Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P01_F_D08
(617 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g17440.2 68414.m02133 transcription initiation factor IID (TF... 86 2e-17
At1g17440.1 68414.m02132 transcription initiation factor IID (TF... 86 2e-17
At3g10070.1 68416.m01207 transcription initiation factor IID (TF... 79 2e-15
At3g50230.1 68416.m05493 leucine-rich repeat transmembrane prote... 29 1.9
At5g02880.1 68418.m00231 HECT-domain-containing protein / ubiqui... 29 2.5
At5g51540.1 68418.m06391 peptidase M3 family protein / thimet ol... 29 3.3
At1g15240.1 68414.m01824 phox (PX) domain-containing protein wea... 28 4.3
At2g16470.1 68415.m01887 zinc finger (CCCH-type) family protein ... 28 5.7
At2g44570.1 68415.m05547 glycosyl hydrolase family 9 protein 27 10.0
At1g21080.1 68414.m02637 DNAJ heat shock N-terminal domain-conta... 27 10.0
>At1g17440.2 68414.m02133 transcription initiation factor IID
(TFIID) subunit A family protein similar to SP|Q16514
Transcription initiation factor TFIID 20/15 kDa subunits
(TAFII-20/TAFII-15) {Homo sapiens}; contains Pfam
profile PF03847: Transcription initiation factor TFIID
subunit A
Length = 683
Score = 86.2 bits (204), Expect = 2e-17
Identities = 43/119 (36%), Positives = 74/119 (62%)
Frame = +3
Query: 249 QNTSIQGSPSQHSPMGTQSQVAKVGQGGAGDQSSQLLSRPRLQELXXEVDPTVQLDEXVE 428
++ S+ GS + + GT + QG + ++QLL + ++Q+L +VD +LD VE
Sbjct: 494 KSVSLTGSQPEATQSGTTTPGGSSSQGT--EATNQLLGKRKIQDLVSQVDVHAKLDPDVE 551
Query: 429 EMLLQLADDFIDTTLNSACALAKHRHAPNVQLXDVQLHLXRQWNMWIPGFGNXELRPYK 605
++LL++ADDFID+ + AC+LAKHR + ++ D+ LHL + ++ IPGF + + R K
Sbjct: 552 DLLLEVADDFIDSVTSFACSLAKHRKSSVLEPKDILLHLEKNLHLTIPGFSSEDKRQTK 610
>At1g17440.1 68414.m02132 transcription initiation factor IID
(TFIID) subunit A family protein similar to SP|Q16514
Transcription initiation factor TFIID 20/15 kDa subunits
(TAFII-20/TAFII-15) {Homo sapiens}; contains Pfam
profile PF03847: Transcription initiation factor TFIID
subunit A
Length = 683
Score = 86.2 bits (204), Expect = 2e-17
Identities = 43/119 (36%), Positives = 74/119 (62%)
Frame = +3
Query: 249 QNTSIQGSPSQHSPMGTQSQVAKVGQGGAGDQSSQLLSRPRLQELXXEVDPTVQLDEXVE 428
++ S+ GS + + GT + QG + ++QLL + ++Q+L +VD +LD VE
Sbjct: 494 KSVSLTGSQPEATQSGTTTPGGSSSQGT--EATNQLLGKRKIQDLVSQVDVHAKLDPDVE 551
Query: 429 EMLLQLADDFIDTTLNSACALAKHRHAPNVQLXDVQLHLXRQWNMWIPGFGNXELRPYK 605
++LL++ADDFID+ + AC+LAKHR + ++ D+ LHL + ++ IPGF + + R K
Sbjct: 552 DLLLEVADDFIDSVTSFACSLAKHRKSSVLEPKDILLHLEKNLHLTIPGFSSEDKRQTK 610
>At3g10070.1 68416.m01207 transcription initiation factor IID
(TFIID) subunit A family protein similar to hypothetical
protein GB:CAB10099 [Schizosaccharomyces pombe];
contains Pfam profile PF03847: Transcription initiation
factor TFIID subunit A
Length = 539
Score = 79.4 bits (187), Expect = 2e-15
Identities = 39/138 (28%), Positives = 72/138 (52%), Gaps = 2/138 (1%)
Frame = +3
Query: 201 QGAIQYVNNPMQSPQLQNTSIQGS--PSQHSPMGTQSQVAKVGQGGAGDQSSQLLSRPRL 374
Q + + + P + L N + PSQ P+ AK ++L + +
Sbjct: 347 QQPLAHPHQPTRVQGLVNQKVTSPVMPSQ-PPVAQPGNHAKTVSAETEPSDDRILGKRSI 405
Query: 375 QELXXEVDPTVQLDEXVEEMLLQLADDFIDTTLNSACALAKHRHAPNVQLXDVQLHLXRQ 554
EL ++DP+ +LD VE++L +A+DF+++ C+LAKHR + ++ D+ LH+ R
Sbjct: 406 HELLQQIDPSEKLDPEVEDILSDIAEDFVESITTFGCSLAKHRKSDILEAKDILLHVERN 465
Query: 555 WNMWIPGFGNXELRPYKR 608
WN+ PGF + E + +++
Sbjct: 466 WNIRPPGFSSDEFKTFRK 483
>At3g50230.1 68416.m05493 leucine-rich repeat transmembrane protein
kinase, putative receptor-like protein kinase (RKL1),
Arabidopsis thaliana, EMBL:AF084034
Length = 660
Score = 29.5 bits (63), Expect = 1.9
Identities = 18/46 (39%), Positives = 23/46 (50%)
Frame = +3
Query: 246 LQNTSIQGSPSQHSPMGTQSQVAKVGQGGAGDQSSQLLSRPRLQEL 383
L+N SI GS SP+ + G +G SS +LS RL EL
Sbjct: 106 LENNSISGSIPDLSPLVNLKTLTLSKNGFSGTLSSSILSLRRLTEL 151
>At5g02880.1 68418.m00231 HECT-domain-containing protein /
ubiquitin-transferase family protein /
armadillo/beta-catenin-like repeat-containing protein
similar to SP|Q14669 Thyroid receptor interacting protein
12 (TRIP12) {Homo sapiens}; contains Pfam profiles
PF00632: HECT-domain (ubiquitin-transferase), PF00514:
Armadillo/beta-catenin-like repeat
Length = 1502
Score = 29.1 bits (62), Expect = 2.5
Identities = 25/92 (27%), Positives = 43/92 (46%), Gaps = 2/92 (2%)
Frame = +3
Query: 234 QSPQLQNTSIQGSPSQHSPMGTQSQVAKVGQGGAGDQSSQLLSRPRL--QELXXEVDPTV 407
QS QLQ+TSI SPM S+ + Q Q SQ+ + +L Q+ + +
Sbjct: 822 QSSQLQSTSISCQAESSSPMEIDSESSDASQ----LQGSQVEDQTQLPGQQNASSSETSS 877
Query: 408 QLDEXVEEMLLQLADDFIDTTLNSACALAKHR 503
+ ++ V +L +L +D +L A+ H+
Sbjct: 878 EKEDAVPRLLFRLEGLELDRSLTVYQAILLHK 909
>At5g51540.1 68418.m06391 peptidase M3 family protein / thimet
oligopeptidase family protein low similarity to
SP|Q99797 Mitochondrial intermediate peptidase,
mitochondrial precursor (EC 3.4.24.59) {Homo sapiens};
contains Pfam profile PF01432: Peptidase family M3
Length = 860
Score = 28.7 bits (61), Expect = 3.3
Identities = 16/64 (25%), Positives = 25/64 (39%)
Frame = +3
Query: 159 LAQAANMPTIGTVGQGAIQYVNNPMQSPQLQNTSIQGSPSQHSPMGTQSQVAKVGQGGAG 338
L A I G+G + NP + + QN+ I+ ++ S + VG G
Sbjct: 677 LTDLAGKEIISVHGEGIVPATTNPRFAIKCQNSQIESDTTEDPSRSKNSSSSGVGFGSPA 736
Query: 339 DQSS 350
SS
Sbjct: 737 SSSS 740
>At1g15240.1 68414.m01824 phox (PX) domain-containing protein weak
similarity to SP|Q9Y5W8 Sorting nexin 13 {Homo sapiens};
contains Pfam profiles PF00787: PX domain, PF02194: PXA
domain
Length = 706
Score = 28.3 bits (60), Expect = 4.3
Identities = 16/37 (43%), Positives = 22/37 (59%)
Frame = +2
Query: 20 DDLIRKINKLVLTHSSRRFRNV*TIIVFIRDVFSLCC 130
++L+ IN VL SRRFRNV I + RD+ + C
Sbjct: 141 EELVFIIND-VLGELSRRFRNVNLIDLLTRDLIDIIC 176
>At2g16470.1 68415.m01887 zinc finger (CCCH-type) family protein /
GYF domain-containing protein contains Pfam domains
PF00642: Zinc finger C-x8-C-x5-C-x3-H type (and
similar), PF02213: GYF domain
Length = 659
Score = 27.9 bits (59), Expect = 5.7
Identities = 22/73 (30%), Positives = 33/73 (45%), Gaps = 4/73 (5%)
Frame = +3
Query: 147 SNNSLAQAANMPTIGTVGQGAIQYVNNPMQSPQLQNTSIQGSPSQHSPM---GTQSQVA- 314
S S ++P + GA+Q P+ +P N S+ S + HSP G QS +
Sbjct: 350 SGQSQTSRIDIPVVVN-SAGALQPQTYPIPTPDPINVSVNHSATLHSPTPAGGKQSWGSM 408
Query: 315 KVGQGGAGDQSSQ 353
+ GG+ SSQ
Sbjct: 409 QTDHGGSNTPSSQ 421
>At2g44570.1 68415.m05547 glycosyl hydrolase family 9 protein
Length = 492
Score = 27.1 bits (57), Expect = 10.0
Identities = 14/49 (28%), Positives = 21/49 (42%)
Frame = +3
Query: 156 SLAQAANMPTIGTVGQGAIQYVNNPMQSPQLQNTSIQGSPSQHSPMGTQ 302
S+ N P T G + NNP +P L +I G P ++ G +
Sbjct: 419 SIVSIKNDPKPVTCNGGFEAWYNNPKPNPNLLVGAIVGGPDEYDAYGDE 467
>At1g21080.1 68414.m02637 DNAJ heat shock N-terminal
domain-containing protein similar to SP|P39101 CAJ1
protein [Saccharomyces cerevisiae]; contains Pfam
profile PF00226 DnaJ domain;
Length = 391
Score = 27.1 bits (57), Expect = 10.0
Identities = 18/74 (24%), Positives = 31/74 (41%), Gaps = 1/74 (1%)
Frame = +3
Query: 162 AQAANMPTIGTVGQ-GAIQYVNNPMQSPQLQNTSIQGSPSQHSPMGTQSQVAKVGQGGAG 338
A+A + T+G + Q I ++P+ +L + G HS +S A G
Sbjct: 298 ARAKGLKTLGKIFQKNKIASESDPLVRAELHKLNGNGQEHDHSSTSPKSDEASRSTVGPQ 357
Query: 339 DQSSQLLSRPRLQE 380
+ S + P+L E
Sbjct: 358 EPQSPYVEAPKLGE 371
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,351,502
Number of Sequences: 28952
Number of extensions: 172709
Number of successful extensions: 431
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 424
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 430
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1246162608
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -