SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P01_F_C07
         (504 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g23290.1 68414.m02913 60S ribosomal protein L27A (RPL27aB) si...   115   2e-26
At1g70600.1 68414.m08133 60S ribosomal protein L27A (RPL27aC) id...   111   2e-25
At1g12960.1 68414.m01505 60S ribosomal protein L27A (RPL27aA) si...    44   6e-05
At2g16100.1 68415.m01846 hypothetical protein                          29   2.4  
At4g29090.1 68417.m04163 reverse transcriptase, putative / RNA-d...    27   5.4  

>At1g23290.1 68414.m02913 60S ribosomal protein L27A (RPL27aB)
           similar to 60S RIBOSOMAL PROTEIN L27A GB:P49637
           GI:1710530 from [Arabidopsis thaliana]
          Length = 146

 Score =  115 bits (276), Expect = 2e-26
 Identities = 63/149 (42%), Positives = 80/149 (53%), Gaps = 1/149 (0%)
 Frame = +1

Query: 37  MATSKKXPXKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 216
           MAT+ K   K RGHVS                 NAGG HHHRI  DKYHPGYFGK+GMR 
Sbjct: 1   MATALKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 60

Query: 217 FHFRKNKNFCPVLNLDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPK- 393
           FH  +NK FCP++NLDKLW+LV E  + K  S+ D  VP+I++ +            P+ 
Sbjct: 61  FHKLRNKFFCPIVNLDKLWSLVPEDVKAK--SSKD-NVPLIDVTQHGFFKVLGKGHLPEN 117

Query: 394 QPVIVXXXXXXXXXXXXXXDVGGACVLSA 480
           +P +V              + GGA VL+A
Sbjct: 118 KPFVVKAKLISKTAEKKIKEAGGAVVLTA 146


>At1g70600.1 68414.m08133 60S ribosomal protein L27A (RPL27aC)
           identical to 60S ribosomal protein L27A GB:P49637
           [Arabidopsis thaliana]
          Length = 146

 Score =  111 bits (268), Expect = 2e-25
 Identities = 62/149 (41%), Positives = 77/149 (51%), Gaps = 1/149 (0%)
 Frame = +1

Query: 37  MATSKKXPXKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 216
           M T  K   K RGHVS                 NAGG HHHRI  DKYHPGYFGK+GMR 
Sbjct: 1   MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 60

Query: 217 FHFRKNKNFCPVLNLDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPK- 393
           FH  +NK FCP++NLDKLW+LV E  + K  S  D  VP+I++ +            P+ 
Sbjct: 61  FHKLRNKFFCPIVNLDKLWSLVPEDVKAK--STKD-NVPLIDVTQHGFFKVLGKGHLPEN 117

Query: 394 QPVIVXXXXXXXXXXXXXXDVGGACVLSA 480
           +P +V              + GGA VL+A
Sbjct: 118 KPFVVKAKLISKTAEKKIKEAGGAVVLTA 146


>At1g12960.1 68414.m01505 60S ribosomal protein L27A (RPL27aA)
           similar to GB:BAA96068 from [Panax ginseng]
          Length = 104

 Score = 44.0 bits (99), Expect = 6e-05
 Identities = 20/45 (44%), Positives = 28/45 (62%), Gaps = 2/45 (4%)
 Frame = +1

Query: 154 HHRINMDKYHPGYFGK--LGMRNFHFRKNKNFCPVLNLDKLWTLV 282
           H R    +  PG  G   +GMR FH  +NK +C ++NLDKLW++V
Sbjct: 19  HGRFGKHRKLPGSRGNAGVGMRYFHKLRNKFYCQIVNLDKLWSMV 63


>At2g16100.1 68415.m01846 hypothetical protein 
          Length = 250

 Score = 28.7 bits (61), Expect = 2.4
 Identities = 9/19 (47%), Positives = 13/19 (68%)
 Frame = +3

Query: 150 APSQNQHGQVPSWILWQTW 206
           +P  +Q  Q+P WILW+ W
Sbjct: 20  SPRTSQFHQLPLWILWRIW 38


>At4g29090.1 68417.m04163 reverse transcriptase, putative /
           RNA-dependent DNA polymerase, putative similar to
           reverse transcriptase [Arabidopsis thaliana] GI:976278;
           contains Pfam profile PF00075: RNase H
          Length = 575

 Score = 27.5 bits (58), Expect = 5.4
 Identities = 23/77 (29%), Positives = 34/77 (44%), Gaps = 13/77 (16%)
 Frame = +3

Query: 153 PSQNQHGQVPSWILWQTWYEKFPL-*KEQEFLSSFELR*A---LDISL*TDEAEVC---- 308
           P   +  Q+  W+LW+ W  +  L  + +EF +   LR A   L+      EAE C    
Sbjct: 351 PQWEKASQLVPWLLWRLWKNRNELVFRGREFNAQEVLRRAEDDLEEWRIRTEAESCGTKP 410

Query: 309 -----ICSRWQGPRHQY 344
                 C RW+ P HQ+
Sbjct: 411 QVNRSSCGRWRPPPHQW 427


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,088,692
Number of Sequences: 28952
Number of extensions: 200240
Number of successful extensions: 502
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 483
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 500
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 898188928
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -