Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P01_F_B20
(723 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g01310.1 68418.m00043 basic helix-loop-helix (bHLH) family pr... 112 2e-25
At4g16566.1 68417.m02507 histidine triad family protein / HIT fa... 45 6e-05
At1g31160.1 68414.m03812 zinc-binding protein, putative / protei... 40 0.002
At5g11940.1 68418.m01396 subtilase family protein contains simil... 29 2.4
At3g57350.1 68416.m06384 nucleoporin interacting component-relat... 29 3.1
At2g41620.1 68415.m05143 nucleoporin interacting component famil... 29 4.1
At3g49170.1 68416.m05374 pentatricopeptide (PPR) repeat-containi... 28 5.5
At1g20510.2 68414.m02556 4-coumarate--CoA ligase family protein ... 28 7.2
At1g20510.1 68414.m02555 4-coumarate--CoA ligase family protein ... 28 7.2
At1g14840.1 68414.m01775 expressed protein 28 7.2
At5g12260.1 68418.m01441 expressed protein 27 9.5
>At5g01310.1 68418.m00043 basic helix-loop-helix (bHLH) family protein
contains Pfam profile: PF00010 helix-loop-helix
DNA-binding domain, weak hit to PF01661: Appr-1-p
processing enzyme family
Length = 912
Score = 112 bits (270), Expect = 2e-25
Identities = 64/179 (35%), Positives = 103/179 (57%), Gaps = 10/179 (5%)
Frame = +2
Query: 146 SKTPKHWSLGLIASMKDP----NSIIKNTEKVVVIKDKYPKAKVHYLVLPHEE-INSIYK 310
SK W+L L + P N +++ + +VVI D+YPKA+ H LVL +E ++ +
Sbjct: 710 SKGWNTWALALHSIAMHPERHENVVLEYLDNIVVINDQYPKARKHVLVLARQESLDGLED 769
Query: 311 LNKSHISLLEEFGNIFKELKEENESE-----LRAGFHAIPSMQRMHMHVISTDMISTSLK 475
+ K ++ LL+E N+ + + ++E R G+H++PSM+++H+HVIS D S SLK
Sbjct: 770 VRKENLQLLQEMHNVGLKWVDRFQNEDASLIFRLGYHSVPSMRQLHLHVISQDFNSDSLK 829
Query: 476 TKIHWNSFCTKFFIPYDELLQELKDIGNIRKIPSELHTSLMKTPLQCNQCSFKPKNMPE 652
K HWNSF T FF ++L+E+ G + SE L+K L+CN+C N+P+
Sbjct: 830 NKKHWNSFTTSFFRDSVDVLEEVNSQGK-ANVASE---DLLKGELRCNRCRSAHPNIPK 884
>At4g16566.1 68417.m02507 histidine triad family protein / HIT
family protein contains Pfam domain, PF01230: HIT family
(histidine triad protein family)
Length = 146
Score = 44.8 bits (101), Expect = 6e-05
Identities = 27/84 (32%), Positives = 46/84 (54%), Gaps = 6/84 (7%)
Frame = +2
Query: 206 IIKNTEKVVVIKDKYPKAKVHYLVLPHEEINSIYKLNK--SHISLLEEFGNIFKEL--KE 373
++ EKV+ +D P A+ HYLV+P E I ++ L + SL+ ++ ++L K+
Sbjct: 22 LLHTDEKVIAFQDIKPAAQRHYLVIPKEHIPTVNDLQRRDEDYSLVRHMLSVGQQLLQKD 81
Query: 374 ENESELRAGFHAIP--SMQRMHMH 439
+S R GFH P S+ +H+H
Sbjct: 82 APQSIHRFGFHQPPFNSVDHLHLH 105
>At1g31160.1 68414.m03812 zinc-binding protein, putative / protein
kinase C inhibitor, putative similar to 14 kDa
zinc-binding protein (Protein kinase C inhibitor, PKCI)
[Zea mays] Swiss-Prot:P42856
Length = 187
Score = 39.9 bits (89), Expect = 0.002
Identities = 25/97 (25%), Positives = 47/97 (48%), Gaps = 8/97 (8%)
Frame = +2
Query: 179 IASMKDPNSIIKNTEKVVVIKDKYPKAKVHYLVLP--HEEINSIYKLNKSHISLLEEFGN 352
I + + P+ I+ E V+ +D P+A VH LV+P + + S+ K H+ +L + +
Sbjct: 81 IIAKEIPSDIVYEDENVLAFRDINPQAPVHVLVIPKLRDGLTSLGKAEPRHVEVLGQLLH 140
Query: 353 IFKELKEEN------ESELRAGFHAIPSMQRMHMHVI 445
K + E+ + G A S+ +H+HV+
Sbjct: 141 ASKIVAEKEGILDGFRVVINNGVEACQSVYHLHLHVL 177
>At5g11940.1 68418.m01396 subtilase family protein contains
similarity to subtilase; SP1 GI:9957714 from [Oryza
sativa]
Length = 762
Score = 29.5 bits (63), Expect = 2.4
Identities = 19/65 (29%), Positives = 27/65 (41%)
Frame = +2
Query: 209 IKNTEKVVVIKDKYPKAKVHYLVLPHEEINSIYKLNKSHISLLEEFGNIFKELKEENESE 388
I E +V Y + KVH + L +E N + SH+ +LE K+ E
Sbjct: 19 ILKVEFNIVEGGAYEETKVHIVYLGEKEHNDPELVTSSHLRMLESLLGSKKDASESIVHS 78
Query: 389 LRAGF 403
R GF
Sbjct: 79 YRNGF 83
>At3g57350.1 68416.m06384 nucleoporin interacting component-related
contains weak hit to Pfam profile PF04097: Nucleoporin
interacting component
Length = 875
Score = 29.1 bits (62), Expect = 3.1
Identities = 18/53 (33%), Positives = 29/53 (54%)
Frame = -2
Query: 626 NIDCIAVEFSLVKYAILKESSLCYQCLLVLEVIHRRE*KTLYRNYSNVFLF*G 468
NID + V SLV ++IL E S + L V++ E ++ R Y +++L G
Sbjct: 532 NIDAVHVAISLVDHSILSEGSGTGRKLSVMDA--NAEASSMIRQYGSMYLHHG 582
>At2g41620.1 68415.m05143 nucleoporin interacting component family
protein contains Pfam profile PF04097: Nucleoporin
interacting component
Length = 861
Score = 28.7 bits (61), Expect = 4.1
Identities = 17/53 (32%), Positives = 28/53 (52%)
Frame = -2
Query: 626 NIDCIAVEFSLVKYAILKESSLCYQCLLVLEVIHRRE*KTLYRNYSNVFLF*G 468
NID + + SLV +++L E S L V++ E ++ R Y ++FL G
Sbjct: 531 NIDAVHIAISLVDHSVLSEGSGTGHKLSVMD--SNAEASSMIRQYGSMFLHHG 581
>At3g49170.1 68416.m05374 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 850
Score = 28.3 bits (60), Expect = 5.5
Identities = 12/34 (35%), Positives = 22/34 (64%)
Frame = +2
Query: 521 YDELLQELKDIGNIRKIPSELHTSLMKTPLQCNQ 622
+ LL + ++G+IRK ++H+ ++K L CNQ
Sbjct: 477 FASLLSGVANVGSIRK-GEQIHSQVVKLGLSCNQ 509
>At1g20510.2 68414.m02556 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to
SP|P14912 and SP|P14913 from Petroselinum crispum;
contains Pfam AMP-binding enzyme domain PF00501
Length = 473
Score = 27.9 bits (59), Expect = 7.2
Identities = 19/94 (20%), Positives = 44/94 (46%), Gaps = 1/94 (1%)
Frame = +2
Query: 107 LCVSAKILLPXIPSKTPKHWSLGLIASMKDPNSIIKNTEKVVVIKDKYPKAKVHYLVLPH 286
+C+S L I + P + S + +KD N ++ T ++ K K+ +++
Sbjct: 98 VCLSVMSLGAIITTTNPLNTSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDE 157
Query: 287 EEINSIYKLNK-SHISLLEEFGNIFKELKEENES 385
E ++S+ + + + E GN KE +++++
Sbjct: 158 ERVDSVGDVRRLVEMMKKEPSGNRVKERVDQDDT 191
>At1g20510.1 68414.m02555 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to
SP|P14912 and SP|P14913 from Petroselinum crispum;
contains Pfam AMP-binding enzyme domain PF00501
Length = 546
Score = 27.9 bits (59), Expect = 7.2
Identities = 19/94 (20%), Positives = 44/94 (46%), Gaps = 1/94 (1%)
Frame = +2
Query: 107 LCVSAKILLPXIPSKTPKHWSLGLIASMKDPNSIIKNTEKVVVIKDKYPKAKVHYLVLPH 286
+C+S L I + P + S + +KD N ++ T ++ K K+ +++
Sbjct: 98 VCLSVMSLGAIITTTNPLNTSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDE 157
Query: 287 EEINSIYKLNK-SHISLLEEFGNIFKELKEENES 385
E ++S+ + + + E GN KE +++++
Sbjct: 158 ERVDSVGDVRRLVEMMKKEPSGNRVKERVDQDDT 191
>At1g14840.1 68414.m01775 expressed protein
Length = 604
Score = 27.9 bits (59), Expect = 7.2
Identities = 17/57 (29%), Positives = 29/57 (50%)
Frame = +2
Query: 287 EEINSIYKLNKSHISLLEEFGNIFKELKEENESELRAGFHAIPSMQRMHMHVISTDM 457
EE+ + + K +LLE K++ EE ++ + A F A S++R+H DM
Sbjct: 95 EELGKMAEKLKLIENLLESKNLEIKKINEEKKASMAAQFAAEASLRRVHAAQKDDDM 151
>At5g12260.1 68418.m01441 expressed protein
Length = 624
Score = 27.5 bits (58), Expect = 9.5
Identities = 15/83 (18%), Positives = 36/83 (43%)
Frame = +2
Query: 314 NKSHISLLEEFGNIFKELKEENESELRAGFHAIPSMQRMHMHVISTDMISTSLKTKIHWN 493
++ + ++ F N ++ + G A P Q ++ +S+D + + + W
Sbjct: 301 SRGYFNIYTSFPNEGALSVSHRDAGVNYGRTAGPDSQLLNKSSLSSDSLKLQPLSNLKWY 360
Query: 494 SFCTKFFIPYDELLQELKDIGNI 562
FC +P +++ L ++G I
Sbjct: 361 DFCFSEVVP-GRVVRNLNELGTI 382
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,106,816
Number of Sequences: 28952
Number of extensions: 215470
Number of successful extensions: 583
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 574
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 581
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1575119672
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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