.a. RRM,
RBD, or RNP domain)
Length = 303
Score = 28.7 bits (61), Expect = 5.1
Identities = 23/77 (29%), Positives = 30/77 (38%)
Frame = +2
Query: 431 NRGRS*DEHHSN*NRAFNRINQTSRRIRESPSRLSTESPVPRSKSPIRHTVETVTTKITK 610
+R S DE R N SR + SP+R S S P+ SP R V +
Sbjct: 186 SRSASPDEKRRVRGRYDNESRSHSRSLSASPARRSPRSSSPQKTSPARE-VSPDKRSNER 244
Query: 611 SAAPRGESPKQRPENDK 661
S +PR + P K
Sbjct: 245 SPSPRRSLSPRSPALQK 261
>At5g64200.1 68418.m08062 arginine/serine-rich splicing factor SC35
contains similarity to splicing factor; contains Pfam
profile PF00076: RNA recognition motif. (a.k.a. RRM,
RBD, or RNP domain)
Length = 303
Score = 28.7 bits (61), Expect = 5.1
Identities = 23/77 (29%), Positives = 30/77 (38%)
Frame = +2
Query: 431 NRGRS*DEHHSN*NRAFNRINQTSRRIRESPSRLSTESPVPRSKSPIRHTVETVTTKITK 610
+R S DE R N SR + SP+R S S P+ SP R V +
Sbjct: 186 SRSASPDEKRRVRGRYDNESRSHSRSLSASPARRSPRSSSPQKTSPARE-VSPDKRSNER 244
Query: 611 SAAPRGESPKQRPENDK 661
S +PR + P K
Sbjct: 245 SPSPRRSLSPRSPALQK 261
>At5g50830.1 68418.m06297 expressed protein
Length = 281
Score = 28.7 bits (61), Expect = 5.1
Identities = 14/32 (43%), Positives = 19/32 (59%)
Frame = +2
Query: 698 TSQIKEETIIHDTEVSSRRGVEFGVELRRRAL 793
T++IKE+ I E RRG FG+ L R+ L
Sbjct: 222 TTEIKEDGSIVKKEKKERRGKRFGIALPRKYL 253
>At5g35840.1 68418.m04306 phytochrome C (PHYC) identical to
SP|P14714 Phytochrome C {Arabidopsis thaliana}
Length = 1111
Score = 28.3 bits (60), Expect = 6.7
Identities = 18/51 (35%), Positives = 23/51 (45%), Gaps = 4/51 (7%)
Frame = -2
Query: 835 MSTLRSSHCSLQHVQRSSTK----LHAKLHATTTAHFRIMDYSLLLNLTCP 695
MS+ S CS + Q S + AKLH R+ DYS +NL P
Sbjct: 1 MSSNTSRSCSTRSRQNSRVSSQVLVDAKLHGNFEESERLFDYSASINLNMP 51
>At3g62900.1 68416.m07066 expressed protein ; expression supported by
MPSS
Length = 1401
Score = 28.3 bits (60), Expect = 6.7
Identities = 21/81 (25%), Positives = 36/81 (44%)
Frame = +3
Query: 372 VNQKTPKPETLRHKNDHPNVTEDEVEMNTTVIKTERSIESIKPQEEYAKVQVAYPQNHQS 551
V +K K T N N+ D+V T I+ + ++E Q A+ ++
Sbjct: 1117 VRKKVDKCSTSAGNN---NIQADDVTKATAQIRRKSEPSPSPLRKEITSAQAAHNTLKEA 1173
Query: 552 RDLNHRSDILLKRSQRKLQNL 614
+DL H +D LK S L+++
Sbjct: 1174 KDLKHTAD-RLKNSVSNLEHI 1193
>At1g74450.1 68414.m08625 expressed protein
Length = 397
Score = 28.3 bits (60), Expect = 6.7
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = -2
Query: 820 SSHCSLQHVQRSSTKLHAKLHATTTAHFRIMDYSL 716
SS S QH RS + +LH T HFR + +S+
Sbjct: 182 SSSVSSQHRNRSFGRNKEQLHHRTIGHFRSLSWSV 216
>At1g64080.1 68414.m07259 expressed protein identical to
hypothetical protein GB:AAF24565 GI:6692100 from
[Arabidopsis thaliana]
Length = 411
Score = 28.3 bits (60), Expect = 6.7
Identities = 22/85 (25%), Positives = 38/85 (44%), Gaps = 5/85 (5%)
Frame = +2
Query: 569 IRHTVETVTTKITKSAAPRGESPKQRPENDKPD---NVPGYLRPTR--TSQIKEETIIHD 733
+RH+ + +S A + +SP N+KP N+P L+ R + + +
Sbjct: 289 LRHSGQLTAPSSARSTAEKADSPVATKTNNKPGNNINIPAGLKVVRKHLGKSRSSSSTAT 348
Query: 734 TEVSSRRGVEFGVELRRRALNVLQR 808
T S+ V E RRR ++LQ+
Sbjct: 349 TPSSTTATVTTQSESRRRDDSLLQQ 373
>At3g12060.1 68416.m01500 expressed protein similar to hypothetical
protein GB:CAB82953 GI:7340710 from [Arabidopsis
thaliana]
Length = 556
Score = 27.9 bits (59), Expect = 8.8
Identities = 19/61 (31%), Positives = 31/61 (50%)
Frame = +3
Query: 381 KTPKPETLRHKNDHPNVTEDEVEMNTTVIKTERSIESIKPQEEYAKVQVAYPQNHQSRDL 560
+TP PE L N+T + N T ++I +I+ Q +AK ++ P N +RDL
Sbjct: 98 RTPAPENLTAVTK--NLTFESPVANGTTDTNAKTI-TIQFQTGHAKENISCPDNKTARDL 154
Query: 561 N 563
+
Sbjct: 155 D 155
>At2g48070.2 68415.m06017 expressed protein
Length = 197
Score = 27.9 bits (59), Expect = 8.8
Identities = 12/37 (32%), Positives = 19/37 (51%)
Frame = +2
Query: 539 ESPVPRSKSPIRHTVETVTTKITKSAAPRGESPKQRP 649
ESP+P S S + ++ V K + APR + + P
Sbjct: 46 ESPLPSSSSALGKDLKKVVNKTAATFAPRASTASKNP 82
>At2g48070.1 68415.m06016 expressed protein
Length = 197
Score = 27.9 bits (59), Expect = 8.8
Identities = 12/37 (32%), Positives = 19/37 (51%)
Frame = +2
Query: 539 ESPVPRSKSPIRHTVETVTTKITKSAAPRGESPKQRP 649
ESP+P S S + ++ V K + APR + + P
Sbjct: 46 ESPLPSSSSALGKDLKKVVNKTAATFAPRASTASKNP 82
>At1g75010.1 68414.m08709 MORN (Membrane Occupation and Recognition
Nexus) repeat-containing protein
/phosphatidylinositol-4-phosphate 5-kinase-related low
similarity to phosphatidylinositol-4-phosphate 5-kinase
AtPIP5K1 [Arabidopsis thaliana] GI:3702691; contains
Pfam profile PF02493: MORN repeat
Length = 712
Score = 27.9 bits (59), Expect = 8.8
Identities = 15/41 (36%), Positives = 18/41 (43%)
Frame = -3
Query: 654 FSGRCFGDSPLGAADFVIFVVTVSTVCLIGDLDLGTGDSVD 532
F GRC G P G DF++ + G L LG G D
Sbjct: 562 FKGRCQGGLPEGKTDFLVNPKVTADYSHQGRLVLGDGSIYD 602
>At1g02840.3 68414.m00246 pre-mRNA splicing factor SF2 (SF2) / SR1
protein identical to SP|O22315 Pre-mRNA splicing factor
SF2 (SR1 protein) {Arabidopsis thaliana}
Length = 303
Score = 27.9 bits (59), Expect = 8.8
Identities = 17/45 (37%), Positives = 28/45 (62%)
Frame = +2
Query: 431 NRGRS*DEHHSN*NRAFNRINQTSRRIRESPSRLSTESPVPRSKS 565
+RGRS S +R+ +R + ++ R SP++ ++ SP PRSKS
Sbjct: 217 SRGRSVSRSRSR-SRSRSR-SPKAKSSRRSPAKSTSRSPGPRSKS 259
>At1g02840.2 68414.m00244 pre-mRNA splicing factor SF2 (SF2) / SR1
protein identical to SP|O22315 Pre-mRNA splicing factor
SF2 (SR1 protein) {Arabidopsis thaliana}
Length = 285
Score = 27.9 bits (59), Expect = 8.8
Identities = 17/45 (37%), Positives = 28/45 (62%)
Frame = +2
Query: 431 NRGRS*DEHHSN*NRAFNRINQTSRRIRESPSRLSTESPVPRSKS 565
+RGRS S +R+ +R + ++ R SP++ ++ SP PRSKS
Sbjct: 217 SRGRSVSRSRSR-SRSRSR-SPKAKSSRRSPAKSTSRSPGPRSKS 259
>At1g02840.1 68414.m00245 pre-mRNA splicing factor SF2 (SF2) / SR1
protein identical to SP|O22315 Pre-mRNA splicing factor
SF2 (SR1 protein) {Arabidopsis thaliana}
Length = 303
Score = 27.9 bits (59), Expect = 8.8
Identities = 17/45 (37%), Positives = 28/45 (62%)
Frame = +2
Query: 431 NRGRS*DEHHSN*NRAFNRINQTSRRIRESPSRLSTESPVPRSKS 565
+RGRS S +R+ +R + ++ R SP++ ++ SP PRSKS
Sbjct: 217 SRGRSVSRSRSR-SRSRSR-SPKAKSSRRSPAKSTSRSPGPRSKS 259
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,657,579
Number of Sequences: 28952
Number of extensions: 278719
Number of successful extensions: 1017
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 979
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1015
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1931371200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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